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Measurements of rot fungal activity as a function of moisture content by isothermal calorimetry
2010 - IRG/WP 10-20428
Measurements of heat production rate have been made on wood samples with the brown rot fungus Postia placenta at different moisture contents. The results clearly indicate that the heat production rate (a measure of respiration rate and activity) is moisture dependent. When the moisture content is decreased, less heat is produced, and when the moisture content is increased, more heat is produced. I...
L Wadsö, A Pilgård, G Alfredsen


Detection of Wood Destroying Fungi Using DNA Microarray Technology
2010 - IRG/WP 10-20435
Wood decay fungi of the phylum Basidiomycota cause serious damage to wooden constructions and building elements. The elimination and the appraisement of fungal decay require an assured species identification. Conventional fungal diagnostics are mainly based on morphological characteristics by macro- and microscopy analysis. For some years, standardised and highly sensitive molecular methods focuss...
K Jacobs, N Rangno, W Scheiding, B Weiss, D Müller, C Hiller, W Brabetz


Analyzing hyphal growth of the bio incising fungus Physisporinus vitreus with light-, confocal laser scanning- and, Synchrotron X-ray tomographic microscopy
2010 - IRG/WP 10-20438
Norway spruce wood is an important building material for the forest and wood industry. To improve wood permeability and uptake of wood modification substances Physisporinus vitreus is employed as a bioincising agent. In this study wood specimens were incubated with P. vitreus to assess hyphal colonisation and structural alterations of Norway spruce wood. After different incubation periods, semi-th...
C Stührk, M Fuhr, M Schubert, F W M R Schwarze


Profiling fungal community in wood decay ecosystem by Denaturing High-Performance Liquid Chromatography
2010 - IRG/WP 10-20443
A DNA-based fingerprinting technique, Denaturing High Performance Liquid Chromatography (DHPLC) was developed to profile fungal communities colonizing indoor timber. Molecular fungal diversity was assessed using amplification based on the Internal Transcribed Spacer (ITS1) rDNA of 74 wood samples collected from infected buildings in France. Due to its high sensitivity, the PCR-DHPLC technique was ...
S Maurice, G Le Floch, M Le Bras-Quéré, J P Rioult, G Barbier


Onset of mould growth – the effect of climate variability and different geographic locations
2010 - IRG/WP 10-20446
This paper presents a study on how climate conditions at numerous locations in Sweden affect the risk of onset of mould on wood sheltered outdoors. The results indicate that the risk is clearly higher in the south compared to the north of Sweden. These differences are mainly explained by colder and dryer winter periods in the north which is unfavorable for development of mould. The results also sh...
M Häglund, T Isaksson, S Thelandersson


Drying Rates and Mold Growth on Various Building Materials under Different Environmental Conditions
2010 - IRG/WP 10-20454
Mold growth on building materials is a major problem for homeowners. The most suitable method to control mold growth on building materials is to utilize design features, construction tools and practices that prevent moisture accumulation, and keep the wood as dry as possible. In order to achieve this, engineers and homebuilders have to know the effects of various temperature and moisture conditi...
Dian-Qing Yang


Mould growth on wood-based materials – a comparative study
2010 - IRG/WP 10-20455
Ten different wood-based materials - preservative-treated wood, fire retardant-treated wood, modified wood, WPCs and untreated references of pine sapwood and spruce - were tested for mould growth according to SP method 2899 during 42 days at 90% RH and 22°C. Even though the results must be interpreted carefully, they indicate significant differences in mould resistance between the materials tes...
P Johansson, J Jermer


Comparison of Bifenthrin Formulations for Their Skin and Eye Irritation Potential Using the Pollen Tube Growth Test
2010 - IRG/WP 10-30521
The Pollen Tube Growth Test (PTGT) is an effective screening method for measuring the potential of a chemical formulation to affect living cells. The PTGT has been compared against other types of testing, and it shows a good correlation with, for example, the Draize rabbit eye test and the human skin patch assay. It measures the EC50, which is the effective concentration at which 50% growth is o...
S Millward, P Lobb


Effects of polyvinyl alcohol on leachability and efficacy of boron wood preservatives against fungal decay and termites attack
2010 - IRG/WP 10-30526
In this study, the use of polyvinyl alcohol (PVA) as boron fixative agent was investigated. Two levels of PVA (2.5 and 4%) were evaluated with disodium octaborate tetrahydrate (DOT) at three concentrations (1, 2 and 4% boric acid equivalent (BAE) using a double vacuum impregnation processes on Scots pine sapwood specimens. Leaching was performed according to a laboratory leaching procedure. Boron ...
A Mohareb, M F Thévenon, E Wozniak, P Gérardin


Effects of Cunninghamia Lanceolata Heartwood Extracts on the Growth of Wood Decay Fungi
2010 - IRG/WP 10-30527
China-fir (Cunninghamia lanceolata) wood is well known for its good durability. This species is native to China and has been extensive planted there. Mainly, extractives are responsible for the wood resistance to decay fungi. In this paper, the extractives of China-fir heartwood were studied for their effects on various wood decay fungi. Sequential extraction of heartwood in hexane, ethyl acet...
Shujun Li, Jing Wang, Jian Li


Laboratory evaluation of borate amine: Copper derivatives in wood for fungal decay
2010 - IRG/WP 10-30543
The aims of this study were to evaluate borate: amine: copper derivatives in wood for fungal decay protection as well as the permanence of copper and boron in wood. Wood treated with each of four derivatives of borate:amine:copper prevented fungal decay. Disodium tetraborate ecahydrate (Borax):amine:copper derivatives with retentions of 0.61 to 0.63% after water leaching prevented decay by Gloeo...
G Chen


Preventing decay in termite monitoring stations
2010 - IRG/WP 10-30546
In-ground monitoring stations and termite baits are widely used in the southern United States with the intent of detecting and eradicating subterranean termite activity near buildings. These stations comprise a wood (or other cellulose material) substrate (monitor) and perhaps a termiticidal toxicant (bait) held in a plastic cage that allows for both easy examination and access by termites. The us...
A M Taylor, Jae-Woo Kim, S Duarte, L Nunes, J D Lloyd


Fungal decay resistance and mechanical properties of plywood panels made from maritime pine (Pinus pinaster) and bonded with cornstarch-quebracho tannin-phenol formaldehyde adhesive
2010 - IRG/WP 10-40490
The aim of this work is to demonstrate the performances of cornstarch-quebracho tannin-based resins designed as adhesive in the plywood production. In this way, the cornstarch and quebracho tannin was introduced in the classic adhesive formulation in order to supply a part of phenol-formaldehyde (PF). In order to evaluate the mechanical performances of optimal cornstarch-quebracho tannin-PF, plywo...
F Charrier, A Moubarik, A Allal, A Pizzi, B Charrier


Moisture dynamics of WPC as basis for biological durability
2010 - IRG/WP 10-40520
The largest market for wood-polymer composites (WPCs) is currently decking. Although many products are commercially available, a proper standard for the assessment of the biological durability of WPC does not exist. Recommended standards for testing resistance against basidiomycetes should be completed with a method to bring the specimens in a worst case situation, obtaining a moisture level high ...
N Defoirdt, J Van Acker, J Van den Bulcke


Effects of global climate change on mould growth - Interactions of concern
2010 - IRG/WP 10-50270
An evident change in climate the last decades has been recorded, and combined effects of increased CO2, elevated temperature and altered precipitation regimes have been observed to represent a change to the fundamental drivers within ecosystems. Growth of moulds, both in nature and on man-made constructions and objects, will most likely increase due to changes in the climate. The survival, the rep...
L Ross Gobakken


Fungal biodegradation of CCA-treated wood wastes
2011 - IRG/WP 10-50276
Fungal biodegradation could be employed as a pretreatment method to alleviate problems caused by landfill disposal of CCA-treated wood wastes (e.g. a shortage of landfill space and a release of leacheate). It could be used to decrease the volume of waste and to remove metals from waste simultaneously. We have screened hundreds of decay fungi, including fungi isolated from CCA-treated wood in servi...
Gyu-Hyeok Kim, Yong-Seok Choi, Jae-Jin Kim


Basidiomycete colonization of Scots pine sapwood quantified by qPCR and TGA
2011 - IRG/WP 11-10750
Scots pine (Pinus sylvestris L.) is an important softwood species in Northern Europe and is frequently used as material for various wood protection systems. In Europe, EN 113 is the standard basidiomycete laboratory durability test method, using mass loss as evaluation criteria. In this paper quantitative real-time PCR (qPCR) and thermogravimetric analysis (TGA) was used to characterize coloniza...
G Alfredsen, S Bollmus, T K Bader, K Hofstetter


The effects of acetylation level on the growth of Postia placenta
2011 - IRG/WP 11-10751
To understand the defence mechanisms utilized by decay fungi when exposed to different wood protection systems the study of gene expression can give us some answers. When the DNA sequences are known, primers can be designed to detect transcripts of genes with gene products related to basic cellular processes and hyphal growth. The characteristic gene products induced in different fungi by differen...
A Pilgård, G Alfredsen, C G Fossdal, C J Long II


Measuring fungal cellulolytic enzyme activity in degrading wood
2011 - IRG/WP 11-10753
An enzyme assay which measures enzyme activity directly from wood will assist in the fundamental understanding of the enzyme components of the decay mechanism of rot fungi and any changes in the presence of wood protectants. At present, to measure fungal carbohydrate degrading enzyme activity in the presence of wood, two methods are used: either 1) the enzyme is measured in the growth media contai...
S M Duncan, J S Schilling


Molecular investigation of Postia placenta growing in modified wood
2011 - IRG/WP 11-10756
Brown rot is the most common and destructive type of fungal decay for wood in service. These fungi depolymerize preferentially the structural carbohydrates, cellulose and hemicellulose in the cell wall leaving oxidized lignin behind. Modified wood can provide protection against a variety of wood deteriorating organisms, including decay fungi. However, there is still little known about the mode of ...
B Schmöllerl, G Alfredsen, C G Fossdal, M Westin, A Steitz


The influence of moisture content and wood pH variation on fungal melanin formation in wood substrates
2011 - IRG/WP 11-10759
Wood decay patterns are strongly influenced by the conditions of the wood substrate, including moisture content and pH. In an antagonistic environment some fungi respond with pigment formation that helps to isolate and protect their mycelium. This is often associated with slower fungal growth and delay of wood mass loss. Dark colored melanin is the most common pigment formed by wood decay fungi, a...
D Tudor, S C Robinson, P A Cooper


Durability testing of coconut shell according to ENV 807
2011 - IRG/WP 11-10761
Coconut shell was tested in the laboratory according to the European standard ENV 807 with three different soil types: compost soil, brown rot/soft rot rich soil and white rot/soft rot rich soil. Mass losses between 14 and 16 % were achieved with all three soils, indicating that the decay type is of little importance in the degradation process. Somewhat higher mass losses, 19-22 % were obtained fo...
J Jermer, A H H Wong, K Segerholm, T Nilsson


A comparative study and evaluation of methodologies used for determining wood preservative penetration
2011 - IRG/WP 11-20475
A series of methods for determining penetration of wood preservatives into the wood structure have been developed for either quality control (QC) or research and development purposes. QC methods range from monitoring the solution uptake, applying colour indicators or sampling specific wood samples followed by wood acid digestion/atomic absorption spectroscopy (AAS) or solvent extraction/chromatogr...
R Craciun, R Moeller, J Wittenzellner, T Jakob, J Habicht


Decay factors in termite in-ground monitoring stations
2012 - IRG/WP 12-10775
Subterranean termites are serious pests of wood in service in much of the world. One of the most common techniques for monitoring and controlling termites is the use of in-ground monitoring stations which comprise a wood or other cellulosic material monitor (cellulosic matrix) and/or a termiticide bait held in a open plastic holder so facilitates examination and the termite access. Wood and other ...
S Duarte, A M Taylor, Jae-Woo Kim, J D Lloyd, M Duarte, L Nunes


Connecting fungal genomic variations to wood chemical composition: A way to understand the adaptation of wood decaying fungi
2012 - IRG/WP 12-10776
The development of new sequencing methods allows now exploring the detoxication systems of wood-decaying fungi. These systems, called xenome, are mainly composed of oxidative enzymes (phase I) and of conjugation enzymes (phase II). These enzymes belong to superfamilies, which are extended in the plant cell-wall interacting fungi. From different approaches coupling in particular comparative genomic...
E Gelhaye, Y Mathieu, E Meux, A Thuillier, M Mélanie


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