IRG Documents Database and Compendium


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Minimum inhibitory concentration of methylene bisthiocyanate towards selected wood inhabiting fungi in liquid culture and on radiata pine
2001 - IRG/WP 01-30261
The objective of the current study was to determine the minimum inhibitory concentration (MIC) of methylene bisthiocyanate (MBT) towards wood inhabiting fungi common on unseasoned radiata pine (Pinus radiata D. Don). The fungi used were Ophiostoma floccosum, Leptographium procerum, Sphaeropsis sapinea and Trichoderma viride. Fungi were individually inoculated into nutrient media spiked with differ...
T Singh, B Kreber, C Chittenden, R N Wakeling, A Stewart


Production, function and neutralization of oxalic acid produced by the dry rot fungus and other brown rot fungi
1987 - IRG/WP 1330
The formation of oxalic acid by the wood-destroying fungi causing brown rot, is found to be the key which by hydrolysing the hemicellulose brings the cellulose in the tracheid wall in contact with the cellulase enzymes and yeld watersoluble sugars leaving only a lignin skeleton. To control the pH in the substrate the excess oxalic acid is precipitated to water insoluble calcium oxalate by the dry ...
J Bech-Andersen


The durability of wood polymer composites against fungi and insects
2000 - IRG/WP 00-40161
New materials such as wood polymer composites are used more in France. These materials are obtained by mixing wood and thermoplastics in different ratios. These kind of material must be considered as new materials and some characteristics such as durability must be evaluated for outdoor applications. On the other hand, some recycled fibers from wood waste could be used as raw materials. Some woodp...
G Labat, I Le Bayon, J Gerard, F Amin


Development and Implementation of a DNA – RFLP Database for Wood Decay and Wood Associated Fungi
2004 - IRG/WP 04-10527
We are developing Restriction Fragment Length Polymorphism (RFLP) and Internal Transcribed Spacer (ITS) sequence databases for wood decay basidiomycetes and other fungi associated with wood. These databases currently house information for 39 fungal species consisting of 9 brown-rot basidiomycetes, 12 white rot basidiomycetes, 1 soft rot, 1 stain fungi, and 16 molds or other ascomycetes or imperfe...
S V Diehl, T C McElroy, M L Prewitt


Nondestructive assessment of biodegradation in southern pine sapwood exposed to attack by natural populations of decay fungi and subterranean termites
1994 - IRG/WP 94-20042
Field methods for evaluating decay resistance of experimentally treated materials lack a means for quantitative measurement of residual strength. Quantitative relationships between speed of impact-induced waves travelling parallel to the grain and residual compressive strength have been demonstrated in softwood attacked by brown rot-decay fungi, but the effects of termites have not been documented...
R C De Groot, R J Ross, W Nelson


Validation of the sapstain danger index
2000 - IRG/WP 00-10333
The Sapstain Danger Index (SDI) is a model that is used to indicate sites in the New Zealand forest where felled logs are more prone to sapstain, specifically it should indicate how soon after felling of a log should it be treated for sapstain. The validation of SDI has the goal to identify whether the SDI can be used to predict sapstain development and to eventually minimise loss of value of logs...
P A Cooper, S Downs, R l Farrell


Hydrolysis of bordered pits during colonization of conifers by brown-rot fungi
1995 - IRG/WP 95-10103
Brown-rot decay results in rapid reduction in degree of polymerization (DP) of holocellulose with concomitant strength loss (MOR) without removing lignin. Development of new methods of wood protection will require focusing on early events in the sequence of depolymerization. Bordered pit membranes (sapwood) represent a readily available source of non-lignified carbohydrate, ie. pectin and cellulos...
F Green III, J L Tschernitz, T A Kuster, T L Highley


The involvement of extracellular substances for the generation of hydroxyl radical during wood degradation by white-rot fungi
1997 - IRG/WP 97-10218
The activities of one-electron oxidation, hydroxyl radical generation, and phenol oxidase during the incubation of cultures of four white-rot fungi containing either glucose or wood were periodically measured. Further, their degradation activities against wood were examined during the course of cultivation. The generation of hydroxyl radical was correlated to the activity of wood degradation and i...
H Tanaka, S Itakura, A Enoki


Effects of the specimen position on fungal colonisation and wood decay by en 113 test fungi
1998 - IRG/WP 98-20136
For testing wood preservatives according to EN 113 it is common practice to plant the test blocks on neutral supports in order to prevent (1) a diffusion of chemicals into the agar medium and (2) an excessive moistening of the specimens. The procedure was employed in EN 350-1 for testing the natural durability of solid wood. It turned out to be of problematic nature because of the individual requ...
G Kleist, M-T Lenz, R-D Peek


Antagonistic properties of Gliocladium virens against wood attacking fungi
1996 - IRG/WP 96-10162
Gliocladium virens has shown good antagonism against decay fungi in agar medium and in wood blocks. Gliotoxin produced by Gliocladium virens is associated with biocontrol of some plant diseases, but its importance to biocontrol of wood-attacking fungi is unknown. We investigated the ability of gliotoxin-producing (GLT+) isolates of Gliocladium virens and gliotoxin-deficient (GLT-) mutants of Glioc...
T L Highley, H S Ananthapadmanabha, C R Howell


Biodetioration and strength reductions in preservative treated aspen waferboard
1983 - IRG/WP 2195
Experimental aspen waferboards, bonded with liquid or powdered phenol formaldehyde resins and treated by various methods with a wide selection of preservatives, were tested for fungal resistance in accelerated laboratory trials. Mold growth on the surface as well as weight and strength losses due to the actions of decay fungi were determined. Testing of board strength after decay in high and moder...
E L Schmidt, H J Hall, R O Gertjejansen, R C De Groot


Investigations on testing with soft rot fungi by the vermiculite-burial method
1972 - IRG/WP 215
With the object of developing a test method to determine the effectiveness of wood preservatives against soft rot attack, tests have been carried out with pure fungal cultures using vermiculite as the substrate for untreated and preservative impregnated wood blocks. In these tests the suitability of common commercial, simple disposable beakers was proved successful as a substitute for the glass bo...
W Kerner-Gang, M Gersonde


Screening of bacteria, yeasts and Trichoderma isolates for antagonism toward stain and mould fungi on agar media and wood
1999 - IRG/WP 99-20159
A screening programme of soil bacteria for antagonism toward mould and wood stain fungi was undertaken on 8 types of agar-based media by application of several bacteria to each plate. The rapid screening method was subsequently verified by testing antagonism of the most promising bacteria on Scots pine sapwood. Most of the 64 bacterial showed antagonistic effects on certain media against individua...
C Payne, A Bruce


Chemostimulatory and chemotropic responses by fungi to preserved and unpreserved wood
1981 - IRG/WP 1134
During experiments to determine the presence of biotic connections between soil and wood at this laboratory, marked chemotropic responses to wood were demonstrated by some wood destroying micro-organism in agar culture. These were shown at some distance from bait, up to 35 mm away, and on some occasions were unaffected by the presence of toxic materials. This paper provides preliminary data on the...
G Mowe, B King


The importance of blue stain attack for the colonization by wood-rotting fungi of wood not in contact with the ground
1988 - IRG/WP 1349
When used in constructions not in contact with the ground, wood has been shown often to proceed from blue stain to moulds. The appearance of wood rotting fungi is normally delayed. Solid wood artificially inoculated with the blue stain fungus Pullularia pullulans was shown to permit germination of Pycnoporus cinnabarinus basidiospores. This was demonstrated by the use of an indirect and a direct b...
J Bjurman


Evaluation of white-rot fungal growth on Southern Yellow pine wood chips pretreated with blue-stain fungi
2000 - IRG/WP 00-10349
White-rotting basidiomycetes do not colonize on southern yellow pine. This study seeks to reduce the resinous extractive content of southern yellow pine by treating it with blue stain fungi. The mycelial growth of wood-inhabiting ligninolytic white-rot fungi can be achieved on pretreated southern yellow pine wood. Aureobasidium, Ceratocystis, and Ophiostoma spp. removed 70% to 100% of the extracti...
S C Croan


Laboratory trials of biological control agents against subterranean termites
1991 - IRG/WP 1475
To screen for potential termite control by microorganisms, 17 mold fungi included 3 pathogenic fungi and 7 basidiomycetes, were used to challenge the test termite species, Coptotermes formosanus and Reticulitermes speratus. Test fungi were allowed grow and completely in Petri dishes. To screen for potential termite control by microorganisms, 17 mold fungi included 3 pathogenic fungi and 7 basidiom...
K Suzuki


Northern oak wood and its damages in Iran
1988 - IRG/WP 1377
The Northern oak species of Iran designated Quercus castaneaefolia C.A.M. is distributed widely in caspian littoral. In natural conditions (healthy) has favourable quality and it is used mostly in rural construtions, sleepers, parquets, cross arms for electric and communication poles, it is used also indoor and window construction, benches and finally veneer. This wood in the case of sleepers and ...
D Parsapajouh, P Niloufari


Variation in biological performance of CCA caised by preservative application method
1996 - IRG/WP 96-40072
A series of laboratory studies to investigate the influence of treatment application method on CCA performance in Cosican pine has been completed. Biological decay tests, such as serial exposures, were used to induce decay in wood at preservative retentions of up to 10 kg/m³ CCA salts. Significant differences in performance of the preservative against either brown, white or soft rot decay fungi w...
P R Newman, R J Murphy


Physiological properties of fungal test strains according to the European Standard EN 113
1986 - IRG/WP 2258
For the discussion of the European standard EN 113 the EMPA's procedure of culturing the test fungi and the corresponding virulence of the test fungi as well as the wood moisture content at the end of the test are shown. It is mainly shown that within the standard the choice of the solvent may not be left at the test lab if reproducible results shall be obtained. The different solvents in...
E Graf, B Zgraggen, P Manser


Isolation and identification of the fungal flora in treated wood. Revised technique
1977 - IRG/WP 159
At the 8th Annual Meeting in Wildhaus a paper was presented for discussion on the isolation of fungi from treated wood.·Since then work of this nature has been undertaken at Imperial College and as a result a revision of that document has been made and is presented here. The main alterations are: 1) To streamline the isolation procedure 2) Modification of the benomyl agar 3) The inclusion of a st...
C P Clubbe, J F Levy


Influence of the decay of spruce chips by the selected fungi on their chemical structure and the pulp properties
2004 - IRG/WP 04-10510
The paper focuses on changes in the weight and in the molecular structure of spruce chips submitted to long-term storing as well as to medium- and long-term model rotting degradation caused by some chosen fungi identified at storing processes on the pile. For the model decay of chips under laboratory conditions during 3 or 6 weeks the white-rot fungi: Phanerochaete chrysosporium, Heterobasidion an...
R Solár, L Reinprecht, A Geffert, F Kacík


Evaluation of tropolone as a wood preservative : activity and mode of action
2002 - IRG/WP 02-30282
The fungicidal activity of 2-hydroxy cyclohepta-2,4,6-trienone (tropolone) analogue of b-thujaplicin a natural product responsible for the durability of heart wood of several cupressaceous trees was investigated in vitro on growth of white and brown rot fungi. Results obtained show that tropolone, easily prepared from commercially available products, possesses strong fungicidal activity similar ...
P Gérardin, M Baya, N Delbarre, P N Diouf, D Perrin, P Soulounganga, E Gelhaye, J P Jacquot, C Rapin


The role of chitinase in bioprotectant activity against staining fungi
1996 - IRG/WP 96-10175
Chitin is an important structural component of the hyphae of many wood staining fungi and its disruption can lead to dramatic declines in their growth. A number of bioprotectants have been shown to produce chitinases in liquid cultures, but the role of these enzymes in bioprotection remains poorly understood. The levels of these enzymes was studied by inoculating ponderosa pine sapwood wafers with...
J Liu, J J Morrell


Accidental mold/termite testing of high density fiberboard (HDF) treated with borates and N’N-naphthaloylhydroxylamine (NHA)
2003 - IRG/WP 03-10462
High density fibreboard (HDF) was made from beech and pine furnish (50:50) and treated with boric acid (0.1-3%), borax (0.1-3%) or N'-N-(1,8-naphthalyl) hydroxylamine (NHA) (0.1-1%) prior to gluing with urea formaldehyde (UF) resin in order to determine resistance to Eastern subterranean termites (Reticulitermes flavipes Kollar), the most economically important termite species in North Am...
S N Kartal, H H Burdsall Jr, F Green III


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