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Mould growth at lumber surfaces of pine after kiln and air drying
1994 - IRG/WP 94-40033
Distribution of water soluble substances in green wood and later redistribution during drying is of importance for the colonisation of wood by microorganisms. According to literature the availability of nitrogenous materials is probably a major limiting factor to the microbial colonisation of wood. King et al. (1974) and Oxley et al. (1976) have shown correlation between surface nutrient concentra...
N Terziev, J Bjurman, J B Boutelje


Use of compression strength loss for measuring decay in the soil block test
1996 - IRG/WP 96-20083
The possibility of using radial direction compression strength of wood, rather than mass loss, was evaluated for both a brown-rot fungus (Gloeophyllum trabeum) and white-rot fungus (Trametes versicolor). With untreated pine wafers (5 x 19 x 19 mm³ - l x r x t) exposed to Gloeophyllum trabeum in a soil block test for five days, the compression strength loss was nearly 50% compared to a 5% mass los...
D D Nicholas, Zhongwei Jin


Effect of crude tall oil, linseed oil and rapeseed oil on the growth of the decay fungi
2002 - IRG/WP 02-30299
The influence of crude tall oil, linseed oil and rapeseed oil on the growth of Coniophora puteana, Poria placenta and Coriolus versicolor was studied. The selected test oils were observed to have different effects on the the fungal growth. Crude tall oil inhibited the radial growth of all fungi. Rapeseed oil either accelerated or inhibited the growth of fungi depending on the type of fungus involv...
L Paajanen, A-C Ritschkoff


Screening wood preservatives: Comparison of the soil block, agar block and agar plate tests
1993 - IRG/WP 93-20001
Several test procedures have been developed over the years to screen potential biocides for their value as wood preservatives. Each test has inherent advantages and disadvantages. In this paper the relative merits of the soil block, agar block and agar plate tests are compared. Eight commercially available biocides encompassing inorganic and organic systems were tested against four basidiomycete d...
K J Archer, D D Nicholas, T Schultz


Biological control of wood decay fungi. - Part II. Effects of exogenous nitrogen on effectiveness
2000 - IRG/WP 00-10360
The effects of nitrogen (urea and ammonium nitrate) on the ability of Trichoderma harzianum to inhibit the enzyme capabilities and decay capacities of Trametes versicolor and Gloeophyllum trabeum were investigated using a small wood sandwich decay test. The biocontrol fungus generally had a greater effect on the brown rot fungus, confirming previous reports. Nitrogen produced inconsistent effects ...
E A Canessa, J J Morrell


Extracellular mucilage (ECM) in wood decay basidiomycetes
2002 - IRG/WP 02-10439
The ability of wood decay basidiomycetes to produce extracellular mucilage (ECM) and its relationship with total biomass production is being investigated. Growth and ECM production by the brown-rot fungus Gloeophyllum trabeum (FPRL 108 N) and the white-rot fungus Coriolus versicolor (CTB 863 A) was assessed in liquid culture under different conditions and in the presence of the fungicide cyprocona...
D Vesentini, D J Dickinson, R J Murphy


Development of threshold values for boron compounds in above ground exposures: Preliminary trials
1998 - IRG/WP 98-30179
Boron is increasingly used both as an initial and remedial treatment for protecting wood against fungal and insect attack. While establishing lethal dosages for boron against insects through feeding tests is relatively simple, establishing thresholds for fungal attack poses more of a challenge. Tests using boron dispersed in agar artificially surround the fungus with both boron and excess nutrient...
J J Morrell, C M Freitag, S Unger


Fungal detoxification of organotin biocides
1985 - IRG/WP 1258
The ability of a range of wood decaying fungi to inactivate bis(tri-n-butyltin) oxide (TnBTO) in the extracellular growth medium, in stationary liquid culture was determined. A distinction between the ability to tolerate the fungicide and to inactivate it was made: the white-rot organism Coriolus versicolor being the most efficient inactivator. In an attempt to determine the extracellular agents r...
P S Belford, D J Dickinson


Cation analysis of wood degraded by white and brown rot fungi
1992 - IRG/WP 92-1552
Changes in the concentrations and composition of cations within wood colonized by the brown-rot fungi Postia placenta and Gloeophyllum trabeum and the white-rot fungi Trametes versicolor and Phanaerochaete chrysosporium were analyzed using plasma emission and atomic absorption spectrophotometry. In degraded poplar wood significant decreases in pH and electrical resistance readings were observed as...
J Jellison, K C Smith, W T Shortle


A biochemical explanation for the observed patterns of fungal decay in timber
1980 - IRG/WP 1111
Experiments designed to compare the degree of localization of the cellulase enzymes of some white, brown and soft rot organisms are described. The site and nature of binding of the enzymes is discussed. The technique is ellution of mycelium grown in liquid culture with a variety: of agents including acetate buffer, carboxymethyl cellulose solution, borate/glycerol buffer and urea. The mycelium was...
N B Green, D J Dickinson, J F Levy


Effects of chemical pretreatment of Douglas-fir heartwood on efficacy of potential bioprotection agents
1990 - IRG/WP 1440
Biological protection against wood decay fungi represents an environmentally attractive alternative to the use of chemicals; however, the process of identifying suitable candidates and the conditions that ensure successful protection pose major challenges. One critical aspect of this problem is to identify organisms that can rapidly and uniformly colonize the wood. Wood contains low levels of nutr...
B Dawson-Andoh, J J Morrell


Co-operative studies on determining toxic values against wood-destroying basidiomycetes: Progress report to April 1990
1990 - IRG/WP 2357
This document reports progress on the co-operative study between nine laboratories, set up following the proposals contained in Document IPG/WP/2316 (1988). Results have been received from eight laboratories. Toxic values data have been established successfully for the test fungus Coniophora puteana with soil, malt agar and vermiculite methods and with the test fungi Gloeophyllum trabeum and Poria...
J K Carey, A F Bravery


Biological resistance of electrolessly plated wood (1). Preliminary report
1994 - IRG/WP 94-40024
Surface metal coating is considered to improve biological resistance of wood as some metal ions are fungitoxic. Six wood species (three softwoods: Cryptomeria japonica, Tsuga heterophylla and Larix spp; three hardwoods: Fagus crenata, Acer mono and Betula platyphylla) were electrolessly plated with nickel or copper, and those were served for laboratory evaluation of their resistance against decay ...
M Hasegawa, K Tsunoda, T Yoshimura


Method to determine the depth of penetration of the biologically active components of wood preservatives
1978 - IRG/WP 2108
A time-saving method for determining the depth of penetration of the biologically active components of wood preservatives is described. The test specimens were obtained by cutting thin slices from the wood either parallel or perpendicular to the treated surface. The slices were then exposed to fungal attack. A good correlation was found between the test results obtained by the modified German Stan...
H-P Sutter


Uptake of copper by mycelium of wood decay fungi growing on copper S-substituted thioglycolate containing nutrient media
1998 - IRG/WP 98-10291
Mycelia of Trametes versicolor, Coniophora puteana and Poria monticola were grown on potato dextrose agar (PDA) media, containing various concentrations of copper N,N-dimethyldithiocarbamoylacetate (which may be regarded as a copper S-substituted thioglycolate). The tested copper compound revealed relatively low fungicidal activity. After 13-18 day growing period, we determined concentrations of a...
M Humar, M Petric, F Pohleven, P Kalan


Use of immunoblotting for the analysis of wood decay basidiomycetes
1988 - IRG/WP 2307
Immunoblotting methods, in particular dot and Western blotting have been used to investigate features of a variety of wood decay organisms, in particular Coriolus versicolor, Lentinus lepideus and Serpula lacrymans. Antisera to each of these decay organisms has been produced by immunisation of rabbits with liquid culture grown hyphae. These antisera, after appropriate preabsorption with sawdust ha...
J W Palfreyman, H Glancy, D Button, A Vigrow, A J Score, B King


Biological resistance of furfuryl alcohol-treated wood
1992 - IRG/WP 92-3703
Biological resistance of FFA (furfuryl alcohol)-treated wood was investigated in relation to the rise of ASE (anti-swelling efficiency) resulting from the treatment. Sapwood blocks of Japanese red pine, cedar and beech were vacuum-impregnated with various concentrations of aqueous FFA solutions to get the target levels of% (w/w) RL (resin loading). Air-dried blocks were kept in an oven at 120°C f...
J Y Ryu, Y Imamura, M Takahashi


Effects of biocides on the extracellular mucilaginous material (ECMM) produced by two wood rotting basidiomycetes
2003 - IRG/WP 03-10469
Growth and production of extracellular mucilaginous material (ECMM) by the brown-rot fungus Gloeophyllum trabeum (FPRL 108 N) and the white-rot fungus Coriolus versicolor (CTB 863 A) was assessed in liquid culture, supplemented with the biocides CuSO4 and cyproconazole. The production of biomass in G. trabeum was significantly influenced by the concentration of CuSO4 in the medium. When CuSO4 was...
D Vesentini, D J Dickinson, R J Murphy


The efficacy of boron preparations
1987 - IRG/WP 3400
The toxicity threshold of boric acid for egg larvae of Hylotrupes bajulus Linné is approx. 0.4 kg/m³ of wood (71 g B/m³) over a 12-week test period, and approx. 0.3 kg/m³ of wood (53 g B/m³) over a 6-month test period. The corresponding values for borax are 0.5-0.6 kg/m³ of wood (56-68 g B/m³) and 0.3-0.4 kg/m³ of wood (34-45 g B/m³). The values are somewhat lower in the case of the egg l...
J T De Jonge


Production of fungal protoplasts from selected wood-degrading fungi
1991 - IRG/WP 1500
Studies of hyphal morphology and the effects of various chemicals on growth are often difficult to perform on filamentous fungi because of the difficulty of observing the protoplasm through the rigid hyphae wall and because most activity occurs in a limited region near the hyphal tip. As an alternative, hyphae can be reacted with certain cell wall degrading enzymes to remove the cell wall to produ...
C Rui, J J Morrell


The resistance of wood coated with different water-borne paints against colonisation by decay fungi
1996 - IRG/WP 96-10165
The susceptibility of wood painted with model paints of known composition to decay fungi was tested without previous weathering. Included in the study were five alkyd emulsion paints and five acrylic paints; one linseed oil paint and two solvent-borne alkyd paints. It was found that several components influenced the susceptibility of these paints. The results of the present study indicate that the...
J Bjurman


Diazenes and some organic complexes of boron as potential fungicides for preservation of wood
1999 - IRG/WP 99-30197
Screening for fungicidal activity represents the first step in searching for new active components with enhanced environmental profile in comparison with traditional wood preservatives. Diazenes, their salts, and the salts of the corresponding semicarbazides, as well as several complexes of boron, were screened for fungicidal activity against wood decay fungi Trametes versicolor, Coniophora putean...
M Petric, B Paradiz, J Stern, F Pohleven, S Polanc, B Stefane, R Lenarsic


The effect of woody and non woody plants extractives on microbial resistance of non-durable species
2006 - IRG/WP 06-30392
The effect of Elm (Zelkova carpinifolia), Oak (Quercus castanifolia), Mulberry (Morus alba), Ash (Fraxinus excelsior) as woody plants, Rose (Rosa damascene) and Fumitory (Fumaria sp) as non woody plants extractives on durability of Beech (Fagus orientalis), Maple (Acer insgin), Alder (Alnus subcordata), and Lime (Tilia sp) were studied. First wood species having extractives were cut to small piece...
S M Kazemi, A Hosinzadeh, M B Rezaii


The influence of wood density on the durabilities of three Ghanaian hardwoods (Nauclea diderrichii (de Wild.) Merr., Nesogordonia papaverifera (A. Chev.) R. Capuron and Corynanthe pachyceras Welw.) )
2007 - IRG/WP 07-10603
Review of factors that influence wood durability shows that although density varies depending on stem position, its role on decay resistance appears controversial. The role density plays in influencing the durability of three hardwoods from Ghana, namely Nauclea diderrichii (de Wild.) Merr., Nesogordonia papaverifera (A. Chev.) R. Capuron) and Corynanthe pachyceras Welw. is therefore investigated ...
C Antwi-Boasiako, A J Pitman, J R Barnett


The characteristics of biodegradation mechanism of gamma-irradiated wood
2007 - IRG/WP 07-20352
Gamma radiation, as an easy, fast and effective sterilisation method, changes molecular structure in pest’s living cells, but also in wooden cell walls. Radiation-induced depolymerisation causes significant changes in wood properties crucial for laboratory testing of wood-preservative effectiveness. Natural durability of wood against rot fungi in lab conditions dependency on different sterilisat...
R Despot, M Hasan, F Pohleven, M Humar, G Rep


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