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Wood decay fungi from New Zealand ‘leaky’ buildings: PCR identification and laboratory decay tests of wood preservative-treated Pinus radiata (Part 1)
2007 - IRG/WP 07-10620
Fungi colonising Pinus radiata D. Don framing timber of ‘leaky’ New Zealand buildings were isolated to produce pure cultures. Mycelia from these cultures on agar media were collected to extract DNA. To identify the fungi to the species level, polymerase chain reaction (PCR) with primer pairs ITS1-F and ITS4 were performed followed by sequencing of the internal transcribed spacer (ITS) region. ...
D Stahlhut, R L Farrell, R Wakeling, M Hedley


Detecting fungal DNA in treated and non-treated wood
2007 - IRG/WP 07-10621
Isolating fungi from wood has long involved culturing on selective media followed by identification using various keys. This process can be cumbersome, costly, and, most importantly, not always capable of detecting all of the fungi present. The recent development of molecular methods for isolation and identification of fungi has created tremendous opportunities for expanding our knowledge of the...
C Freitag, M Freitag, J Morrell


The use of X-ray diffraction for analyzing biomodification of crystalline cellulose by wood decay fungi
2007 - IRG/WP 07-10622
X-ray diffraction (XRD) is based on the creation of an interference pattern by x-rays when they encounter a regularly spaced matrix. In wood, this process has been used to determine, among other things, the average width of the cellulose microcrystals, the percent of crystalline cellulose within the wood, and can be used to examine the changes in these parameters during degradation. Enhanced under...
C Howell, A C Steenkjær Hastrup, J Jellison


Gypsum effects on ‘dry rot’ wood degradation as a function of environment
2007 - IRG/WP 07-10624
‘Dry rot’ fungi are a unique group of brown rot fungi that can degrade wood away from ground contact where other fungi fail to colonize. Successfully occupying this niche is partially due to efficient water and nutrient transport, but mobilizing elements, notably calcium (Ca) and iron (Fe), from adjacent building materials has also been implicated in their success. Here we report a series of t...
J Schilling, J Jellison


Effect of chitosan on the morphology and ultrastructure of two wood inhabiting fungi
2007 - IRG/WP 07-10606
An investigation was undertaken to compare the antifungal effects caused by chitosan against two selected wood degrading fungi Sphaeropsis sapinea and Trichoderma harzianum on their growth, morphological and ultra-structural characteristics, using differential interference microscopy, epifluorescence microscopy and transmission electron microscopy. The effect of chitosan on fungal morphology was ...
T Singh, A P Singh, G Daniel


Growth and succession of mould on commercial paint systems in two field sites
2007 - IRG/WP 07-30421
Discolouring fungi reduce the service life of coated wooden claddings in façades and increase the total cost of ownership due to shorter maintenance intervals. It is of major importance to find paint systems for wood which are durable and have a high resistance to mould growth. A total of 15 paint systems from 9 different manufacturers were exposed on two field sites, Sørkedalen and Birkenes, ...
L Ross Gobakken, K M Jenssen


Study of natural durability of Spanish Eucalyptus globulus wood
2007 - IRG/WP 07-10617
In some countries such as Australia, the wood of Eucalyptus globulus is recommended, due to its high durability, in risk situations where attack by organisms is likely to occur. In Galicia this species has been used for many decades for timber structures, which are still used. However, European Standard EN-350-2 places Eucalyptus globulus in the worst category of natural durability, a situation th...
D Lorenzo, M T Troya, M J Prieto, C Baso, M Touza


Potentiality of use extracts from Tetraclinis articulata like biocide against wood destroying organisms: Reticulitermes santonensis
2007 - IRG/WP 07-30418
Screening trials were conducted to determine the antitermitic and antifungal properties of essential oil and methanolic extractives obtained from Tetraclinis articulata heartwood. Extract-treated cellulose pads were used to evaluate antitermitic activity and complete termite mortality was obtained with the essential oil: the threshold is situated under 5% (v/v). Standardised tests according Europe...
F El hanbali, N Amusant, F Mellouki, M Akssira, C Baudasse


Effects of caffeine on growth of wood-decaying fungi
2007 - IRG/WP 07-30427
The wide-used triazoles alter the biosynthesis of ergosterol leading to a disruption of fungal membrane functions. The aim of this study was to investigate the effects of another cell wall perturbing agent, caffeine, in combination with propiconazole on the growth of wood-decaying fungi. 10 mM caffeine inhibits totally the growth of five fungal species. The obtained results showed that caffeine ha...
S Lekounougou, J P Ondo, J P Jacquot, G Nevers, P Gérardin, E Gelhaye


Laboratory test of blue stain control by chemicals
2007 - IRG/WP 07-30430
8 chemicals and some mixtures against Botryodiplodia theobromae Pat., Fusarium verticillioides(Sacc.)Nirenberg, Trichoderma harzianum Rifai, Trichoderma viride Pers., and Penicillium purpurogenum Stoll were conducted in laboratory by inhibition zone in the paper. The results were shown that: CBZ and benomyl as well as copper oxine alone are much high efficacy for inhibiting most of the 5 fungi tha...
Zhijuan Wang, Zehui Jiang, Mingliang Jiang, Chungen Piao


The influence of pentachlorophenol on mycelial growth of wood decay fungi Trametes versicolor, Grifola frondosa, Hypoxylon fragiforme, and Coniophora puteana
2007 - IRG/WP 07-30437
Polychlorinated phenols have been one of the most commonly used biocides. In recent time they have been increasingly eliminated from the marked. Because of their high stability, they are still present in numerous products, including impregnated wood. In our research we studied in vitro the influences of pentachlorophenol and/or sodium pentachlorophenolate on mycelial growth of four wood degrading ...
F Pohleven, B Boh


Effect of ozone treatment on survival of termites and wood decay fungi
2007 - IRG/WP 07-40365
There is increased concern about the potential for transport of invasive pests on or in untreated wood. Current regulations utilize heating or fumigation as mitigation tools, but both have drawbacks that have stimulated interest in new phytosanitation methods. Ozone fumigation has been shown to control insects, fungi and bacteria on a variety of agricultural products. Systems for the controlled g...
A M Taylor, L J Mason, J J Morrell


Genome-wide survey of cellulase related genes of white rot fungus,Pleurotus ostreatus
2007 - IRG/WP 07-10627
A white rot fungus, Pleurotus ostreatus, which is a important edible mushroom, has received much attention to apply to bioremediation and bioconversion because it has both cellulase and ligninase. To confirm copy number of cellulase-related genes encoded in P. ostreatus genome, we attempted to genomic Southern hybridization of P. ostreatus. The draft genome sequence and a large quantity of EST and...
T Tamenori, S Horisawa


Antifungal activity and synergistic effect of cinnamaldehyde combined with antioxidants against wood decay fungi
2007 - IRG/WP 07-30445
The objective of this study was to investigate the antifungal activity and synergistic effect of cinnamaldehyde combined with antioxidants against wood decay fungi. Five antioxidants, propyl gallate, octyl gallate, quercetin, eugenol and catechin were tested against various wood decay fungi. Octyl gallate and eugenol were found to be the only two antioxidants processed antifungal activities. IC50 ...
Fu-Lan Hsu, Tsair-Bor Yen, Hui- Ting Chang, Shang-Tzen Chang


Effect of cinnamon oil and clove oil against major fungi identified from surface of rubberwood (Hevea brasiliensis)
2007 - IRG/WP 07-30446
Antifungal activities of cinnamon oil and clove oil at ratio 1:1 and pure against major fungi found on surface of rubberwood (Aspergillus niger, Penicillium chrysogenum, and Penicillium sp.) were investigated using the broth dilution method. The minimum inhibitory concentration (MIC) of the combined cinnamon and clove oils for these test moulds was determined to be 80 µL/mL which were less than o...
Narumol Matan, Nirundorn Matan


An Investigation on Use and Durability of Some Industrial and Domestic Woods of Iran against Destructive Factors in Caspian Sea
2008 - IRG/WP 08-10636
In this study the use of domestic and commercial woods of the Caspian Sea forest fringes and evaluation of their durability against marine destructive factors were investigated. The samples of elm (Zelkova carpinifolia), oak (Quercus castanifolia), beech (Fagus orientalis), Maple (Acer insgin), Alder (Alnus subcordata), and horn beech (Carpinus betulus) species were impregnated with CCA (Chrome–...
S M Kazemi


Biodeterioration of cultural monuments in the Republic of Macedonia
2008 - IRG/WP 08-10640
Research on the biodeterioration of the cultural heritage in the Republic of Macedonia was conducted from 2004 to 2006, one month per year. The expertise was focused on the biodeterioration caused by wood-inhabiting fungi and moulds. A total of 38 cultural monuments, including 37 monasteries and churches and one fortress, were inspected in the areas of the towns of Skopje, Ohrid, Demir Hisar, Deba...
I Irbe, M Karadelev, I Andersone, B Andersons


Decay Resistance Properties of Hemicellulose-Extracted Oriented Strand Board
2008 - IRG/WP 08-10644
There is increasing interest in using cellulosic biomass as the basis for a renewable source of energy. Although the traditional focus has been on cellulose, researchers are also exploring the possibility of using hemicelluloses because they can be removed with hot water leaving the wood product intact for other applications. However, there is concern that removing hemicelluloses from the precur...
C Howell, J Paredes, S Shaler, J Jellison


Performance of softwood preservative treated stakes after 4 years exposure in-ground to decay fungi and termites in tropical Australia
2008 - IRG/WP 08-10643
This field study was based on the 1993 IUFRO method and designed as a graveyard (in-ground) test in the tropical region of the Northern Territory to examine the efficacy of novel preservative formulations developed as alternative protection for softwood timber against decay fungi and termites for Hazard Class 3 and 4. This study was supported by the Forest and Wood Products Research and Develop...
B M Ahmed (Shiday), J R J French, S R Przewloka, P Vinden, J A Hann, P Blackwell


Evaluation of natural durability of solid wood and mixed heartwood-sapwood Cypress (Cupressus sempervirens L.) plywood against Basidiomycete fungi
2008 - IRG/WP 08-10645
The aim of this research was to study the response of mixed plywoods composed of durable heartwood and non-durable sapwood to fungal attack. For this purpose, the species of Cupressus sempervirens (cypress) was chosen for its well-known durable heartwood. The evaluation of durability of plywood panels against fungi was done according to ENV 12038 and the fungi used were Coriolus versicolor (CV) an...
F Faraji, M F Thévenon, N Lemenager, B Thibaut


Wood decay fungi from New Zealand leaky buildings – PCR identification (Part 2) and aerial spore trapping
2008 - IRG/WP 08-10649
Prior to this study, it was not know which species of decay fungi caused decay in New Zealand leaky buildings. Use of molecular biology methodology, polymerase chain reaction (PCR), and subsequent DNA sequencing, as well as classical mycological techniques based on morphology, has enabled identification of decay fungi and has provided insight into their relative importance based on isolation frequ...
D Stahlhut, R L Farrell, R Wakeling, M Hedley


Decay resistance of propionylated Iranian beech against the white rot fungus Tramets versicolor
2008 - IRG/WP 08-30449
In this study, the decay resistance of propionylated beech was investigated against the white rot fungus Trametes versicolor. Iranian beech (Fagus orientalis) was modified with propionic anhydride at different temperatures, concentrations and times without using any catalyst. Different weight percentage gains (WPGs) were obtained under different conditions. The decay resistance of the esterifed wo...
M R M Farahani, S M Hosseini


Efficacy of Neem (Azadirachta indica) Leaves Against Wood Decay Fungi
2008 - IRG/WP 08-30450
Many plant-derived substances have fungitoxic, pecticidal and insecticidal ability. Neem leaves are very popular in this respect since the ancient time. The present study is to investigate the fungitoxic activities of neem leaves extractives against wood decay fungi. Three different solvents, i.e. acetone, methyl alcohol, ethyl alcohol, were used to isolate the extract of neem leaves. The extracti...
A Humayan Kabir, M A Rahman, M Firoz Alam


Antifungal activity of different molecular weights of a biopolymer chitosan against wood decay fungi
2008 - IRG/WP 08-30456
In recent years chitosan has been investigated as a natural polymer for wood preservation against fungal decay. From an environmental point of view, chitosan seems to possess a potential approaches as wood protecting agent. In this study, three different molecular weights of chitosan compounds (with approximately the same degree of deacetylation 83 ± 2 %) were evaluated as in vitro and in vivo as...
A S O Mohareb, M E I Badawy


In-service performance of wood depends upon the critical in-situ conditions. Case studies.
2008 - IRG/WP 08-20382
Wood is a unique building material, but is by nature designed to deteriorate. A detailed understanding of the factors and interactions involved are important when working with service life prediction of wooden components in buildings. Wood may experience exponential fungal degradation caused by variation in the climatic factors within a small limited area and by minor imperfection in the wooden ...
L Ross Gobakken, J Mattsson, G Alfredsen


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