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Field evaluation of alternative antisapstain chemicals
1982 - IRG/WP 3198
Seven alternative antisapstain formulations containing four different active ingredients were field tested during the summer and fall of 1981. The test was designed to evaluate the potential long term effectiveness of PQ-8 (Chapman Chemical Co.) containing copper-8-quinolinolate, Busan 30 (Buckman Laboratories) containing 2-(thiocyanomethylthio)benzothiazole, BL2398 (Buckman Laboratories) containi...
D V Plackett


The effect of Tween 80 on the growth, morphology, and enzyme secretion of Postia placenta
1990 - IRG/WP 1456
The nonionic surfactant Tween 80 (polyethylene oxide sorbitan mono-oleate) has been reported to increase enzyme production and/or secretion in bacteria and fungi. Such a procedure could greatly facilitate research into the physiology of wood-decay fungi since quantities of available enzyme are often limiting. The brown-rot fungus Postia placenta was grown in a synthetic medium supplemented with 0,...
J A Micales


Growth inhibitory effects on blue-stain fungi of applied electricity fields
1996 - IRG/WP 96-10167
Exploratory laboratory experiments on the effects of electricity on two blue stain fungi Aureobasidium pullulans and Ceratocystis piceae on wood revealed that a potential gradient of 1 V/cm corresponding to a current of 15 mA (DC), applied without interruption during a 2 week experimental period, leads to an inhibition of the growth of these fungi. Germination is somewhat more sensitive than mycel...
J Bjurman


Testing blue stain fungicides for joinery timber in combination with natural weathering
1976 - IRG/WP 268
Joinery timber in outside use becomes affected by blue-stain after a short time of exposure. This is especially the case with pine sapwood treated with unpigmented lacquer, but also with other timber species as well as different types of surface treatment. According to the basic investigations of BUTIN this is due to special types of blue-stain fungi, called "Lack-bläue" (lacquer blue-stain), in ...
H Willeitner


Fumigation of red beech in New Zealand for prevention of graystain
2000 - IRG/WP 00-10343
The discoloration of sapwood of red beech (Nothofagus fusca) after drying has been an ongoing problem which may lower value of material for certain applications (such as flooring) by as much as 50%. The dark gray stain noted after the drying and planing of lumber is not prevented by fungicide dips, and is most likely similar to the enzyme-mediated stain noted in a variety of commercial hardwoods. ...
E L Schmidt, B Kreber


Development of bluestain in commercially harvested logs in Britain
1996 - IRG/WP 96-10150
In Britain, mechanised harvesting of conifer forestry crops is now the preferred method of felling where terrain and access allows. However, use of mechanised harvesters can lead to excessive debarking, loosening of the bark and wood splintering with, on average, about a third of the bark removed from the more severely damaged logs. More bark has also been observed to be lost from logs harvested e...
A Uzunovic, J F Webber, D J Dickinson


Utilisation of carbohydrates by stain fungi in agar culture
1998 - IRG/WP 98-10248
Stain fungi are often defined by their ability to utilise the starch and free sugars found in ray parenchyma cells, and their inability to utilise other wood constituents. However, several species of stain fungi produce bore holes in wood cell walls. This suggests that enzymatic activity capable of degrading structural polysaccharides and/or lignin is associated with the growth of the appressorium...
J Snow, P Vinden, S M Read


Comparative study of blue stain resistance of various types of wood stains after artificial and natural weathering
1992 - IRG/WP 92-2411
For the determination of the protective effectiveness of a preservative treatment against blue stain in service, artificial weathering has been proposed as an alternative for the natural weathering period of 6 months in the European standard EN 152. Research on a range of products and on complete finishing systems for external joinery was conducted during 1986-1990. It revealed that the decisions ...
J Van Acker, M Stevens, M Nys


The study of blue stain found in coniferous timber in SR Slovenia (Yugoslavia)
1983 - IRG/WP 1177
The report concerns the problems connected with blue stain in coniferous timbers stored in warehouses at wood-processing plants in SR Slovenia (Yugoslavia). The amounts of spruce-wood (Picea abies Karst.) found to have been infected with blue stain have been measured and some of the fungi causing the stain have been identified and their biology studied. It was found that the percentage of spruce-w...
R Benko


Effects of pre-harvest girdling on selected properties of red pine, red maple and Eastern larch
1999 - IRG/WP 99-40137
Trees of three species - tamarack (Larix laricina), soft maple (Acer rubrum) and red pine (Pinus resinosa) - were girdled immediately below the crown,one to two years before felling. The wood from these trees was compared with wood taken from un-girdled control trees felled at the same. Sapwood and heartwood, from the controls, and from above and below the girdle of treated trees, were examined fo...
A Taylor, P A Cooper


Extracellular carbohydrate production by isolates of Postia (=Poria) placenta
1989 - IRG/WP 1388
A monokaryotic strain of Postia (=Poria) placenta, ME20, which is unable to degrade wood, also failed to produce extracellular polysaccharide when grown in liquid culture, regardless of carbon source or concentration. Other isolates of Postia placenta, including another monokaryon and a hybrid of this monokaryon with ME20, produced large quantities of this material. The polysaccharide consisted pr...
J A Micales, A L Richter, T L Highley


Mycoparasitism by some white rot fungi on blue stain fungi in culture
1986 - IRG/WP 1304
When studying biological control of blue stain an interesting phenomenon of parasitism by some white rot fungi on blue stain fungi has been encountered. The majority of the 22 tested white rot fungi act parasitically upon blue stain fungi. The most interesting species among them is Bjerkandera adusta (Willd. ex Fr.) Karst., causing almost complete or complete decoloration of the hyphae of the blue...
R Benko, B Henningsson


Pretreatment decay in poles of Pinus sylvestris
1987 - IRG/WP 1329
Storage damage by blue stain and decay in poles before impregnation has been increasingly observed during recent years and there have been reports that salt-impregnated poles today are of lower quality because of incipient decay being increasingly common. The attacks may have started at the time of cutting and then continued during the period of seasoning at the plant, which could be as long as tw...
H Lundström, M-L Edlund


Characterization and differentiation of wood rotting fungi by protein and enzyme patterns
1999 - IRG/WP 99-20177
Standardized tests for wood preservatives are performed with defined fungal strains to ensure comparability between laboratories. However, changes of virulence and variation of results are well known events. Suitable and reliable measures to control the stability of the test organisms are necessary.Comparison of protein patterns produced by SDS-electrophoresis was already described by several auth...
U Schoknecht


Methods of testing anti-stain chemicals for protecting sawn timber during storage and transport
1976 - IRG/WP 273
No international standard method for testing anti-stain chemicals for protecting fresh sawn timber exists. The methods used can be divided into three types: a) Rapid screening tests for finding chemicals effective against blue-stain and mould fungi. The chemicals are tested in agar medium, in pieces of filter paper or on small pieces of wood using pure cultures. b) Laboratory tests for evaluating ...
T Vihavainen


Analysis of the degradation of carbohydrates by blue-stain fungi
1990 - IRG/WP 1457
The topic of the degradative ability of blue-stain fungi literature is not clear. Many authors support the idea that those fungi don't have enzymatic capability to decay the wall of the ligneous cells. However, others have found some decay activity. In this study, we have attempted to analyze which wall cells soluble carbohydrates are metabolized by some blue-stain fungi, found in Spain. ...
M T De Troya, A M Navarrete, E Relano


Chitin synthetase activity in cellular fractions of wood-decay fungi
1992 - IRG/WP 92-1524
This study was an attempt to explain the variability of in vivo sensitivity of wood decay fungi to the chitin synthetase (CS) inhibitor, polyoxin, by comparing CS distribution in cellular fractions. The five species studied were the brown-rot fungi Coniophora puteana, Gloeophyllum trabeum, Fibuloporia vaillantii, and the white-rot fungi Schizophyllum commune and Ganoderma applanatum. The CS activi...
B R Johnson, S C Croan, B Illman


Comparison of bluestain fungi growing in vitro and in vivo
1996 - IRG/WP 96-10149
Both moulds and bluestain fungi cause serious economic losses for forestry and timber processing industries and much research is aimed at finding environmentally and economically acceptable methods of control. It is especially important to study the growth of these fungi in freshly cut wood, which has been unaltered by drying or sterilisation, and which therefore resembles the substratum they woul...
A Uzunovic, J F Webber, D J Dickinson


Protection of pine sawtimber and sawn timber against blue stain in Poland
1983 - IRG/WP 3193
The purpose of this paper is the synthesis of more important results of basic and applied research that have been grouped in the following problems: 1.: Fungi causing blue stain in the pine wood, occurring in Poland. 2.: Moisture content changes of pine sawtimber and sawn timber as the factor of blue stain producing fungi development. 3.: Effect of sapwood moisture content on the degree of wood st...
E Tarocinski, M H Zielinski


Sawmill Evaluation of a Bioprotectant against Moulds, Stain and Decay on Green Lumber
2003 - IRG/WP 03-10460
Moulds, stain and decay cause serious problems on wood utilization. Though a growing volume of wood is kiln-dried, the market for green exports remains significant, and environment-friendly wood protection is required to replace traditional chemicals. As a solution to this problem, Forintek Canada Corp. developed a bioprotectant for protecting logs and green lumber from moulds, stain and decay, wh...
Dian-Qing Yang, M Gignac, M-C Bisson


Use of the enzyme-linked immunoassay (ELISA) and immunocytochemistry to detect soft rot in preservative treated and untreated wood
1990 - IRG/WP 2347
A polyclonal antibody was raised against mycelial extracts from the soft rot fungus Phialophora mutabilis. In ELISA assays, the antibody showed strong reactions with its own antigen and strong to moderate cross reactions with 6 other Phialophora soft rot species. With the exception of Ceratocystis albida, the antibody gave only weak or negative reactions with 11 other mold, blue stain and rot fung...
G F Daniel, T Nilsson


Blue stain in timber in service. Progress review of Sub-group collaborative tests - 1981/82
1982 - IRG/WP 2178
Following from the discussions after reporting Document IRG/WP/2146 at the Meeting in Sarajevo, 1981, it was decided that 1.) more precise definition was needed of the artificial weathering schedules used in the work - participants to re-check Table 8 and send further details or amendments to Dr Bravery; 2.) a further test employing a wider range of chemicals was necessary using a smaller range of...
A F Bravery, D J Dickinson


Blue Staining of Coated Modified and Unmodified Wood
2006 - IRG/WP 06-10589
We investigated resistance of unmodified, DMDHEU modified and oil heat treated uncoated and coated Scots pine wood against blue stain fungi. Both EN 152-1 and the reverse exposure tests showed excellent resistance of DMDHEU and especially of oil heat treated wood against blue staining. It is not needed to add any additional protective coating layers to prevent modified wood from staining. However,...
M Petric, M Pavlic, M Humar, M Tomažic


Direct analysis from wood of the blue stain fungi Aureobasidium pullulans and Hormonema dematioides by denaturing gradient gel electrophoresis
2006 - IRG/WP 06-10595
Aureobasidium pullulans and Hormonema dematioides are the two organisms used in the EN 152 laboratory method for determining the effectiveness of preservatives against blue stain in service. The literature concerning the disfigurement of surface coatings and exposed timber in-service states that A. pullulans is the dominant blue stain fungus, due to its frequent isolation from stained material. In...
M J Ray, D J Dickinson


Study of new wood preservatives from biological origin and low toxicity against wood decay organisms (fungi and termites)
2006 - IRG/WP 06-10600
Wood as an organic material is likely to be attacked by living organisms if no protecting treatment is applied to improve its durability. The biological agents, which in Spain, due to its specific climatologic, cause major damage to wood, are the stain fungi, the rot fungi and the subterranean termites. The commercialisation of the products actually used to treat wood against fungi and to treat t...
M T Troya, M J Prieto, F Rubio, D Lorenzo, J J Teixidó


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