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Predicting the Rate of Decay, and the Potential for Misinterpretation of Proper Scientific Method
2013 - IRG/WP 13-20525
Predicting the rate of fungal attack in wood-based materials and its effect on properties remains the holy grail of wood products pathologists; however, achieving this goal is difficult because of the many variables associated with the process. This paper reviews some of the more important aspects of the decay process in relation to test methodology and then discusses how data are sometimes used b...
B S Goodell, J J Morrell


The activity of a wood-decaying fungus during drying and rewetting cycles measured by isothermal calorimetry
2013 - IRG/WP 13-20526
Wood decaying fungi are an essential part of any ecosystems as they are the main decomposers of cellulose and lignin-containing materials. But as wood is a common building material the risk for decay fungi growth and subsequent degradation of our construction material is a concern. There are important physiological aspects of the growth and activity of decay fungi that are unknown today, and witho...
S Johansson, L Wadsö, A Pilgård, G Alfredsen


Effect of biodiesel on Performance of Isothiazolone
2013 - IRG/WP 13-30625
Biodiesel has a number of attractive properties, including the ability to readily solubilize a number of biocides and a tendency to reduce odors associated with other solvents such as petroleum-based diesel. Biodiesels, however, have also been implicated in reduced performance of some, but not all preservatives. It is important to assess the effects of biodiesel on each biocide. Soil block tests ...
Cheng Hua-Kang, L Jin, C Freitag, J J Morrell


Postia placenta cellulase gene expression in modified wood during incipient decay
2013 - IRG/WP 13-40626
In optimization of modified wood, it is important to understand the mode of action of the wood modification and how the fungi response to it. The aim of this study was to investigate the expression of cellulases during the first two weeks of Postia placenta exposure in acetylated, DMDHEU-treated and thermally modified as well as in untreated wood. Using real-time PCR, the gene expression patterns ...
R Ringman, A Pilgård, K Richter


Influence of Heat Treatment Medium on Fungal Resistance of Beech Wood
2013 - IRG/WP 13-40643
Hydrothermal treatment of beech wood (Fagus orientalis) performed in acidic, neutral and alkaline buffered mediums in 160 and 180°C. White rot fungi (Coriolus versicolor) and brown rot fungi (Coniophora puteana) were used to determine the influence of heat treatment mediums on the behaviour of fungal resistance. White rot of wood digest lignin rather than cellulose; while brown rot digests the ce...
A Talaei, A Karimi, M-F Thévenon


The effects of copper proximity on oxalate production in Fibroporia radiculosa
2014 - IRG/WP 14-10823
Copper remains a key component used in wood preservatives available today. However, the observed tolerance of several critical wood rotting organisms continues to be problematic. Tolerance to copper has been linked to the production and accumulation of oxalate, which precipitates copper into insoluble copper-oxalate crystals, thus inactivating copper ions. The purpose of this study was to assess d...
K M Jenkins, C A Clausen, F Green III


Protein extraction from wood decay fungus Postia placenta
2014 - IRG/WP 14-10827
Wood decay fungi (often distinguished as white rot and brown rot) belonging to the basidiomycetes, are common inhabitants of forest litter, where they play an important role in carbon cycling. Brown rot fungi are perhaps the most important organism involved in the degradation of wood products, and of considerable economic importance. Brown rotted wood loses strength very early because of the rapid...
A Pilgård, P Arnold, K Richter


The Chelator Mediated Fenton System in the Brown Rot Fungi: Details of the Mechanism, and Reasons Why it has Been Ineffective as a Biomimetic Treatment in some Biomass Applications – a Review
2014 - IRG/WP 14-10828
The chelator-mediated Fenton (CMF) reaction requires the action of two types of chelating compounds. The first chelator, oxalate, solubilizes and then sequesters iron, and the second chelator reduces iron. Iron reduction must be controlled near the fungal hyphae to prevent damaging Fenton chemistry from occurring in that location. Similarly, iron reduction must be promoted within the wood/plant ce...
B S Goodell, M Nakamura, J Jellison


A comparison of the resistance of pyrethroid-treated wood to damage by Coptotermes acinaciformis in Australia and C. formosanus in China and the USA
2014 - IRG/WP 14-10830
Field trials, using a single aboveground method of exposure, were used to assess a range of retentions of two pyrethroids (bifenthrin and permethrin) in Pinus radiata D. Don sapwood against two species of Coptotermes in three countries to provide directly comparable results. Coptotermes acinaciformis (Froggatt) in Australia, C. formosanus Shiraki in China and C. formosanus in the USA consumed simi...
J Creffield, M Lenz, D Scown, T Evans, Junhong Zhong, B Kard, J Hague, K Brown, E Freytag, J Curole, R Smith, T Shupe


Effectiveness of Copper Indicators in Treated Wood Exposed to Copper Tolerant Fungi
2014 - IRG/WP 14-20554
Wood treated with a copper based wood preservative will typically turn a green color. While the depth of copper penetration can be readily discerned from the green color of the copper it is standard practice in research and commercial treating plants to make use of a color reagent such as Chrome Azurol S, Rubeanic acid or PAN indicator to reveal the penetration more clearly. When copper treat...
L Jin, K Brown, A Zahora, K Archer


Decay resistance of wood-plastic composites reinforced with extracted or delignified wood flour
2014 - IRG/WP 14-40655
The moisture and decay resistance of wood-plastic composites (WPCs) reinforced with extracted or delignified wood flour (WF) was investigated. Three different extractions were preformed: toluene/ethanol (TE), acetone/water (AW), and hot water (HW). Delignification (DL) was performed using a sodium chlorite/acetic acid solution. All WPCs specimens were made with 50% by weight HDPE and WF, first com...
R E Ibach, Yao Chen, N M Stark, M A Tshabalala, Yongming Fan, Jianmin Gao


Development of new wood treatments combining boron impregnation and thermo modification - Effect on decay and termites durability
2014 - IRG/WP 14-40666
Environmental pressures appeared in most European countries during the last decade has lead to the development of more environmentally acceptable preservation methods. In this context, wood heat treatment has been one of the most investigated alternative methods during the last years. Treated at temperatures ranging between 180 and 220°C, wood components undergo more or less important chemical mo...
S Salman, A Pétrissans, M-F Thévenon, S Dumarçay, B Pollier, P Gérardin


Possible targets of wood modification in brown rot degradation
2014 - IRG/WP 14-40676
Wood modification protects wood from fungal degradation in a non-toxic manner. However, the mechanisms behind the decay resistance in modified wood are currently unknown. The aim of this study was to discuss the i) colonisation, ii) nutrient recognition, iii) transcription, iv) depolymerisation and v) hydrolysis steps in the brown rot degradation progress and explore whether they are inhibited by ...
R Ringman, A Pilgård, G Alfredsen, B Goodell, K Richter


Interpretation of moisture monitoring data - Relevance of moisture content thresholds for fungal decay
2015 - IRG/WP 15-10839
It is widely accepted that besides temperature wood moisture content (MC) is the key factor when it comes to durability of wooden structures exposed to weathering. Many approaches to predict the service life of wooden structures have therefore been based on using wood MC as one major input-variable to develop dose-response relationships between material climate and decay response. However, to impr...
L Meyer, C Brischke


The copper-transporting ATPase pump and its potential role in copper-tolerance
2016 - IRG/WP 16-10859
Copper-tolerant brown-rot decay fungi exploit intricate mechanisms to neutralize the efficacy of copper-containing preservative formulations. The production and accumulation of oxalate is the most widely recognized theory regarding the mechanism of copper-tolerance in these fungi. The role of oxalate, however, may be only one part of a series of necessary components required for this complex mecha...
K M Ohno, C A Clausen, F Green III, G Stanosz


Production of reactive oxygen species in the presence of oxidized iron and oxalate under conditions mimicking brown-rot fungal degradation of wood
2016 - IRG/WP 16-10861
The biochemical role of oxalate in the fungal solubilization of iron is well known, but additional information is needed on the role that oxalate plays in the brown-rot decay of lignocellulose. In this study iron sequestration from iron and iron oxide-hydroxides was assessed with regard to the function of a chelator-mediated Fenton (CMF) reaction and the generation of reactive oxygen species (ROS)...
Yuan Zhu, Liangpeng Zhuang, B Goodell, Jinzhen Cao, J Mahaney


High-frequency monitoring of mass loss due to brown rot degradation of modified wood
2016 - IRG/WP 16-10862
Fungi growing in liquid culture undergoes three separate phases in which they i) adapt to the new environment, ii) grow unrestrictedly and exponentially, and iii) are inhibited to increase in number/mass due to lack of nutrients etc. Filamentous fungi have been shown to exhibit similar growth phases in a solid food substrate and have been modelled to grow in this way also in solid wood. In modifie...
R Ringman, A Pilgård, K Richter


Fungicidal Effect of Tobacco Stalks (Nicotiana tabacum L.) Against Brown Rot Fungi “Coniophora puteana”
2016 - IRG/WP 16-10865
In this study it was investigated that the fungicidal effect of tobacco plant (Nicotiana tabacum L.), which is grown in many countries of the world, against brown rot fungi “Coniophora puteana”. The protective effect of this plant on Scotch pine (Pinus sylvestris L.) wood was considered by means of fungi decay test (EN 113). The wood samples have been treated by five different extracts; at 3%,...
Ü C Yıldız, S Yıldız, A Yılmaz, S Durmaz


Resistance of heat treated Ash wood under steam pressure: rot fungi, soil micro-organisms and termites
2016 - IRG/WP 16-40724
Thermal modification processes have been developed to increase the biological durability and dimensional stability of wood. The aim of this paper was to study the influence of ThermoWood® treatment intensity on wood decay resistance improvement against soil-inhabiting micro-organisms, brown/white rots and termite’s exposures. All of the tests were carried out in the laboratory with two differen...
K Candelier, S Hannouz, M-F Thévenon, D Guibal, P Gérardin, M Pétrissans, R Collet


Superhydrophobic treatment of Norway spruce wood for improvement of its resistance against brown rot and moulds
2016 - IRG/WP 16-40734
Water repellence of wood can be increased and its some other properties influenced by introduction of hydrophobic chemicals into and on cell walls without affecting the wood’s bulk density due to lumen filling. Herein, a simple dipping method to insert octadecyltrichlorosilane (OTS) into cell walls and to deposit the self-assembled hydrophobic monolayers (SAMs) of OTS is reported. It was found o...
A Kumar, P Ryparová, P Hajek, B Kričej, M Pavlič, A S Škapin, M Šernek, J Tywoniak, J Žigon, M Petrič


Role of cell wall specific moisture content on the brown-rot fungal attack on wood
2016 - IRG/WP 16-40736
Wood is a hydroscopic resource because the cell wall polymers contain hydroxyl and other oxygen-containing groups that attract moisture through hydrogen bonding. Moisture content varies with changing moisture content in its environment. The moisture content in wood is responsible for many of the performance properties we observe. The strength properties of wood are dependent on the moisture con...
R M Rowell


Untreated and copper-treated wood soaked in sodium oxalate: Effects of decay by copper-tolerant and copper-sensitive fungi
2017 - IRG/WP 17-10888
Copper is widely used as the primary component in wood protectants because it demonstrates a broad range of biocidal properties. However, a key concern with using copper in wood preservative formulations is the possibility for brown-rot basidiomycetes to resist the toxic effect. Many brown-rot basidiomycetes have evolved mechanisms, like the production and accumulation of oxalate, which helps thes...
K M Ohno, G T Kirker, A B Bishell, C A Clausen


Enhancing Our Understanding of Brown Rot Mechanisms through Catalytic Pretreatment and Cellulase Cocktail
2018 - IRG/WP 18-10909
A catalytic mechanism, described as the “chelator-mediated Fenton” (CMF) mechanism, is proposed to mimic the non-enzymatic action of brown rot fungi. A CMF treatment was used together with an enzymatic cocktail to study how wood was deconstructed and solubilized. This was done in-part to determine if the treatment mimicked the action of brown rot fungi, but also to explore improved treatment p...
S Tabor, L Orjuela, D Contreras, G Alfredsen, J Jellison, S Renneckar, B Goodell


Mechanism of nano metal fluorides against fungi
2018 - IRG/WP 18-30730
Metal fluoride nanoparticles are promising because of their low water solubility, which reduces the need for fixatives required in widely used active ingredient in wood preservatives such as copper and boron. Wood specimens treated with fluoride sols of MgF2 and CaF2 have been tested against brown-rot fungi (Coniophora puteana and Rhodonia placenta) in accordance with EN 113 and against termites (...
S M Usmani, K Klutzny, I Stephan, T Hübert, E Kemnitz


FTIR-ATR monitoring of chemical changes of thermo-chemically modified beech wood degraded by brown-rot fungus
2018 - IRG/WP 18-40823
Wood chemical modification with lactic acid oligomers (OLA) has been reported to confer promising properties for wood outdoors applications. To better understand the lactic acid interaction with wood, chemical changes following biological degradation have been characterized with a battery of tests. Fourier transform infrared (FTIR) spectroscopy has been proved to be a valuable tool for studying fu...
C Grosse, M Noël, M-F Thevenon, P Gérardin


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