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A rapid microscale assay for determining growth of wood inhabiting fungi against Lactobacillus metabolites
2009 - IRG/WP 09-20412
Lactobacillus sp. are well known for their ability to produce secondary metabolites with potent inhibitory activity against a wide range of bacterial species and there is increasing evidence to support their antifungal activity. These metabolites are often screened in laboratories using microscale 96-well plate optical density assays. The objective of this study was to develop an optical density a...
D O’Callahan, T Singh, I McDonald


Modelling of onset of mould growth for wood exposed to varying climate conditions
2009 - IRG/WP 09-20414
A performance-based service life design format based on climatic exposure on one hand and “resistance” of wood against mould growth on the other hand, is presented in this paper. A limit state for onset of mould growth is defined as the occurrence of traces of mould observed by microscopy. A dose-response model is proposed by which onset of mould growth can be predicted for an arbitrary climat...
S Thelandersson, T Isaksson, A Ekstrand-Tobin, P Johansson


Assessment of mould growth on coated wood - methods and application
2009 - IRG/WP 09-20423
Discolouring fungi reduce the service life of coated wooden claddings in façades and increase the total cost of ownership due to shorter maintenance intervals. The project “Enhanced service life on coated wooden facades” has as its main objective to develop new methods for early prediction of durability and longer aesthetic service life of coated wooden cladding related to consumer needs and ...
L Ross Gobakken, C M Whist, O Høibø, P J Hovde, E Larnøy


Analysis on dynamics of basidiomycetes in decayed wood by a method using non-specific amplification of DNA
2009 - IRG/WP 09-20426
A combination of non-specific amplification of DNA by Phi29 DNA polymerase and specific amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) by polymerase chain reaction (PCR) was applied to elucidate the ratio of basidiomycete species inhabiting decayed wood from an outdoor bench. After more than 8 hours incubation with Phi29 DNA polymerase, concentration of DNA ...
T Wada, K Igarashi, M Samejima


Modelling hyphal growth of the bio-incising fungus Physisporinus vitreus
2010 - IRG/WP 10-10710
The white-rot fungus Physisporinus vitreus preferentially degrades the pit membranes of bordered pits in tracheids and subsequently enhances wood permeability. Thus, P. vitreus can be used to improve the uptake of wood preservatives and environmentally-benign wood modification substances. This process can be used to enhance the use and sustainability of native conifer wood species by the wood indu...
M Fuhr, C Stührk, F W M R Schwarze, M Schubert, H J Herrmann


Postia placenta gene expression during growth in furfurylated wood
2010 - IRG/WP 10-10734
Modified wood can provide protection against a range of wood deteriorating organisms. But we still lack information about why the modified wood is protected from microbial attack. Several hypotheses have been put forward for the mode of action against wood decaying fungi, including inhibition of action of specific enzymes, but they still need further testing. In this study gene expression of the b...
G Alfredsen, C G Fossdal


Measurements of rot fungal activity as a function of moisture content by isothermal calorimetry
2010 - IRG/WP 10-20428
Measurements of heat production rate have been made on wood samples with the brown rot fungus Postia placenta at different moisture contents. The results clearly indicate that the heat production rate (a measure of respiration rate and activity) is moisture dependent. When the moisture content is decreased, less heat is produced, and when the moisture content is increased, more heat is produced. I...
L Wadsö, A Pilgård, G Alfredsen


Analyzing hyphal growth of the bio incising fungus Physisporinus vitreus with light-, confocal laser scanning- and, Synchrotron X-ray tomographic microscopy
2010 - IRG/WP 10-20438
Norway spruce wood is an important building material for the forest and wood industry. To improve wood permeability and uptake of wood modification substances Physisporinus vitreus is employed as a bioincising agent. In this study wood specimens were incubated with P. vitreus to assess hyphal colonisation and structural alterations of Norway spruce wood. After different incubation periods, semi-th...
C Stührk, M Fuhr, M Schubert, F W M R Schwarze


Onset of mould growth – the effect of climate variability and different geographic locations
2010 - IRG/WP 10-20446
This paper presents a study on how climate conditions at numerous locations in Sweden affect the risk of onset of mould on wood sheltered outdoors. The results indicate that the risk is clearly higher in the south compared to the north of Sweden. These differences are mainly explained by colder and dryer winter periods in the north which is unfavorable for development of mould. The results also sh...
M Häglund, T Isaksson, S Thelandersson


DNA-based tools for rapidly detecting, quantifying and monitoring ophiostomatoid fungi on beetles, in trees and wood products
2010 - IRG/WP 10-20450
Approximately half of the trees harvested for commercial purposes are lost because of native or introduced insects or insect-vectored microorganisms. Ophiostomatoid fungi, which are well adapted to dissemination by insects, include ~140 species of saprobes and pathogens. They are present worldwide, have high economical impact and many are subject to quarantine regulation. Thus, it is necessary to ...
L Khadempour, Young Woon Lim, S Massoumi Alamouti, C Breuil


Drying Rates and Mold Growth on Various Building Materials under Different Environmental Conditions
2010 - IRG/WP 10-20454
Mold growth on building materials is a major problem for homeowners. The most suitable method to control mold growth on building materials is to utilize design features, construction tools and practices that prevent moisture accumulation, and keep the wood as dry as possible. In order to achieve this, engineers and homebuilders have to know the effects of various temperature and moisture conditi...
Dian-Qing Yang


Mould growth on wood-based materials – a comparative study
2010 - IRG/WP 10-20455
Ten different wood-based materials - preservative-treated wood, fire retardant-treated wood, modified wood, WPCs and untreated references of pine sapwood and spruce - were tested for mould growth according to SP method 2899 during 42 days at 90% RH and 22°C. Even though the results must be interpreted carefully, they indicate significant differences in mould resistance between the materials tes...
P Johansson, J Jermer


Comparison of Bifenthrin Formulations for Their Skin and Eye Irritation Potential Using the Pollen Tube Growth Test
2010 - IRG/WP 10-30521
The Pollen Tube Growth Test (PTGT) is an effective screening method for measuring the potential of a chemical formulation to affect living cells. The PTGT has been compared against other types of testing, and it shows a good correlation with, for example, the Draize rabbit eye test and the human skin patch assay. It measures the EC50, which is the effective concentration at which 50% growth is o...
S Millward, P Lobb


Effects of Cunninghamia Lanceolata Heartwood Extracts on the Growth of Wood Decay Fungi
2010 - IRG/WP 10-30527
China-fir (Cunninghamia lanceolata) wood is well known for its good durability. This species is native to China and has been extensive planted there. Mainly, extractives are responsible for the wood resistance to decay fungi. In this paper, the extractives of China-fir heartwood were studied for their effects on various wood decay fungi. Sequential extraction of heartwood in hexane, ethyl acet...
Shujun Li, Jing Wang, Jian Li


Kinetic Water Vapour Sorption of Sitka Spruce at Variable Temperature Using the Parallel Exponential Kinetics Model
2010 - IRG/WP 10-40512
In this study the water vapour sorption of Sitka spruce (Picea sitchensis (Bongard) Carr.) was measured using a Dynamic Vapour Sorption (DVS) apparatus and then analysed using the parallel exponential kinetics (PEK) model. The water vapour sorption rate of wood quickly increased when the relative humidity was changed. With the elapse of time, the sorption rate gradually decreased within any specif...
Yanjun Xie, C Hill


Effects of global climate change on mould growth - Interactions of concern
2010 - IRG/WP 10-50270
An evident change in climate the last decades has been recorded, and combined effects of increased CO2, elevated temperature and altered precipitation regimes have been observed to represent a change to the fundamental drivers within ecosystems. Growth of moulds, both in nature and on man-made constructions and objects, will most likely increase due to changes in the climate. The survival, the rep...
L Ross Gobakken


The effects of acetylation level on the growth of Postia placenta
2011 - IRG/WP 11-10751
To understand the defence mechanisms utilized by decay fungi when exposed to different wood protection systems the study of gene expression can give us some answers. When the DNA sequences are known, primers can be designed to detect transcripts of genes with gene products related to basic cellular processes and hyphal growth. The characteristic gene products induced in different fungi by differen...
A Pilgård, G Alfredsen, C G Fossdal, C J Long II


Growth conditions for fungal decay in wooden constructions – practical experience versus literature
2011 - IRG/WP 11-10758
Data regarding moisture and temperature requirements of decay fungi found in literature have been compared to experience from observations in a large number of Norwegian buildings. A number of selected situations are discussed, among those exposure to high relative humidity, exposure to free water and decay in the supposedly adverse conditions on Svalbard. The moisture content in wood has to be at...
M Sand Sivertsen, J Mattsson


Methods of field data evaluation - time versus reliability
2011 - IRG/WP 11-20466
Field testing of wood and wood-based products is so far the most reliable way to get information on performance with respect to durability and expected service life. However, field trials are extremely time and cost consuming. The main objective of the present study was to investigate possible correlations between early stages of decay, preferably during the first 5 years, and the actual service l...
P Larsson Brelid, C Brischke, A O Rapp, M Hansson, M Westin, J Jermer, A Pilgård


In-ground variability of decay intensity within a test field
2012 - IRG/WP 12-20496
Timber exposed in the ground faces most severe conditions in terms of exposure to wetting and fungal decay in the terrestrial environment, therefore wood durability tests are often conducted in test fields preferably ensuring the occurrence of all relevant decay organisms. One can also expect differences in decay within an individual field-test site due to localized distribution of certain organis...
C Brischke, S Olberding, L Meyer, T Bornemann, C R Welzbacher


The effects of acetylation level on the growth of Postia placenta over 36 weeks
2012 - IRG/WP 12-40589
Genomic sequencing gives us a tool to systematically and rapidly discover novel genes, how their products function in the cell, and explore their interactions. When the DNA sequences are known, primers can be designed to detect transcripts of genes with gene products related to basic cellular processes and hyphal growth. The characteristic gene products induced in different fungi by different wood...
A Pilgård, G Alfredsen, C G Fossdal, C J Long II


Mould growth on wood-based materials – a simulated in-service study
2012 - IRG/WP 12-20503
Ten different wood-based materials including preservative-treated wood, fire retardant-treated wood, modified wood, WPCs and untreated references of pine sapwood and spruce were placed in three different environments (an attic and two crawl spaces) for a period of 26 months. Mould growth was analysed at five to seven month intervals in an effort to map the growth development. The relative humidit...
G Bok, P Johansson, J Jermer


Influence of a pulsed electric field on the growth of surface fungi
2013 - IRG/WP 13-10795
The colonization of mold and staining fungi are not only an aesthetical problem. Blue stain fungi are able to penetrate paint films. Maintenance of coatings on wood are time consuming and costly. A new approach uses a pulsed electric field (PLEOT) in order to protect wood against surface fungi. This technology is evaluated in non-sterile laboratory conditions on different coated and uncoated Scots...
M Starck, A Treu


Detection of Aureobasidium as the dominant fungus on coated wood
2013 - IRG/WP 13-10796
Wood is often covered with a coating in order to protect the surface. Unwanted dark mold growth on paint covered surfaces in external conditions is a familiar phenomenon. Simple microbial techniques have commonly been used to determine the dominant fungal species present in the discoloured area, for example morphological analysis of microscopic preparations of fungal pieces sticking to an adhesive...
E J Van Nieuwenhuijzen, M F Sailer, R A Samson, O C G Adan


Inhibitory extractives or nutrients? Mould growth on coated heartwood and sapwood of Pinus sylvestris and Gleditsia triacanthos
2013 - IRG/WP 13-10806
Sometimes mould fungi grow over weathered coated sapwood, whereas the coated heartwood is not colonised. Two factors may be responsible for these differences: Either the larger content of nutrients in the sapwood, or the content of biocidal extractives in the heartwood. In this study the factors for the difference in mould growth on coated heartwood and sapwood of Pinus sylvestris (Scots Pine) and...
D Lukowsky, N Buddruhs


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