Your search resulted in 116 documents. Displaying 25 entries per page.
Application of DNA fingerprinting methods to identify biocontrol strains of fungi imperfecti
1994 - IRG/WP 94-10068
We have analyzed a number of biocontrol strains of Trichoderma harzianum and other Trichoderma strains with the methods DNA fingerprinting and PCR fingerprinting to differentiate and identify these strain which is not possible with morphological or biochemical methods. We could differentiate even gamma-ray induced mutants from each other as well as different strains form the same and different spe...
A Schlick, K Kuhls, W Meyer, E Lieckfeld, T Börner, K Messner
Characteristics of the pigments produced by sap-staining fungi
1997 - IRG/WP 97-10223
The present study was conducted to characterize the pigments produced by sap-staining fungi, Aureobasidium pullulans (de Bary) Arnaud and Ceratocystis piceae (Münch) H. and P. Sydow. The pigments isolated from tested fungi were identified as melanins by spectroscopic methods (UV and infrared spectra). The UV and the infrared spectra of the fungal pigments were similar to those of the synthetic me...
M Mori, M Takahashi
Molecular phylogeny of Ophiostoma spp. related to blue-stain in pine
2002 - IRG/WP 02-10432
Blue-stain in coniferous woods is an economically important disease in many temperate countries, caused specially by genus Ophiostoma. Classical taxonomy of genus Ophiostoma is limited for the correct identification of many species, because of the similar macroscopic and microscopic characters, as well as the existence of some species complex. Molecular identification is a good complement to eluci...
M Villarreal, F Arenal, J Sánchez-Ballesteros, M T De Troya, F Llinares, V Rubio, A Navarrete
Biological control of wood decay fungi. - Part II. Effects of exogenous nitrogen on effectiveness
2000 - IRG/WP 00-10360
The effects of nitrogen (urea and ammonium nitrate) on the ability of Trichoderma harzianum to inhibit the enzyme capabilities and decay capacities of Trametes versicolor and Gloeophyllum trabeum were investigated using a small wood sandwich decay test. The biocontrol fungus generally had a greater effect on the brown rot fungus, confirming previous reports. Nitrogen produced inconsistent effects ...
E A Canessa, J J Morrell
Antisapstain field trials of NeXgen in New Zealand
1997 - IRG/WP 97-30145
This document covers the results of summer and winter log trials of NeXgen, an antisapstain formulation containing methylene bis thiocyanate plus chlorothalonil. Log billets (20 - 25cm diameter) were stripped of bark using a commercial maul debarker and antisapstain treated within 8 hours of felling by dip application. Billets were stored in a pole barn and assessed for internal sapstain and decay...
R N Wakeling, T L Woods, D R Eden, C M Chittenden, B Carpenter
Analysis of volatile organic compounds (VOC's) from Trichoderma spp.: effect of media composition on VOC production and level of inhibition of wood decay fungi
1997 - IRG/WP 97-10207
Production of volatile organic compounds (VOC's) by two Trichoderma isolates grown on either malt extract or minimal media was examined and statistically assessed to identify which VOC's were predominantly produced on each of the two media types. The VOC's were captured onto chromatographic absorbent from above the cultures of the fungi before being analysed by integrate...
A Bruce, R E Wheatley, C Hackett, A Kundzewicz
Database of sapstain fungi affecting lumber, logs and trees
2004 - IRG/WP 04-10534
Sapstain fungi discolour lumber, logs and tree sapwood and are often mistaken for moulds, which cause a superficial discoloration. Stained wood has a lower market value. Further, because stained wood products can potentially carry pathogenic fungi, such products may be refused by importing countries. Addressing these issues involves developing ways for accurately identifying staining fungi, docume...
S Lee, F de Giuli Vallverdu, S Alamouti, Jae-Jin Kim, A Uzunovic, C Breuil
Enzymatic study of Ceratocystis sp., blue-stain fungi on Pinus nigra
1999 - IRG/WP 99-10315
One of the main problems that the forest exploitation industry has with Pinus nigra wood is the blue-stain fungi, whose causing agent is unknown. Therefore, the objective of this work has been to study, through enzymatic tests of the isolated cultures, if these fungi infect Pinus nigra in any specific way. After the incubations, isolates of Ceratocystis were obtained. These were cultured in a sali...
M T De Troya, F Rubio, D Muñoz-Mingarro, F Llinares, C Rodríguez-Borrajo, M Yuste, M J Pozuelo, J I Fernández-Golfín
Effects of chemical pretreatment of Douglas-fir heartwood on efficacy of potential bioprotection agents
1990 - IRG/WP 1440
Biological protection against wood decay fungi represents an environmentally attractive alternative to the use of chemicals; however, the process of identifying suitable candidates and the conditions that ensure successful protection pose major challenges. One critical aspect of this problem is to identify organisms that can rapidly and uniformly colonize the wood. Wood contains low levels of nutr...
B Dawson-Andoh, J J Morrell
Screening of bacteria, yeasts and Trichoderma isolates for antagonism toward stain and mould fungi on agar media and wood
1999 - IRG/WP 99-20159
A screening programme of soil bacteria for antagonism toward mould and wood stain fungi was undertaken on 8 types of agar-based media by application of several bacteria to each plate. The rapid screening method was subsequently verified by testing antagonism of the most promising bacteria on Scots pine sapwood. Most of the 64 bacterial showed antagonistic effects on certain media against individua...
C Payne, A Bruce
The role of chitinase in bioprotectant activity against staining fungi
1996 - IRG/WP 96-10175
Chitin is an important structural component of the hyphae of many wood staining fungi and its disruption can lead to dramatic declines in their growth. A number of bioprotectants have been shown to produce chitinases in liquid cultures, but the role of these enzymes in bioprotection remains poorly understood. The levels of these enzymes was studied by inoculating ponderosa pine sapwood wafers with...
J Liu, J J Morrell
Biological control of internal decay in Scots pine poles: A seven year experiment
1992 - IRG/WP 92-1529
The interactions between key fungi were monitored, by non-destructive and destructive sampling methods, during the course of an experiment on the effectiveness of Scytalidium sp. and Trichoderma spp. for eliminating established infections of Lentinus lepideus (Fr. ex Fr.) Fr. in creosoted Pinus sylvestris poles. Although extensively isolated at the beginning and end of the experiment, Trichoderma ...
P I Morris, D J Dickinson, B Calver
Preliminary agar screening studies as the first stage in the development of a biological control system against basidiomycetes in ground contact situations
1995 - IRG/WP 95-10111
Preliminary screening of Trichoderma isolates as possible biocontrol agents for protection of wood in ground contact situations against basidiomycete decay was undertaken on three types of media. Media used were 3% malt extract agar (MEA); a low nutrient synthetic media with a carbon: nitrogen ratio typical of that found in softwoods (LNM); and a similar synthetic media where the amino acid profil...
E J B Tucker, A Bruce
Growth inhibitory effects on blue-stain fungi of applied electricity fields
1996 - IRG/WP 96-10167
Exploratory laboratory experiments on the effects of electricity on two blue stain fungi Aureobasidium pullulans and Ceratocystis piceae on wood revealed that a potential gradient of 1 V/cm corresponding to a current of 15 mA (DC), applied without interruption during a 2 week experimental period, leads to an inhibition of the growth of these fungi. Germination is somewhat more sensitive than mycel...
J Bjurman
Biological control of sapwood-inhabiting fungi by living bacterial cells of Streptomyces rimosus as a bioprotectant
1992 - IRG/WP 92-1564
The objective of this study is to evaluate the efficacy of antifungal activity of living bacterial cells for the protection of wood against sapwood-inhabiting fungi. The following sapwood-inhabiting fungi were selected: sapstain --Ceratocystis coerulescens, Ceratocystis minor, Ceratocystis pilifera, and Aureobasidum pullulans; mold fungi --Aspergillus niger, Penicillium spp, and Trichoderma spp. L...
S C Croan, T L Highley
Degradation of resin constituents in various wood species by the white rot fungus Bjerkandera sp. strain BOS55
1999 - IRG/WP 99-10301
In previous studies, the white-rot fungus Bjerkandera sp. strain BOS55 was shown to cause extensive degradation of lipophilic extractives (resin) in Scots pine wood. Further research was carried out in order to investigate the ability of Bjerkandera sp. for reducing resinous constituents in various softwood (Douglas fir, larch and spruce) and hardwood species (birch, beech and poplar). The greates...
J Dorado, T A Van Beek, F W Claassen, R Sierra-Alvarez
Shorter-term biological control of wood decay in pre-seasoning pine roundwood as an alternative to chemical methods
1992 - IRG/WP 92-1555
Previous studies on the long-term control of decay in creosoted transmission poles, using Trichoderma and other antagonistic moulds, have met with limited success. However, it is possible biological control is more suited to control of decay on shorter time scales. An earlier study, focusing on pre-seasoning treatment of transmission poles showed that favourable porosity increases could be brought...
M W Schoeman, D J Dickinson
Assessment of the biocontrol potential of a Trichoderma viride isolate in a field trial
1998 - IRG/WP 98-10252
A field trial has been set up near Dundee, to assess the biological control potential of a Trichoderma viride isolate T60. This isolate has been shown in laboratory tests to be particularly effective in protecting wood against certain basidiomycete decay fungi.
Wood was treated with T60 spores using vacuum-pressure impregnation in a pilot preservation plant. Scots pine and Sitka spruce stakes wer...
H F Brown, A Bruce
The control and pretreatment decay in air seasoning Scots and Corsican pine poles in England
1990 - IRG/WP 1451
Previous work clearly showed that air-seasoned, British grown Scots and Corsican pine poles are subject to serious pretreatment decay. In order to control these infections a range of pretreatments are currently being investigated. One series of treatments is designed not only to control decay but also allow the development of mould and stain fungi in order to achieve the desired increases in poros...
D J Dickinson, A R Zahora, A P Dodson
Identification and inhibitory effect of volatiles from different ages of a Trichoderma aureoviride culture on selected wood decay fungi.
1995 - IRG/WP 95-10110
The ability of a Trichoderma sp. to produce volatile organic compounds (VOCs) over a four week period of growth was examined and the inhibitory effect of these volatiles against four selected basidiomycetes over the same period was assessed. After trapping, on tubes filled with chromatography packing material, VOCs were analysed on an integrated automated thermal desorbtion mass spectrometer syste...
A Bruce, A Kundzewicz, R E Wheatley
Comparative study of lignocellulolytic activities of Pleurotus spp. and white rot and brown-rot fungi
1997 - IRG/WP 97-10209
Field and fungal cellar trials have been set up to assess the biocontrol potential of a selected Trichoderma viride isolate in a situation representative of the end use of treated timber in ground contact situations. These trials are designed to give information about the efficacy of biological control as well as the suitability of existing chemical treatment methods for use with biocontrol fungi....
H L Brown, A Bruce
Biological control in termite management – the potential of nematodes and fungal pathogens
2004 - IRG/WP 04-10521
A brief overview on the options for biological control of termites is presented. Many organisms have been identified as being able to kill termites, however, we do not know their real impact on field populations of termites. Most research has focused on some entomopathogenic nematodes and the fungi Beauveria bassiana and Metarhizium anisopliae. To date, only a limited number of field studies have ...
M Lenz
The effect of chitosan on the growth and physiology of two wood inhabiting fungi
2006 - IRG/WP 06-10590
The effect of chitosan on the wood inhabiting fungi Sphaeropsis sapinea and Trichoderma harzianum was evaluated at cellular level. Increasing concentrations of chitosan caused an increase in the amount of peroxide in cultures of S. sapinea, which was accompanied by a simultaneous decrease in superoxide. The same effect was not observed in T. harzianum. The growth of both fungi was inhibited when h...
D Vesentini, T Singh
Quantification of methylene bis thiocyanate in wood and its effect on a sapstain fungus Ophiostoma floccosum
2006 - IRG/WP 06-10591
The mobility and concentrations of MBT at different depths of wood billets (200 mm long, 40 mm in diameter) were determined using NMR spectroscopy and ICP-ASE, and then correlated the effect of wood MBT concentrations on growth of O. floccosum in a bioassay using stereomicroscopy and confocal laser scanning microscopy (CLSM).
The 13C NMR spectra showed the presence or absence of MBT in wood but w...
T Singh
Efficacies of an insecticide and a fungicide for preventing blue-stain of Japanese red pine logs
2006 - IRG/WP 06-20329
We examined blue-stain fungi on Japanese red pine at 3 forests and 2 saw mills in Iwate Prefecture, Japan. The isolated ophiostomatoid fungi were Leptographium sp and Ophiostoma sp. There were 3 routes of the blue-stain; fungal growth from the spore or hypha stuck on the cross cut surfaces, fungal spread through the inner bark by bark beetles and fungal invasion around whorled knot. The larger ext...
H Taniuchi, T Koiwa, H Masuya, S Doi