Your search resulted in 116 documents. Displaying 25 entries per page.
Attempts to isolate tunnelling bacteria through physical separation from other bacteria by the use of cellophane
1992 - IRG/WP 92-1535
During attempts to isolate tunnelling bacteria in pure culture, physical separation from other bacteria was successfully achieved by the use of cellophane. Cellophane was used in liquid cultures as a substrate for tunnelling bacteria that were present in a mixture with other bacteria. By tunnelling into cellophane, TB were physically removed from contaminating bacteria which could then be removed ...
T Nilsson, G F Daniel
Visualising Bacteria in Wood Using Confocal Laser Scanning Microscopy (CLSM)
1998 - IRG/WP 98-10272
A fluorescent phospholipid probe was used in conjunction with confocal laser scanning microscopy (CLSM), to visualise bacteria which inhabit in radiata pine wood and degrade pit membranes. CLSM has the ability to collect fluorescent images through different emission filters at the same time, so it is possible to distinguish gram-positive and gram-negative bacteria in infected wood by counterstaini...
Ying Xiao, A P Singh, R N Wakeling
Detoxification of methylene bisthiocyanate by bacteria isolated from freshly felled, anti-sapstain treated timber
1993 - IRG/WP 93-10017
The microbial defacement of freshly-felled timber treated with organic biocides continues to be a major problem in stored lumber. As part of a study to investigate the depletion in chemical activity of methylene-bis-thiocyanate (MBT) in green timber, bacteria were isolated from freshly-felled boards of Corsican pine (Pinus nigra var. maritima Arnold) previously treated with different biocides. The...
R J Wallace, R A Eaton, G R Williams
Detection of methanogenic bacteria in mining timber
1988 - IRG/WP 1352
Methane explosions in gold mines have been attributed to spontaneous ignition of methane/air mixtures. Biogenesis of methane in the heartwood of diseased trees has been shown, but never in timber in service. Studies were therefore undertaken to establish whether methanogens were present in wooden pit props. Core samples were removed by Pressler increment borer from props in disused areas 2,600 m d...
L D Abraham, K Westlake, R I Mackie, J F Putterill, A A W Baecker.
Tunnelling bacteria
1983 - IRG/WP 1186
The micromorphology of decay in wood caused by a certain group of single-cell bacteria, called tunnelling bacteria (TB), is described. TB are characterised by their tunnelling ability within the secondary cell walls of wood fibres. Pure cultures of TB have not yet been obtained although the typical decay patterns can be reproduced using mixed bacterial cultures. Some indications of lignin degradin...
T Nilsson, G F Daniel
Microbial decay in an extremely durable Malaysian hardwood Belian (Eusideroxylon zwageri) - an overview
1997 - IRG/WP 97-10216
The heartwood of Belian (Eusideroxylon zwageri) is highly regarded among the naturally durable Malaysian hardwoods, and is therefore a choice timber for soilcontact use in the humid tropics such as untreated telecommunication poles, fence posts and railway sleepers. The high microbial resistance of the wood is evidenced by the mere superficial degradation observed in some pole samples after 20 yea...
A H H Wong, A P Singh
Ponding of radiata pine (Pinus radiata) - 2. The effect of ponding on coating penetration into wood
1998 - IRG/WP 98-10249
Radiata pine (Pinus radiata) wood panels which had been ponded for periods ranging from 2 to 12 weeks, dried and subsequently coated with a primer and examined by light microscopy after staining sections with Sudan IV to enhance the contrast of the primer material.
A correlation between the extent of ponding and the extent of coating penetration into wood was observed. The coating penetration var...
A P Singh, S S Gallagher, U Schmitt, B S Dawson, Yoon Soo Kim
The use of selective media for studying the colonization of wood in ground contact by microorganisms
1978 - IRG/WP 186
In October1976 a field trial was set up in order to monitor the progressive colonisation of wood in ground contact using orientated small stakes of Scots pine (Pinus sylvestris) and birch (Betula sp.) (30 x 45 x 250 mm³). Half the number of each species were treated with a 1% solution of CCA by a vacuum impregnation process. All the stakes were coated on three sides with an epoxy-resin sealant to...
C P Clubbe
Ultrastructure of Prumnopitys ferruginea wood from a buried forest in New Zealand
1991 - IRG/WP 1489
The buried wood of Prumnopitys ferruginea, a New Zealand native tree, was examined by scanning and transmission electron microscopy to investigate the extent to which the wood had deteriorated and the cause of its deterioration. Examinations showed that some deterioration of wood had occurred. Microscopy revealed a decay pattern similar to that produced by cavitation bacteria. Lenticular cavities ...
A P Singh, T Nilsson, G F Daniel
16S rRNA Analysis of the Bacteria Associated with Biocide Degradation
2004 - IRG/WP 04-10543
The bacterially mediated degradation of the new anti-fungal biocide, bethoxazin, was studied in vitro, by means of 16S rRNA PCR-amplification and cloning techniques. Woodblocks impregnated with a subtoxic concentration of bethoxazin were incubated in compost, and the micro-organisms associated with the wood after 6 and 12 weeks was studied. It was found that after 6 weeks the wood contained a la...
D F Wallace, D J Dickinson
Wood structure in relation to excessive absorption - a literature survey
1971 - IRG/WP 300
This literature study, composed in connection with 'the structure of wood in relation to the excessive absorption', started from the idea that the subject only could be dealt with successfully if the structure was taken into account in relation to a normal absorption pattern in so far as this knowledge can enlighten the understanding of the abnormal situation. The normal penetrat...
S M Jutte
Assessment of the effects of rentex remedial treatment on some wood pole inhabitant micro-organisms
1994 - IRG/WP 94-30053
The effects of a remedial ground-line treatment using Rentex, a stabilised paste containing a mixture of fluoride and dichromate salts, on the micro-organisms inhabiting a sample of some 160 creosoted, on-line, electricity distribution poles, have been investigated as part of an appraisal of the efficacy of this remedial treatment. Half of the poles were treated and the other half used as controls...
D C R Sinclair, G M Smith, A Bruce, H J Staines
Method of embedding and staining of wood after biological testing to support the identification of decay type
1998 - IRG/WP 98-20131
A method for preparing wood for microscopic analysis after decay testing is described, with special regard to a novel fast simultaneous staining, which helps to identify different types of decay. The novel staining process is explained as well as the embedding of heavily decayed samples in polyethylene glycol (PEG) and picking up of sections by transparent adhesive tape. Special feature of the int...
A O Rapp
Occurrence and importance of various types of fungal and bacterial decay in CCA-treated horticultural pine posts in New Zealand
1984 - IRG/WP 1234
A detailed microscopical examination has been carried out on samples taken from CCA-treated pine posts exposed in horticultural soils. The following decay types were observed: white rot, brown rot, soft rot, tunnelling bacteria, cavitation bacteria, and bacterial erosion. The occurrence and importance of the various decay types between different regions, and plots within a specific region, varied ...
T Nilsson
The effect of concrete embedment on CCA treated hardwood and softwood timbers
1985 - IRG/WP 3340
Small size stakes of Eucalyptus maculata and Pinus elliottii were treated with CCA to various retentions and embedded in concrete collars containing varying amounts of a fungicide additive. During the 30 month trial period stakes were removed after 12, 18, 24 and 30 months and assessed for residual strength. Stakes which showed significant strength reductions were microscopically examined for the ...
L E Leightley, G A Willoughby
Micromorphology of oak wood degraded by brown rot fungus Coniophora puteana
2000 - IRG/WP 00-10356
To characterize the degradation of hardwood by brown rot fungus, Coniophora puteana was incubated on the wood blocks of Quercus accutissima. For comparison, the same fungus was also incubated on the pine wood blocks of Pinus densiflora. In softwood, the shape of the wood cells and thickness of the cell appeared unchanged even the loss of birefringence in the affected areas. In contrast, oak wood d...
Yoon Soo Kim, Seung-Gon Wi, Kwang-Ho Lee
Screening of bacteria, yeasts and Trichoderma isolates for antagonism toward stain and mould fungi on agar media and wood
1999 - IRG/WP 99-20159
A screening programme of soil bacteria for antagonism toward mould and wood stain fungi was undertaken on 8 types of agar-based media by application of several bacteria to each plate. The rapid screening method was subsequently verified by testing antagonism of the most promising bacteria on Scots pine sapwood. Most of the 64 bacterial showed antagonistic effects on certain media against individua...
C Payne, A Bruce
Enhanced removal of CCA from treated wood by Bacillus licheniformis in continuous culture
1997 - IRG/WP 97-50083
A gram positive, spore-forming bacterium, which was isolated from a 20-year-old Forest Service test plot of CCA-treated 2 x 4's in Madison, WI., demonstrated the ability to release copper, chromium, and arsenic from CCA-treated wood in liquid culture. CCA-treated sawdust was exposed to this organism, which has been presumptively identified as Bacillus licheniformis. Analysis of the sawdus...
C A Clausen
Cavitation bacteria
1984 - IRG/WP 1235
A form of bacterial wood degradation is described. The term "cavitation bacteria" has been coined in order to describe the discrete cavities that are formed within the wood cell walls. The bacteria observed within the cavities appeared to be polymorphic with rounded as well as filamentous forms recognised. TEM studies suggest that the bacteria produce diffusable wood-degrading enzymes. Cavitation ...
T Nilsson, A P Singh
The association of bacteria with the increased permeability of water-stored spruce wood
1973 - IRG/WP 325
A microbiological investigation was undertaken to check our earlier work on patterns of bacterial counts and pH changes in water-stored wood. A survey of industrial activity was conducted upstream from the storage site with a view to explaining the existing bacterial flora. Further experiments were carried out with spruce bolts in enclosed tanks, using lake water with its natural bacterial flora a...
J A Dunleavy, J P Moroney, S E Rossell
Exposure of preservative treated wood to terrestrial microcosms, pure cultures of fungi and in the field
1997 - IRG/WP 97-20114
Small stakes (5x10x100 mm3) of treated and untreated Pinus sylvestris sapwood were exposed in terrestrial microcosms with different soils, two taken from test fields in Sweden, one soil from a conifer forest and one garden compost soil. Stakes of the same size were also exposed to pure cultures of the brown and white rot fungi, Postia placenta and Phanerochaete chrysosporium. After exposure, weigh...
M-L Edlund
Micromorphological and chemical changes of archaeological woods from wrecked ship's timbers
1987 - IRG/WP 4136
Micromorphological and chemical alterations of sea-waterlogged woods obtained from the ship-wrecked materials which had been submerged in Yellow Sea for over 700 years were investigated. The woods were deteriorated in varying degrees by marine organisms depending on species and parts of the woods. Under the light and scanning electron microscope the morphological characteristics of deteriorated wo...
Y S Kim
CCA removal from treated wood by chemical, mechanical and microbial processing
1998 - IRG/WP 98-50101-26
Most preservative-treated wood produced and consumed in the U.S. is treated with toxic inorganic compounds containing copper, chromium, and arsenic. Because chromated copper arsenate (CCA) is fixed to the wood, treated wood has not been considered toxic or hazardous and is currently landfilled. Increasing public concern about environmental contamination from treated wood combined with increasing q...
C A Clausen, R L Smith
A study of methanogenesis and sulphate-reduction: A possible source of explosive gas mixtures in a gold mine
1989 - IRG/WP 1399
Previous work isolated anaerobic bacteria from timbers and drainage water in a gold mine. The present work identifies the predominant methanogen in the mine ecosystem as Methanobacterium bryantii and sulphate-reducing isolates as Desulphovibrio desulphuricans and Desulphotomaculum antarcticum. Laboratory studies using model systems evaluate the activity of methanogenic and sulphate-reducing bacter...
L D Abraham, J F Putterill, A A W Baecker
Preservation of basidiomycete hyphae in ancient waterlogged wood materials
1992 - IRG/WP 92-1536
Studies on waterlogged archaeological wood show that basidiomycete hyphae may persist as long as 800 years. In two pine wood samples with Phellinus pini heartrot, one from the foremast of the ship Vasa and the other from a bulwark constructed in the first part of the 12th century, numerous resin covered hyphae were observed. Hyphae with clamp connections that were associated with brown and white r...
T Nilsson, G F Daniel