IRG Documents Database and Compendium


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Characterization of protein patterns from decayed wood of loblolly pine (Pinus taeda L.) by proteomic analysis
2008 - IRG/WP 08-10654
The primary biotic decomposers of wood belong to the basidiomycetes. The members of this group can attack and biodegrade both wood in the forest and in service. By the time wood decay is visible, there has already been a significant loss of strength. The identification of basidiomycetes and other organisms on wood only tell us what is present, not what is actively decaying the wood. When organisms...
Young-Min Kang, L Prewitt, S Diehl


Microbial Community Analysis Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis: Field Study Results
2008 - IRG/WP 08-20377
The effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on the fungal community in southern yellow pine (SYP) were assessed using terminal restriction fragment length polymorphism (T-RFLP) analysis. Field stakes treated with 0.25% and 0.37% ammoniacal copper quat (ACQ-C), 0.1 and 0.25% CTN, and 0.1 and 0.25% CTN combined with 2% BHT were installed with untreated controls in field s...
G T Kirker, S V Diehl, M L Prewitt, W J Diehl


Termites y otros insectos xiliofagos en bienes arquitectonicos monumentos nacionalos de Colombia
2009 - IRG/WP 08-10679
The incidence of termites and other wood destroying insects was surveyed in ten historical buildings in Colombia. The most common insects were termites (Kalotermes, Cryptotermes, Heterotermes), although numerous decay organisms were also detected, including beetles (Anobium, Lyctus, Cerambycids) and various wood decaying fungi. Structural wood elements were classified according to the degree of a...
M G Ramírez


Chemical Analysis in Production Quality Control at Wood Treatment Plants
2008 - IRG/WP 08-20396
Analysis methods for quality control analysis in wood treatment plants have evolved with the changes in treatment preservative chemistries and analytical instrument technology. The basic hydrometer specific gravity measurements used for solution strength and classic wet chemistry methods for wood have given way to instrumental techniques such as X-ray fluorescence, automatic titrator, and HPLC. No...
P Walcheski, L Jin


Durability and fungal colonisation patterns in wood samples after six years in soil contact evaluated with qPCR, microscopy, TGA, chitin- and ergosterol assays
2009 - IRG/WP 09-20402
There is a need to establish new objective and sensitive methods for early detection and quantification of decay fungi in wood materials. Molecular methods have proven to be a useful tool within wood protection issues, however, this field is still poorly explored and so far relatively few have used these methods within the field of wood deterioration. Among the techniques used in the indirect quan...
A Pilgård, G Alfredsen, I Børja, C Björdal


Assessment of mould growth on coated wood - methods and application
2009 - IRG/WP 09-20423
Discolouring fungi reduce the service life of coated wooden claddings in façades and increase the total cost of ownership due to shorter maintenance intervals. The project “Enhanced service life on coated wooden facades” has as its main objective to develop new methods for early prediction of durability and longer aesthetic service life of coated wooden cladding related to consumer needs and ...
L Ross Gobakken, C M Whist, O Høibø, P J Hovde, E Larnøy


Analysis on dynamics of basidiomycetes in decayed wood by a method using non-specific amplification of DNA
2009 - IRG/WP 09-20426
A combination of non-specific amplification of DNA by Phi29 DNA polymerase and specific amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) by polymerase chain reaction (PCR) was applied to elucidate the ratio of basidiomycete species inhabiting decayed wood from an outdoor bench. After more than 8 hours incubation with Phi29 DNA polymerase, concentration of DNA ...
T Wada, K Igarashi, M Samejima


Thermal behavior of zinc borate-treated wood
2009 - IRG/WP 09-30511
The objective of this study was to investigate the thermal behavior of zinc borate (ZnB)-treated solid wood by thermal analysis (TA). Thermogravimetry (TG) and differential thermogravimetry (DTG) curves obtained from TA showed that ZnB-treated wood specimens had higher char yields and lower maximum thermal decomposition temperature than untreated wood specimens. Reducing thermal decomposition temp...
E Terzi, H Sutcu, S N Kartal


Environmental Emission of Wood Preservatives: Interpretation of Data Relevant to BPD Risk Assessments
2009 - IRG/WP 09-50259
The risk assessments for the use of wood preservatives proposed by the OECD and used under the Biocidal Products Directive (BPD) (98/8/EC) require the derivation of leaching rates for active substances. These rates are to be used as input data in to agreed exposure scenarios. The leaching rates can be derived from laboratory testing and from field testing. The relationship between laboratory and f...
D G Cantrell


Life Cycle Assessment of treated timber: methodological aspects and first results
2009 - IRG/WP 09-50264
Environmental information on products is more and more required as a key to the market, and the building sector in Europe is certainly advanced in that direction. In France, Environmental Product Declarations are becoming very common for construction products. Therefore, the CTB P+ and CTB B+ certification comity as commissioned FCBA to perform the Life Cycle Analysis of treated timber in order to...
E Vial, M Bertrand, C Cornillier, G Deroubaix


Postia placenta gene expression during growth in furfurylated wood
2010 - IRG/WP 10-10734
Modified wood can provide protection against a range of wood deteriorating organisms. But we still lack information about why the modified wood is protected from microbial attack. Several hypotheses have been put forward for the mode of action against wood decaying fungi, including inhibition of action of specific enzymes, but they still need further testing. In this study gene expression of the b...
G Alfredsen, C G Fossdal


Towards Understanding the Biology of Wood Decay
2010 - IRG/WP 10-10739
Our previous research has focused primarily on ways to identify the wood decay fungi and microbial community. We continue to explore this complex and dynamic community and its interactions through microbial community ecology studies, gene expression interactions and proteomics. However, in order to better understand the mechanisms of fungal decay, we have sequenced the genome of a copper tolerant...
J Tang, K Jenkins, L Parker, S V Diehl


Stress wave and visual analysis of treated and non treated fence posts after 15 years in field test
2010 - IRG/WP 10-20449
Wood preservation may play an important role in protecting tropical rain forest. Basically, it can reduce pressure on natural resources by increasing wood durability. Field tests are conducted to simulate final use of woods under different environmental conditions. Visual analysis, non destructive testing, and non destructive evaluation techniques were applied to assess wood resistance and chemica...
A Florian da Costa, R Faustino Teles, J Costa Gonçalves


DNA-based tools for rapidly detecting, quantifying and monitoring ophiostomatoid fungi on beetles, in trees and wood products
2010 - IRG/WP 10-20450
Approximately half of the trees harvested for commercial purposes are lost because of native or introduced insects or insect-vectored microorganisms. Ophiostomatoid fungi, which are well adapted to dissemination by insects, include ~140 species of saprobes and pathogens. They are present worldwide, have high economical impact and many are subject to quarantine regulation. Thus, it is necessary to ...
L Khadempour, Young Woon Lim, S Massoumi Alamouti, C Breuil


Improved analysis of field test data related to service life prediction of tropical wood species
2010 - IRG/WP 10-20458
Long field trials of wood in ground contact give valuable data on the natural durability of the material. The European Standard EN 350 gives guidance on how to perform these durability classification, but is limited to the use of averages of in-service life of a set of specimens compared to a reference set. Starting from a database of visual assessment of field stake testing, it is possible to obt...
J Van den Bulcke, A Wong, Ling Wang Choon, Yoon Soo Kim, J Van Acker


Copper nanoparticles in southern pine wood treated with a micronised preservative: Can nanoparticles penetrate the cell walls of tracheids and ray parenchyma?
2010 - IRG/WP 10-30547
This study tests the hypothesis that copper nanoparticles can penetrate the cell walls of southern pine wood treated with a micronised preservative. We examined the nanodistribution of particles in tracheid and ray parenchyma cell walls using state-of-the-art HR (High Resolution)-TEM and HR-STEM (Scanning Transmission Electron Microscope)-EDX. These devices are capable of atomic-scale resolution. ...
H Matsunaga, Y Kataoka, M Kiguchi, P Evans


Analysis of phenolic extractives from various Tunisian species trees -Study of its potential valorisation
2010 - IRG/WP 10-30552
Phenol extractives are naturally synthesized compounds taken from several trees. It is well demonstrated that the presence of phenol compounds is positively correlated with the durability of wood. This property is very interesting for outdoor uses of wood with a limited treatment. Phenol compounds may also be used in the formulation of glue. In the aim to improve the knowledge on Mediterranean tr...
I Oueslati, F Charrier, K Seffah, A Moubarik, N Ayed, A Zerizer, B Charrier


Gene expression analysis of a copper-tolerant brown rot fungus on MCQ-treated wood
2011 - IRG/WP 11-10748
Most brown rot fungi are copper-tolerant, which makes them difficult to control with copper-based wood preservatives like MCQ. To better understand what biological processes are regulated, we used our model species, Antrodia radiculosa, to examine expression of genes on MCQ-treated wood. Our hypothesis was genes that decreased copper bioavailability would be up-regulated early, when wood showed ...
J D Tang, A Perkins, S V Diehl


Basidiomycete colonization of Scots pine sapwood quantified by qPCR and TGA
2011 - IRG/WP 11-10750
Scots pine (Pinus sylvestris L.) is an important softwood species in Northern Europe and is frequently used as material for various wood protection systems. In Europe, EN 113 is the standard basidiomycete laboratory durability test method, using mass loss as evaluation criteria. In this paper quantitative real-time PCR (qPCR) and thermogravimetric analysis (TGA) was used to characterize coloniza...
G Alfredsen, S Bollmus, T K Bader, K Hofstetter


The effects of acetylation level on the growth of Postia placenta
2011 - IRG/WP 11-10751
To understand the defence mechanisms utilized by decay fungi when exposed to different wood protection systems the study of gene expression can give us some answers. When the DNA sequences are known, primers can be designed to detect transcripts of genes with gene products related to basic cellular processes and hyphal growth. The characteristic gene products induced in different fungi by differen...
A Pilgård, G Alfredsen, C G Fossdal, C J Long II


Comparative investigations between thermal and microwave assisted digestion as a novel tool for the chemical analysis of inorganic wood preservative components
2011 - IRG/WP 11-20461
The correct chemical impregnation of wood requires an internal as well as external quality control of the treated wood. The active is located in a solid matrix, therefore it is often difficult to analyse such material since most analytical procedures are based on liquid systems. Because of this quite a number of methods – like extraction or thermal digestion - were developed in order to transfer...
H Ahl, J Fromm, E Melcher


Quantitative design guideline for wood outdoors above ground applications
2011 - IRG/WP 11-20465
This paper describes the background and principles behind an engineering design guideline for wood in outdoor above ground applications, i.e. use class 3 according to EN 335. The guideline has been developed in the European research project WoodExter and can be seen as a first prototype for a quantitative design tool in the area of wood durability. It is based on a defined limit state for onset of...
S Thelandersson, T Isaksson, E Suttie, E Frühwald, T Toratti, G Grüll, H Viitanen, J Jermer


Classification of wood materials using Fourier Transform Near Infrared Spectroscopy and multivariate analysis
2011 - IRG/WP 11-20471
A distinction between preservative-treated wood and non-treated wood can be demonstrated by the combination of Fourier Transform-Near Infrared Spectroscopy (FT-NIRS) and Multivariate Analysis (MVA). This technique is non-destructive, quick, easy to use and can be portable. The calibration models for different heavy metals are set up using principal component analysis (PCA) to classify species of t...
M A Bouslamti, M A Irle, C Belloncle, V Salvador, S Hulot, B Caron, E M Qannan


A comparative study and evaluation of methodologies used for determining wood preservative penetration
2011 - IRG/WP 11-20475
A series of methods for determining penetration of wood preservatives into the wood structure have been developed for either quality control (QC) or research and development purposes. QC methods range from monitoring the solution uptake, applying colour indicators or sampling specific wood samples followed by wood acid digestion/atomic absorption spectroscopy (AAS) or solvent extraction/chromatogr...
R Craciun, R Moeller, J Wittenzellner, T Jakob, J Habicht


Slice - Cut and Analyze Laser Microdissection (LMD): A method to produce sample material from modified wood for chemical quality analysis
2011 - IRG/WP 11-20478
Wood modification with its various systems is an established way to enhance a wood products’ service life in outdoor applications. Per definition, selected material properties are enhanced by means of modification without producing toxic substances or residues during service life and combustion afterwards. The systems of impregnation modification include a physical and often chemical alteration ...
K Zimmer, E Larnøy, A Treu, M Fongen


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