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The nature of osmiophilic particles and their distribution during different stages of brown and white rot decay
1983 - IRG/WP 1213
The distribution of osmiophilic particles during the course of brown and white rot decay was investigated by applying transmission electron microscopic (TEM) methods. It was found that it correlates with the brown and white rot pattern.The osmiophilic particles are produced by the fungus and are supposed to be wood rotting enzymes....
K Messner, H Stachelberger


Targeted inhibition of wood decay fungi: degradation of cotton cellulose
1999 - IRG/WP 99-10321
Brown-rot decay is responsible for 80% of the damage and replacement of wood in service. For nearly thirty years, researchers have postulated a one-electron oxidase system combining Fe+2 oxalic acid, and H2O2 in the production of hydroxy radicals which ultimately oxidize cellulose in situ. Recently, researchers have been investigating antioxicants, in combination with biocides (Schultz, et al.,199...
F Green III, T A Kuster


Which substrate to use in a laboratory test with soft rot micro-fungi: vermiculite or unsterile soil?
1991 - IRG/WP 2372
A draft European Standard is being proposed by CEN TC 38 in which a vermiculite-burial and a soil-burial test to be carried out in succession are required. Various experiments with both procedures were set up with CCA, CC and propiconazole-formulations. The results show that vermiculite and unsterile soil yield comparable threshold values against soft rot. This poses the question of whether a two-...
A R Valcke


Effect of moisture and cellulosic substrate on decay of hardwoods by soft-rot fungi
1982 - IRG/WP 2173
The development by Forintek Canada Corp. of standard test procedures for laboratory preservative evaluations using soft-rot fungi was requested by the American Wood Preservers' Association. In Europe, through cooperative studies in the International Research Group on Wood Preservation, several attempts have been made to produce a standard procedure, but variability in results obtained bet...
A Byrne, R S Smith


Soft-rot in Tabebuia sp. wood used in water cooling tower: identification and degradation capacity of the fungi
1998 - IRG/WP 98-10253
Tabebuia sp. (ipe), a native Brazilian wood, is considered of high natural resistance to decaying fungi, and has been used in harsh environments, as cooling towers. Fifty-one fungi, belonging to mitosporic fungi group (Fungi Imperfecti), were isolated from deteriorated Tabebuia sp. wood samples, collected from the mist eliminator and packing of a cooling tower in operation for about 23 years. The ...
S Brazolin, M Tomazello, I H Schoenlein-Crusius


Soft rot fungi found in copper/chrome/arsenic treated hardwood power transmission poles in Queensland
1978 - IRG/WP 185
In Queensland the effective service life of copper/chrome/arsenic (CCA) treated hardwood telegraph and power transmission poles is being dramatically shortened due to attack by soft rot decay fungi. The Australian Wood Preservation and Pole Industries are funding research into this serious soft rot problem involving C.S.I.R.O. and the University of Queensland. The C.S.I.R.O. is actively engaged wi...
L E Leightley


The evaluation of a survey of dry rot damages in Japan
1983 - IRG/WP 1179
The damage of wooden houses from Serpula lacrymans has increased in Japan, particularly in the northern area, Hokkaido Prefecture (See Figure 1). The weather conditions in Asahikawa have been recorded. The data obtained from the survey carried out over the five years from 1975 to 1980 were previously reported to the 5th International Biodeterioration Symposium which was held in Aberdeen, Scotland ...
S Doi


A possible role of unique TCA cycles in wood-rotting basidiomycetes
2003 - IRG/WP 03-10461
The copper tolerant brown-rot fungus, Fomitopsis palustris, acquires metabolic energy by use of the constitutively-occurring Kornberg’s glyoxylate cycle coordinating with oxalate biosynthesis and glucose oxidation (Erman Munir et al. Proc. Natl. Acad. Sci. USA, (2001) 98, 11126-11130). Furthermore, this fungus does not have the normal TCA cycle, lacking 2-oxoglutarate dehydrogenase which is a ke...
E Munir, T Hattori, M Shimada


The use of selective media for studying the colonization of wood in ground contact by microorganisms
1978 - IRG/WP 186
In October1976 a field trial was set up in order to monitor the progressive colonisation of wood in ground contact using orientated small stakes of Scots pine (Pinus sylvestris) and birch (Betula sp.) (30 x 45 x 250 mm³). Half the number of each species were treated with a 1% solution of CCA by a vacuum impregnation process. All the stakes were coated on three sides with an epoxy-resin sealant to...
C P Clubbe


Natural Durability studies and Changes in wood chemistry of some Ghanaian hardwoods during decay by white- and brown-rot fungi
2004 - IRG/WP 04-10542
The natural decay resistance of Esa (Celtis mildbraedii), Wawabima (Sterculia rhinopetela) and Dahoma (Piptadeniastrum africanum) obtained from Ghana was determined according to BS EN 113 (1997). Esa was found not durable to the white-rot, but very durable to the brown-rot; Wawabima was very durable to both white- and brown-rots; and Dahoma was durable to the white-rot, and very durable to the bro...
Zeen Huang, K Maher, S A Amartey


Detection of dry rot by air analysis
1992 - IRG/WP 92-2399
Detection of dry rot (Serpula lacrymans) at an early stage appears to be very difficult in practice. Also control inspections in buildings, after remedial treatment of dry rot, have a limited accuracy. The use of trained dogs in Denmark initiated the idea for this research on the possible use of air analysis as a detection method. The Centre for Timber Research-TNO (TNO-CHT) and the Institute for ...
P Esser, A C Tas


The colonisation and succession of fungi in wood
1980 - IRG/WP 1107
A study of the microbial ecology of small stakes of Pinus sylvestris and Betula pendula, both untreated, and treated with a 1% solution of CCA is in progress. Results are presented for the early stages, up to 18 months.Two new techniques were developed linked by the philosophy of objective assessment. Isolations were made from the wood in a pre-determined pattern and transferred to four selective ...
C P Clubbe


Colonization of treated and untreated ponderosa pine exposed in Hilo, Hawaii
1995 - IRG/WP 95-20068
The rate of decay in above ground exposures is largely controlled by rainfall and temperature, factors which can be used to construct a climate index of decay hazard in above ground exposures. Developers of new biocide formulations have utilized this knowledge by establishing test sites in sub-tropical regions such as the Gulf Coast of the United States. More recently, field sites have been locate...
C M Freitag, J J Morrell, K J Archer


An alternative method for the determination of lignocellulolytic enzymatic activities of white rot fungi
1998 - IRG/WP 98-10261
Some lignocellulolytic activities of wood decay fungi can be evaluated in an indirect way through the study of the decolorization that white rot fungi produces in culture media in the presence of dyes. In this assay, ten dyes were selected, which were added in concentrations of 0.00 1% - 0.003% (P/V) to four-day cultures of Trametes versicolor containing 1% of beech sawdust in a basal salt medium...
D Muñoz-Mingarro, F Llinares, F Rubio, M Yuste, C Rodríguez-Borrajo, J Jiménez, M T De Troya


The production of extracellular hydrogen peroxide by some brown-rot fungi
1990 - IRG/WP 1446
The role of hydrogen peroxide (H2O2) has been discussed in the degradation of wood by wood-rotting fungi. The production of extracellular hydrogen peroxide was studied by detecting the oxidation of the chromogen 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) by H2O2 and horse radish peroxidase (HPR). ABTS and HPR were added to a solid wood based culture media. In this study ...
A-C Ritschkoff, L Paajanen, L Viikari


Fungal detoxification of organotin biocides
1985 - IRG/WP 1258
The ability of a range of wood decaying fungi to inactivate bis(tri-n-butyltin) oxide (TnBTO) in the extracellular growth medium, in stationary liquid culture was determined. A distinction between the ability to tolerate the fungicide and to inactivate it was made: the white-rot organism Coriolus versicolor being the most efficient inactivator. In an attempt to determine the extracellular agents r...
P S Belford, D J Dickinson


Evaluating the performance of wood preservatives against fungi
1974 - IRG/WP 247
A compilation is made of results published by various workers for agar/block laboratory tests of the effectiveness of wood preservatives against 11 species of wood destroying Basidiomycetes and one species of soft rot fungus. Preservatives include creosote, boron, metal/arsenic mixtures, metallic naphthenates, chlorinated naphthalene, chlorinated phenols and tri-n-butyl tin oxide. The results are ...
R Cockcroft


Cation analysis of wood degraded by white and brown rot fungi
1992 - IRG/WP 92-1552
Changes in the concentrations and composition of cations within wood colonized by the brown-rot fungi Postia placenta and Gloeophyllum trabeum and the white-rot fungi Trametes versicolor and Phanaerochaete chrysosporium were analyzed using plasma emission and atomic absorption spectrophotometry. In degraded poplar wood significant decreases in pH and electrical resistance readings were observed as...
J Jellison, K C Smith, W T Shortle


Extracellular osmiophilic particles in connection with brown rot and white rot
1982 - IRG/WP 1157
The way of attack on Pine woodblocks of two brown-rot fungi (Fomitopsis pinicola, Coniophora puteana) differing in their cellulase activities, and one white-rot fungus (Trametes hirsuta) has been investigated by transmission electron microscopy. Even at early stages of decay, osmiophilic particles were found with all the fungi investigated. In the brown-rotted wood, the osmiophilic particles occur...
K Messner, H Stachelberger


Production, function and neutralization of oxalic acid produced by the dry rot fungus and other brown rot fungi
1987 - IRG/WP 1330
The formation of oxalic acid by the wood-destroying fungi causing brown rot, is found to be the key which by hydrolysing the hemicellulose brings the cellulose in the tracheid wall in contact with the cellulase enzymes and yeld watersoluble sugars leaving only a lignin skeleton. To control the pH in the substrate the excess oxalic acid is precipitated to water insoluble calcium oxalate by the dry ...
J Bech-Andersen


Hydrolysis of bordered pits during colonization of conifers by brown-rot fungi
1995 - IRG/WP 95-10103
Brown-rot decay results in rapid reduction in degree of polymerization (DP) of holocellulose with concomitant strength loss (MOR) without removing lignin. Development of new methods of wood protection will require focusing on early events in the sequence of depolymerization. Bordered pit membranes (sapwood) represent a readily available source of non-lignified carbohydrate, ie. pectin and cellulos...
F Green III, J L Tschernitz, T A Kuster, T L Highley


The involvement of extracellular substances for the generation of hydroxyl radical during wood degradation by white-rot fungi
1997 - IRG/WP 97-10218
The activities of one-electron oxidation, hydroxyl radical generation, and phenol oxidase during the incubation of cultures of four white-rot fungi containing either glucose or wood were periodically measured. Further, their degradation activities against wood were examined during the course of cultivation. The generation of hydroxyl radical was correlated to the activity of wood degradation and i...
H Tanaka, S Itakura, A Enoki


Antagonistic properties of Gliocladium virens against wood attacking fungi
1996 - IRG/WP 96-10162
Gliocladium virens has shown good antagonism against decay fungi in agar medium and in wood blocks. Gliotoxin produced by Gliocladium virens is associated with biocontrol of some plant diseases, but its importance to biocontrol of wood-attacking fungi is unknown. We investigated the ability of gliotoxin-producing (GLT+) isolates of Gliocladium virens and gliotoxin-deficient (GLT-) mutants of Glioc...
T L Highley, H S Ananthapadmanabha, C R Howell


Investigations on testing with soft rot fungi by the vermiculite-burial method
1972 - IRG/WP 215
With the object of developing a test method to determine the effectiveness of wood preservatives against soft rot attack, tests have been carried out with pure fungal cultures using vermiculite as the substrate for untreated and preservative impregnated wood blocks. In these tests the suitability of common commercial, simple disposable beakers was proved successful as a substitute for the glass bo...
W Kerner-Gang, M Gersonde


The importance of blue stain attack for the colonization by wood-rotting fungi of wood not in contact with the ground
1988 - IRG/WP 1349
When used in constructions not in contact with the ground, wood has been shown often to proceed from blue stain to moulds. The appearance of wood rotting fungi is normally delayed. Solid wood artificially inoculated with the blue stain fungus Pullularia pullulans was shown to permit germination of Pycnoporus cinnabarinus basidiospores. This was demonstrated by the use of an indirect and a direct b...
J Bjurman


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