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The true dry rot fungus (Serpula lacrymans) as a wound parasite of living Norway spruce (Picea abies) in the Czech Republic
2001 - IRG/WP 01-10389
Some late 19th century Central European mycologist reported finds of the true dry rot fungus (Serpula lacrymans) from nature. They even suggested that it could be a parasite of living trees. However examination of herbarium material has shown that the species they found was thin-fleshed dry rot (Serpula himantioides) rather than Serpula lacrymans. In 1992 the Czech mycologist Kotlaba reported find...
J Bech-Andersen, S A Elborne, J Andreasson, J Ch Sterler


Oxalic acid metabolism of Postia placenta
1992 - IRG/WP 92-1566
The oxalic acid metabolism of Postia placenta was studied in liquid culture in order to identify the metabolic pathways of biosynthesis and to determine whether oxalic acid physiology could be correlated with efficiency in wood decay. Oxalic acid production was determined for test strain MAD698 grown in a basal defined medium with a variety of different carbohydrate and nitrogen sources. The highe...
J A Micales


Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips, immunolabeled with or without prior fixation, and examined by SEM. Enzymes were localized on the hyphal surface and on the clumped fibrillar ...
F Green III, C A Clausen, M J Larsen, T L Highley


The effect of Tween 80 on the growth, morphology, and enzyme secretion of Postia placenta
1990 - IRG/WP 1456
The nonionic surfactant Tween 80 (polyethylene oxide sorbitan mono-oleate) has been reported to increase enzyme production and/or secretion in bacteria and fungi. Such a procedure could greatly facilitate research into the physiology of wood-decay fungi since quantities of available enzyme are often limiting. The brown-rot fungus Postia placenta was grown in a synthetic medium supplemented with 0,...
J A Micales


Changes in some important mechanical and physical properties of untreated and treated mining timber after exposure to the conditions of a colliery
1996 - IRG/WP 96-20082
Each of salt treated and untreated mining timber (wedge) pairs with 5x15 cm² cross-section and 155 cm length prepared from Scots pine, Bornmüller's fir and oriental beech was cut in the small standard test specimens to determine changes in some important mechanical and physical properties, 13 month after exposure to the conditions of Kozlu Hard Coal Mines in the depth of 52 m. Thus, the...
A Bobat


Serpula lacrymans the dry rot fungus. Revue on previous papers
1989 - IRG/WP 1393
It is found that the Dry rot fungus Serpula lacrymans grows in houses only because of its need for basic materials to neutralize the oxalic acid production or heavy metals which celate the oxalic acid. The average distance from the mycelium to the basic materials is found in average to be 14.2 cm with a variation from 0-100 cm. In contrast to Serpula lacrymans the Coniophora puteana and the Rigido...
J Bech-Andersen


Production of monoclonal antibodies to Serpula lacrymans and their application in immunodetection systems
1993 - IRG/WP 93-10004
Monoclonal antibodies were produced against both whole cell mycelial extracts and exo-antigen extracts of the causative organism of dry rot, Serpula lacrymans. The antibodies were tested for cross-reactivity against fifteen strains of Serpula lacrymans and nine other fungal species. These species represented either other wood decay fungi or non-decay fungi commonly co-isolated with Serpula lacryma...
H Glancy, J W Palfreyman


Screening of fungal strains for wood extractive degradation
1998 - IRG/WP 98-10254
Fungal strains were screened for their ability to degrade apolar extractives in wood from scots pine. The degradation of total wood extractives by 91 different strains was monitored in stationary batch assays incubated for 6 weeks. The results obtained show that the ability of wood-inhabiting fungi to utilize wood extractives varied greatly, even for different isolates of the same species. Fungal ...
J Dorado, M J Martinez-Inigo, T A van Beek, F W Claassen, J B P A Wijnberg, R Sierra-Alvarez


Preliminary investigation on the natural durability of Guayule (Parthenium argentatum)-based wood products
2000 - IRG/WP 00-40154
Conventional preservatives used to protect wood from insect and microbial damages are presently of major concern to human health and the environment. Finding alternative and economical preservatives has not been successful. Previous studies have shown that the resinous material extracted from the guayule plant (Parthenium argentatum, Gray) has both insect- and microbial-resistant properties. Unfor...
F S Nakayama, P Chow, D S Bajwa, J A Youngquist, J H Muehl, A M Krzysik


Effect of remediation on the release of copper, chromium, and arsenic from particleboard made from CCA treated wood
2001 - IRG/WP 01-50170
This study sought to determine the effect of remediation with oxalic acid (OA) extraction and Bacillus licheniformis fermentation on the release of copper, chromium, and arsenic from particleboard made from remediated wood particles and also investigates durability of the particleboard against white and brown- rot fungi. Particleboard samples were manufactured using untreated, CCA-treated, OA-extr...
S N Kartal, C A Clausen


Free radical process controlled by manganese peroxidase and lipid-related metabolites produced by Ceriporiopsis subvermispora
2001 - IRG/WP 01-10412
Ceriporiopsis subvermispora, a specific lignin-degrading fungus produced free unsaturated fatty acids (USFAs) including 9,12-octadecaienoic asid, together with saturated fatty acids (SFAs) at an incipient stage of cultivation on wood meal cultures. In prolonged cultivation period after two weeks, the amount of intact fatty acids decreased with increasing in organic hydroperoxide and TBARS producti...
T Watanabe, M Enoki, S Sato, Y Honda, M Kuwahara, N Shirai, K Messner


Neem leaves, a potential source for protection of hardwood against wood decaying fungus
2005 - IRG/WP 05-30370
Neem leaves are widely recognized for their insecticidal, pesticidal, fungistatic and fungitoxic ability. The present study is to investigate the efficacy of neem leaves extractives against wood destroying microbes, a brown and a white rot. Neem leaves extractives prepared in various solvents such as petroleum ether, acetone, ethanol, methanol and hot water were tested at a concentration levels of...
S Dhyani, S Tripathi, V K Jain


Degradation of resin constituents in various wood species by the white rot fungus Bjerkandera sp. strain BOS55
1999 - IRG/WP 99-10301
In previous studies, the white-rot fungus Bjerkandera sp. strain BOS55 was shown to cause extensive degradation of lipophilic extractives (resin) in Scots pine wood. Further research was carried out in order to investigate the ability of Bjerkandera sp. for reducing resinous constituents in various softwood (Douglas fir, larch and spruce) and hardwood species (birch, beech and poplar). The greates...
J Dorado, T A Van Beek, F W Claassen, R Sierra-Alvarez


The action of siderophores isolated from Gloeophyllum trabeum on the structure and crystallinity of cellulose compounds
1991 - IRG/WP 1479
Low molecular weight, high affinity iron-binding compounds (siderophores) were isolated from the brown-rot fungus Gloeophyllum trabeum. The compounds were shown to be inducible by iron starvation and could be purified by ultra-filtration, ethyl acetate extraction, column chromatography and preparative HPLC. The isolated compounds were shown by analytical and immunological techniques to be produced...
J Jellison, V Chandhoke, B Goodell, F Fekete, N Hayashi, M Ishihara, K Yamamoto


A model for attack at a distance from the hyphae based on studies with the brown rot Coniophora puteana
1995 - IRG/WP 95-10104
In timber infested by brown rot fungi, a rapid loss in strength is attributed to production of hydroxyl radicals (HO·) at a distance from the hyphae. The immediate precursor is Fenton's reagent (Fe(II)/H2O2), but the pathways leading to Fe(II) and H2O2 have remained unclear. Cellobiose dehydrogenase, purified from cultures of Coniophora puteana, will couple oxidation of cellodextrins to ...
S M Hyde, P M Wood


Extracellular carbohydrate production by isolates of Postia (=Poria) placenta
1989 - IRG/WP 1388
A monokaryotic strain of Postia (=Poria) placenta, ME20, which is unable to degrade wood, also failed to produce extracellular polysaccharide when grown in liquid culture, regardless of carbon source or concentration. Other isolates of Postia placenta, including another monokaryon and a hybrid of this monokaryon with ME20, produced large quantities of this material. The polysaccharide consisted pr...
J A Micales, A L Richter, T L Highley


Moisture Adsorption Behaviour of Rubber Wood Decayed by Brown and White rot Fungi
2005 - IRG/WP 05-40306
Moisture adsorption behaviour of rubber wood blocks decayed by each brown rot and white rot fungi was studied. The adsorption isotherms of decayed wood were compared with the isotherm of undecayed wood. The wood decayed by brown rot adsorbed less moisture as compared to sound or undecayed wood at all humidity levels. No significant difference was observed in adsorption behaviour of white rot dec...
S S Chauhan, H C Nagaveni


Mycoparasitism by some white rot fungi on blue stain fungi in culture
1986 - IRG/WP 1304
When studying biological control of blue stain an interesting phenomenon of parasitism by some white rot fungi on blue stain fungi has been encountered. The majority of the 22 tested white rot fungi act parasitically upon blue stain fungi. The most interesting species among them is Bjerkandera adusta (Willd. ex Fr.) Karst., causing almost complete or complete decoloration of the hyphae of the blue...
R Benko, B Henningsson


Pretreatment decay in poles of Pinus sylvestris
1987 - IRG/WP 1329
Storage damage by blue stain and decay in poles before impregnation has been increasingly observed during recent years and there have been reports that salt-impregnated poles today are of lower quality because of incipient decay being increasingly common. The attacks may have started at the time of cutting and then continued during the period of seasoning at the plant, which could be as long as tw...
H Lundström, M-L Edlund


Why don't more people work with Serpula lacrymans nowadays? A discussion of some of the different approaches to experimenting with this unique fungus
1989 - IRG/WP 1383
Remarkably few laboratories are currently investigating this well-known fungus. Not only has it failed to gain acceptance as a standard test organism (for wood preservative evaluation) in many countries, but is also sometimes excluded from pure research studies when it ought to be an obvious first choice as a representative basidiomycete. This paper discusses some different approaches that have be...
J D Thornton


Antagonism of Gliocladium virens against wood decay fungi
1995 - IRG/WP 95-10102
Antagonistic abilities of a commercial biofungicide, Gliocladium virens (GL-21, Grace) were evaluated against three white-rot fungi, Trametes versicolor, Phlebia brevispora, Irpex lacteus, and three brown-rot fungi, Postia placenta, Neolentinus lepideus, Gloeophyllum trabeum. In dual culture of Gliocladium virens and wood decay fungi, Gliocladium virens rapidly overgrow the decay fungi and killed ...
T L Highley, L Ferge


Antagonistic effects of Dacrymyces stillatus against growth of other fungi
1991 - IRG/WP 2363
The antagonistic effects in dual cultures on malt extract agar of 5 wood decay fungi and the antagonistic effects of Dacrymyces stillatus against 1 mould fungus and 1 blue stain fungus were determined. Dacrymyces stillatus was the only fungus which exhibited clear antagonistic effects. The effects of Dacrymyces stillatus against the different test fungi varied however to a great extent. Dacrymyces...
J Bjurman


Detection of the dry rot fungus Serpula lacrymans by amplified ribosomal DNA restriction analysis
1998 - IRG/WP 98-10245
Isolates of the dry rot fungus Serpula lacrymans and the wild merulius S. himantioides were investigated by amplified ribosomal DNA restriction analyses (ARDRA) of the internal transcribed spacers (ITS). The technique uses the polymerase chain reaction (PCR) to amplify the variable region of the ITS between the conserved 18S and 28S genes of the nuclear ribosomal DNA (rDNA). The ITS region of all isolates of Serpula lacrymans and S. himantioides was successfully amplified. The length of the amplified product was about 630 bp. Subsequent double digest of the ITS with the restriction endonucleases Haelli and Taql separated the closely related fungi by species-specific fragments. Thus, ARDRA-ITS proved to be suited for the detection of the dry rot fungus.
O Schmidt, U Moreth


Upgrading the fungal resistance of OSB
1999 - IRG/WP 99-40138
There is a perception that oriented strand board (OSB) is less resistant to fungl than plywood under conditions of moderate exposure to moisture. Douglas fir-faced plywood (DFP) has been suggested as a benchmark for acceptable durability under such conditions. This project was initiated to determine the minimum level of low-toxicity chemical treatment needed to upgrade the fungal resistance of OSB...
P I Morris, J E Clark, D Minchin, R Wellwood


Evaluation of hydroxamates as wood preservatives
2004 - IRG/WP 04-30330
Fungicidal activity of different hydroxamates was investigated by laboratory malt-agar block tests to evaluate their potential as wood preservatives. Results obtained indicate that benzohydroxamic acids substituted or not on the aromatic nuclei were unable to protect wood blocks against the white rot fungus Coriolus versicolor while 2-hydroxypyridine-N-oxide (2-HPNO) possesses an interesting activ...
P Gérardin, S Dumarçay, E Gelhaye, A Mbamba, V Mussavu


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