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Biological control of sapwood-inhabiting fungi by living bacterial cells of Streptomyces rimosus as a bioprotectant
1992 - IRG/WP 92-1564
The objective of this study is to evaluate the efficacy of antifungal activity of living bacterial cells for the protection of wood against sapwood-inhabiting fungi. The following sapwood-inhabiting fungi were selected: sapstain --Ceratocystis coerulescens, Ceratocystis minor, Ceratocystis pilifera, and Aureobasidum pullulans; mold fungi --Aspergillus niger, Penicillium spp, and Trichoderma spp. L...
S C Croan, T L Highley


Comparative study of blue stain resistance of various types of wood stains after artificial and natural weathering
1992 - IRG/WP 92-2411
For the determination of the protective effectiveness of a preservative treatment against blue stain in service, artificial weathering has been proposed as an alternative for the natural weathering period of 6 months in the European standard EN 152. Research on a range of products and on complete finishing systems for external joinery was conducted during 1986-1990. It revealed that the decisions ...
J Van Acker, M Stevens, M Nys


The influence of timber species and preservative treatment on spore germination of some wood-destroying Basidiomycetes
1988 - IRG/WP 2300
Basidiospores from six wood decay fungi exhibited varying germination rates on untreated softwood and hardwood blocks. Germination inhibition of all test fungi was recorded on pine sapwood. No preference for a certain timber species by a particular fungus was evident. Whereas almost complete inhibition of germination occurred on wood treated with a quarternary-ammonium based wood preservative, mos...
B M Hegarty, G Buchwald


Wood preservation in Japan
1982 - IRG/WP 3218
The report indicates the importance of wood preservation in Japan, which is the world's largest importer. The main hazards of timber are listed, fungi, insects and marine borers. Besides wood preservation fire protection treatments are also important in Japan. Classifications are given of the durability and treatability of the timbers used. In describing the wood preservation industry, th...
S Amemiya, R Cockcroft


Partial characterization of inhibitors extracted from pine (Pinus sylvestris) sapwood active against germination of wood rotting fungi
1988 - IRG/WP 1351
Germination inhibitors in pine sapwood could be extracted and separated with the aid of solvent partitioning and chromatography on silicic acid. The inhibitory action was tested by three different bioassays. Active fractions have been characterized by TLC and HPLC. Inhibitory activity could be correlated with phenolic compounds. The minimum active concentration of inhibitors have been determined....
J Bjurman


Influence of the content of organic matter of soil on the degradation of wood by soft rot fungi
1970 - IRG/WP 27
The degradation of wood specimen burried in the soil by soft rot fungi is influenced by a number of factors. These are in particular the water holding capacity, water content, content of organic matter, pH, content of mineral nutritive substances etc. The results of the interlaboratory tests, carried out within the IRG/WP let presume that the intensity of wood degradation occurs proportionally inv...
O Wälchli


Isolation of a gene from the melanin pathway of the sapstaining fungi Ophiostoma piceae using PCR
1997 - IRG/WP 97-10219
To prevent sapstaining fungi from discoloring wood, it is necessary to determine what factors affect the biosynthesis and characteristics of the pigment(s) and to identify the genes involved in the pathway. Using inhibitors and heterologous DNA probes from Alternaria alternata, we suggest that melanin, the pigment of Ophiostoma piceae, is produced by the dihydroxynaphthalene (DHN) pathway. Recentl...
R Eagen, J Kronstad, C Breuil


The study of blue stain found in coniferous timber in SR Slovenia (Yugoslavia)
1983 - IRG/WP 1177
The report concerns the problems connected with blue stain in coniferous timbers stored in warehouses at wood-processing plants in SR Slovenia (Yugoslavia). The amounts of spruce-wood (Picea abies Karst.) found to have been infected with blue stain have been measured and some of the fungi causing the stain have been identified and their biology studied. It was found that the percentage of spruce-w...
R Benko


Degradation of woods by obligately anaerobic fungi
1990 - IRG/WP 1452
Little is known about the processes occurring during the degradation of wood by obligately anaerobic fungi. When woods from a range of hardwood species were incubated for 11 days with a strain of the Chytrid Neocallimastix frontalis many of the woods were measurably fermented; Fagus sylvatica (23%) was the most highly degraded. Microscopic examination of Populus tremuloides wood after incubation w...
K N Joblin, G E Naylor


A Novel Type of Multiple Cavity Attack in Wood Cell Walls of Heat-treated Timber Exposed in Seawater – Preliminary Observations
2004 - IRG/WP 04-10523
Samples of untreated and heat-treated Norway spruce and Douglas fir were submerged in Langstone Harbour, Portsmouth for 4 years at a depth of ca. 0.3 metres. The heat-treated samples had been prepared using the Plato process. Samples were initially assessed for the severity of marine borer damage and were then examined microscopically for evidence of microbial decay. Longitudinal and transverse wo...
R A Eaton, C Björdal, T Nilsson


Effects of pre-harvest girdling on selected properties of red pine, red maple and Eastern larch
1999 - IRG/WP 99-40137
Trees of three species - tamarack (Larix laricina), soft maple (Acer rubrum) and red pine (Pinus resinosa) - were girdled immediately below the crown,one to two years before felling. The wood from these trees was compared with wood taken from un-girdled control trees felled at the same. Sapwood and heartwood, from the controls, and from above and below the girdle of treated trees, were examined fo...
A Taylor, P A Cooper


Extracellular carbohydrate production by isolates of Postia (=Poria) placenta
1989 - IRG/WP 1388
A monokaryotic strain of Postia (=Poria) placenta, ME20, which is unable to degrade wood, also failed to produce extracellular polysaccharide when grown in liquid culture, regardless of carbon source or concentration. Other isolates of Postia placenta, including another monokaryon and a hybrid of this monokaryon with ME20, produced large quantities of this material. The polysaccharide consisted pr...
J A Micales, A L Richter, T L Highley


Moisture Adsorption Behaviour of Rubber Wood Decayed by Brown and White rot Fungi
2005 - IRG/WP 05-40306
Moisture adsorption behaviour of rubber wood blocks decayed by each brown rot and white rot fungi was studied. The adsorption isotherms of decayed wood were compared with the isotherm of undecayed wood. The wood decayed by brown rot adsorbed less moisture as compared to sound or undecayed wood at all humidity levels. No significant difference was observed in adsorption behaviour of white rot dec...
S S Chauhan, H C Nagaveni


Movement of chloropicrin, Vapam, and methylisothiocyanate in southern pine and Douglas fir timbers
1987 - IRG/WP 3410
Douglas fir and southern pine timbers, 15.2 x 15.2 x 426 cm³ (6-in x 6-in x 14-ft), were "inoculated" with brown-rot and white-rot fungi as vapor-sensing agents to evaluate the movement and distribution of fungitoxic concentrations of chloropicrin, Vapam, and methylisothiocyanate (MIT) over a 20-week period. Residual fumigant in timbers was determined by a bioassay with Gloeophyllum trabeum. The ...
T L Highley


Pretreatment decay in poles of Pinus sylvestris
1987 - IRG/WP 1329
Storage damage by blue stain and decay in poles before impregnation has been increasingly observed during recent years and there have been reports that salt-impregnated poles today are of lower quality because of incipient decay being increasingly common. The attacks may have started at the time of cutting and then continued during the period of seasoning at the plant, which could be as long as tw...
H Lundström, M-L Edlund


Why don't more people work with Serpula lacrymans nowadays? A discussion of some of the different approaches to experimenting with this unique fungus
1989 - IRG/WP 1383
Remarkably few laboratories are currently investigating this well-known fungus. Not only has it failed to gain acceptance as a standard test organism (for wood preservative evaluation) in many countries, but is also sometimes excluded from pure research studies when it ought to be an obvious first choice as a representative basidiomycete. This paper discusses some different approaches that have be...
J D Thornton


Antagonism of Gliocladium virens against wood decay fungi
1995 - IRG/WP 95-10102
Antagonistic abilities of a commercial biofungicide, Gliocladium virens (GL-21, Grace) were evaluated against three white-rot fungi, Trametes versicolor, Phlebia brevispora, Irpex lacteus, and three brown-rot fungi, Postia placenta, Neolentinus lepideus, Gloeophyllum trabeum. In dual culture of Gliocladium virens and wood decay fungi, Gliocladium virens rapidly overgrow the decay fungi and killed ...
T L Highley, L Ferge


Characterization and differentiation of wood rotting fungi by protein and enzyme patterns
1999 - IRG/WP 99-20177
Standardized tests for wood preservatives are performed with defined fungal strains to ensure comparability between laboratories. However, changes of virulence and variation of results are well known events. Suitable and reliable measures to control the stability of the test organisms are necessary.Comparison of protein patterns produced by SDS-electrophoresis was already described by several auth...
U Schoknecht


Antagonistic effects of Dacrymyces stillatus against growth of other fungi
1991 - IRG/WP 2363
The antagonistic effects in dual cultures on malt extract agar of 5 wood decay fungi and the antagonistic effects of Dacrymyces stillatus against 1 mould fungus and 1 blue stain fungus were determined. Dacrymyces stillatus was the only fungus which exhibited clear antagonistic effects. The effects of Dacrymyces stillatus against the different test fungi varied however to a great extent. Dacrymyces...
J Bjurman


A comparison of fatty acid and molecular profiles for identification of wood colonizing basidiomycota
2003 - IRG/WP 03-20278
Two methods that are currently being employed to detect and identify wood decay fungi are Fatty Acid Methyl Ester (FAME) analysis and Restriction Fragment Length Polymorphism (RFLP) analysis. A FAME library and RFLP library for 9 species and up to 10 strains within each species have been developed. The profiles generated by these methods have been compared for each species and strain to test for...
T C McElroy, L Prewitt, S V Diehl


Production of fungal protoplasts from selected wood-degrading fungi
1991 - IRG/WP 1500
Studies of hyphal morphology and the effects of various chemicals on growth are often difficult to perform on filamentous fungi because of the difficulty of observing the protoplasm through the rigid hyphae wall and because most activity occurs in a limited region near the hyphal tip. As an alternative, hyphae can be reacted with certain cell wall degrading enzymes to remove the cell wall to produ...
C Rui, J J Morrell


Chitin synthetase activity in cellular fractions of wood-decay fungi
1992 - IRG/WP 92-1524
This study was an attempt to explain the variability of in vivo sensitivity of wood decay fungi to the chitin synthetase (CS) inhibitor, polyoxin, by comparing CS distribution in cellular fractions. The five species studied were the brown-rot fungi Coniophora puteana, Gloeophyllum trabeum, Fibuloporia vaillantii, and the white-rot fungi Schizophyllum commune and Ganoderma applanatum. The CS activi...
B R Johnson, S C Croan, B Illman


Influence of fungal exposure on the redistribution of copper in treated spruce wood
2002 - IRG/WP 02-10450
The redistribution of copper in treated wood after exposure to basidiomycete decay fungi is described. The micro-distribution of copper in copper(II) sulphate or copper(II) octanoate/ethanolamine treated Norway spruce wood before and after exposure to 3 different wood decay fungi was studied using transmission electron microscopy with X-ray microanalysis. The copper content of the mycelium and the...
M Humar, F Pohleven, R J Murphy, D J Dickinson, I Moris, M Zupancic, P Kalan, M Petric


The effect of stack height on the performance of preservatives used for the prevention of sapstain on seasoning wood
1997 - IRG/WP 97-10192
The performance of three anti-sapstain preservatives was investigated under field conditions in south east England. The preservatives under test were: copper-8-quinolinolate (Mitrol PQ8) trimethylalkylammoniumchloride and sodium hexanoate (Sinesto B) and chlorothalonil (Tuff Brite). The preservatives were tested on Corsican Pine grown in south east England. The field trial was set up to investigat...
J R Williams, D J Dickinson, J F Webber


Evaluation of fungi isolated from the galleries of the striped ambrosia beetle Xyloterus lineatus (Ol.) (Col., Scolytidae)
1995 - IRG/WP 95-10092
Fungi from the galleries of the striped ambrosia beetle Xyloterus lineatus (Trypodendron lineatum) (Ol.) found in spruce logs were studied. The following fungi were isolated and identified: Trichoderma harzianum Rifai, the yeast Pichia anomale Hansen (Kurzman), the blue-stain fungus Ceratocystis piceae (graphium) (Münch) Bakshi and a fungus closely similar to Ceratocystis araucariae (anamorph). T...
G Babuder,F Pohleven


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