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An attempt to grow dry rot fungus Serpula lacrymans on mortar substrate in non-sterile conditions
1989 - IRG/WP 1385
According to many experts, Serpula lacrymans is probably the most dangerous wood destroying fungus in houses. Investigations of KOCH, A.P. (2) showed that incidence of this fungus in percent was around 20% when the frequency of some other standard fungi was far below (Coriolus versicolor: 0.2% for example). The work presented here is a part of a program carried out at CTFT for assessing the resist...
L N Trong


Differences in pH, electrical resistance, cation composition and NIR spectra of red spruce wood during early stages of brown rot degradation
2002 - IRG/WP 02-10449
Red spruce sapwood was exposed to degradation by the brown rot fungi Coniophora puteana, Postia placenta, Gloeophyllum trabeum and Serpula lacrymans for 0, 1, 2 or 3 weeks using a modified soil block assay design. Average weight losses over time ranged from 0-8.9% during this time period. Detectable changes in pH, electrical resistance and cation compostion were observed in the wood as early as 1 ...
J Jellison, S Kelley, B Goodell, D Hui, A Ostrofsky


Cytoplasmic and extracellular localization of manganese II dependent peroxidase(s) in white rot fungi during degradation of woody materials
1989 - IRG/WP 1416
The manner by which lignin is degraded in-situ in natural substrates by white rot fungi still remains a controversial issue particularly the distribution and role(s) played by lignin degrading enzymes (i.e. manganese II peroxidase and lignin peroxidase). In the present study, use was made of anti-manganese II peroxidase and immunolabelling techniques in conjunction with transmission electron micro...
G F Daniel, B Pettersson, T Nilsson, J Volc


Micromorphology of decay in Keruing heartwood by the basidiomycetes Phellinus contiguus and Dacrymyces stillatus
2002 - IRG/WP 02-10454
This study aimed to characterise the micromorphology of fungal decay in a wooden handrailing in an above ground exposure at the Thames embankment in London. Keruing heartwood (Dipterocarpus spp.) was determined as the construction timber and the two basidiomycetes Phellinus contiguus and Dacrymyces stillatus identified as main decay organisms. Their decay patterns within Keruing heartwood were...
G Kleist, M Ray, R J Murphy


European standardization for wood preservation
1991 - IRG/WP 2365
Since the last IRG 21 conference in New-Zealand, there one meeting of the plenary committee and several meetings of working groups. The interprative documents prepared by CCE for expliciting the essential requirements of the CCE Directive on the construction products and specially: mechanical resistance and stability / hygiene, health and environment / safety in use are waited to valid the progra...
Anonymous


Synergistic combination of an antioxidant and wood preservative: a preliminary study
1998 - IRG/WP 98-30172
We previously proposed that extractives in highly durable angiosperm heartwood may protect wood against white-rot fungal colonization and subsequent degradation by a dual mechanism: extractives have some fungicidal activity and are also free radical scavengers (antioxidants) and thus interfere with the fungal free radical degradative mechanisms. We tested this hypothesis using the commercial bioci...
T Schultz, D D Nicholas, J Minn, K D McMurtrey, T H Fisher


The high decay resistance in the sapwood of the naturally durable Malaysian hardwood Belian (Eusideroxylon zwageri)
2001 - IRG/WP 01-10410
It has long been assumed that the observed natural durability of the heartwood in certain timbers is perhaps associated with a relatively lower decay susceptibility also of the sapwood of these species. While the heartwood of Belian is reputedly highly decay resistant among the tropical hardwoods of Southeast Asia, laboratory decay tests reported in this paper have also confirmed the high decay re...
A A H Wong, A P Singh


Regulation of hyphal sheath formation iron-chelator production by the brown rot fungi Gloeophyllum trabeum and Postia placenta
1994 - IRG/WP 94-10074
Hyphal sheath formation and iron-chelator production by liquid cultures of the brown rot fungi Gloeophyllum trabeum and Postia placenta were monitored over time. The effects of incubation at various temperatures, pH's and concentrations of iron, manganese and nitrogen were determined. Hyphal sheath production was observed microscopically and quantified with JAVA video analysis. The hyphal...
Y Chen, J Jellison


The Effect on Biological and Moisture Resistance of Epichlorohydrin Chemically Modified Wood
2002 - IRG/WP 02-40224
Southern pine solid wood and fiber were chemically modified with epichlorohydrin to help in understanding the role of moisture in the mechanism of biological effectiveness of chemically modified wood. The solid wood had weight gains from 11% to 34%, while the fiber had weight gains from 9% to 75%. After modification, part of the specimens were water leached for 2 weeks or extracted for 2 hours ...
R E Ibach, B-G Lee


Microscopic analysis of southern pine utility pole core segments
1992 - IRG/WP 92-1542
Fifteen macroscopic core descriptive categories were evaluated by microscopic analysis for accuracy in detecting decay and judging decay severity. Five categories were good indicators of decay as decay was detected in 28 of 48 samples. Five categories were possible indicators of early decay as decay was detected in 6 of 40 core segments and an additional 18 contained hyphae. The remaining five cat...
S E Anagnost, C J K Wang, M Wright


The Copper Tolerance of Mycelium vs. Spores for Two Brown Rot Fungi
2002 - IRG/WP 02-10422
The copper tolerance of two brown rot fungi, Gloeophyllum sepiarium and Oligoporus placentus, has been tested using both mycelia and basidiospores as inoculum sources. Mycelial growth of O. placentus on agar medium was shown to be much more tolerant to copper (as copper sulfate) than that of G. sepiarium. However, there was no difference in copper tolerance of basidiospores between the two specie...
S Choi, J N R Ruddick, P I Morris


Suitability of cotton strip testing as a screening method for the development of wood preservative formulations
2003 - IRG/WP 03-20270
This paper outlines a simple and rapid test method for screening the efficacy of wood preservative formulations against microbiological attack using cotton strips. The method was evaluated against soft rot and was found to provide reliable information on the protection of lignocellulosic material against microbiological attack. The assessment is based on visual interpretation of decay and on a sim...
H Leithoff, I Stephan, H Härtner


Properties of cellulose degraded by the brown rot fungus, Postia placenta
1988 - IRG/WP 1350
To gain further understanding of the nature of the cellulose depolymerizing agent or agents of brown-rot fungi, brown-rotted cellulose was chemically and physically characterized. Various culture conditions, such as low nitrogen and elevated oxygen levels, did not induce degradation of cellulose by Postia placenta in liquid cultures. Therefore, brown-rotted cellulose for analysis was prepared by a...
T L Highley, R E Ibach, T K Kirk


Mechanistic aspects of the reactions of copper complexes with lignin in the presence of hydrogen peroxide and lipid hydroperoxide model compounds as proposed for white rot fungi
2001 - IRG/WP 01-10399
The structural principles and the oxidative reactions of the catalytic system consisting of copper, hydroperoxides, and copper coordination compounds that contain aromatic nitrogen atoms are similar to those of catalytic centers of oxidative enzymes involved in delignification reactions of white rot fungi. The system is capable of selectively degrading lignin and its low molecular weight would all...
K Fackler, P Lamaipis, E Srebotnik, M Humar, C Tavzes, F Pohleven, M Sentjurc, T Watanabe, K Messner


Developments in unsterile soil soft rot testing
1984 - IRG/WP 2210
Two unsterile soil test methods, the soil burial technique and a method using vermiculite burial with soil inoculation, were compared using different soils to determine their effectiveness in evaluation of wood preservatives against soft rot. Isolation of fungi and microscopy of decayed wood indicated that both methods produced accelerated soft rot attack in treated and untreated birch. Vermiculit...
P A McKaig


Fruitbody formation and basidiosporogenesis by the white rot fungus Pycnoporus cinnabarinus
1988 - IRG/WP 1348
Conditions for fruitbody formation by the white rot fungus Pycnoporus cinnabarinus in vitro were outlined. A marked difference in substrate requirements in comparison to previously tested brown rot fungi was revealed. Agar media containing Walseth cellulose and NH4 tartrate at 1 g/l permit profound production of basidiospores particularly at 15°C but only under light treatment. Higher NH4 tartrat...
J Bjurman


Collaborative soft rot tests: Tests conducted at Princes Risborough and Delft on soil samples from collaborating laboratories
1972 - IRG/WP 210
The laboratories which took part in the original collaborative soft rot test (Savory and Bravery, Holz u Org 5 (1) 58-90, 1970) sent samples of the soil used in the original test to Princes Risborough. Portions of this material were sent to Dr Hof for soil analyses at TNO and the remainder was employed at Princes Risborough for the conduct of comparative tests of soil virulence when used as a comb...
J G Savory


Extracellular hydrogen peroxide-producing and one-electron oxidation system of brown-rot fungi
1990 - IRG/WP 1445
Wood-component-degrading compounds involved in the initial degradation of the cellulose and lignin in wood were isolated from wood-containing culture of brown-rot fungi, Gloeophyllum trabeum and Tyromyces palustris and partially purified by gel filtration on Sephadex G-25 and DEAE-Sepharose ion-exchange chromatography. The compounds were glycoproteins. The molecular weights of the glycoproteins as...
A Enoki, S Yoshioka, H Tanaka, G Fuse


The nature of osmiophilic particles and their distribution during different stages of brown and white rot decay
1983 - IRG/WP 1213
The distribution of osmiophilic particles during the course of brown and white rot decay was investigated by applying transmission electron microscopic (TEM) methods. It was found that it correlates with the brown and white rot pattern.The osmiophilic particles are produced by the fungus and are supposed to be wood rotting enzymes....
K Messner, H Stachelberger


Growth of the copper tolerant brown rot fungus Antrodia vaillantii on different substrates
1995 - IRG/WP 95-10121
In recent years the copper tolerant brown rot fungus Antrodia vaillantii caused severe damages on impregnated wood in ground contact. The pattern of decay gave the impression that impregnated wood was even more severely attacked than unimpregnated. To investigate this question more closely laboratory tests were carried out. In a "choice test" Antrodia vaillantii grew preferably towards CC-impregna...
H Leithoff, I Stephan, M-T Lenz, R-D Peek


Targeted inhibition of wood decay fungi: degradation of cotton cellulose
1999 - IRG/WP 99-10321
Brown-rot decay is responsible for 80% of the damage and replacement of wood in service. For nearly thirty years, researchers have postulated a one-electron oxidase system combining Fe+2 oxalic acid, and H2O2 in the production of hydroxy radicals which ultimately oxidize cellulose in situ. Recently, researchers have been investigating antioxicants, in combination with biocides (Schultz, et al.,199...
F Green III, T A Kuster


Which substrate to use in a laboratory test with soft rot micro-fungi: vermiculite or unsterile soil?
1991 - IRG/WP 2372
A draft European Standard is being proposed by CEN TC 38 in which a vermiculite-burial and a soil-burial test to be carried out in succession are required. Various experiments with both procedures were set up with CCA, CC and propiconazole-formulations. The results show that vermiculite and unsterile soil yield comparable threshold values against soft rot. This poses the question of whether a two-...
A R Valcke


Influence of variable lignin content amongst hardwoods on soft-rot susceptibility and performance of CCA preservative
1982 - IRG/WP 1151
This paper presents the outline of an argument, based on both new and established data, which relates varying lignin content to variation of soft-rot susceptibility between hardwood species and of performance of CCA preservatives in controlling soft-rot. Although more data need to be generated to prove this relationship, sufficient evidence exists for the hypothesis to be given close attention, pa...
J A Butcher, T Nilsson


Effect of moisture and cellulosic substrate on decay of hardwoods by soft-rot fungi
1982 - IRG/WP 2173
The development by Forintek Canada Corp. of standard test procedures for laboratory preservative evaluations using soft-rot fungi was requested by the American Wood Preservers' Association. In Europe, through cooperative studies in the International Research Group on Wood Preservation, several attempts have been made to produce a standard procedure, but variability in results obtained bet...
A Byrne, R S Smith


Collaborative soft rot tests: Statistical treatment of results of soil analyses
1971 - IRG/WP 29
Institutes taking part in the collaborative experiments already reported forwarded samples of the soils employed in the tests to the Forest Products Research Laboratory, England. The soils were then autoclaved sterilised under uniform conditions and sent to the Department of Forest Soils, the Royal College of Forestry, Stockholm, Sweden. The results of the soil analyses are given in Table 1, toget...
A F Bravery


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