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Remedial ground-line treatment of CCA poles in service. Results of chemical and microbiological analyses 6 months after treatment
1986 - IRG/WP 3388
CCA-treated poles in service with incipient internal soft rot were remedially treated by inserting borate rods, brushing with a boron/glycol solution and injecting boric acid paste, copper/creosote paste or a commercial product (DFCK paste). The spread of active chemicals in the treated zone as well as the change in microflora have been studied with time. After six months chemicals had spread to m...
B Henningsson, H Friis-Hansen, A Käärik, M-L Edlund


The antifungal efficacy of Guayule resin
1987 - IRG/WP 3429
The Naval Research Laboratory is evaluating the non-rubber-producing portion of guayule (Parthenium argentatum) resin as a protectant for wood in terrestrial and marine service. This study phase, in collaboration with the universities of Arizona and Mississippi State, evaluates the resin's fungicidal worth. Resin-impregnated pine sapwood was exposed to brown rot fungi (Gloeophyllum trabeu...
J D Bultman, R L Gilbertson, T L Amburgey, J E Adaskaveg, S V Parikh, C A Bailey


Effect of microfibril orientation of bamboo cell wall on soft rot penetration hyphae
1994 - IRG/WP 94-10087
The effect of microfibrillar orientation of bamboo (Phyllostachys virideglaucescence) cell wall on the development of soft rot (Chaetomium globosum) penetration hyphae was investigated. It was found that the soft rot penetration hyphae normally followed the microfibril angle of the cell wall. Bamboo cell walls have alternating broad and narrow lamellae with different microfibrillar angles. The mic...
O Sulaiman, R J Murphy


Oxalic acid quantification, oxaloacetase assay and ESI localization of P, C, and Fe from the brown rot fungus Postia placenta
1994 - IRG/WP 94-10063
The mechanism by which brown-rot fungi initiate depolymerization of holocellulose in wood remains unknown. Recently, oxalic acid (OA) has received considerable attention in cellulose breakdown by brown-rot fungi. The OA could serve as a proton donor for hydrolytic or an electron donor for oxidative (Fenton's reaction-H2O2/Fe2+) cleavages of cellulose. The acid may originate via oxaloaceta...
C R Jordan, W V Dashek, T L Highley


Chlorothalonil: A new ground contact wood preservative
1992 - IRG/WP 92-3712
Extensive investigations have been conducted in recent years on the performance of chlorothalonil (tetrachloroisophthalonitrile) as a ground contact wood preservative. Efficacy information is presented on a soil block test with 11 Basidiomycetes, a fungus cellar evaluation, above-ground field testing against termites in Australia and Hawaii, and ground-contact field stakes installed in Florida (9 ...
P E Laks, T L Woods


Proposed model for the penetration and decay of wood by the hyphal sheath of the brown-rot fungus Postia placenta
1989 - IRG/WP 1391
Scanning electron microscopy (SEM) of Pinus sp. decayed by the brown-rot fungus Postia placenta confirmed the existence of extracellular membranous structures previously described by transmission electron microscopy (TEM). These structures appear to be an integral part of the hyphal sheath and assume a variety of forms including lamellar sheets, fibrils, and vesicles. These structures were observe...
F Green III, M J Larsen, L L Murmanis, T L Highley


Early detection of brown-rot decay in southern yellow pine using immunodiagnostic procedures
1990 - IRG/WP 2356
Immunodiagnostic procedures have been used to detect incipient decay of southern yellow pine by six common brown-rot fungi. Rabbit polyclonal antibodies were raised to liquid culture fractions of the six fungi. The antibodies, after preadsorption to sawdust, were tested in particle agglutination assays, immuno-dot blot, and ELISA for their·ability to detect the decay organisms when they were grow...
C A Clausen, F Green III, T L Highley


Soft-rot control in hardwoods treated with chromated copper arsenate preservatives. Part 3: Influence of wood substrate and copper loadings
1977 - IRG/WP 2100
The hypothesis is proposed that hardwoods need more chromated copper arsenate (CCA) than softwoods to protect them from soft-rot attack mainly because hardwoods are more readily consumed by soft-rot fungi. Simple model systems, using copper-supplemented agar or groundwood pulp treated with CCA showed that fungi tolerated more toxicant (copper) as more available substrate (malt) was provided. Soft-...
M A Hulme, J A Butcher


Detection of a brown-rot fungus using serological assays
1986 - IRG/WP 1305
Polyclonal antisera produced to Poria placenta (Fr.) Cooke were used in two tests to qualitatively assay for the fungus. Fungal hyphae were fixed to slides and fluorescent antibody (FA) techniques used to visualize the hyphae under the microscope. Fluorescence of non-Poria fungi, when present, could be reduced but not eliminated by cross-absorbing the sera with these fungi. The antisera was also u...
B Goodell, J Jellison


A laboratory technique to measure the performance of preservative treated hardwoods in ground contact
1982 - IRG/WP 2172
A laboratory technique to measure the performance of preservative treated timber in ground contact is described. It uses partially sealed blocks of timber which are treated, leached and sawn into small stakelets for soil exposure in a fungal cellar. Performance is monitored by the loss of static bending strength with time, and a simple apparatus for measuring the deflection of a stakelet under a l...
E F Baines


Enhanced biodegradation of cocopeat by soft rot fungi
1998 - IRG/WP 98-10276
Biodegradation of cocopeat (coir dust) was enhanced by the addition of nitrogen (N) fertilizer and inoculation with the soft rot fungus Chaetomium globosum. The N and fungally treated cocopeats had a greater percentage weight loss (27%) after 3 months compared with 7% weight loss without the added fungi and N fertilizer. In addition, their hemicellulose, cellulose and lignin contents were greatly ...
P Y Yau, R J Murphy


Protection of southern pine using N,N-Napthaloylhydroxylamine: Field tests, soft-rot cellars and aquatic bioassay leach testing
1999 - IRG/WP 99-30204
Recent environmental restrictions are limiting the use of broad-spectrum biocides for wood preservation. There is an urgent need for new, sharply targeted, environmentally benign wood preservatives. N'N-Napthaloylhydroxylamine (NHA), a water-soluble calcium-precipitating agent, has been shown to inhibit decay by brown-rot and white-rot fungi in soil-block tests and prevent damage by Easte...
D M Crawford, F Green III


Hardwood field experiment: Progress report 1977-82
1982 - IRG/WP 3200
The international hardwood field experiment was planned in 1976 and set up in some 30 different sites around the world. The test stakes include 4 reference species common to each site and in most cases at least 2 species of local importance. It was hoped that a picture of performance of a range of economically important species would be built up and at the same time provide vital background inform...
D J Dickinson, J F Levy


Diffuse cavity formation in soft rot of pine
1992 - IRG/WP 92-1541
A new type of soft rot of southern pine longitudinal tracheids is described. In this type, soft rot cavities form by diffuse degradation of the S2 cell wall layer by hyphae growing within the cell wall. Erosion is diffuse and irregular as opposed to the restricted, periodic erosion typical of type 1 soft rot cavity formation. Proboscis hyphae remain small (diameter 0.6 to 0.8 µm) and rapidly auto...
S E Anagnost, J J Worrall, C J K Wang


Resistance of acetylated wood to basidiomycetes, soft rot and blue stain
1994 - IRG/WP 94-40021
Poplar (Populus spp.), beech (Fagus sylvatica) and pine sapwood (Pinus sylvestris) samples were acetylated in a semi-industrial acetylation plant and tested for durability according to European standards. Resistance to Gloeophyllum trabeum, Coniophora puteana and Coriolus versicolor could be reached at a weight percent gain (WPG) of 12%. Susceptibility of acetylated pine to Poria placenta was high...
E P J Beckers, H Militz, M Stevens


Decay resistance of densified ammonia-plasticized stems of oil palm (Elaeis guineensis)
1991 - IRG/WP 3673
When wafers of oil palm stems (Elaeis guineensis) were plasticized with 28% aqueous ammonia and immediately compressed mechanically, the treated material, gained 73% in basic density (average basic density, 0.695 g/cm³), and was highly resistant to decay by wood rot basidiomycetes. Compared with the control specimens (density, 0.403 g/cm³), resistance to decay of the densified specimens by Corio...
A H H Wong, M P Koh


Dimensional lumber model demonstrates the sensitivity of the particle capture immunoassay in early detection of brown-rot fungi
1995 - IRG/WP 95-20058
A dimensional lumber model was designed to test the sensitivity of the particle capture immunoassay (PCI) compared to traditional methods of culturing and microscopic examination for detection of brown-rot fungi. Southern yellow pine 2 x 4's (5.1x10.2 cm²), and 86.4 cm in length, were inoculated on one end with the brown-rot fungus, Postia placenta, and tested over the full length of the...
C A Clausen, L Ferge


Decay resistance of Platanus acerifolia wood against White Rot Fungi (Coriolus versicolor)
2002 - IRG/WP 02-10431
Degradation of the lignocellulose complex in wood varies on the microorganism causing decay and chemical composition of wood. The weight loss of Platanus acerifolia wood after decay by white rot fungus (Coriolus versicolor) for period 16 weeks were evaluated according to European standard EN 113 and beech wood as a control. The samples were collected in October 2000 from Nantes, France. The aim of...
M Irmouli, O Duchelier, N Ayadi, B Charrier


The long road to understanding brown-rot decay. A view from the ditch
1995 - IRG/WP 95-10101
Interest in understanding how brown-rot fungi decay wood has received increasing interest in recent years because of a need to identify novel targets that can be inhibited for the next generation of antifungal wood preservatives. Brown-rot fungi are unique in that they can degrade holocellulose (cellulose and hemicellulose) in wood without first removing the lignin. Furthermore, they degrade holoc...
F Green III, T L Highley


Addendum to paper for discussion in Working II
1970 - IRG/WP II 5B
In view of the limited time that will be available for discussions in Nancy, this addendum has been prepared. Arising from the previous collaborative work and Mr Bravery’s visit to most of the co-operating Institutes, a scheme of work entailing tests carried out 1) by a technique chosen by individual Institutes; 2) by an agreed standardised technique would appear to be the best approach. A compl...
J G Savory, A F Bravery


Micromorphology of Schizophyllum commune attack in pine (Pinus sylvestris) wood
1983 - IRG/WP 1184
The decay pattern caused by Schizophyllum commune in pine latewood tracheids was studied using both light and transmission electron microscopy. The attack began as isolated concentric slits within the S2 layer with extensive lamellation and separation of individual wall layers observed in subsequent stages of decay. The slits resulting from attack appeared to be formed in thin, regular concentric ...
T Nilsson, G F Daniel


Targeted inhibition of wood decay (Using everything but the kitchen sink)
1997 - IRG/WP 97-10203
Low molecular weight oxidative decay agents have been implicated in the degradation of wood by brown-rot decay as evidenced by chemical analysis of brown-rotted wood and detection of oxalic acid and hydroxy radicals. Fenton chemistry (H2O2 / Fe++) is often proposed as the mechanism for generating hydroxy radicals. Previous authors have shown iron to enhance the brown-rot hydrolysis of wood, while ...
F Green III, T A Kuster, T L Highley


Effect of soil type and moisture content on soft rot testin
1986 - IRG/WP 2270
Several soils of different types were tested at a range of moisture contents for their suitability for use in a soil burial soft rot test. Their effectiveness was assessed by weight loss in CCA treated and untreated beech and birch outerwood. A horticultural loam (John Innes No. 2) was the most appropriare soil in terms of amount of weight loss caused, tolerance to changes in soil moisture content...
S M Gray


Incidence of soft rot in creosoted poles
1992 - IRG/WP 92-1554
A further population of creosote-treated poles has been assessed for the occurrence of soft rot, as a continuation of work reported in IRG Document No. IRG/WP/1368. The outer 1 cm of each core was assessed microscopically and graded for the presence of soft rot cavities in the wood cell walls; with further assessments taken at a consecutive 1cm interval for cores showing positive findings. Followi...
D J Dickinson, P W McCormack, B Calver


Destruction of two tropical timbers by marine borers and micro-organisms in Goa waters (India)
1992 - IRG/WP 92-4176
The paper deals with the pattern of microbial attack in relation to marine borer damage of test panels of African rosewood and Brazilian jackwood exposed in Goa waters (Western India) for a period of seven months. The degradation of wood cell walls in both the timber samples occurred due to infestation of soft-rot fungi and tunnelling bacteria. African rosewood, which was severely damaged by tered...
L N Santhakumaran, A P Singh


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