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Detection of the dry rot fungus Serpula lacrymans by amplified ribosomal DNA restriction analysis
1998 - IRG/WP 98-10245
Isolates of the dry rot fungus Serpula lacrymans and the wild merulius S. himantioides were investigated by amplified ribosomal DNA restriction analyses (ARDRA) of the internal transcribed spacers (ITS). The technique uses the polymerase chain reaction (PCR) to amplify the variable region of the ITS between the conserved 18S and 28S genes of the nuclear ribosomal DNA (rDNA). The ITS region of all isolates of Serpula lacrymans and S. himantioides was successfully amplified. The length of the amplified product was about 630 bp. Subsequent double digest of the ITS with the restriction endonucleases Haelli and Taql separated the closely related fungi by species-specific fragments. Thus, ARDRA-ITS proved to be suited for the detection of the dry rot fungus.
O Schmidt, U Moreth


Sub-group on Basidiomycete tests: Experimental programme and schedule of participants
1980 - IRG/WP 2145
The objectives of this IRG COLLABORATIVE BASIDIOMYCETE TEST are to compare results and assess reproducibility of a miniaturised wood block toxicity test when undertaken by different workers in different laboratories. The co-operative scientists and institutes are: Dr K Messner from Vienna (Austria); Dr R S Smith from Forintek (Canada); Mr J Hansen from Sadolins (Denmark); Mr B Jensen from Gori (De...
A F Bravery


Upgrading the fungal resistance of OSB
1999 - IRG/WP 99-40138
There is a perception that oriented strand board (OSB) is less resistant to fungl than plywood under conditions of moderate exposure to moisture. Douglas fir-faced plywood (DFP) has been suggested as a benchmark for acceptable durability under such conditions. This project was initiated to determine the minimum level of low-toxicity chemical treatment needed to upgrade the fungal resistance of OSB...
P I Morris, J E Clark, D Minchin, R Wellwood


Evaluation of hydroxamates as wood preservatives
2004 - IRG/WP 04-30330
Fungicidal activity of different hydroxamates was investigated by laboratory malt-agar block tests to evaluate their potential as wood preservatives. Results obtained indicate that benzohydroxamic acids substituted or not on the aromatic nuclei were unable to protect wood blocks against the white rot fungus Coriolus versicolor while 2-hydroxypyridine-N-oxide (2-HPNO) possesses an interesting activ...
P Gérardin, S Dumarçay, E Gelhaye, A Mbamba, V Mussavu


Resistance of board materials against fungal decay: A comparative experiment on Kolle flask tests method and direct test method
1985 - IRG/WP 2243
Two sets of plywood (Aucoumea klaineana Pierre) 7 and 11 ply, treated by two preservatives, and untreated, were tested by two methods: 1. Kolle flask test method on malt-agar substrate according to the French standard NF B 51.295; 2. A direct test method in non sterile conditions according to DocNo: IRG/WP/2214. The exposure time far both methods was 12 weeks. After exposure, the degree of attack ...
G R Y Déon, L N Trong


A novel technique for comparative toxicity studies of potential insecticidal wood preservatives
1983 - IRG/WP 2198
For some years now a device has been under development at the New Zealand Forest Research Institute, the purpose of which is to study the effects of sub-lethal amounts of stomach poisons (but also of fumigants and contact active materials) on the co-ordinative abilities of a cerambycid larva. This insect is Prionoplus reticularis - indigenous to New Zealand its larvae feed in decaying logs and are...
D J Cross


Questionnaire relating to the occurrence, prevention and repair of Dry Rot attacks in buildings
1989 - IRG/WP 1418
Dry Rot attack in buildings has become an increasing problem in Denmark. The reason is that old multi-storey houses from about 1890-1920 are being renovated and modernised and connected to this well-hidden Dry Rot attacks are detected. The traditional methods for repair used in Denmark are very expensive but with a high degree of confidence when done carefully. There is a big need for development ...
A P Koch


Extracellular laccase production in brown-rot fungus Coniophora puteana
2001 - IRG/WP 01-10391
Coniophora puteana, one of representing brown-rot fungus, was investigated in terms of extracellular ligninolytic enzyme production. Three fungal strains of C. puteana were inoculated in liquid culture medium with sawdust of pine and oak wood. Extracellular laccase production was observed in some strains of C. puteana in the culture medium with sawdust of oak wood. The highest activity occurred at...
Kwang-Ho Lee, Seung-Gon Wi, Yoon Soo Kim


Antagonism of Scytalidium lignicola against wood decay fungi
1989 - IRG/WP 1392
Antagonistic abilities of Scytalidium lignicola against white- and brown-rot wood decay fungi were evaluated. Scytalidium lignicola did not produce inhibition zones but overgrew the decay fungi on a malt-agar medium and in most cases killed them. Pretreatment of Douglas-fir and Southern pine blocks with Scytalidium lignicola prevented decay. Blocks that were heated or treated with propylene oxide ...
T L Highley


The critical timing of inspections for field testing of natural durability
1994 - IRG/WP 94-20018
An overestimation of performance will almost always result if the condition of a specimen is considered to be attained at the precise time that an inspection is carried out. The time at which the condition is actually attained, referred to in this document as the "Condition time", is best considered to be halfway between two successive inspections. The requirement to obtain data that is relevant t...
J D Thornton


Production of monoclonal antibodies to fungal metabolites
1986 - IRG/WP 1306
The role of fungal extracellular enzymes in wood biodegradation is incompletely understood. Our lab is beginning a project utilizing monoclonal antibodies to characterize extracellular metabolites of the brown rot fungus Poria placenta Fr. (Cooke). Monoclonal antibody technology takes advantage of the ability of antibody secreting spleen cells from immunized mice to fuse in the presence of polyeth...
J Jellison, B Goodell


Leachability of active ingredients from some CCA treated and creosoted poles in service. A progress report after 10 years testing
1990 - IRG/WP 3627
CCA K33 TYPE B treated or creosoted poles, 10 pieces of each treatment type, have been monitored from the treatment plant to an electricity line, which was build up in Southern Finland in 1978. Preservative retention was determined by taking borings at four different levels: 1.5 m from the top end, 1 m above ground line, ground line and 0.5 m below ground. Determinations were made before setting u...
A J Nurmi


Immunolabelling studies on the detection of enzymes during the degradation of wood by Phanerochaete chrysosporium
1988 - IRG/WP 1364
The degradation of lignin in native lignocellulosic substrates by white rot fungi is poorly understood. Biochemical studies have shown the involvement of a number extracellular ligninolytic enzymes released by white rot fungi which are capable of the oxidative conversion of DHP's (lignin model compounds) in vitro, but to date conclusive evidence for occurrence of these enzymes in wood und...
G F Daniel, T Nilsson, B Pettersson


Effects of heart wood extractives in Dahoma (Piptadeniastrum africanum) on decay resistance to white- and brown-rot fungi
2004 - IRG/WP 04-10536
Dahoma (Piptadeniastrum africanum) is a durable but a lesser utilised tropical hardwood species from Ghana that has the potential of being used as an alternative to Iroko (Milicia excelsa) for many end uses. However, the timber gives off a strong odour especially when wet and during processing. The high durability and the strong odour of the timber could be due to the presence of extractives in th...
Zeen Huang, K Maher, S A Amartey


Bioefficacy of Cunapsol® treated western cedar and southern yellow pine
1996 - IRG/WP 96-30120
Western red cedar and southern yellow pine sapwood were dip treated with a new formulation of waterborne copper naphthenate (Cunapsol®) which complies with VOC regulations. Cunapsol dip treated samples were oven dried at 50°C for 48 hours and then water leached for two weeks. Decay resistance and leachability of preservatives from dip treated specimens were evaluated according to AWPA (American ...
D P Kamdem, M H Freeman, T L Woods


The identification of the carbohydrate degrading enzymes from the crude extract of brown-rot fungus Gloeophyllum trabeum
1991 - IRG/WP 1483
The brown-rot fungus, Gloeophyllum trabeum, produces a pattern of carbohydrate degrading enzymes during the wood decay. In liquid sawdust media the activities of endo-b-1,4-gluganase and endo-b-1,4-xylanase were at the maximum after 5-6 weeks cultivation. The production of enzymes started immediately after inoculation suggesting that the degradation of hemicellulose and easily degradable parts of ...
A-C Ritschkoff, J Buchert, L Viikari


Comparative study of lignocellulolytic activities of Pleurotus spp. and white rot and brown-rot fungi
1997 - IRG/WP 97-10209
Field and fungal cellar trials have been set up to assess the biocontrol potential of a selected Trichoderma viride isolate in a situation representative of the end use of treated timber in ground contact situations. These trials are designed to give information about the efficacy of biological control as well as the suitability of existing chemical treatment methods for use with biocontrol fungi....
H L Brown, A Bruce


Inhibition of the biodegradation of coniferous and broadleaved wood by new imidazolium salts
2003 - IRG/WP 03-30320
Studies were carried out on fungicidal values of fourteen potential wood preservatives – modified quaternary heteroaromatic compounds using the screening agar-block. The vacuum treated blocks (22mm x 17mm x 12mm) of Scots pine (Pinus sylvestris L.), spruce (Picea excelsa L.), beech (Fagus silvatica L.) and birch (Betula verrucosa Ehrh.) with testing compounds were exposed to Coniophota puteana a...
J Zabielska-Matejuk, J Pernak, W Wieczorek


Implications for comparability of laboratory experiments revealed in studies on the effects of population density on the vigour in groups of Coptotermes lacteus (Froggatt) and Nasutitermes exitiosus(Hill) (Isoptera: Rhinotermitidae, Termitidae)
1983 - IRG/WP 1197
(Summary of paper 1194) The vigour - survival and wood consumption - of groups of Coptotermes lacteus and Nasutitermes exitiosus was measured when termites were kept at different population densities (g termites/ mL) by changing group size and/or volume of the holding container. A characteristic pattern emerged. At low population densities (< 0,01 g/mL) performance of termites improved with an increase in group size; at higher population densities it tended to decline. The impact of altering group size and container volume on termite vigour was most pronounced at low densities; at higher population densities, performance tended to be more stable but declined markedly when termites became overcrowded. In most jar-type experiments on termites, especially those conducted in Europe and the U.S.A., small groups of termites are housed in disproportionately large jars, resulting in very low, sub-optimal population densities. Suggestions are made for improvements in experimental design that would lead to an enhancement of the comparability of results from different laboratories.
M Lenz, R A Barrett, E R Williams


Detection of termite attack in wood using acoustic emission
1989 - IRG/WP 2331
Acoustic emission (AE) is the elastic wave produced by the strain energy released in the process of fracture of a material and propagates through it. The object of this report is to detect AEs produced by the termite activities and to evaluate the possibility of using an AE monitoring test to nondestructively detect the termite attack in wood. It was revealed that AEs were detected from the specim...
Y Fujii, M Noguchi, Y Imamura, M Tokoro


Some Experiences with Stake Tests at BAM Test Fields and in the BAM Fungus Cellar Part 1: Comparison of Results of Visual Assessments and Determinations of Static Moduli of Elasticity (MOE)
2005 - IRG/WP 05-20319
With examples of routine in-ground stake tests differences are shown in the performance of wood preservatives at the BAM test fields Lehre and Horstwalde and in the BAM fungus cellar. Signs of attack of micro-organisms were assessed visually according to EN 252. Periodical determinations of static moduli of elasticity (MOE) revealed the influence of the attack on the elastic properties of the wood...
M Grinda, S Göller


Blue stain in timber in service. Progress review of Sub-group collaborative tests - 1981/82
1982 - IRG/WP 2178
Following from the discussions after reporting Document IRG/WP/2146 at the Meeting in Sarajevo, 1981, it was decided that 1.) more precise definition was needed of the artificial weathering schedules used in the work - participants to re-check Table 8 and send further details or amendments to Dr Bravery; 2.) a further test employing a wider range of chemicals was necessary using a smaller range of...
A F Bravery, D J Dickinson


Hole delimination inside round timber via ultrasonic techniques
1990 - IRG/WP 2358
A fast and economic circular scanning method to localize and evaluate holes inside round timber is presented. This method consists in measuring transit times of the ultrasonic wave by fixing the transmitter probe in the perimeter of the sample, then moving the receiver probe every ten degrees until completion of the circle. The scanning is done with a cheap and light instrument. Tests are made wit...
G Prieto, A Fernández Cancio


Variation in field test performance of untreated and CCA-treated lesser-known Surinamese wood species
2000 - IRG/WP 00-20213
In Suriname three different field test sites have been used to verify the biological durability of CCA-treated wood and a range of wood species including lesser-known and lesser-used wood species. In total 37 tropical wood species were tested untreated and for 17 of the less durable species CCA-treatments were included as well. Although the three sites are located in the tropical region they diffe...
J Van Acker, M Stevens, L Comvalius


A physiological role of the glyoxylate and TCA cycles in fruitbody formation of the coppertolerant brown-rot fungus Fomitopsis palustris
2002 - IRG/WP 02-10430
Changes in activity of the representative enzymes involved in biosynthesis of oxalic acid (oxalate) and carbon metabolism of glucose were investigated in relation to the fruit body formation of the copper-tolerant brown-rot fungus Fomitopsis palustris. Changes in specific activities of the two glyoxylate (GLOX) cycle key enzymes (isocitrate lyase (ICL) and malate synthase (MS)), the two oxalate-fo...
Jeong-Jun Yoon, T Hattori, M Shimada


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