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Characterization of tropical wood-decaying fungi by RFLP analysis of PCR amplified rDNA
1998 - IRG/WP 98-10251
Five brown-rot fungi (Tyromyces palustris, Gloeophyllum trabeum, Antrodia sp., Poria placenta, Coniophora puteana) and three white-rot fungi (Coriolus versicolor, Pycnoporus sanguineus, Lentinus squarrosulus) registred as representative wood-decaying fungi for international standards of wood durability tests have been characterized using molecular tools (PCR/RFLP). The Internal Transcribed Spacer ...
A Zaremski, M Ducousso, Y Prin, D Fouquet


Soft rot studies on CCA treated eucalypt power transmission poles
1981 - IRG/WP 1132
Initial results found for sixty CCA treated eucalypt poles, from a soft-rot survey are discussed. Retentions of CCA within above and below ground pole samples were variable. The variation was attributed mostly to biodegradation and effect of soil environment, rather than leaching. Bacterial decay was found in many poles and was severe in some cases. Although found in the presence of soft-rot decay...
L E Leightley


Wood decay of Pinus sylvestris L. by marine fungi
1990 - IRG/WP 1463
Waterlogged wood decay by marine microorganisms represents a major problem for the conservation of wood structures of historic value. It is important to know which kind of decay affects the wood from a conservation point of view. Enzyme activities of 14 marine fungi belonging to several localities were studied. Also, the weight loss and the rot type suffered by the wood at a microscopic level were...
M C Escorial, M T De Troya, J E Garcia de los Rios


The effect of malt and agar trademarks on growth, decay and stilbene resistance of fungi
2000 - IRG/WP 00-20202
Effects of malt and agar trademarks used in growth media on growth of fungi in cultures, amount of decay they cause in birch samples and their resistance to stilbenes were tested. It was tested also had the conditions where fungus cultures were stored before inoculation of test plates some effect on the results. Fungi used in these tests were white rot fungus Trametes versicolor and brown rot fung...
L Syrjälä, L Paajanen, A Pappinen


Treatment of wood with formaldehyde. Acid catalysis of the reaction between formaldehyde and wood
1980 - IRG/WP 3146
Formaldehyde reacts with the free OH-groups in wood forming cross-linking bonds. The reaction can be brought about without any catalyst but in that case the activation energy is very high and high temperatures and long reaction times are needed. Normally, the reaction is catalysed by different acids. Acid catalysts, however, tend to degrade the cellulose and the wood matrix causing reduction in th...
T Vihavainen, K Piispanen, P Mansikkamäki


UV-microspectrophotometry of lignin and accessory compounds in wood cell walls of conifers
1999 - IRG/WP 99-20171
The distribution of lignin in the cell wall of conifer tracheids was determined by UV-VIS microspectro-photometer. Wood sections of one micron thickness were measured between the wavelength 240 and 700 nm. Spectral analyses revealed that the conifer lignin associated with the tracheid secondary wall and middle lamella is composed of guiacylpropane units which exhibit a characteristic UV absorption...
G Kleist, G Koch, J Bauch


Remediation of pentachlorophenol- and creosote-contaminated soils using wood-degrading fungi
1994 - IRG/WP 94-50021
Microbiological treatment of hazardous wastes has generally been associated with the use of bacteria. During the past decade a significant body of evidence has accumulated that demonstrates that fungi, in particular white-rot fungi, have the ability to degrade a wide range of hazardous organic compounds (xenobiotics) and thus might also be useful for treatment of materials contaminated with these ...
R T Lamar, T K Kirk


The colonization of selected naturally durable timbers by marine fungi and borers
1977 - IRG/WP 439
In recent years, concrete and metal have been widely substituted for wood in contact with sea water, but wood products have not lost their usefulness under such conditions. In many cases, wood if sound and durable, may prove to be the most practical and economical of materials used in sea water exposure. Timber when immersed in the sea may be attacked by micro-organisms (bacteria and fungi) and ma...
S E J Furtado, E B G Jones


CCA modifications and their effect on soft rot in hardwoods. Part 2
1983 - IRG/WP 3244
The work outlined in this document is a continuation of that presented in Document No: IRG/WP/3201. The findings described in the previous paper are summarised below: a double treatment of CCB followed by arsenic (CCB+A) is more effective than a double treatment of boron followed by CCA (B+CCA) or a single treatment of CCA, CCB or CCAB in controlling soft-rot due to Chaetomium globosum in birch. C...
S M Gray, D J Dickinson


Valid names for some common decay fungi and their synonyms
1992 - IRG/WP 92-1522
The taxonomy of wood destroying fungi has changed noticeably within the last ten years. New genera and more well defined generic concepts have emerged with concomitant and necessary changes in nomenclature. The following is a revision of a list originally prepared by Kaarik (1979) in collaboration with Professor Dr. C. Jacquiot (France), Mr. J. G. Savory (United Kingdom) and Dr. G. Seehann (Federa...
M J Larsen, R M Rentmeester


Fungi used in standard tests on the toxicity value of wood preservatives in various European countries
1975 - IRG/WP 255
The aim of the present paper is to make the comparative analysis of test fungi used in various European countries in order to define the toxicity value of wood preservatives against fungi of the Basidiomycetes class. Only the methods with national standard rank, present on the currently binding standards list are taken for consideration. The analysis of similarities and differences in the choice o...
J Wazny


Termite and decay resistance of particleboard composed of white cypress pine and radiata pine
1997 - IRG/WP 97-10200
Phenol-formaldehyde bonded particleboard was manufactured from blends of non-durable radiate pine (P. radiate) and heartwood of the naturally durable species, white cypress pine (C. glaucophylla). Board specimens were subjected to bioassays using two termite species, M. darwiniensis and C. acinaciformis, and the basidiomycete fungi, C. puteana and P. ostreatus, and the durability of specimens was ...
P D Evans, J W Creffield, J S G Conroy, S C Barry


Attempt for developing a new method for above ground field testing of wood durability
2000 - IRG/WP 00-20199
Field testing remains the most appropriate way for evaluation of wood preservatives or natural durability of wood species. Above ground tests are designed to answer specific questions concerning the outdoor utilisation of wood. Such methods supply additional information and confirm or reject preliminary laboratory results. The European standard (ENV 12037, "lap-joint") for assessment of the durabi...
N Terziev, M-L Edlund


Reaction of osmium tetroxide with a diphenolic compound as a model substance for demethylated lignin
1986 - IRG/WP 1303
By using caffeic acid as a model substance for diphenolic moieties of demethylated lignin it was found that electron dense particles called by the authors "osmiophilic particles" in former papers are reaction products of OsO4 with the model substance. By these products it is possible to indicate the enzymatic demethylation of lignin taking place during wood decay. The appearance of the electron de...
K Messner, E Srebotnik


Susceptibility of CCB treated wood to fungal colonization
2003 - IRG/WP 03-10492
CCB treated wood is generally resistant to all wood decay fungi. However, like CCA impregnated wood, susceptibility of CCB treated wood to copper tolerant fungi have been observed. The ability of various brown rot fungal hyphae to penetrate and overgrow the wood samples was investigated. Samples made of Norway spruce (Picea abies) were impregnated with 5 % CCB solution according to the EN 113 proc...
F Pohleven, U Andoljsek, P Karabegovic, C Tavzes, S A Amartey, M Humar


Preventive effectiveness of petroleum derivatives against blue-stain fungi. Part 2
1993 - IRG/WP 93-30001
Solvex-Tar, a product derived from the distillation of petroleum could be suitable for the treatment against blue-stain fungi in wood used in exterior carpentry, due to its effectiveness as a fungicide. The aim of this work has been to optimize the application of this product, modifying it as necessary, so as to improve its physical characteristics without interfering with its preventive effective...
E Sanchez, M T De Troya, A M Navarrete, A Guijarro


Evaluation of fungal remediation of creosote treated wood
1998 - IRG/WP 98-50101-25
Biotechnological remediation of creosote treated wood may be of interest in connection with novel recycling processes. White rot fungi and/or their ligninolytic enzyme systems are supposed to be valuable tools for such processes. This paper reports about results achieved when creosoted wood was treated in solid substrate fermentation with selected white rot fungi after different extraction procedu...
K Messner, S Böhmer


The efficacy of Sentry® as a treatment for the control of sapstain in pre-infected radiata pine
2001 - IRG/WP 01-30260
Experiments were conducted to determine the efficacy of Sentry® when used to control sapstain fungi on radiata pine branch billets stored under high fungal hazard conditions. Prior to antisapstain treatment, the billets were pre-infected with a mixed inoculum of staining fungi and held from zero to 10 days at 25°C and 75% relative humidity. This was done to produce billets with different degrees...
C Chittenden, J G van der Waals, B Kreber, R N Wakeling


Comparative performance of several ammoniacal copper preservative systems
1997 - IRG/WP 97-30151
The efficacy of several ammoniacal copper-based wood preservative systems was evaluated in this study. The selection of potential co-biocides was based on the results of an agar plate test. Following this, the most promising systems were evaluated in a standard field stake test. Good correlation was found between the agar plate and field stake test results. Of the preservative systems tested, copp...
D D Nicholas, T Schultz


The effect of tunicamycin on production and secretion of extracellular carbohydrate-degrading enzymes by Postia placenta
1988 - IRG/WP 1342
The extracellular carbohydrate-degrading enzymes of wood-decay fungi are usually heavily glycosylated and therefore stable under most denaturing conditions. It is unlikely that wood decay can be prevented by simply inactivating these enzymes. Tunicamycin, an antibiotic produced by Streptomyces lysosuperificus, prevents the glycosylation of glycoproteins and can interfere with the secretion of thes...
J A Micales, T L Highley


Using DNA probes to characterize the metabolic pathway of pigment production in several wood-staining fungi
1996 - IRG/WP 96-10146
During shipment and storage, lumber is susceptible to sapstain, a wood discoloration caused by fungi. Currently kiln drying and chemical applications are used to control sapstain. However, the chemicals used to protect wood have a broad range of action, and so can affect other organisms. In addition, in Canada most of these chemicals are under temporary registration. Thus there is a need to develo...
R Eagen, S Riecken, J Kronstad, C Breuil


In vitro sporulation of selected wood decay fungi
1978 - IRG/WP 190
Basidiospores produced axenically without laborious attention in the laboratory are useful in studies of wood decay initiation. Such spores presumably approach those collected from natural sporophores in size and germinability (Morton, 1964). Production of spores in vitro by inversion of cultures grown on 2% malt extract agar in deep glass dishes (100x80 mm²) has been the preferred method (Morton...
E L Schmidt, D W French


Wood extractives as carbon sources for staining fungi in the sapwood of lodgepole pine and trembling aspen
1995 - IRG/WP 95-10098
Wood discoloration by sapstaining fungi reduces lumber value. In Canada, the most commonly recorded sapstaining fungi belong to the genera Ophiostoma. This work evaluated the effect that Ophiostoma piceae, Ophiostoma ainoae, and Ophiostoma piliferum used the lipids present in the sapwood of lodgepole pine (Pinus contorta Dougl.) and trembling aspen (Populus tremuloides Michx.). Wood lipids, common...
Yong Gao, C Breuil


Étude in vitro de la colonisation et de la dégradation structurale du bois d'aubier de Pin sylvestre par la Mérule: Serpula lacrymans Schum. ex Fr. S. F. Gray
1979 - IRG/WP 198
The degradation of Scots pine sapwood cell walls by Serpula lacrymans, a brown rot fungus, is observed after various periods of exposure from two weeks to twelve weeks. The observation by microscopy shows that the hyphae of Serpula rapidly invade the wood tissues as cell wall degradation starts. That deterioration is not gradual, it is observed to be very irregular as well within the whole of the ...
D Dirol


Moisture requirements and wood degradation of pine and spruce wood by some Basidiomycetes-fungi
1989 - IRG/WP 1406
The minimum moisture requirements for the growth of the brown rot fungi, Coniophora puteana, Gloeophyllum trabeum and Serpula lacrymans were near or above the fibre saturation point of pine and spruce, sap and heartwood, during 160 days incubation according to the standard method EN 113. The moisture of wood for the optimum growth of Coniophora puteana and Serpula lacrymans was about 35-80% and fo...
H Viitanen, A-C Ritschkoff


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