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The role of oxygen and oxygen radicals in one-electron oxidation reactions mediated by low-molecular weight chelators isolated from Gloeophyllum trabeum.markup
1994 - IRG/WP 94-10086
The KTBA assay for determination of one-electron oxidation activity was used to assay reactions of low-molecular weight chelators isolated from the brown rot fungus Gloeophyllum trabeum. The assay, performed either under air or nitrogen showed that molecular oxygen was an important factor in chelator-mediated oxidation reactions. A reduction in oxidative activity was observed when superoxide dismu...
Jun Lu, B Goodell, Jiang Liu, A Enoki, J Jellison, H Tanaka, F Fekete


Hydrolysis of bordered pits during colonization of conifers by brown-rot fungi
1995 - IRG/WP 95-10103
Brown-rot decay results in rapid reduction in degree of polymerization (DP) of holocellulose with concomitant strength loss (MOR) without removing lignin. Development of new methods of wood protection will require focusing on early events in the sequence of depolymerization. Bordered pit membranes (sapwood) represent a readily available source of non-lignified carbohydrate, ie. pectin and cellulos...
F Green III, J L Tschernitz, T A Kuster, T L Highley


Antagonistic properties of Gliocladium virens against wood attacking fungi
1996 - IRG/WP 96-10162
Gliocladium virens has shown good antagonism against decay fungi in agar medium and in wood blocks. Gliotoxin produced by Gliocladium virens is associated with biocontrol of some plant diseases, but its importance to biocontrol of wood-attacking fungi is unknown. We investigated the ability of gliotoxin-producing (GLT+) isolates of Gliocladium virens and gliotoxin-deficient (GLT-) mutants of Glioc...
T L Highley, H S Ananthapadmanabha, C R Howell


Micromorphology of oak wood degraded by brown rot fungus Coniophora puteana
2000 - IRG/WP 00-10356
To characterize the degradation of hardwood by brown rot fungus, Coniophora puteana was incubated on the wood blocks of Quercus accutissima. For comparison, the same fungus was also incubated on the pine wood blocks of Pinus densiflora. In softwood, the shape of the wood cells and thickness of the cell appeared unchanged even the loss of birefringence in the affected areas. In contrast, oak wood d...
Yoon Soo Kim, Seung-Gon Wi, Kwang-Ho Lee


Oxalic acid metabolism of Postia placenta
1992 - IRG/WP 92-1566
The oxalic acid metabolism of Postia placenta was studied in liquid culture in order to identify the metabolic pathways of biosynthesis and to determine whether oxalic acid physiology could be correlated with efficiency in wood decay. Oxalic acid production was determined for test strain MAD698 grown in a basal defined medium with a variety of different carbohydrate and nitrogen sources. The highe...
J A Micales


Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips, immunolabeled with or without prior fixation, and examined by SEM. Enzymes were localized on the hyphal surface and on the clumped fibrillar ...
F Green III, C A Clausen, M J Larsen, T L Highley


The effect of Tween 80 on the growth, morphology, and enzyme secretion of Postia placenta
1990 - IRG/WP 1456
The nonionic surfactant Tween 80 (polyethylene oxide sorbitan mono-oleate) has been reported to increase enzyme production and/or secretion in bacteria and fungi. Such a procedure could greatly facilitate research into the physiology of wood-decay fungi since quantities of available enzyme are often limiting. The brown-rot fungus Postia placenta was grown in a synthetic medium supplemented with 0,...
J A Micales


Changes in some important mechanical and physical properties of untreated and treated mining timber after exposure to the conditions of a colliery
1996 - IRG/WP 96-20082
Each of salt treated and untreated mining timber (wedge) pairs with 5x15 cm² cross-section and 155 cm length prepared from Scots pine, Bornmüller's fir and oriental beech was cut in the small standard test specimens to determine changes in some important mechanical and physical properties, 13 month after exposure to the conditions of Kozlu Hard Coal Mines in the depth of 52 m. Thus, the...
A Bobat


Preliminary investigation on the natural durability of Guayule (Parthenium argentatum)-based wood products
2000 - IRG/WP 00-40154
Conventional preservatives used to protect wood from insect and microbial damages are presently of major concern to human health and the environment. Finding alternative and economical preservatives has not been successful. Previous studies have shown that the resinous material extracted from the guayule plant (Parthenium argentatum, Gray) has both insect- and microbial-resistant properties. Unfor...
F S Nakayama, P Chow, D S Bajwa, J A Youngquist, J H Muehl, A M Krzysik


Effect of remediation on the release of copper, chromium, and arsenic from particleboard made from CCA treated wood
2001 - IRG/WP 01-50170
This study sought to determine the effect of remediation with oxalic acid (OA) extraction and Bacillus licheniformis fermentation on the release of copper, chromium, and arsenic from particleboard made from remediated wood particles and also investigates durability of the particleboard against white and brown- rot fungi. Particleboard samples were manufactured using untreated, CCA-treated, OA-extr...
S N Kartal, C A Clausen


Defining fungal decay types - A proposal for discussion
1985 - IRG/WP 1264
The present definitions of the fungal decay types, white rot, brown rot, and soft rot are somewhat vague. The definitions are based on a mixture of criteria involving morphological and chemical aspects of decay as well as taxonomic affiliation of the responsible fungal species. The present vague definitions have led some fungi being erroneously places in a wrong decay type. Furthermore, some fungi...
T Nilsson


Neem leaves, a potential source for protection of hardwood against wood decaying fungus
2005 - IRG/WP 05-30370
Neem leaves are widely recognized for their insecticidal, pesticidal, fungistatic and fungitoxic ability. The present study is to investigate the efficacy of neem leaves extractives against wood destroying microbes, a brown and a white rot. Neem leaves extractives prepared in various solvents such as petroleum ether, acetone, ethanol, methanol and hot water were tested at a concentration levels of...
S Dhyani, S Tripathi, V K Jain


The action of siderophores isolated from Gloeophyllum trabeum on the structure and crystallinity of cellulose compounds
1991 - IRG/WP 1479
Low molecular weight, high affinity iron-binding compounds (siderophores) were isolated from the brown-rot fungus Gloeophyllum trabeum. The compounds were shown to be inducible by iron starvation and could be purified by ultra-filtration, ethyl acetate extraction, column chromatography and preparative HPLC. The isolated compounds were shown by analytical and immunological techniques to be produced...
J Jellison, V Chandhoke, B Goodell, F Fekete, N Hayashi, M Ishihara, K Yamamoto


A model for attack at a distance from the hyphae based on studies with the brown rot Coniophora puteana
1995 - IRG/WP 95-10104
In timber infested by brown rot fungi, a rapid loss in strength is attributed to production of hydroxyl radicals (HO·) at a distance from the hyphae. The immediate precursor is Fenton's reagent (Fe(II)/H2O2), but the pathways leading to Fe(II) and H2O2 have remained unclear. Cellobiose dehydrogenase, purified from cultures of Coniophora puteana, will couple oxidation of cellodextrins to ...
S M Hyde, P M Wood


Extracellular carbohydrate production by isolates of Postia (=Poria) placenta
1989 - IRG/WP 1388
A monokaryotic strain of Postia (=Poria) placenta, ME20, which is unable to degrade wood, also failed to produce extracellular polysaccharide when grown in liquid culture, regardless of carbon source or concentration. Other isolates of Postia placenta, including another monokaryon and a hybrid of this monokaryon with ME20, produced large quantities of this material. The polysaccharide consisted pr...
J A Micales, A L Richter, T L Highley


Moisture Adsorption Behaviour of Rubber Wood Decayed by Brown and White rot Fungi
2005 - IRG/WP 05-40306
Moisture adsorption behaviour of rubber wood blocks decayed by each brown rot and white rot fungi was studied. The adsorption isotherms of decayed wood were compared with the isotherm of undecayed wood. The wood decayed by brown rot adsorbed less moisture as compared to sound or undecayed wood at all humidity levels. No significant difference was observed in adsorption behaviour of white rot dec...
S S Chauhan, H C Nagaveni


Pretreatment decay in poles of Pinus sylvestris
1987 - IRG/WP 1329
Storage damage by blue stain and decay in poles before impregnation has been increasingly observed during recent years and there have been reports that salt-impregnated poles today are of lower quality because of incipient decay being increasingly common. The attacks may have started at the time of cutting and then continued during the period of seasoning at the plant, which could be as long as tw...
H Lundström, M-L Edlund


Antagonism of Gliocladium virens against wood decay fungi
1995 - IRG/WP 95-10102
Antagonistic abilities of a commercial biofungicide, Gliocladium virens (GL-21, Grace) were evaluated against three white-rot fungi, Trametes versicolor, Phlebia brevispora, Irpex lacteus, and three brown-rot fungi, Postia placenta, Neolentinus lepideus, Gloeophyllum trabeum. In dual culture of Gliocladium virens and wood decay fungi, Gliocladium virens rapidly overgrow the decay fungi and killed ...
T L Highley, L Ferge


Antagonistic effects of Dacrymyces stillatus against growth of other fungi
1991 - IRG/WP 2363
The antagonistic effects in dual cultures on malt extract agar of 5 wood decay fungi and the antagonistic effects of Dacrymyces stillatus against 1 mould fungus and 1 blue stain fungus were determined. Dacrymyces stillatus was the only fungus which exhibited clear antagonistic effects. The effects of Dacrymyces stillatus against the different test fungi varied however to a great extent. Dacrymyces...
J Bjurman


Preliminary indications of the natural durability of Spruce bark board
1999 - IRG/WP 99-10312
A board material made from pressed bark, with no added adhesive, has been developed by Forintek's composites group and tested for durability by Forintek's treated-wood group and the University of Hawaii. This material was also manufactured with veneers in a one-step process. Since one role of bark on the tree is protection against pests and diseases, barkboard was expected to hav...
P I Morris, J K Grace, G E Troughton


Upgrading the fungal resistance of OSB
1999 - IRG/WP 99-40138
There is a perception that oriented strand board (OSB) is less resistant to fungl than plywood under conditions of moderate exposure to moisture. Douglas fir-faced plywood (DFP) has been suggested as a benchmark for acceptable durability under such conditions. This project was initiated to determine the minimum level of low-toxicity chemical treatment needed to upgrade the fungal resistance of OSB...
P I Morris, J E Clark, D Minchin, R Wellwood


Evaluation of hydroxamates as wood preservatives
2004 - IRG/WP 04-30330
Fungicidal activity of different hydroxamates was investigated by laboratory malt-agar block tests to evaluate their potential as wood preservatives. Results obtained indicate that benzohydroxamic acids substituted or not on the aromatic nuclei were unable to protect wood blocks against the white rot fungus Coriolus versicolor while 2-hydroxypyridine-N-oxide (2-HPNO) possesses an interesting activ...
P Gérardin, S Dumarçay, E Gelhaye, A Mbamba, V Mussavu


Preservation of basidiomycete hyphae in ancient waterlogged wood materials
1992 - IRG/WP 92-1536
Studies on waterlogged archaeological wood show that basidiomycete hyphae may persist as long as 800 years. In two pine wood samples with Phellinus pini heartrot, one from the foremast of the ship Vasa and the other from a bulwark constructed in the first part of the 12th century, numerous resin covered hyphae were observed. Hyphae with clamp connections that were associated with brown and white r...
T Nilsson, G F Daniel


Extracellular laccase production in brown-rot fungus Coniophora puteana
2001 - IRG/WP 01-10391
Coniophora puteana, one of representing brown-rot fungus, was investigated in terms of extracellular ligninolytic enzyme production. Three fungal strains of C. puteana were inoculated in liquid culture medium with sawdust of pine and oak wood. Extracellular laccase production was observed in some strains of C. puteana in the culture medium with sawdust of oak wood. The highest activity occurred at...
Kwang-Ho Lee, Seung-Gon Wi, Yoon Soo Kim


Antagonism of Scytalidium lignicola against wood decay fungi
1989 - IRG/WP 1392
Antagonistic abilities of Scytalidium lignicola against white- and brown-rot wood decay fungi were evaluated. Scytalidium lignicola did not produce inhibition zones but overgrew the decay fungi on a malt-agar medium and in most cases killed them. Pretreatment of Douglas-fir and Southern pine blocks with Scytalidium lignicola prevented decay. Blocks that were heated or treated with propylene oxide ...
T L Highley


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