Your search resulted in 111 documents. Displaying 25 entries per page.
Detection of the dry rot fungus Serpula lacrymans by amplified ribosomal DNA restriction analysis
1998 - IRG/WP 98-10245
Isolates of the dry rot fungus Serpula lacrymans and the wild merulius S. himantioides were investigated by amplified ribosomal DNA restriction analyses (ARDRA) of the internal transcribed spacers (ITS). The technique uses the polymerase chain reaction (PCR) to amplify the variable region of the ITS between the conserved 18S and 28S genes of the nuclear ribosomal DNA (rDNA).
The ITS region of all isolates of Serpula lacrymans and S. himantioides was successfully amplified. The length of the amplified product was about 630 bp. Subsequent double digest of the ITS with the restriction endonucleases Haelli and Taql separated the closely related fungi by species-specific fragments. Thus, ARDRA-ITS proved to be suited for the detection of the dry rot fungus.
O Schmidt, U Moreth
Questionnaire relating to the occurrence, prevention and repair of Dry Rot attacks in buildings
1989 - IRG/WP 1418
Dry Rot attack in buildings has become an increasing problem in Denmark. The reason is that old multi-storey houses from about 1890-1920 are being renovated and modernised and connected to this well-hidden Dry Rot attacks are detected. The traditional methods for repair used in Denmark are very expensive but with a high degree of confidence when done carefully. There is a big need for development ...
A P Koch
A comparison analysis of eight strains of Serpula lacrymans (Schum. ex Fr.) S.F. Gray
1991 - IRG/WP 2362
Investigations were previously carried out to compare eight strains of Serpula lacrymans (Schum. ex Fr.) S.F. Gray (some used in various countries as standard test strains): FPRL 12C (England), FPRL 12E (Germany), Warsaw III (Poland), HFP 7802 (Japan), DFP 16508, 16509, 16521 and 16522 (Australia). Studies included growth rate and dry mass of mycelium, decay capacity, reduction of compression stre...
J Wazny, J D Thornton
Fungal degradation of wood treated with metal-based preservatives. Part 1: Fungal tolerance
1996 - IRG/WP 96-10163
In recent years, concerns have arisen about the leaching of heavy metals from wood treated with chromated copper arsenate (CCA), particularly because of the large amount of CCA treated wood that will be discarded in the coming years. The long term objectives of this work are to determine the fate of copper, chromium and arsenic with the aging and potential decay of CCA-treated wood, and to develop...
B Illman, T L Highley
Biological variability in the oxalate/oxalate decarboxylase system among five isolates of the wood-degrading fungus Meruliporia incrassate
2006 - IRG/WP 06-10573
The “dry-rot” wood decay fungus Meruliporia incrassata has recently attracted attention, primarily in the western coastal United States, as a particularly destructive pest of building material. Recently, the US Environmental Protection Agency (EPA) has accepted a voluntary withdrawal of the historically effective chromated-copper arsenate (CCA) as a preservative for wood used in residential se...
C Howell, J Jellison
The use of X-ray diffraction for analyzing biomodification of crystalline cellulose by wood decay fungi
2007 - IRG/WP 07-10622
X-ray diffraction (XRD) is based on the creation of an interference pattern by x-rays when they encounter a regularly spaced matrix. In wood, this process has been used to determine, among other things, the average width of the cellulose microcrystals, the percent of crystalline cellulose within the wood, and can be used to examine the changes in these parameters during degradation. Enhanced under...
C Howell, A C Steenkjær Hastrup, J Jellison
Gypsum effects on ‘dry rot’ wood degradation as a function of environment
2007 - IRG/WP 07-10624
‘Dry rot’ fungi are a unique group of brown rot fungi that can degrade wood away from ground contact where other fungi fail to colonize. Successfully occupying this niche is partially due to efficient water and nutrient transport, but mobilizing elements, notably calcium (Ca) and iron (Fe), from adjacent building materials has also been implicated in their success. Here we report a series of t...
J Schilling, J Jellison
Serpula lacrymans – calcium, iron, and foundering wooden boats
2009 - IRG/WP 09-10691
Serpula lacrymans is one of the most destructive wood-degrading brown rot fungi in temperate environments. Its virulence has often been linked to its ability to grow over non-woody materials and extract calcium (Ca) or iron (Fe) to promote wood degradation in buildings. This fungus has also been a severe problem in historic wooden warships and in modern wooden vessels, sometimes leading to founder...
J S Schilling, S M Duncan
Effects of cold treatment on wood destroying fungi important in cultural heritage
2009 - IRG/WP 09-10706
The dry rot fungus Serpula lacrymans (Wulfen:Fr.) Schroeter is notorious in building environments for its vitality, destructive potential, and the huge costs associated with remediation of damage caused to wood of cultural heritage. Due to the potentially adverse of effects of chemical treatment methods such as have been attempted in the past, more benign physical methods of treatment, e.g. heat t...
M Lüdicke, W Unger, G Binker
Profiling fungal community in wood decay ecosystem by Denaturing High-Performance Liquid Chromatography
2010 - IRG/WP 10-20443
A DNA-based fingerprinting technique, Denaturing High Performance Liquid Chromatography (DHPLC) was developed to profile fungal communities colonizing indoor timber. Molecular fungal diversity was assessed using amplification based on the Internal Transcribed Spacer (ITS1) rDNA of 74 wood samples collected from infected buildings in France. Due to its high sensitivity, the PCR-DHPLC technique was ...
S Maurice, G Le Floch, M Le Bras-Quéré, J P Rioult, G Barbier
Real-Time PCR Assays for the detection of the dry rot fungus Serpula lacrymans and its close relative Serpula himantioides
2016 - IRG/WP 16-10867
Prevention of wood decay by fungi of the phylum Basidiomycota is an important issue of wood protection. The most destructive fungus in buildings in Central Europe is the dry rot fungus Serpula lacrymans, which needs more extensive restauration measures than all other wood decay fungi. Its close relative S. himantioides occurs more frequently in outdoor environment. The assessment and remediation o...
K Jacobs, N Rangno