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Effect of volatiles from bacteria and yeast on the growth and pigmentation of sap-stain fungi
2000 - IRG/WP 00-10331
Sapstain fungi cause deterioration of wood due to colonisation by pigmented hyphae but without producing significant strength losses. This is due to the production of melanin in the fungal cell walls of the staining fungi. Any biological control strategy targeted against this type of deterioration would therefore be considered successful if it inhibited either fungal growth or pigment production. ...
A Bruce, R E Wheatley, S Verrall


Variation in natural durability of British grown Douglas fir ((Pseudotsuga menziesii (Mirb.) Franco). Part I. Effect of density and growth rate
2002 - IRG/WP 02-10445
Seed origin trials of Douglas fir (Pseudotsuga menziesii) in Britain have provided important information on growth differences between origins (Lines, 1987), but the variation in wood properties between origins has not been reported. The present paper describes a study of the variation in natural durability against two brown rot fungi (Coniophora puteana and Postia (Poria) placenta) and in a fung...
S Akhter, M D C Hale


Growth of selected wood decay fungi on various agar-supplemented media
2003 - IRG/WP 03-10456
The growth rates of a selection of wood decay fungi (brown and white rots) on various agar-supplemented media have been determined and compared. The agar media investigated were Malt extract agar (MEA), Potato dextrose agar (PDA), YMPG agar (yeast extract, malt extract, bacto-peptone, glucose, asparagine and thiamine), YMPG agar (without amino acids) and Beech wood powder agar (BWA). The tested wo...
S A Amartey, M Humar, F Pohleven


The effect of Trichoderma volatiles on the growth and enzyme production of Serpula lacrymans
2000 - IRG/WP 00-10332
Although various authors have reported the biological control of Serpula lacrymans by Trichoderma spp. the mode of antagonism employed by Trichoderma is not yet clear. The work presented here concentrates on the production of anti-fungal volatiles for inhibition of S. lacrymans growth. Volatile mediated interactions were examined between four S. lacrymans isolates and a range of nine known Trichod...
S N Humphris, A Bruce, R E Wheatley


The WOODCARE project: Development of detection methods for Death watch beetle larvae and fungal decay
1999 - IRG/WP 99-20172
Woodcare was a European project coordinated by English Heritage. The aim of the research was to develop more targeted and more environmentally friendly treatment methods for Death watch beetle infections in Oak constructions of historical buildings. TNO has developed two new methods for fast and reliable detection of Death watch beetle larvae and related fungal decay in Oak. The problem with Death...
P Esser, P Van Staalduinen, A C Tas


Laboratory testing of wood natural durability - In soil-bed assays
1998 - IRG/WP 98-20141
Laboratory methods for assessing wood decay resistance are being investigated in the framework of an ongoing European research project. This paper summarizes the main results obtained for soil-bed tests based on the European prestandard ENV 807-Test 2. The data suggest that this testing methodology is suitable for evaluating the natural durability of timber species in soil-contact under conditions...
R Sierra-Alvarez, I Le Bayon, J K Carey, I Stephan, J Van Acker, M Grinda, G Kleist, H Militz, R-D Peek


Aspects of the fungal degradation of quaternary ammonium compounds in liquid culture
1997 - IRG/WP 97-30160
Didecyldimethylammonium chloride (DDAC) is a quaternary ammonium compound (QAC) that has found use as an anti-sapstain preservative for the transportation of softwood lumber from Canada to overseas markets. However, its use is limited by the knowledge that certain mould fungi seem capable of degrading it. The aim of this research was to gain knowledge about the fungal degradation of DDAC. The effe...
J W Dubois, J N R Ruddick


Laboratory trial to identify potential in-forest treatments to control fungal pre-infections of radiata pine logs
2000 - IRG/WP 00-30220
Development of fungal infections of radiata pine logs by wood degrading fungi commences immediately after tree felling. In general, subsequent antisapstain treatments are unable to control fungal degrade on logs where the pre-treatment log storage time exceeds 2-5 days. However, use of an in-forest treatment of logs may be advantageous to log exporters to control fungal pre-infections during the p...
D R Eden, B Kreber, R N Wakeling, J G Van der Waals, C M Chittenden


Respiration measurement of dry-rot
1995 - IRG/WP 95-10095
Methods for measuring the production of carbondioxide from wood infected with the dry-rot fungus were evaluated. By enclosing whole wood blocks and measuring the concentration of gas in the headspace by gas chromatography, an exponential increase in the concentration of CO2 was observed for at least 2 months. This technique could therefore present a method for evaluating various treatments of dry-...
L Toft


A non-pressure method of protection based on hurdle theory to control the spectrum of internal environmental factors which affect the decay of poles in soil contact
1993 - IRG/WP 93-20005
A field trial was conducted to establish whether superficial barrier linings on poles in soil contact could function as environmental hurdles against the growth of biological agents and thus provide preventative methodology to preclude premature failure of vineyard poles under flood-irrigation. Assessment after 52 weeks exposure to the prevailing conditions and sub-tropical environment showed that...
A A W Baecker


Fungal colonisation of the keelson and associated structures of a nineteenth century wooden frigate: Concepts of community structure and development
1994 - IRG/WP 94-10072
The early stages of microbial colonization and succession of wooden surfaces exposed to the sea have been extensively studied as have the community structures of archaeological timbers subjected to submergence and then retrieval from the sea. The frigate UNICORN, a largely intact 19th century wooden ship based in Dundee, Scotland, provides the microbial ecologist with a unique opportunity to study...
N A White, J W Palfreyman, G M Smith


Evaluating the resistance of wood-based panel products to fungal attack
1995 - IRG/WP 95-20071
At present there is considerable disagreement among national research institutes within Europe and panel product manufacturers on the most appropriate method of testing and indeed the need for specific biological durability testing. This paper seeks to place before a broader international audience, the issues related to development of a European standard for evaluating the resistance to fungal dec...
R G Lea, R W Berry


Effects of cyproconazole and copper sulphate on the length of the hyphal growth unit (HGU) of the white-rot fungus Coriolus versicolor
2003 - IRG/WP 03-10473
Wood decay basidiomycetes have been shown to produce appreciable quantities of extracellular mucilaginous materials (ECMM). The relationship between ECMM and total biomass production has been investigated in the white-rot fungus Coriolus versicolor (CTB 863 A). Differences in the amount of ECMM produced by the fungus proportionally to the total biomass, were observed under a range of physiological...
D Vesentini, D J Dickinson, R J Murphy


Mechanisms of Protection by NHA Against Fungal Decay
2002 - IRG/WP 02-10429
Treating wood with the water-borne sodium salt of N'-N-naphthaloylhydroxylamine (Na-NHA) protects wood against decay and termite damage. Initial testing indicated little or no inhibition of sapstain fungi, molds, or soft-rot fungi by Na-NHA, suggesting that the mechanism by which this compound protected wood was complex and not that of a broad-spectrum biocide. Previously, we (Green et...
F Green III, W Henry, T Schultz


Screening of the efficacy of tall oils against wood decaying fungi
2004 - IRG/WP 04-30354
Tall oil is a by-product in pulping of resinous wood by the sulphate process. Tall oil contains a complex mixture of wood extractives. Some of these extractives act as natural protection against wood decaying fungi while other serve as nutrition for the fungi. This report describes a screening of the efficacy of four refined tall oils with different chemical composition on wood decaying fungi. ...
G Alfredsen, P O Flæte, A Temiz, M Eikenes, H Militz


Blue stain in service on wood surface coatings. Part 1: The nutritional requirements of Aureobasidium pullulans
1992 - IRG/WP 92-1556
The nutritional requirements of Aureobasidium pullulans was examined with regard to nutrient sources that are potentially available in fresh and weathered wood. The study was designed to determine how far wood cell wall components need to be broken down during weathering before they provide a useful nutrient source for Aureobasidium pullulans. Various carbon sources were tested, with eight differe...
P R Sharpe, D J Dickinson


Soil treatment tests with the three products of boric acid for the prevention of the hyphal growth of Serpula lacrymans
1992 - IRG/WP 92-3693
Laboratory soil treatment tests were conducted for the evaluation of fungicidal or fungistatic effect of boric acid products against Serpula lacrymans. Boric acid products tested were the following three: a thickened boric acid solution in triethanolamine, boric acid-silica gel complex granules, and a nonwoven fabric laminated with a polypropylene film and coated with boric acid granules on one si...
S Doi, A Yamada, Y Mineki, M Mori


Effect of some resin treatments on fungal degradation reactions
1999 - IRG/WP 99-10318
The effect of impregnation of wood with vegetable oil, tall oil and two water soluble on fungal degradation reactions was assessed by hydrolysis and enzyme activity measurements. The experiments were carried out by using isolated enzyme preparations and a living brown-rot fungus Poria placenta. The results indicate that the inhibitory effect of treatments was clearly directec towards the enzymatic...
A-C Ritschkoff, M Rättö, A J Nurmi, H Kokko, A O Rapp, H Militz


Growth of sapstain fungi in scots pine and the effect of timber ageing
1998 - IRG/WP 98-10269
A trial was initiated to study the growth of known isolates of sapstain fungi in scots pine (Pinus sylvestris) which had been naturally aged or sterilised by autoclaving or irradiation. Sawlogs cut from commercial thinnings were reduced to billet size and the exposed ends were painted with a bituminous sealant to reduce moisture loss. Some billets were inoculated after autoclaving or gamma-irradia...
N J Strong, J F Webber, R A Eaton


Some studies on fungal deterioration of rubber wood (Hevea brasiliensis)
1980 - IRG/WP 2140
For the sreening of anti-stain chemicals trials with selected agricultural fungicides and new chemicals were carried out. Botryodiplodia theobromae, Aspergillus sp. and Penicillium sp. were used at test organisms. For testing the durability of rubber wood (Hevea brasiliensis) suitable local rotting fungi (Basidiomycetes), based on high degrading power, which may later be employed in standard tests...
A Sujan, A G Tan, M Stevens


An attempt to grow dry rot fungus Serpula lacrymans on mortar substrate in non-sterile conditions
1989 - IRG/WP 1385
According to many experts, Serpula lacrymans is probably the most dangerous wood destroying fungus in houses. Investigations of KOCH, A.P. (2) showed that incidence of this fungus in percent was around 20% when the frequency of some other standard fungi was far below (Coriolus versicolor: 0.2% for example). The work presented here is a part of a program carried out at CTFT for assessing the resist...
L N Trong


Fungal siderophores and their rôle in wood biodegradation
1990 - IRG/WP 1442
Iron and other metals such as manganese, play an important role in the metabolic functions of fungi that cause wood deterioration. These transition metals are also found in, or associated with, the extracellular fungal enzymes shown to be directly involved in the decay process. Recently our research group was able to show that siderophores (low molecular weight biological chelators) are produced b...
J Jellison, B Goodell, F Fekete, V Chandhoke


Sapstain development on Jack pine logs in Eastern Canada
2000 - IRG/WP 00-10358
During 1998-99, a study was initiated to investigate the influence of seasons, log types and storage time on the sapstain development on jack pine logs at two sites in Eastern Canada. Jack pine trees (Pinus banksiana) were harvested into whole-tree and cut-to-length logs in spring and in autumn. Sapstain development was examined in these logs at 2 to 4 week intervals after felling. The mean stain ...
Dian-Qing Yang, R Beauregard


A rearing procedure for the house longhorn beetle Hylotrupes bajulus L
1973 - IRG/WP 228
The technique practised at the Princes Risborough Laboratory for culturing Hylotrupes bajulus is described. It is modified from methods previously suggested by German workers. A protein and vitamin enriched wood diet to accelerate larval growth is employed and larvae are subsequently given a period of cool storage to induce pupation. Adults are confined in groups with prepared surfaces for egg lay...
R W Berry


Regulation of hyphal sheath formation iron-chelator production by the brown rot fungi Gloeophyllum trabeum and Postia placenta
1994 - IRG/WP 94-10074
Hyphal sheath formation and iron-chelator production by liquid cultures of the brown rot fungi Gloeophyllum trabeum and Postia placenta were monitored over time. The effects of incubation at various temperatures, pH's and concentrations of iron, manganese and nitrogen were determined. Hyphal sheath production was observed microscopically and quantified with JAVA video analysis. The hyphal...
Y Chen, J Jellison


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