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The identification and preservative tolerance of species aggregates of Trichoderma isolated from freshly felled timber
1992 - IRG/WP 92-1553
The surface disfigurement of antisapstain treated timber by preservative-tolerant fungi remains a major problem in stored timber. Identification of a range of isolates of Trichoderma based on microscopic morphological characteristics was found to be imprecise due to the variable nature of this organism. In addition, studies to compare visual (morphological) characteristics of these isolates with t...
R J Wallace, R A Eaton, M A Carter, G R Williams


Protection of wood blocks treated with Trichoderma isolates selected on the basis of preliminary agar screening studies
1996 - IRG/WP 96-10154
Previously reported results of agar interaction screening studies for biocontrol agents of wood decay basidiomycetes showed two Trichoderma viride isolates, killed 16 of 19 target fungi (Tucker and Bruce, 1995). Testing of these isolates in wood was required to assess their performance at preventing decay of wood blocks. Standard testing of chemical wood preservatives is used to determine the toxi...
E J B Tucker, A Bruce, H J Staines


Antifungal properties of metabolites produced by Trichoderma isolates from sawdust media of edible fungi against wood decay fungi
1994 - IRG/WP 94-10051
Trichoderma isolated from a sawdust medium of Pholiota nameko produced filtrates which had antifungal activities to four wood decay fungi tested on the agar plates. Filtrates produced from another isolate, from a sawdust medium of Lentinus edodes, had antifungal activities only to the white rot fungi, Coriolus versicolor and Pycnoporus coccineus. These results did not agree with those from earlier...
S Doi, M Mori


Effect of Volatiles from Trichoderma species on the regulation of protein synthesis in Serpula lacrymans isolates
2002 - IRG/WP 02-10440
The growth of wood decay fungi has previously been shown to be inhibited by volatile organic compounds (VOCs) from selected Trichoderma isolates. No mechanism of action has however been established for such inhibition. This paper reports the effects of VOCs produced by Trichoderma pseudokoningii, T. viride and T. aureoviride on the growth of four dry rot isolates and corresponding protein synthesi...
S Humphris, R Wheatley, E Buultjens, A Bruce


Screening of bacteria, yeasts and Trichoderma isolates for antagonism toward stain and mould fungi on agar media and wood
1999 - IRG/WP 99-20159
A screening programme of soil bacteria for antagonism toward mould and wood stain fungi was undertaken on 8 types of agar-based media by application of several bacteria to each plate. The rapid screening method was subsequently verified by testing antagonism of the most promising bacteria on Scots pine sapwood. Most of the 64 bacterial showed antagonistic effects on certain media against individua...
C Payne, A Bruce


Biological control with Trichoderma harzianum in relation to the formation for spores the production of soluble metabolites
1994 - IRG/WP 94-10073
The amount of spores produced by three strains of Trichoderma harzianum on the aerial mycelium of agar cultures and in shake cultures, respectively, correlated with the inhibition zones exerted against Phanerochaete chrysosporium in an agar diffusion test. The amount of soluble antifungal metabolites as well as the protein content also correlated with the inhibition zones and the amount of spores ...
J Bürgel, E Horvath, J Haschka, K Messner


Improved equipment and technique for high pressure sap displacement impregnation of natural round wood
1972 - IRG/WP 309
Hitherto the main problem in the practical application of high pressure sap displacement impregnation (HPSD) has been in devising a satisfactory cap. Such a cap must be easily fitted to different size log ends to give a leak proof seal. The present contribution describes a new type of cap and sealing system designed to meet these requirements....
C G W Mason, F B Shorland


A new wood preservative based on polymerized complexes of aminotriazole with copper acetate
1998 - IRG/WP 98-30169
This paper presents the results of preliminary fungitoxicity tests as carried out on new polymerized complexes of aminotriazole with copper acetate (PCC) against Coniophora puteana and Trichoderma viride. Laboratory tests on wood confirmed the findings arrived at in the screening test on agar medium. Deep penetration into pine wood of compounds studied was observed and particularly so at humidity...
K J Krajewski, A Lukasiewicz, J Wazny


Decay resistance of wood removed from poles treated with Trichoderma
1989 - IRG/WP 1386
Wood blocks removed from a distribution pole previously treated with a biological control product (Binab FYT pellets) were exposed in soil block tests to selected basidiomycetes. The blocks were removed from regions of poles where Trichoderma colonization had been confirmed by extensive sampling and computer mapping of microbial inhabitants. The results indicate that material from pole interiors c...
A Bruce, B King, T L Highley


Biological control of decay in standing creosote-treated poles
1976 - IRG/WP 156
Internal decay caused by basidiomycetes in standing creosoted poles can be controlled biologically, it seems, by artificial inoculation near the ground line. For such inoculation IC-type facultative mycoparasites, Scytalidium sp FY strain or Trichoderma spp for example, may be used. Apparent residual action can be explained by the release of non water soluble chemically stable antibiotics from the...
J Ricard


A baiting technique to monitor the development and control of decay in transmission poles
1981 - IRG/WP 2149
P I Morris, D J Dickinson


Report on biological control of decay in poles
1977 - IRG/WP 149
The following its a brief report on a preliminary experiment carried out for the United Kingdoms Midland Electricity Board during 1974-1975 to establish, under laboratory conditions, the potential controlling value of some non-decay fungi against Lentinus lepideus. Lentinus lepideus has been confirmed to be the commonest Basidiomycete causing decay in poles in the Boards area....
T A Oxley


Biological control of Serpula lacrymans using Trichoderma spp
1994 - IRG/WP 94-10069
The effectiveness, or otherwise, in killing Serpula lacrymans, of a range of Trichoderma spp. in a variety of media and using two different incubation systems has been tested. In agar based systems with normal nutrients or minimal nutrients with high or low nitrogen contents and high or low iron content Trichoderma harzianum 25 proved to be the most efficient and killing Serpula lacrymans. Other s...
A J Score, J W Palfreyman


Mold inhibition on unseasoned southern pine
2003 - IRG/WP 03-10465
Concerns about indoor air quality due to mold growth have increased dramatically in the United States. In the absence of moisture management, fungicides need to be developed for indoor use to control mold establishment. An ideal fungicide for prevention of indoor mold growth on wood-based materials needs to specifically prevent spore germination and provide long-term protection under conditions of...
C A Clausen, V W Yang


Molecular studies on isolates of Serpula lacrymans
1989 - IRG/WP 1421
The major protein species present in detergent extracts of 14 different Serpula lacrymans isolates have been compared, by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), with a standard strain, viz. Serpula lacrymans FPRL 12C. Following silver staining of SDS gels the major protein species identified in 12 isolates were similar to those found in the standard strain. However ...
A Vigrow, D Button, J W Palfreyman, B King, B M Hegarty


Antagonistic effect of Trichoderma spp. against Serpula lacrymans in the soil treatment test
1991 - IRG/WP 1473
Soil treatment tests for preventing growth of Serpula lacrymans were conducted using Trichoderma spp. as antagonists. Soil specimens tested were Kanuma-soil without organic matter and the horticultural soil which was collected from the test site of the stake test. Perfect efficacy of treatment with Trichoderma spp. was shown when the horticultural soil without sterilization was used as a soil spec...
S Doi, A Yamada


The use of chlorothalonil for protection against mold and sapstain fungi. Part 1: Laboratory evaluation
1989 - IRG/WP 3515
Laboratory screening of chlorothalonil alone and in combination with other fungicides was conducted against six mold and sapstain fungi. The most promising treatments appear to be chlorothalonil supplemented with CCA or copper-8-quinolinolate. Field tests have been implemented....
J A Micales, T L Highley, A L Richter


Effect of Trichoderma harzianum on induction of laccase by Trametes versicolor on ponderosa pine sapwood
1996 - IRG/WP 96-10177
Trichoderma harzianum has long been studied as a possible biocontrol agent against wood degrading fungi, and has provided varying degrees of protection in several European field trials. In laboratory trials, however, this fungus appears to be less active against white rot fungi. This characteristic was studied using a wood wafer sandwich procedure which simultaneously expose ponderosa pine sapwood...
E A Canessa, J J Morrell


The leachability and specificity of the biological protection of timber using Scytalidium sp. and Trichoderma spp
1986 - IRG/WP 1302
The results of field experiments, using biological control against internal decay of creosoted poles, are briefly reviewed and the evidence concerning the leachability of the antibiotics produced by these species is presented. A pure culture miniblock decay test on biological control treated pine sapwood is described and the results compared to previously published data. The protection against Len...
P I Morris, N A Summers, D J Dickinson


Isolation and identification of bacteria from CCA-treated eucalypt power transmission poles
1987 - IRG/WP 1317
Bacteria have been isolated from CCA treated Eucalyptus maculata poles which possessed characteristic bacterial decay patterns. Cultures were isolated on a number of different media which had been inoculated with a splinter of degraded wood. Primary tests indicated that most isolates belonged to the genera Pseudomonas and Bacillus. The species Pseudomonas cepacia was most frequently isolated....
G A Willoughby, A C Hayward, L E Leightley


Three dimensional computer representations of growth of microbial populations in wood
1984 - IRG/WP 1243
Creosoted distribution poles inoculated with either Lentinus lepideus, biological control organisms including Triochoderma or combinations of both were extensively sampled to monitor the spread of organisms. A computer program which enabled the results to be portrayed in a three dimensional graphic form was developed and is illustrated. Results showed that computer mapping of this type usefully en...
A Bruce, B King, C Bruce, G M Smith


Modelling the control of decay in freshly felled pine poles
1997 - IRG/WP 97-10206
In a recent study investigating control of decay in freshly felled pine utility poles, it became apparent that the efficacy of different treatment methods was strongly related to the size of the material being treated. A topical application of 5% w/v disodium octaborate tetrahydrate (DOT), brush applied to the cut surfaces of logs with otherwise intact bark was just as effective at excluding white...
M W Schoeman, W Van der Werf, J F Webber, D J Dickinson


Effects of Trichoderma harzianum on enzyme activity and oxalic acid production of Gloeophyllum trabeum in ponderosa pine sapwood blocks
1992 - IRG/WP 92-1550
The effect of a bioprotectant, Trichoderma harzianum, on the activity of Gloeophyllum trabeum was investigated using a wood wafer sandwich method. Wood weight loss was greatest with Gloeophyllum trabeum exposed wafers, intermediate with those exposed to both the bioprotectant and decay fungus, and lowest with the bioprotectant exposed wafers. Extracts of wood wafers after 2, 4, or 6 weeks of funga...
C M Sexton, J J Morrell


Laboratory evaluation of chlorothalonil formulation for stain and mold control on rubberwood and maple
1998 - IRG/WP 98-30175
We evaluated the efficacy of several chlorothalonil and carbendazim fungicides (F1 and F2), etc. in the control of mold and stain fungi on rubberwood and maple. The results showed that these formulations effectively inhibited the selected fungal species such as Aspergillus niger, Penicillium sp., Trichoderma sp. (P71H), Aureobasidium pullulans, Ceratocystis minor (C-188), Ceratocystis pilifera (RW...
Mingliang Jiang, T L Highley, L Ferge, T L Woods


Effect of media composition on the antagonistic properties of Trichoderma spp. against wood decay fung
1992 - IRG/WP 92-1538
Most screening of potential biocontrol agents of wood decay fungi has previously been undertaken using artificial media. Similarly experiments designed to evaluate the mechanisms involved in antagonism between biological control agents and target fungi, have largely been carried out in conditions which do not accurately reflect the nutrient status of wood. This paper examines the influence of nutr...
U Srinivasan, A Bruce, H J Staines


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