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The copper-transporting ATPase pump and its potential role in copper-tolerance
2016 - IRG/WP 16-10859
Copper-tolerant brown-rot decay fungi exploit intricate mechanisms to neutralize the efficacy of copper-containing preservative formulations. The production and accumulation of oxalate is the most widely recognized theory regarding the mechanism of copper-tolerance in these fungi. The role of oxalate, however, may be only one part of a series of necessary components required for this complex mecha...
K M Ohno, C A Clausen, F Green III, G Stanosz


Untreated and copper-treated wood soaked in sodium oxalate: Effects of decay by copper-tolerant and copper-sensitive fungi
2017 - IRG/WP 17-10888
Copper is widely used as the primary component in wood protectants because it demonstrates a broad range of biocidal properties. However, a key concern with using copper in wood preservative formulations is the possibility for brown-rot basidiomycetes to resist the toxic effect. Many brown-rot basidiomycetes have evolved mechanisms, like the production and accumulation of oxalate, which helps thes...
K M Ohno, G T Kirker, A B Bishell, C A Clausen


Effects of exposure time on detection of the behavior of white-rot fungi on Oriental beech wood properties
2017 - IRG/WP 17-10902
The comparison of fungal degradation of Orientalis beechwood by two white-rot fungi was evaluated in this study. Biological, mechanical, and chemical assessments of beech wood were carried out at 30 day interval for 120 days. The results demonstrated that both fungi have greater effects on beech wood during each exposure time. However, Trametes versicolor degraded beech wood more effectively than ...
E Bari, D D Nicholas, K M Ohno


Cellulose nanomaterials in growth media for wood decay fungi
2024 - IRG/WP 24-11035
Cellulose nanofibrils (CNFs) were investigated as a partial substitution for agar in growth media for wood decay fungi. Radial growth measurements of eight basidiomycete fungi were taken on solid growth media with and without CNFs. Additionally, fungal strain virulence was evaluated using the European CSN Standard EN 113-2 wood decay durability test. The inclusion of CNFs did not significantly aff...
K M Ohno, R A Arango, R Sabo, C M Clemons, G T Kirker, A B Bishell


Cellulose nanomaterials in growth media for wood decay fungi
2024 - IRG/WP 24-11035
Cellulose nanofibrils (CNFs) were investigated as a partial substitution for agar in growth media for wood decay fungi. Radial growth measurements of eight basidiomycete fungi were taken on solid growth media with and without CNFs. Additionally, fungal strain virulence was evaluated using the European CSN Standard EN 113-2 wood decay durability test. The inclusion of CNFs did not significantly aff...
K M Ohno, R A Arango, R Sabo, C M Clemons, G T Kirker, A B Bishell


X-ray fluorescence microscopy of copper ion distribution in decay of copper-treated wood by Fibroporia radiculosa
2024 - IRG/WP 24-11047
Copper is an essential metal ion for eukaryotic life and must strictly be regulated as excess levels can cause oxidative damage, enzyme inhibition and denaturation, interruptions in nutrient transport and even cellular death. Copper-based wood treatments have efficacy against a wide range of biological organisms including decay fungi and are used in the majority of preservative formulations for ou...
K M Ohno, J Jakes, A B Bishell, L Li, Q Jin, E Maxey


Adaptability of Reticulitermes flavipes (Kollar) in a northern climate
2024 - IRG/WP 24-11048
Populations of the eastern subterranean termite, Reticulitermes flavipes (Kollar), are widely distributed across North America and have been introduced into various parts of Europe. Although colonies of this species are most abundant in the warmer, southern regions, populations have been found to persist in more northern climates where they must contend with frozen soil at least part of the year. ...
R A Arango, K M Ohno, A B Bishell, G T Kirker


Evaluation of mass timber materials for military and commercial trailer decking
2024 - IRG/WP 24-41009
In this study, CLT trailer decking was manufactured to current military trailer decking specifications, verified using PRG320 performance criteria, and treated with either waterborne or oil borne wood preservatives. Preliminary results show adequate uptake of preservative solution and subsequent testing shows minimal effect due to treatment. Field durability tests are also being conducted of treat...
G Kirker, A Senalik, X Wang, K Ohno, R Arango, M Mankowski, X Xie, J Eskola, N Kamprath, S Karwaczynski, M Weaver