IRG Documents Database and Compendium


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Prevention of sapstain in logs using water barriers
1997 - IRG/WP 97-30147
Placing logs in ponds or under water sprinklers to keep moisture levels high enough to prevent oxygen tensions rising above inhibitory levels, is a proven method of preventing sapstain. The objective of this experiment was to determine if water barriers significantly reduce the rate of sapstain penetration into logs by maintaining moisture contents, and consequently oxygen tensions, at inhibitory ...
R N Wakeling, D R Eden, C M Chittenden, B Carpenter, I Dorset, J Wakeman, R Kuluz


CCA removal from treated wood by chemical, mechanical and microbial processing
1998 - IRG/WP 98-50101-26
Most preservative-treated wood produced and consumed in the U.S. is treated with toxic inorganic compounds containing copper, chromium, and arsenic. Because chromated copper arsenate (CCA) is fixed to the wood, treated wood has not been considered toxic or hazardous and is currently landfilled. Increasing public concern about environmental contamination from treated wood combined with increasing q...
C A Clausen, R L Smith


Future Directions for Biological Control & BioActivity
2001 - IRG/WP 01-10416
Future directions for biological control of deleterious organisms on wood, and commercial market success, are dependent upon efficacy, cost and shelf life in comparison to other available means, and appropriate fulfilment of registration requirements. Basic and applied research involving ascomycete and/or basidiomycete fungi are described regarding solid wood applications of biocontrol of sapstain...
R L Farrell, J M Thwaites


Laboratory evaluation of anti-sapstain formulations
1989 - IRG/WP 3510
Four formulations were tested for their efficacy in controlling molds and sapstaining fungi on wood according to the Standard-II of Japan Wood Preserving Association (JWPA). Relative efficacy was compared on the basis of visual rating of the fungal growth on the treated and untreated wood surfaces when the wood specimens were exposed to each monoculture of 5 test fungi at 26±2°C and 70-80% for 4...
K Tsunoda


The effect of stack height on the performance of preservatives used for the prevention of sapstain on seasoning wood
1997 - IRG/WP 97-10192
The performance of three anti-sapstain preservatives was investigated under field conditions in south east England. The preservatives under test were: copper-8-quinolinolate (Mitrol PQ8) trimethylalkylammoniumchloride and sodium hexanoate (Sinesto B) and chlorothalonil (Tuff Brite). The preservatives were tested on Corsican Pine grown in south east England. The field trial was set up to investigat...
J R Williams, D J Dickinson, J F Webber


Comparison of bluestain fungi growing in vitro and in vivo
1996 - IRG/WP 96-10149
Both moulds and bluestain fungi cause serious economic losses for forestry and timber processing industries and much research is aimed at finding environmentally and economically acceptable methods of control. It is especially important to study the growth of these fungi in freshly cut wood, which has been unaltered by drying or sterilisation, and which therefore resembles the substratum they woul...
A Uzunovic, J F Webber, D J Dickinson


Protection of pine sawtimber and sawn timber against blue stain in Poland
1983 - IRG/WP 3193
The purpose of this paper is the synthesis of more important results of basic and applied research that have been grouped in the following problems: 1.: Fungi causing blue stain in the pine wood, occurring in Poland. 2.: Moisture content changes of pine sawtimber and sawn timber as the factor of blue stain producing fungi development. 3.: Effect of sapwood moisture content on the degree of wood st...
E Tarocinski, M H Zielinski


Sawmill Evaluation of a Bioprotectant against Moulds, Stain and Decay on Green Lumber
2003 - IRG/WP 03-10460
Moulds, stain and decay cause serious problems on wood utilization. Though a growing volume of wood is kiln-dried, the market for green exports remains significant, and environment-friendly wood protection is required to replace traditional chemicals. As a solution to this problem, Forintek Canada Corp. developed a bioprotectant for protecting logs and green lumber from moulds, stain and decay, wh...
Dian-Qing Yang, M Gignac, M-C Bisson


In vitro antifungal activity of chilli against wood degrading fungi
2006 - IRG/WP 06-10572
The efficacy of chilli juice and/or chilli extract oleoresin as antisapstain agents was evaluated against two common sapstain fungi, Sphaeropsis sapinea and Leptographium procerum. Possible synergy between chilli juice and Lactobacillus casei as antisapstain agents was also assessed. Both the chilli juice and the oleoresin showed moderate antifungal activity. No growth of the test fungi was obse...
T Singh, C Chittenden, D Vesentini


Quantification of methylene bis thiocyanate in wood and its effect on a sapstain fungus Ophiostoma floccosum
2006 - IRG/WP 06-10591
The mobility and concentrations of MBT at different depths of wood billets (200 mm long, 40 mm in diameter) were determined using NMR spectroscopy and ICP-ASE, and then correlated the effect of wood MBT concentrations on growth of O. floccosum in a bioassay using stereomicroscopy and confocal laser scanning microscopy (CLSM). The 13C NMR spectra showed the presence or absence of MBT in wood but w...
T Singh


Comparing microbial colonisation and Decay Rates of Wood from Sound and Aphid-Killed Kenyan-Grown Mexican Cypress (Cupressus lusitanica)
2006 - IRG/WP 06-10599
Samples of wood from 10, 15, and 30-year old trees attacked and killed by the cypress aphid (Cinara cupressi) and sound Kenyan-grown Cupressus lusitanica trees were investigated for variations in moisture content, density and susceptibility to microbial decay. MC varied with tree age, a normal trend, and between samples from aphid-killed and sound trees. In samples from 10, 15 and 20-year old so...
R Venkatasamy


Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP I: Accelerated laboratory decay study
2006 - IRG/WP 06-20332
The effects of Chlorothalonil (CTN) and Butylated Hydroxy Toluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and BHT is being evaluated for its synergistic effects with CTN. Results from an accelerated decay test will be presented. The...
G T Kirker, M L Prewitt, S V Diehl


The effect of woody and non woody plants extractives on microbial resistance of non-durable species
2006 - IRG/WP 06-30392
The effect of Elm (Zelkova carpinifolia), Oak (Quercus castanifolia), Mulberry (Morus alba), Ash (Fraxinus excelsior) as woody plants, Rose (Rosa damascene) and Fumitory (Fumaria sp) as non woody plants extractives on durability of Beech (Fagus orientalis), Maple (Acer insgin), Alder (Alnus subcordata), and Lime (Tilia sp) were studied. First wood species having extractives were cut to small piece...
S M Kazemi, A Hosinzadeh, M B Rezaii


Efficacy of chitosan in combination with GRAS (Generally Recognised As Safe) compounds as a potential wood preservative
2007 - IRG/WP 07-30414
In vitro studies were undertaken to evaluate the synergy between chitosan and selected GRAS (Generally Recognised As Safe) compounds against two sapstain fungi Sphaeropsis sapinea and Leptographium procerum. Bioassays performed on nutrient medium suggested that some of the GRAS compounds tested including sodium benzoate, potassium sorbate and ascorbic acid had a synergistic effect when combined w...
C Chittenden, K Thämelt, T Singh


Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP II: Results from field studies
2007 - IRG/WP 07-30429
The effects of Chlorothalonil (CTN) and Butylated Hydroxytoluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and the non-biocidal BHT was evaluated for its synergistic effects with CTN. ACQ-C was a positive control and untreated SYP sta...
G T Kirker, M L Prewitt, S V Diehl


Detection of Anti-Fungal Sapwood Extractives in Non-Durable Scots Pine (Pinus sylvestris), Rubberwood (Hevea brasiliensis) and Jelutong (Dyera costulata)
2007 - IRG/WP 07-10634
A general laboratory bioassay method of Woodward and Pearce (1985) was adopted to detect anti-fungal activity of sapwood or heartwood extractives of 5 Malaysian hardwoods [dark red meranti heartwood (Shorea spp.), red balau heartwood (Shorea spp.), kulim heartwood (Scorodocarpus borneensis), jelutong sapwood (Dyera costulata) and rubberwood sapwood (Hevea brasiliensis), including the temperate Pin...
A H H Wong, R B Pearce


In vitro evaluation of an integrated approach using Trichoderma harzianum and chitosan for the control of sapstain
2008 - IRG/WP 08-10659
In vitro assays were undertaken to evaluate the control of two sapstain fungi, Leptographium procerum and Sphaeropsis sapinea by a combination of chitosan or chitosan oligomer (CODP-14) and a naturally mutated strain of Trichoderma harzianum. Spore germination and hyphal growth of the test fungi were assessed on media amended with chitosan or chitosan oligomer with and without T. harzianum using ...
C Chittenden, T Singh


Biological control of the sapstain fungus Diplodia pinea (syn. Sphaeropsis sapinea) in the field
2008 - IRG/WP 08-10663
Diplodia pinea, syn. Sphaeropsis sapinea, is well known as the worldwide ocurring causal agent of the Diplodia tip-blight disease on various pine species, leading to great losses of valuable wood in the forests. Moreover, this fungus is also responsible for the fornation of sapstain of felled logs, leading to undesired disation of surface layers in the OSB manufacturing industry. In Central Europe...
C Gradinger, T Boisselet, K Messner, K Fackler


Microbial Community Analysis Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis: Field Study Results
2008 - IRG/WP 08-20377
The effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on the fungal community in southern yellow pine (SYP) were assessed using terminal restriction fragment length polymorphism (T-RFLP) analysis. Field stakes treated with 0.25% and 0.37% ammoniacal copper quat (ACQ-C), 0.1 and 0.25% CTN, and 0.1 and 0.25% CTN combined with 2% BHT were installed with untreated controls in field s...
G T Kirker, S V Diehl, M L Prewitt, W J Diehl


Investigation into the antifungal properties of herbal remedies for potential use in the wood preservation industry
2008 - IRG/WP 08-30462
The efficacy of herbal remedies as potential antifungal agents was evaluated against two sapstain and three decay fungi. Sapstain fungi included Sphaeropsis sapinea, and Leptographium procerum, and decay fungi were Oligoporus placenta, Coniophora puteana, and Schizophyllum commune. Out of six herbal remedies tested, three showed moderate to high antifungal activity. Caprylic acid had high antif...
D O’Callahan


Antifungal activity of essential oils against common wood degrading/decaying fungi
2008 - IRG/WP 08-30465
Despite the wide use of essential oils in pharmaceutical and food industry as antimicrobial agents, their use as wood preservatives has not been fully explored. In this study, 12 essential oils were screened in nutrient medium for their antifungal activity against 8 common mould, sapstain or decay fungi. Subsequently, one essential oil, eugenol was evaluated for decay resistance in an agar/wood ...
T Singh, C Chittenden


Microscopy evaluation of microbial decay patterns in wood stakes after 6 years in soil contact
2010 - IRG/WP 10-10715
Most studies on wood modification have so far been concerned with service life predictions rather than investigating the decay protection mechanism. Very little is known about the mode of action of these new treatments and about the strategies used by wood degraders in order to utilize the wood material despite a treatment. Access to such knowledge would allow further modification and improvement ...
A Pilgård, C G Björdal


Towards Understanding the Biology of Wood Decay
2010 - IRG/WP 10-10739
Our previous research has focused primarily on ways to identify the wood decay fungi and microbial community. We continue to explore this complex and dynamic community and its interactions through microbial community ecology studies, gene expression interactions and proteomics. However, in order to better understand the mechanisms of fungal decay, we have sequenced the genome of a copper tolerant...
J Tang, K Jenkins, L Parker, S V Diehl


Image analysis for mould and sapstain detection on wood
2013 - IRG/WP 13-20519
Laboratory tests for mould growth on wood products are time consuming and rely on visual assessments of specimens utilizing subjective rating systems. Accelerated laboratory methods are needed that can provide rapid, quantitative assessment of mould and sapstain growth on solid and composite wood products. Image analysis of scanned spore imprints from southern pine or oriented strandboard (OSB) sp...
C A Clausen, V W Yang


Microbial Community Analysis of Naturally Durable Wood in an Above Ground Field Test
2014 - IRG/WP 14-10826
This paper presents preliminary results of an above ground field test wherein eight naturally durable wood species were exposed concurrently at two sites in North America. Surface samples were taken at regular intervals from non-durable controls and compared to their more durable counterparts. Terminal Restriction Fragment Length Polymorphism was performed to characterize the microbial (bacteria, ...
G T Kirker, S V Diehl, P K Lebow


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