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The activity of a wood-decaying fungus during drying and rewetting cycles measured by isothermal calorimetry
2013 - IRG/WP 13-20526
Wood decaying fungi are an essential part of any ecosystems as they are the main decomposers of cellulose and lignin-containing materials. But as wood is a common building material the risk for decay fungi growth and subsequent degradation of our construction material is a concern. There are important physiological aspects of the growth and activity of decay fungi that are unknown today, and witho...
S Johansson, L Wadsö, A Pilgård, G Alfredsen


Effect of biodiesel on Performance of Isothiazolone
2013 - IRG/WP 13-30625
Biodiesel has a number of attractive properties, including the ability to readily solubilize a number of biocides and a tendency to reduce odors associated with other solvents such as petroleum-based diesel. Biodiesels, however, have also been implicated in reduced performance of some, but not all preservatives. It is important to assess the effects of biodiesel on each biocide. Soil block tests ...
Cheng Hua-Kang, L Jin, C Freitag, J J Morrell


Postia placenta cellulase gene expression in modified wood during incipient decay
2013 - IRG/WP 13-40626
In optimization of modified wood, it is important to understand the mode of action of the wood modification and how the fungi response to it. The aim of this study was to investigate the expression of cellulases during the first two weeks of Postia placenta exposure in acetylated, DMDHEU-treated and thermally modified as well as in untreated wood. Using real-time PCR, the gene expression patterns ...
R Ringman, A Pilgård, K Richter


Influence of Heat Treatment Medium on Fungal Resistance of Beech Wood
2013 - IRG/WP 13-40643
Hydrothermal treatment of beech wood (Fagus orientalis) performed in acidic, neutral and alkaline buffered mediums in 160 and 180°C. White rot fungi (Coriolus versicolor) and brown rot fungi (Coniophora puteana) were used to determine the influence of heat treatment mediums on the behaviour of fungal resistance. White rot of wood digest lignin rather than cellulose; while brown rot digests the ce...
A Talaei, A Karimi, M-F Thévenon


Using hyperspectral imaging to detect and quantify mould and blue stain on coated and uncoated wood
2014 - IRG/WP 14-10822
Hyperspectral imaging has been applied on the surface of samples of coated and uncoated Norway spruce (Picea abies) as a new technique to detect and quantify growth of mould and blue stain fungi. Principal Component Analysis of the hyperspectral images resulted in a clear visualization of the fungi and the amount of mould coverage could be estimated. This indicates that NIR hyperspectral imaging c...
L Ross Gobakken, I Burud, A Flø, K Kvaal, T K Thiis


The effects of copper proximity on oxalate production in Fibroporia radiculosa
2014 - IRG/WP 14-10823
Copper remains a key component used in wood preservatives available today. However, the observed tolerance of several critical wood rotting organisms continues to be problematic. Tolerance to copper has been linked to the production and accumulation of oxalate, which precipitates copper into insoluble copper-oxalate crystals, thus inactivating copper ions. The purpose of this study was to assess d...
K M Jenkins, C A Clausen, F Green III


Protein extraction from wood decay fungus Postia placenta
2014 - IRG/WP 14-10827
Wood decay fungi (often distinguished as white rot and brown rot) belonging to the basidiomycetes, are common inhabitants of forest litter, where they play an important role in carbon cycling. Brown rot fungi are perhaps the most important organism involved in the degradation of wood products, and of considerable economic importance. Brown rotted wood loses strength very early because of the rapid...
A Pilgård, P Arnold, K Richter


The Chelator Mediated Fenton System in the Brown Rot Fungi: Details of the Mechanism, and Reasons Why it has Been Ineffective as a Biomimetic Treatment in some Biomass Applications – a Review
2014 - IRG/WP 14-10828
The chelator-mediated Fenton (CMF) reaction requires the action of two types of chelating compounds. The first chelator, oxalate, solubilizes and then sequesters iron, and the second chelator reduces iron. Iron reduction must be controlled near the fungal hyphae to prevent damaging Fenton chemistry from occurring in that location. Similarly, iron reduction must be promoted within the wood/plant ce...
B S Goodell, M Nakamura, J Jellison


A comparison of the resistance of pyrethroid-treated wood to damage by Coptotermes acinaciformis in Australia and C. formosanus in China and the USA
2014 - IRG/WP 14-10830
Field trials, using a single aboveground method of exposure, were used to assess a range of retentions of two pyrethroids (bifenthrin and permethrin) in Pinus radiata D. Don sapwood against two species of Coptotermes in three countries to provide directly comparable results. Coptotermes acinaciformis (Froggatt) in Australia, C. formosanus Shiraki in China and C. formosanus in the USA consumed simi...
J Creffield, M Lenz, D Scown, T Evans, Junhong Zhong, B Kard, J Hague, K Brown, E Freytag, J Curole, R Smith, T Shupe


Effectiveness of Copper Indicators in Treated Wood Exposed to Copper Tolerant Fungi
2014 - IRG/WP 14-20554
Wood treated with a copper based wood preservative will typically turn a green color. While the depth of copper penetration can be readily discerned from the green color of the copper it is standard practice in research and commercial treating plants to make use of a color reagent such as Chrome Azurol S, Rubeanic acid or PAN indicator to reveal the penetration more clearly. When copper treat...
L Jin, K Brown, A Zahora, K Archer


Reducing Extractives Stain in Western Red Cedar Sidewall Shingles
2014 - IRG/WP 14-30654
One of the aesthetic challenges that western red cedar shingles face is extractives redistribution stain, particularly in unpainted sidewall applications. Dip and pressure treatments with a mixture of quaternary ammonium compounds and alkyl amine oxides were investigated for their ability to prevent this stain. After nine months of exposure in Vancouver, extractives stain was present on nearly all...
R Stirling


Decay resistance of wood-plastic composites reinforced with extracted or delignified wood flour
2014 - IRG/WP 14-40655
The moisture and decay resistance of wood-plastic composites (WPCs) reinforced with extracted or delignified wood flour (WF) was investigated. Three different extractions were preformed: toluene/ethanol (TE), acetone/water (AW), and hot water (HW). Delignification (DL) was performed using a sodium chlorite/acetic acid solution. All WPCs specimens were made with 50% by weight HDPE and WF, first com...
R E Ibach, Yao Chen, N M Stark, M A Tshabalala, Yongming Fan, Jianmin Gao


Development of new wood treatments combining boron impregnation and thermo modification - Effect on decay and termites durability
2014 - IRG/WP 14-40666
Environmental pressures appeared in most European countries during the last decade has lead to the development of more environmentally acceptable preservation methods. In this context, wood heat treatment has been one of the most investigated alternative methods during the last years. Treated at temperatures ranging between 180 and 220°C, wood components undergo more or less important chemical mo...
S Salman, A Pétrissans, M-F Thévenon, S Dumarçay, B Pollier, P Gérardin


Possible targets of wood modification in brown rot degradation
2014 - IRG/WP 14-40676
Wood modification protects wood from fungal degradation in a non-toxic manner. However, the mechanisms behind the decay resistance in modified wood are currently unknown. The aim of this study was to discuss the i) colonisation, ii) nutrient recognition, iii) transcription, iv) depolymerisation and v) hydrolysis steps in the brown rot degradation progress and explore whether they are inhibited by ...
R Ringman, A Pilgård, G Alfredsen, B Goodell, K Richter


The role of hydrophobins in surface growth by the Blue Stain fungi Aureobasidium pullulans
2015 - IRG/WP 15-10837
Hydrophobins are small proteins found exclusively in filamentous fungi. These proteins are able to assemble spontaneously into amphiphilic monolayers at hydrophobic–hydrophilic interfaces. Hydrophobins have a diverse role, e.g. allowing the fungi to break through interfaces during aerial hyphae formation, sporulation, fruit body production and cell penetration. In this ongoing study, we are look...
J Stenbæk, L Riber, J Blæsbjerg Nielsen, C Møller Hansen, B Jensen


Interpretation of moisture monitoring data - Relevance of moisture content thresholds for fungal decay
2015 - IRG/WP 15-10839
It is widely accepted that besides temperature wood moisture content (MC) is the key factor when it comes to durability of wooden structures exposed to weathering. Many approaches to predict the service life of wooden structures have therefore been based on using wood MC as one major input-variable to develop dose-response relationships between material climate and decay response. However, to impr...
L Meyer, C Brischke


The whitening cause of Korean wooden heritage by flame retardant treatment
2015 - IRG/WP 15-20560
Korean wooden heritage used to treat by flame retardants in order to protect fire. There are 2 types flame retardant using for wooden heritage. These flame retardants treatment are basic measure that apply to wooden heritage along with other fire protection. The flame retardants cause white stains by reacting with Korean traditional wood painting (Dancheong) and increase wood humidity. The Korean ...
Jin Qyu Kim, Gyu-Seong Han, Yong Jae Chung, Hwa Soo Lee, Dong Won Son


Performance of kiln-dried and air-dried anti-sapstain treated pallet timber under use scenarios against wood discolouring fungi
2015 - IRG/WP 30669
During their lifespan there is a high probability that wooden pallets and packaging material will be exposed to rainfall or rewetting conditions, increasing the risk of growth of discolouring fungi. Kiln-drying of wooden pallets and packaging material without anti-sapstain treatment does not give efficient protection against wood discolouring fungi during rewetting periods. However additional temp...
A Steitz, E Stoyanova, N Pfabigan, R Gründlinger


Mould growth on spruce claddings and the effect of selected influencing factors after 4 years of outdoor testing
2015 - IRG/WP 15-30671
Norway spruce (Picea abies) is the most frequently chosen wood species as claddings in façades in Norway, and is used both coated and uncoated. The visual changes that occur during weathering can be colour changes, abrasion or wear, blistering, flaking, and even cracks in the wood or coating, but more often growth of mould and blue stain fungi is the main challenge. A field test with synchronous monitoring of relative humidity, air temperature, material temperature and wood moisture content in Norway spruce claddings has been performed in southern Norway as part of the ClimateLife project. Visual evaluation of blue stain and mould growth according to EN 927-3 was performed, and evaluation data after 46 months exposure is presented. The objectives were to study the effect of 1) environment, 2) cardinal direction and 3) colour of the coated surface on growth of blue stain and mould fungi and to study the variation in relative humidity, air temperature, wood moisture content and material temperature. After 46 months exposure, the red coating system had generally the lowest mould ratings and the uncoated claddings had the highest. Of the white coloured claddings the ones facing south had lower mould ratings than those facing north, but the opposite were found for red coloured claddings. The relative humidity was higher in front of claddings exposed northwards and in a shaded environment compared to an open environment against south. In an open environment red coloured claddings had the lowest wood moisture content and highest material temperature, and red coloured claddings against south had the highest maximum material temperature.
L Ross Gobakken, G I Vestøl


The copper-transporting ATPase pump and its potential role in copper-tolerance
2016 - IRG/WP 16-10859
Copper-tolerant brown-rot decay fungi exploit intricate mechanisms to neutralize the efficacy of copper-containing preservative formulations. The production and accumulation of oxalate is the most widely recognized theory regarding the mechanism of copper-tolerance in these fungi. The role of oxalate, however, may be only one part of a series of necessary components required for this complex mecha...
K M Ohno, C A Clausen, F Green III, G Stanosz


Production of reactive oxygen species in the presence of oxidized iron and oxalate under conditions mimicking brown-rot fungal degradation of wood
2016 - IRG/WP 16-10861
The biochemical role of oxalate in the fungal solubilization of iron is well known, but additional information is needed on the role that oxalate plays in the brown-rot decay of lignocellulose. In this study iron sequestration from iron and iron oxide-hydroxides was assessed with regard to the function of a chelator-mediated Fenton (CMF) reaction and the generation of reactive oxygen species (ROS)...
Yuan Zhu, Liangpeng Zhuang, B Goodell, Jinzhen Cao, J Mahaney


High-frequency monitoring of mass loss due to brown rot degradation of modified wood
2016 - IRG/WP 16-10862
Fungi growing in liquid culture undergoes three separate phases in which they i) adapt to the new environment, ii) grow unrestrictedly and exponentially, and iii) are inhibited to increase in number/mass due to lack of nutrients etc. Filamentous fungi have been shown to exhibit similar growth phases in a solid food substrate and have been modelled to grow in this way also in solid wood. In modifie...
R Ringman, A Pilgård, K Richter


Susceptibility of wood substrates to Aurebasidium pullulans at different temperatures
2016 - IRG/WP 16-10863
There is an increasing awareness of how the aesthetical performance of wood exposed outdoors changes over time and especially in the first few years after installation. Mould and blue stain fungi are biological agents that contribute to the weather grey colour on a wooden façade, and the blue stain fungi Aureobasidium pullulans is commonly identified as colonizer on coated and uncoated wood expos...
L Ross Gobakken, G Alfredsen


Fungicidal Effect of Tobacco Stalks (Nicotiana tabacum L.) Against Brown Rot Fungi “Coniophora puteana”
2016 - IRG/WP 16-10865
In this study it was investigated that the fungicidal effect of tobacco plant (Nicotiana tabacum L.), which is grown in many countries of the world, against brown rot fungi “Coniophora puteana”. The protective effect of this plant on Scotch pine (Pinus sylvestris L.) wood was considered by means of fungi decay test (EN 113). The wood samples have been treated by five different extracts; at 3%,...
Ü C Yıldız, S Yıldız, A Yılmaz, S Durmaz


Identification of the key factors influencing development of the blue stain fungi
2016 - IRG/WP 16-10872
Service life of wood is more and more frequently affected by aesthetic requirements than functional ones. In order to elucidate various aspects of service life, model house with façade elements made of 22 materials was built on the field test site at Department of Wood Science and Technology in Ljubljana. Colour and fungal disfigurement is among other parameters monitored on this house. In parall...
D Kržišnik, B Lesar, N Thaler, M Humar


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