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Hydrolysis of bordered pits during colonization of conifers by brown-rot fungi
1995 - IRG/WP 95-10103
Brown-rot decay results in rapid reduction in degree of polymerization (DP) of holocellulose with concomitant strength loss (MOR) without removing lignin. Development of new methods of wood protection will require focusing on early events in the sequence of depolymerization. Bordered pit membranes (sapwood) represent a readily available source of non-lignified carbohydrate, ie. pectin and cellulos...
F Green III, J L Tschernitz, T A Kuster, T L Highley


Studies and experiences of occurrence and development of soft rot in salt-treated poles of pine (Pinus sylvestris) installed in Swedish transmission lines in the years 1940-1945
1977 - IRG/WP 277
The present work describes the result of microscopic examinations of some 2,000 borings extracted from 20 to 23-year-old salt-treated poles (Scots pine) from the southern and central parts of Sweden. The purpose was to map out the occurrence of soft rot in Sweden and its influence on the mechanical strength of the poles as well as to contribute to the development of new and more satisfactory metho...
H Friis-Hansen


The involvement of extracellular substances for the generation of hydroxyl radical during wood degradation by white-rot fungi
1997 - IRG/WP 97-10218
The activities of one-electron oxidation, hydroxyl radical generation, and phenol oxidase during the incubation of cultures of four white-rot fungi containing either glucose or wood were periodically measured. Further, their degradation activities against wood were examined during the course of cultivation. The generation of hydroxyl radical was correlated to the activity of wood degradation and i...
H Tanaka, S Itakura, A Enoki


Antagonistic properties of Gliocladium virens against wood attacking fungi
1996 - IRG/WP 96-10162
Gliocladium virens has shown good antagonism against decay fungi in agar medium and in wood blocks. Gliotoxin produced by Gliocladium virens is associated with biocontrol of some plant diseases, but its importance to biocontrol of wood-attacking fungi is unknown. We investigated the ability of gliotoxin-producing (GLT+) isolates of Gliocladium virens and gliotoxin-deficient (GLT-) mutants of Glioc...
T L Highley, H S Ananthapadmanabha, C R Howell


The importance of blue stain attack for the colonization by wood-rotting fungi of wood not in contact with the ground
1988 - IRG/WP 1349
When used in constructions not in contact with the ground, wood has been shown often to proceed from blue stain to moulds. The appearance of wood rotting fungi is normally delayed. Solid wood artificially inoculated with the blue stain fungus Pullularia pullulans was shown to permit germination of Pycnoporus cinnabarinus basidiospores. This was demonstrated by the use of an indirect and a direct b...
J Bjurman


Evaluation of white-rot fungal growth on Southern Yellow pine wood chips pretreated with blue-stain fungi
2000 - IRG/WP 00-10349
White-rotting basidiomycetes do not colonize on southern yellow pine. This study seeks to reduce the resinous extractive content of southern yellow pine by treating it with blue stain fungi. The mycelial growth of wood-inhabiting ligninolytic white-rot fungi can be achieved on pretreated southern yellow pine wood. Aureobasidium, Ceratocystis, and Ophiostoma spp. removed 70% to 100% of the extracti...
S C Croan


Soft rot tests with soils of different origins
1984 - IRG/WP 2226
In the BAM a comparative soft rot test with 9 different types of soil (one soil each from Canada, New Zealand and Switzerland and 6 soils from laboratories in Germany) were carried out. The pH values of the different soils ranged from 3.2 to 7.1, and the amounts of organic matter were between 4 and 44% (w/w). The test specimens - Pine sapwood blocks treated with different concentrations of a CCA p...
M Gersonde, W Kerner


An unusual soft-rot decay pattern caused by the Ascomycete Hypoxylon mediterraneum (de Not.) J Miller
1984 - IRG/WP 1222
A distinct pattern of soft-rot decay has been observed for the fungus Hypoxylan mediterraneum (de Not.) J. Miller. This fungus also produced decay patterns typical of brown and white rot decay. The production of characteristic cavities by Hypoxylan mediterraneum was prolific in the hardwoods Eucalyptus maculata and Eucalyptus regnans, but infrequent in the softwoods, Pinus elliottii and Pinus radi...
D M Francis, L E Leightley


Soft rot test of copper/chrome/arsenic treated heartwood of three Malaysian timbers by the vermiculite-burial method. (+ correction document of 25 July 1990)
1990 - IRG/WP 2354
Heartwood of copper-chrome-arsenic (CCA) treated kempas (Koompassia malaccensis), tualang (Koompassia excelsa) and keruing (Dipterocarpus spec.) was found to be susceptible to soft rot in recent pole surveys. Standardized heartwood blocks were impregnated with 0 to 6.3% (w/v) CCA and challenged to decay for twelve weeks by a mixed inocula of Chaetomium globosum, Glenospora graphii, Humicula grisea...
R-D Peek, A H H Wong


Objectives of future collaborative soft rot tests
1972 - IRG/WP 209
Progress has been made towards acceptance of a defined laboratory test method for determining the toxicity of preservatives against soft rot fungi (Documents IRG/WP/201 and IRG/WP/208) and, at the 1971 meeting in Brussels, the framework of collaboration for the next phase of testing was discussed. Time available at Brussels did not permit restatement of objectives, consideration of the stages by w...
J G Savory


Evaluation of a laboratory soft rot test on basis of weight loss and residual strength
1989 - IRG/WP 2336
Based on the principles of different previously reported experimental procedures dealing with laboratory soft rot testing, a study was conducted on treated poplar and beech. Preservatives used were CCA, CCF, AAC, Azaconazole and TCMTB. Treated samples were exposed for three months in a vermiculite substrate to Chaetomium globosum. Evaluation was based on absolute mass loss, percentage weight loss ...
W Verbeke, J Van Acker, M Stevens


The development of soft rot decay in bamboo fibres
1992 - IRG/WP 92-1572
The development of decay by soft rot fungus Chaetomium globosum was investigated in young and mature culms of bamboo Phyllostachys virideglaucescens. Soft rot decay was influenced both quantitatively and qualitatively by the differing levels of lignification in the material. Weight loss was significantly greater in the younger culm compared with the older, more heavily lignified culm. The distribu...
O Sulaiman, R J Murphy


Potential toxicants for controlling soft rot in preservative treated hardwoods. Part 2: Laboratory screening tests using sawdust
1977 - IRG/WP 2101
A technique is described for the rapid screening of toxicants against microorganisms, represented by the brown rot, Gloeophyllum trabeum, and a natural inoculum from soft rotted wood. Sawdust, in this case from sapwood of Eucalyptus maculata, is pretreated with the candidate formulation and moistened before inoculation. Visual scoring of growth can be used to discriminate suitable candidates as ea...
J D Thornton


Screening of fungal strains for wood extractive degradation
1998 - IRG/WP 98-10254
Fungal strains were screened for their ability to degrade apolar extractives in wood from scots pine. The degradation of total wood extractives by 91 different strains was monitored in stationary batch assays incubated for 6 weeks. The results obtained show that the ability of wood-inhabiting fungi to utilize wood extractives varied greatly, even for different isolates of the same species. Fungal ...
J Dorado, M J Martinez-Inigo, T A van Beek, F W Claassen, J B P A Wijnberg, R Sierra-Alvarez


Studies in an accelerated soil bed facility on the decay susceptibility of U.K. grown spruce and pine poles treated with copper/chrome/arsenic (CCA) by pressurised sap-displacement. Part 1: Setting up of soil beds and initial soft rot results
1990 - IRG/WP 2344
The paper describes the methodology used in the construction and early operation of an accelerated soil bed facility used to examine the decay susceptibility of U.K. grown Scots and Corsican pine and Sitka and Norway spruce treated with C.C.A. by high pressure sap-displacememt. The design and control of the facility as well as the preparation, soil exposure and soft rot decay analysis of quarter p...
A Bruce, S D Hainey, G M Smith, B King, P D Evans


Soft-rot testing. Memo to CEN
1983 - IRG/WP 2206 A
1. Development of a single test procedure to assess performance of preservatives against soft rot fungi is an ideal that cannot be realised at present, if results are to be both reproducible between laboratories and pertinent to the practical requirements of individual countries. 2. Consensus opinion amongst members of Sub Group I - Soft rot tests, of Working Group II - Fundamentals of Testing, of the IRG, is that the immediate needs of CEN would be best answered by: (a) a code of recommended practice, (b) a multiplicity of test procedures, and (c) a degree of flexibility in test procedures. 3. The code of recommended practice should recognise the need for: (a) a pure culture, synthetic medium (sand or vermiculite + nutrients), single wood species test to be adopted by all laboratories as a common reference point of testing, (b) mixed inoculum and/or unsterile soil laboratory or fungus cellar tests which account for local variations in the fungal flora, (c) incorporation of a range of wood species where applicable (i.e. where mixed hardwoods comprise the main resource), (d) provision of local internal standards of known performance under field conditions, (e) incorporation of leaching procedures other than standard methods with deionized water (e.g. mild acid, milk alkali, ionic solutions of various salts) where applicable to local exposure conditions, (f) precise definition of the criteria used for establishing toxic thresholds (mass loss, strength loss, microscopic examination). 4. Initial screening tests should remain the province of the individual research worker. 5. The code of recommended practice should have the sole purpose of establishing provisional retention levels for subsequent confirmation by field trial. 6. IRG should list and approve test procedures considered adequate to establish toxic thresholds for preservatives against soft rot fungi. 7. It should be clearly understood that when using a series of test techniques, it is inevitable that a range of toxic thresholds will be obtained for the same preservative. 8. Guidelines should be drawn up for interpretation of test data for subsequent transformation to provisional retention levels. 9. Adoption of the broad principles outlines above is recognition of the dynamic nature of toxic thresholds. The additional information obtained by a more flexible, and complex, approach to testing may make interpretation more difficult but should provide data from which response of preservatives to varying biological and environmental hazards can be more realistically assessed. 10. It is recommended that CEN adopt, in principle, the basic philosophy of testing outlined above and move towards formulation of a Working Document in collaboration with IRG
J A Butcher, D J Dickinson


Free radical process controlled by manganese peroxidase and lipid-related metabolites produced by Ceriporiopsis subvermispora
2001 - IRG/WP 01-10412
Ceriporiopsis subvermispora, a specific lignin-degrading fungus produced free unsaturated fatty acids (USFAs) including 9,12-octadecaienoic asid, together with saturated fatty acids (SFAs) at an incipient stage of cultivation on wood meal cultures. In prolonged cultivation period after two weeks, the amount of intact fatty acids decreased with increasing in organic hydroperoxide and TBARS producti...
T Watanabe, M Enoki, S Sato, Y Honda, M Kuwahara, N Shirai, K Messner


Effect of fungal degradation on the chemical composition of acetylated beech wood
2003 - IRG/WP 03-40267
This study investigated the impact of fungal attack on the chemical composition of acetylated wood. Beech wood acetylated to different degrees was exposed to decay by the white-rot fungus Trametes versicolor under solid-state fermentation conditions. Laboratory soil-bed assays were also conducted to study the degradation of acetylated wood by soft rot fungi and other soil-inhabiting microorganisms...
H Militz, Dong-won Son, L Gómez-Hernández, R Sierra-Alvarez


The microbial ecology of treated birch stakes in a soil-bed
1983 - IRG/WP 1209
The microbial ecology of small birch stakes exposed in soil beds in two Fungus Cellars was investigated. Patterns of fungal colonisation and decay initiation are described in untreated birch and copper fluosilicate-treated stakes. Results are based on fungal isolation onto selective media and direct light microscopical observations of decay features. In untreated stakes there was a rapid build-up ...
C P Clubbe


Factors affecting decay rates in a fungus cellar
1985 - IRG/WP 2242
Birch and pine stakes treated with chromated copper arsenate and didecyldimethylammonium chloride were incubated in two soils in a "Fungus Cellar" test. At three month intervals, sets of stakes were inspected and assigned visual ratings or removed from the soil beds for weight loss determination. Preliminary results after nine months exposure have shown differences in the decay rates between birch...
P A McKaig


Extracellular carbohydrate production by isolates of Postia (=Poria) placenta
1989 - IRG/WP 1388
A monokaryotic strain of Postia (=Poria) placenta, ME20, which is unable to degrade wood, also failed to produce extracellular polysaccharide when grown in liquid culture, regardless of carbon source or concentration. Other isolates of Postia placenta, including another monokaryon and a hybrid of this monokaryon with ME20, produced large quantities of this material. The polysaccharide consisted pr...
J A Micales, A L Richter, T L Highley


Moisture Adsorption Behaviour of Rubber Wood Decayed by Brown and White rot Fungi
2005 - IRG/WP 05-40306
Moisture adsorption behaviour of rubber wood blocks decayed by each brown rot and white rot fungi was studied. The adsorption isotherms of decayed wood were compared with the isotherm of undecayed wood. The wood decayed by brown rot adsorbed less moisture as compared to sound or undecayed wood at all humidity levels. No significant difference was observed in adsorption behaviour of white rot dec...
S S Chauhan, H C Nagaveni


Collaborative soft rot tests: Specific gravity loss resulting from soft rot
1973 - IRG/WP 227
It was proposed at the 5th meeting of Working Group II of the IRG in June 1973 in Lahti, Finland, that the specific gravity (swollen volume basis) of test blocks used in the IRG Collaborative Soft Rot Tests should be determined on all the blocks at the completion of the exposure period. Specific gravity determined on the basis of the oven dry weight and water swollen volume was selected because (1...
C R Levy


The use of organic wood preservatives in ground contact and the suitability of laboratory test procedures to determine their efficacy
1999 - IRG/WP 99-20175
Organic fungicides, such as propiconazole, have proven to be highly effective when used as a co-biocide in recently developed wood preservative formulations. They have however been ineffective when used as a stand alone preservative, particularly when used in ground contact (European Hazard class 4). Previous research, using the test methods of ENV 807 (1993), provided conflicting evidence regardi...
I J Herring, D J Dickinson


Mycoparasitism by some white rot fungi on blue stain fungi in culture
1986 - IRG/WP 1304
When studying biological control of blue stain an interesting phenomenon of parasitism by some white rot fungi on blue stain fungi has been encountered. The majority of the 22 tested white rot fungi act parasitically upon blue stain fungi. The most interesting species among them is Bjerkandera adusta (Willd. ex Fr.) Karst., causing almost complete or complete decoloration of the hyphae of the blue...
R Benko, B Henningsson


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