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Temperature tolerance of Botryodiplodia theobromae causing sapstain on rubber wood
1998 - IRG/WP 98-10259
Botryodiplodia theobromae Pat. is the dominant fungus causing sapstain on tropical timbers. Rubber wood (Hevea brasiliensis) is highly susceptible to sapstain infection. The tropical warm-humid climate also influences the growth of B. theobromae on rubber wood. Additionally, the moisture content of timber provides conducive conditions for the entry and establishment of the fungus. Temperature tole...
E J M Florence, J K Sharma, R Gnanaharan


Field trials of Sinesto B at some sawmills in Portugal
1989 - IRG/WP 3512
The efficacy of SINESTO B as antistain chemical for use in Portugal has been studied. The studies were done during the years 1987-1988 at several Portuguese sawmills in co-operation with the Instituto dos Produtos Florestais and the APCIM (Associacao Portuguesa de Comercio e Industria de Madeiras). SINESTO B was used in 5% and 8% concentrations. The storage time varied from 10 weeks to 6 months. I...
I A Linderborg


Evaluation of a new anti-sapstain formulation
1994 - IRG/WP 94-30035
A new anti-sapstain mixture, which consists of 2% IPBC (3-iodo-2-propynylbutyl carbamate) and 1.5% DCOI (4,5-dichloro-2-n-octylisothiazolin-3-one), was evaluated by three methods in the laboratory. A standardized test (JWPA standard 2) demonstrated that the new anti-sapstain formulation was highly effective in controlling growth of monocultures of five test fungi on wood substrate. When exposed to...
K Tsunoda, H Kumagai, M Sakurai


A shower test protocol for measuring antisapstain wash-off from small individual log billets
1996 - IRG/WP 96-50069
According to New Zealand Timber Industry Federation statistics New Zealand exported 4,835,000 m³ of logs in 1994 and it is estimated that c. 20% were anti-sapstain treated. Loss of preservative from logs as a result of rain impact after application is of considerable concern both with respect to potential loss of efficacy and environmental contamination. Whilst Environment Canada have a proposed ...
A J Pendlebury, H Pearson


Mold and stain fungi associated with Radiata pine logs imported from New Zealand
2000 - IRG/WP 00-10346
The Korean sawmills have recently realized the importance of prevention of fungal discoloration due to increased losses in revenue. Before establishing integrated control strategies of fungal discoloration, more complete knowledge about causal organisms is needed. As a first step, we initiated a through survey of mold and stain fungi colonized commercially important softwood species (Pinus densifl...
Jae-Jin Kim, Gyu-Hyeok Kim


Differential susceptibility of living and dead timber to colonisation by sapstain and mould fungi
1998 - IRG/WP 98-10288
Field studies have revealed that when timber is irradiated (i.e. killed) it is more susceptible to colonisation by mould fungi than sapstain fungi. By comparison, freshly sawn timber shows very little mould colonisation, tending to be colonised by sapstain fungi. It appears, therefore that the physiological state of the wood may influence the pattern of colonisation. A laboratory trial was underta...
J R Williams, D J Dickinson, J F Webber


Laboratory trial to identify potential in-forest treatments to control fungal pre-infections of radiata pine logs
2000 - IRG/WP 00-30220
Development of fungal infections of radiata pine logs by wood degrading fungi commences immediately after tree felling. In general, subsequent antisapstain treatments are unable to control fungal degrade on logs where the pre-treatment log storage time exceeds 2-5 days. However, use of an in-forest treatment of logs may be advantageous to log exporters to control fungal pre-infections during the p...
D R Eden, B Kreber, R N Wakeling, J G Van der Waals, C M Chittenden


Effect of bioextracts on colonisation of radiata pine sapwood by three sapstain fungi
2003 - IRG/WP 03-10485
The objective of this study was to investigate the effect of aqueous bioextracts prepared following two-week incubation of forest soil, garden compost and chicken manure respectively, on the ability of Ophiostoma flocossum, Leptographium procerum and Sphaeropsis sapinea to colonise unseasoned radiata pine sapwood. The research showed that all bioextracts tested retarded the rate of fungal colonis...
J van der Waals, C Chittenden, B Kreber


Susceptibility of painted wood to discolouring fungi - influence of binder, solvent and surfactant
1992 - IRG/WP 92-3714
Previous studies have shown that the basic composition of paints, without fungicide addition, can significantly contribute to the varying microbial susceptibility of painted wood. Previously, non-weathered water-borne acrylic paints applied to wood without a primer were shown to be more susceptible to mould attack than solvent-borne alkyd paints. The present study was initiated to reveal if this d...
J Bjurman, C Herder


Laboratory and field trials of novel antisapstain formulations
1997 - IRG/WP 97-30146
This document covers the results of laboratory and field trials of combinations of fungicides formulated using a patented technology (PCT NZ 96/00143). A 3 week laboratory trial that uses radiata pine (Pinus radiata D. Don) branch discs as a substrate was used to determine which combinations possessed a suitable spectrum of fungitoxicity against key sapstain, mould and decay fungi. In particular f...
R N Wakeling, P N Maynard, D R Eden, C M Chittenden, B Carpenter


Xylophene AS™, the challenge of developing a modern antisapstain formulation for the french market
2000 - IRG/WP 00-30250
In France Sodium Pentachlorophenol (NaPCP) has been used for more than 40 years for the protection of freshly cut and unseasoned lumber. The French sawmill industry requires a protection of 6 months and maximum 10% surface infection by moulds and/or sapstain fungi. However, this active substance did have some serious environmental flaws and no authorisation was granted for a direct contact of NaPC...
E Wozniak, L Cubizolles, N Delourme Fonseca, A R Valcke, J Gors


Sapstain fungi associated with soft wood species in Kerala, India
1998 - IRG/WP 98-10260
Most of the packing case, plywood and match industries distributed throughout Kerala State, India utilise different timber species. The major problem in the utilisation of these species is their susceptibility to fungal sapstain and mould growth due to the conducive climatic conditions in the State. Several wood-based industries were surveyed and based on the economic importance and extent of saps...
E J M Florence, J K Sharma, R Gnanaharan


Evaluation of chlorothalonil for stain and mould control on lumber
1992 - IRG/WP 92-3713
Chlorothalonil is a commercially important fungicide with many industrial and agricultural applications. It has a very low mammalian toxicity and is considered to be an environmentally benign material. This paper describes the laboratory evaluation of chlorothalonil as a wood antisapstain treatment. Chlorothalonil was tested as three formulation types - an emulsifiable concentrate (EC), a water-ba...
P E Laks, T L Woods, D L Richter


Surface retentions of PCP, TCMTB and MTC obtained during a field trial of antisapstain formulations
1993 - IRG/WP 93-20002
Formulations containing NaPCP or TCMTB and MTC were included as reference products in a recent field trial of antisapstain formulations in Queensland. Retentions of these actives on the surface of treated sawn Pinus elliottii were monitored by ultrasonic solvent extraction of excised samples and analytical determination by high performance liquid chromatography. Distribution of actives with depth ...
D E Ferlazzo, M D Needham, M J Kennedy


Assessing the leachability of chlorothalonil and methylene bis thiocyanate from antisapstain treated radiata pine
1998 - IRG/WP 98-30176
Rain wash-off and leaching of antisapstain fungicides from wood may cause pollution of waterways and may also lead to a decrease in the efficacy of a formulation against fungal degrade as chemicals deplete from the timber surface. In the current study, the leachability of chlorothalonil (CTL) and methylene bis thiocyanate (MBT), the active ingredients of a commercial antisapstain formulation, was...
B Kreber, T L Woods


Characterisations of the mode of action(s) of Methylene bisthiocyanate (MBT) in Ophiostoma floccosum
2005 - IRG/WP 05-10554
Potassium (K+) leakage, oxygen consumption, glucose depletion and level of ATP formation were determined to elucidate the potential target site(s) of MBT on the sapstain fungus O. floccosum. Treatment of O. floccosum with MBT caused an efflux of intracellular K+ ions from the cells, and the rate of leakage increased with increasing MBT concentrations up to a certain value. This observation sugge...
T Singh, B Kreber, A Stewart, M Jaspers


Use of mixed populations of microflora to control sapstain on radiata pine
2002 - IRG/WP 02-10427
Most methods of biological control in the wood products field have focused on the use of single species of fungi or bacteria to control sapstain or decay. The approach taken in this study involved applying soil microorganisms, in combination with nutrients and various adjuvants that collectively form the biological control system. Radiata pine branch discs, autoclaved or fresh, were dipped in ...
C Chittenden, R Wakeling, B Kreber


Laboratory studies on control of sapstain and mold on unseasoned wood by bacteria
1991 - IRG/WP 1493
A malt-agar and nonagar laboratory test was used to evaluate the efficacy of bacterial preparations as biological control agents against several sapwood-inhabiting fungi. Both nonsterilized and filter sterilized bacteria preparations prevented attack by the sapwood-inhabiting fungi. Streptomyces rimosus was the most effective bacteria, giving good protection, with a 1-hr soak in a filter-sterilize...
T L Highley, R Benko, S C Croan


Effectiveness of some microbiocides against the development of molds and sapstain in Pinus elliottii
1981 - IRG/WP 3169
Eleven commercial biocidal formulations were tested in the field to determine their effectiveness in protecting logs and lumber of Pinus elliottii sapwood from sap stain and molds. Among these formulations 2-(thiocyanomethylthio) benzothiazole showed the best performance in controlling both types of fungi. Potassium N-hydroxymethyl-N-methyl dithiocarbamate and the mixture potassium N-methyl dithio...
S Milano


A field trial of water repellents as anti-sapstain treatment additives
1987 - IRG/WP 3417
The assessment of water repellents as anti-sapstain treatment additives has been included in a recent FRI research programme investigating improved surface protection of New Zealand Pinus radiata. Laboratory work has shown variation in water repellent effectiveness with chemical type and subsequently a field trial was established to examine the performance of selected water repellent/anti-sapstain...
J A Drysdale, D V Plackett


Comparison of the FRIM and forest research laboratory methods for screening of anti-sapstain formulations
1999 - IRG/WP 99-20170
Two laboratory methods for screening of anti-sapstain formulations were compared. The method adopted by the Forest Research Institute Malaysia (FRIM) involved 3 weeks assessment of rubberwood (Hevea brasiliensis) blocks, dipped in the candidate formulation and inoculated with mycelial/spore suspension of sapstain (Botryodiplodia theobromae) or decay (Schizophyllum commune) fungi, and incubated on ...
A H H Wong, D R Eden, C M Chittenden, M E Hedley, R N Wakeling


Growth of sapstain fungi in scots pine and the effect of timber ageing
1998 - IRG/WP 98-10269
A trial was initiated to study the growth of known isolates of sapstain fungi in scots pine (Pinus sylvestris) which had been naturally aged or sterilised by autoclaving or irradiation. Sawlogs cut from commercial thinnings were reduced to billet size and the exposed ends were painted with a bituminous sealant to reduce moisture loss. Some billets were inoculated after autoclaving or gamma-irradia...
N J Strong, J F Webber, R A Eaton


Field tests with Antiblue and Biocide TT for the prevention of sapstain and mould fungi on Pinus elliottii timber
1992 - IRG/WP 92-3682
Freshly cut and sawn Pinus elliottii timber was dipped in four concentrations of Antiblue (active ingredient sodium pentachlorophenate) and Biucide TT (local substitute), at four immersion times and stacked in the open at Chisengu sawmill. Five weeks later, the timber was assessed for the incidence and intensity of stain and mould. The results showed that Antiblue was the better chemical providing...
A J Masuka


Some studies on fungal deterioration of rubber wood (Hevea brasiliensis)
1980 - IRG/WP 2140
For the sreening of anti-stain chemicals trials with selected agricultural fungicides and new chemicals were carried out. Botryodiplodia theobromae, Aspergillus sp. and Penicillium sp. were used at test organisms. For testing the durability of rubber wood (Hevea brasiliensis) suitable local rotting fungi (Basidiomycetes), based on high degrading power, which may later be employed in standard tests...
A Sujan, A G Tan, M Stevens


Detoxification of methylene bisthiocyanate by bacteria isolated from freshly felled, anti-sapstain treated timber
1993 - IRG/WP 93-10017
The microbial defacement of freshly-felled timber treated with organic biocides continues to be a major problem in stored lumber. As part of a study to investigate the depletion in chemical activity of methylene-bis-thiocyanate (MBT) in green timber, bacteria were isolated from freshly-felled boards of Corsican pine (Pinus nigra var. maritima Arnold) previously treated with different biocides. The...
R J Wallace, R A Eaton, G R Williams


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