Your search resulted in 62 documents. Displaying 25 entries per page.
Biological control with Trichoderma harzianum in relation to the formation for spores the production of soluble metabolites
1994 - IRG/WP 94-10073
The amount of spores produced by three strains of Trichoderma harzianum on the aerial mycelium of agar cultures and in shake cultures, respectively, correlated with the inhibition zones exerted against Phanerochaete chrysosporium in an agar diffusion test. The amount of soluble antifungal metabolites as well as the protein content also correlated with the inhibition zones and the amount of spores ...
J Bürgel, E Horvath, J Haschka, K Messner
The use of chlorothalonil for protection against mold and sapstain fungi. Part 1: Laboratory evaluation
1989 - IRG/WP 3515
Laboratory screening of chlorothalonil alone and in combination with other fungicides was conducted against six mold and sapstain fungi. The most promising treatments appear to be chlorothalonil supplemented with CCA or copper-8-quinolinolate. Field tests have been implemented....
J A Micales, T L Highley, A L Richter
Effect of Trichoderma harzianum on induction of laccase by Trametes versicolor on ponderosa pine sapwood
1996 - IRG/WP 96-10177
Trichoderma harzianum has long been studied as a possible biocontrol agent against wood degrading fungi, and has provided varying degrees of protection in several European field trials. In laboratory trials, however, this fungus appears to be less active against white rot fungi. This characteristic was studied using a wood wafer sandwich procedure which simultaneously expose ponderosa pine sapwood...
E A Canessa, J J Morrell
The leachability and specificity of the biological protection of timber using Scytalidium sp. and Trichoderma spp
1986 - IRG/WP 1302
The results of field experiments, using biological control against internal decay of creosoted poles, are briefly reviewed and the evidence concerning the leachability of the antibiotics produced by these species is presented. A pure culture miniblock decay test on biological control treated pine sapwood is described and the results compared to previously published data. The protection against Len...
P I Morris, N A Summers, D J Dickinson
Effects of Trichoderma harzianum on enzyme activity and oxalic acid production of Gloeophyllum trabeum in ponderosa pine sapwood blocks
1992 - IRG/WP 92-1550
The effect of a bioprotectant, Trichoderma harzianum, on the activity of Gloeophyllum trabeum was investigated using a wood wafer sandwich method. Wood weight loss was greatest with Gloeophyllum trabeum exposed wafers, intermediate with those exposed to both the bioprotectant and decay fungus, and lowest with the bioprotectant exposed wafers. Extracts of wood wafers after 2, 4, or 6 weeks of funga...
C M Sexton, J J Morrell
Transformation of Ophiostoma picea and Trichoderma harzianum with green fluorescent protein (GFP)
2003 - IRG/WP 03-10477
While microbial colonization of wood is presumed to be characterized by a myriad of interactions between numerous organisms, studying these processes is often difficult owing to the opaque nature of the wood and the inability to readily distinguish among the many species colonizing the material. One method for enhancing the ability to distinguish organisms is to induce specific proteins in one or ...
Ying Xiao, L M Ciuffetti, J J Morrell
Effect of nutrient regimes, temperature, pH, and wood sterilization method on performance of selected bioprotectants against wood staining fungi
1992 - IRG/WP 92-1551
The effect of nutrient regimes, incubation temperature, media pH, and wood sterilization method on performance of four potential bioprotectants (Pseudomonas putida, Pseudomonas cepacia, Bacillus subtilis, and Trichoderma harzianum) against wood staining fungi were evaluated using small ponderosa pine samples over a four week period. Incubation at 32°C resulted in slight increases in the degree of...
J J Morrell, C M Secton
Preliminary evaluations of a small wafer assay for screening potential biological control agents
1989 - IRG/WP 2332
Screening potential biological agents for controlling wood decay fungi poses a dilemma. The ideal test would eliminate as many variables as possible. Most tests utilize pure cultures of the test organism on an artificial media which in no way resembles wood. The use of sawdust improves this approach, but the sawdust increases fungal access to the lignocellulose matrix, potentially inflating the im...
C M Freitag, J J Morrell
Effects of Prior Establishment of Trichoderma harzianum on Ophiostoma picea Growth in Freshly Sawn Douglas-fir Sapwood
2003 - IRG/WP 03-10476
Trichoderma harzianum has been shown to be an effective biocontrol agent against a number of wood inhabiting fungi under laboratory conditions, but this fungus has performed poorly in field trials. Understanding the interactions between biocontrol agents and their intended targets in wood may provide important clues for developing improved approaches to biocontrol, potentially reducing our relianc...
Ying Xiao, J J Morrell, L M Ciuffetti
Application of DNA fingerprinting methods to identify biocontrol strains of fungi imperfecti
1994 - IRG/WP 94-10068
We have analyzed a number of biocontrol strains of Trichoderma harzianum and other Trichoderma strains with the methods DNA fingerprinting and PCR fingerprinting to differentiate and identify these strain which is not possible with morphological or biochemical methods. We could differentiate even gamma-ray induced mutants from each other as well as different strains form the same and different spe...
A Schlick, K Kuhls, W Meyer, E Lieckfeld, T Börner, K Messner
Biological control of wood decay fungi. - Part II. Effects of exogenous nitrogen on effectiveness
2000 - IRG/WP 00-10360
The effects of nitrogen (urea and ammonium nitrate) on the ability of Trichoderma harzianum to inhibit the enzyme capabilities and decay capacities of Trametes versicolor and Gloeophyllum trabeum were investigated using a small wood sandwich decay test. The biocontrol fungus generally had a greater effect on the brown rot fungus, confirming previous reports. Nitrogen produced inconsistent effects ...
E A Canessa, J J Morrell
Effects of chemical pretreatment of Douglas-fir heartwood on efficacy of potential bioprotection agents
1990 - IRG/WP 1440
Biological protection against wood decay fungi represents an environmentally attractive alternative to the use of chemicals; however, the process of identifying suitable candidates and the conditions that ensure successful protection pose major challenges. One critical aspect of this problem is to identify organisms that can rapidly and uniformly colonize the wood. Wood contains low levels of nutr...
B Dawson-Andoh, J J Morrell
The role of chitinase in bioprotectant activity against staining fungi
1996 - IRG/WP 96-10175
Chitin is an important structural component of the hyphae of many wood staining fungi and its disruption can lead to dramatic declines in their growth. A number of bioprotectants have been shown to produce chitinases in liquid cultures, but the role of these enzymes in bioprotection remains poorly understood. The levels of these enzymes was studied by inoculating ponderosa pine sapwood wafers with...
J Liu, J J Morrell
The effect of chitosan on the growth and physiology of two wood inhabiting fungi
2006 - IRG/WP 06-10590
The effect of chitosan on the wood inhabiting fungi Sphaeropsis sapinea and Trichoderma harzianum was evaluated at cellular level. Increasing concentrations of chitosan caused an increase in the amount of peroxide in cultures of S. sapinea, which was accompanied by a simultaneous decrease in superoxide. The same effect was not observed in T. harzianum. The growth of both fungi was inhibited when h...
D Vesentini, T Singh
Effect of chitosan on the morphology and ultrastructure of two wood inhabiting fungi
2007 - IRG/WP 07-10606
An investigation was undertaken to compare the antifungal effects caused by chitosan against two selected wood degrading fungi Sphaeropsis sapinea and Trichoderma harzianum on their growth, morphological and ultra-structural characteristics, using differential interference microscopy, epifluorescence microscopy and transmission electron microscopy.
The effect of chitosan on fungal morphology was ...
T Singh, A P Singh, G Daniel
In vitro evaluation of an integrated approach using Trichoderma harzianum and chitosan for the control of sapstain
2008 - IRG/WP 08-10659
In vitro assays were undertaken to evaluate the control of two sapstain fungi, Leptographium procerum and Sphaeropsis sapinea by a combination of chitosan or chitosan oligomer (CODP-14) and a naturally mutated strain of Trichoderma harzianum. Spore germination and hyphal growth of the test fungi were assessed on media amended with chitosan or chitosan oligomer with and without T. harzianum using ...
C Chittenden, T Singh
Biological control of the sapstain fungus Diplodia pinea (syn. Sphaeropsis sapinea) in the field
2008 - IRG/WP 08-10663
Diplodia pinea, syn. Sphaeropsis sapinea, is well known as the worldwide ocurring causal agent of the Diplodia tip-blight disease on various pine species, leading to great losses of valuable wood in the forests. Moreover, this fungus is also responsible for the fornation of sapstain of felled logs, leading to undesired disation of surface layers in the OSB manufacturing industry. In Central Europe...
C Gradinger, T Boisselet, K Messner, K Fackler
Improved equipment and technique for high pressure sap displacement impregnation of natural round wood
1972 - IRG/WP 309
Hitherto the main problem in the practical application of high pressure sap displacement impregnation (HPSD) has been in devising a satisfactory cap. Such a cap must be easily fitted to different size log ends to give a leak proof seal. The present contribution describes a new type of cap and sealing system designed to meet these requirements....
C G W Mason, F B Shorland
A new wood preservative based on polymerized complexes of aminotriazole with copper acetate
1998 - IRG/WP 98-30169
This paper presents the results of preliminary fungitoxicity tests as carried out on new polymerized complexes of aminotriazole with copper acetate (PCC) against Coniophora puteana and Trichoderma viride.
Laboratory tests on wood confirmed the findings arrived at in the screening test on agar medium. Deep penetration into pine wood of compounds studied was observed and particularly so at humidity...
K J Krajewski, A Lukasiewicz, J Wazny
Decay resistance of wood removed from poles treated with Trichoderma
1989 - IRG/WP 1386
Wood blocks removed from a distribution pole previously treated with a biological control product (Binab FYT pellets) were exposed in soil block tests to selected basidiomycetes. The blocks were removed from regions of poles where Trichoderma colonization had been confirmed by extensive sampling and computer mapping of microbial inhabitants. The results indicate that material from pole interiors c...
A Bruce, B King, T L Highley
The identification and preservative tolerance of species aggregates of Trichoderma isolated from freshly felled timber
1992 - IRG/WP 92-1553
The surface disfigurement of antisapstain treated timber by preservative-tolerant fungi remains a major problem in stored timber. Identification of a range of isolates of Trichoderma based on microscopic morphological characteristics was found to be imprecise due to the variable nature of this organism. In addition, studies to compare visual (morphological) characteristics of these isolates with t...
R J Wallace, R A Eaton, M A Carter, G R Williams
Biological control of decay in standing creosote-treated poles
1976 - IRG/WP 156
Internal decay caused by basidiomycetes in standing creosoted poles can be controlled biologically, it seems, by artificial inoculation near the ground line. For such inoculation IC-type facultative mycoparasites, Scytalidium sp FY strain or Trichoderma spp for example, may be used. Apparent residual action can be explained by the release of non water soluble chemically stable antibiotics from the...
J Ricard
A baiting technique to monitor the development and control of decay in transmission poles
1981 - IRG/WP 2149
P I Morris, D J Dickinson
Report on biological control of decay in poles
1977 - IRG/WP 149
The following its a brief report on a preliminary experiment carried out for the United Kingdoms Midland Electricity Board during 1974-1975 to establish, under laboratory conditions, the potential controlling value of some non-decay fungi against Lentinus lepideus. Lentinus lepideus has been confirmed to be the commonest Basidiomycete causing decay in poles in the Boards area....
T A Oxley
Biological control of Serpula lacrymans using Trichoderma spp
1994 - IRG/WP 94-10069
The effectiveness, or otherwise, in killing Serpula lacrymans, of a range of Trichoderma spp. in a variety of media and using two different incubation systems has been tested. In agar based systems with normal nutrients or minimal nutrients with high or low nitrogen contents and high or low iron content Trichoderma harzianum 25 proved to be the most efficient and killing Serpula lacrymans. Other s...
A J Score, J W Palfreyman