IRG Documents Database and Compendium


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Lab and field test results for wood treated with polymeric alkylphenol polysulfide
2001 - IRG/WP 01-30268
A new wood preservative based on polymeric alkyphenol polysulfide (PXTS-A) is being evaluated in a series of laboratory and field tests and the results continue to show promise. A modified formulation (PXTS-B), which contains additives to reduce the viscosity, is being evaluated along with the original formulation. Soil block decay tests comparing PXTS-B with CCA-C indicate that PXTS-B is equally ...
J Goswami, A Abramson, R Buff, D D Nicholas, T Schultz


Influence of acidification on decay processes of CCB treated wood
2004 - IRG/WP 04-10514
The phenomenon of copper tolerance by brown rot fungal strains has been known for a long period but the complete mechanism of copper tolerance by these fungi is not understood yet. Copper tolerance has previously been linked to oxalic acid excreted by copper tolerant brown rot fungal strains. This acid reacts with copper in the wood to form an insoluble and therefore less toxic copper oxalate. The...
M Humar, S A Amartey, M Šentjurc, F Pohleven


An investigation of the factors influencing the rate of deterioration of timber samples due to microfungi in laboratory tests
1997 - IRG/WP 97-20116
An investigation was performed into the comparative performances of test 1 (vermiculite burial) and test 2 (soil burial) as described in ENV 807. Two test preservatives were used - copper naphthenate and propiconazole. Copper chromate was included as a reference preservative. The wood species used was beech (Fagus sylvatica). Test 1 was performed using both a mixed inoculation of the five stipulat...
I J Herring, D J Dickinson, S M Gray, J K Carey


The role of oxidation in wood degradation by brown-rot fung
1992 - IRG/WP 92-1562
Brown-rot fungi are suggested to degrade cellulose by oxidation with hydrogen radicalsformed eg. in the conversion of hydrogen peroxide in the Fenton type reactions. The stuctural changes in the holocellulose in wood induced by Fenton's reaction on wood components are very similar to those caused by brown-rot fungi. In this work the effect of the Fenton reaction on wood components was stu...
A-C Ritschkoff, J Pere, J Buchert, L Viikari


Identification of brown rot fungi on wood in above ground conditions by PCR, T-RFLP and sequencing
2004 - IRG/WP 04-10512
Fungi selected in test fields in Germany and Estonia and fungi (Coniophora puteana) cultivated on medium have been identified by the molecular methods PCR (Polymerase Chain Reaction), T-RFLP (Terminal Restricted Fragment Length Polymorphism) and sequenced to species level. The samples from the German field test showed that the fruit body sample was a different fungus than from the mycelium sample....
U Råberg, N Högberg, C J Land


Relative merits of laboratory and field tests for assessing wood decay resistance
1993 - IRG/WP 93-20009
Laboratory and field trials have long been employed for assessing natural durability of a variety of wood species, but there is a continuing debate concerning the relative merits of approach. While laboratory tests provide carefully controlled conditions for decay, they are often criticized for being too artificial to produce meaningful results. Conversely, field trials often vary widely between t...
T C Scheffer, J J Morrell


Preliminary studies of the performance of iron chelators as inhibitors of brown rot (Coniophora puteana) attack
1996 - IRG/WP 96-10185
This paper describes experiments to examine the proposal that the presence of iron is essential for brown rot fungi to utilize hydroxyl radicals remote from the hyphae as a means of converting the wood into a food source. reliminary test results are presented from trials using three different iron chelators impregnated into Scots pine (Pinus sylvestris) sapwood blocks. Their relative effects on th...
E D Suttie, R J Orsler, P M Wood


Toxic values derived from EN 113 tests - are they determined by the virulence of a test fungus? - Results from a round robin test -
1999 - IRG/WP 99-20176
The virulence of Coniophora puteana BAM Ebw. 15, the obligatory test fungus on softwood in tests according to EN 113, is known to be rather inconstant at least in some laboratories. The mass losses of untreated Scots pine sapwood blocks in an EN 113 test may range from below 20% to up to more than 50%. Possible reasons for these differences as well as the impact of a low virulence on the toxic val...
H Leithoff, R-D Peek, H V Borck, R Goettsche, H Kirk, M Grinda


Cytoplasmic and extracellular localization of manganese II dependent peroxidase(s) in white rot fungi during degradation of woody materials
1989 - IRG/WP 1416
The manner by which lignin is degraded in-situ in natural substrates by white rot fungi still remains a controversial issue particularly the distribution and role(s) played by lignin degrading enzymes (i.e. manganese II peroxidase and lignin peroxidase). In the present study, use was made of anti-manganese II peroxidase and immunolabelling techniques in conjunction with transmission electron micro...
G F Daniel, B Pettersson, T Nilsson, J Volc


Differences in pH, electrical resistance, cation composition and NIR spectra of red spruce wood during early stages of brown rot degradation
2002 - IRG/WP 02-10449
Red spruce sapwood was exposed to degradation by the brown rot fungi Coniophora puteana, Postia placenta, Gloeophyllum trabeum and Serpula lacrymans for 0, 1, 2 or 3 weeks using a modified soil block assay design. Average weight losses over time ranged from 0-8.9% during this time period. Detectable changes in pH, electrical resistance and cation compostion were observed in the wood as early as 1 ...
J Jellison, S Kelley, B Goodell, D Hui, A Ostrofsky


Preservation of wood-based panels against fungi and insects and and testing its efficiency
1976 - IRG/WP 270
Wood-based panel products which are made of susceptible wood species may be destroyed by fungi under wet conditions and by termites. The glues do not provide sufficient protection unless very high concentrations are applied. Particle boards and fibre boards are not susceptible to beetle infestation, although some species may attack plywood. Various types of preservatives provide sufficient protect...
G Becker, M Gersonde


Micromorphology of decay in Keruing heartwood by the basidiomycetes Phellinus contiguus and Dacrymyces stillatus
2002 - IRG/WP 02-10454
This study aimed to characterise the micromorphology of fungal decay in a wooden handrailing in an above ground exposure at the Thames embankment in London. Keruing heartwood (Dipterocarpus spp.) was determined as the construction timber and the two basidiomycetes Phellinus contiguus and Dacrymyces stillatus identified as main decay organisms. Their decay patterns within Keruing heartwood were...
G Kleist, M Ray, R J Murphy


European standardization for wood preservation
1991 - IRG/WP 2365
Since the last IRG 21 conference in New-Zealand, there one meeting of the plenary committee and several meetings of working groups. The interprative documents prepared by CCE for expliciting the essential requirements of the CCE Directive on the construction products and specially: mechanical resistance and stability / hygiene, health and environment / safety in use are waited to valid the progra...
Anonymous


Synergistic combination of an antioxidant and wood preservative: a preliminary study
1998 - IRG/WP 98-30172
We previously proposed that extractives in highly durable angiosperm heartwood may protect wood against white-rot fungal colonization and subsequent degradation by a dual mechanism: extractives have some fungicidal activity and are also free radical scavengers (antioxidants) and thus interfere with the fungal free radical degradative mechanisms. We tested this hypothesis using the commercial bioci...
T Schultz, D D Nicholas, J Minn, K D McMurtrey, T H Fisher


The high decay resistance in the sapwood of the naturally durable Malaysian hardwood Belian (Eusideroxylon zwageri)
2001 - IRG/WP 01-10410
It has long been assumed that the observed natural durability of the heartwood in certain timbers is perhaps associated with a relatively lower decay susceptibility also of the sapwood of these species. While the heartwood of Belian is reputedly highly decay resistant among the tropical hardwoods of Southeast Asia, laboratory decay tests reported in this paper have also confirmed the high decay re...
A A H Wong, A P Singh


Regulation of hyphal sheath formation iron-chelator production by the brown rot fungi Gloeophyllum trabeum and Postia placenta
1994 - IRG/WP 94-10074
Hyphal sheath formation and iron-chelator production by liquid cultures of the brown rot fungi Gloeophyllum trabeum and Postia placenta were monitored over time. The effects of incubation at various temperatures, pH's and concentrations of iron, manganese and nitrogen were determined. Hyphal sheath production was observed microscopically and quantified with JAVA video analysis. The hyphal...
Y Chen, J Jellison


Microscopic analysis of southern pine utility pole core segments
1992 - IRG/WP 92-1542
Fifteen macroscopic core descriptive categories were evaluated by microscopic analysis for accuracy in detecting decay and judging decay severity. Five categories were good indicators of decay as decay was detected in 28 of 48 samples. Five categories were possible indicators of early decay as decay was detected in 6 of 40 core segments and an additional 18 contained hyphae. The remaining five cat...
S E Anagnost, C J K Wang, M Wright


The Copper Tolerance of Mycelium vs. Spores for Two Brown Rot Fungi
2002 - IRG/WP 02-10422
The copper tolerance of two brown rot fungi, Gloeophyllum sepiarium and Oligoporus placentus, has been tested using both mycelia and basidiospores as inoculum sources. Mycelial growth of O. placentus on agar medium was shown to be much more tolerant to copper (as copper sulfate) than that of G. sepiarium. However, there was no difference in copper tolerance of basidiospores between the two specie...
S Choi, J N R Ruddick, P I Morris


Development of a disinfection treatment for oak logs to be imported from the USA
1984 - IRG/WP 3283
The European veneer industry depends greatly on oak supplies from the USA. To prevent the accidental introduction of the American Oak Wilt Disease (Ceratocystis fagacearum) into the member states of the EC, a disinfection treatment was developed for oak logs under consideration of the technical requirements of veneer production. Laboratory experiments and field trials in Germany and in the USA sho...
W Liese, M M Ruetze


Suitability of cotton strip testing as a screening method for the development of wood preservative formulations
2003 - IRG/WP 03-20270
This paper outlines a simple and rapid test method for screening the efficacy of wood preservative formulations against microbiological attack using cotton strips. The method was evaluated against soft rot and was found to provide reliable information on the protection of lignocellulosic material against microbiological attack. The assessment is based on visual interpretation of decay and on a sim...
H Leithoff, I Stephan, H Härtner


Organic nitrogen in wood: Growth substrates for a sapstain fungus
1993 - IRG/WP 93-10019
The nitrogen content of mature wood from lodgepole pine was 0.046% as determined by micro-Kjeldahl. Low molecular weight proteins between 14 and 21 kDa were identified in mature wood, branch wood and sap pressed from wood chips. Amino acids were quantified in acid-hydrolyzed wood samples and accounted for between 19 and 35% of the nitrogen in wood. The sapstaining ascomycete Ophiostoma piceae stra...
L D Abraham, C Breuil


Mechanistic aspects of the reactions of copper complexes with lignin in the presence of hydrogen peroxide and lipid hydroperoxide model compounds as proposed for white rot fungi
2001 - IRG/WP 01-10399
The structural principles and the oxidative reactions of the catalytic system consisting of copper, hydroperoxides, and copper coordination compounds that contain aromatic nitrogen atoms are similar to those of catalytic centers of oxidative enzymes involved in delignification reactions of white rot fungi. The system is capable of selectively degrading lignin and its low molecular weight would all...
K Fackler, P Lamaipis, E Srebotnik, M Humar, C Tavzes, F Pohleven, M Sentjurc, T Watanabe, K Messner


Developments in unsterile soil soft rot testing
1984 - IRG/WP 2210
Two unsterile soil test methods, the soil burial technique and a method using vermiculite burial with soil inoculation, were compared using different soils to determine their effectiveness in evaluation of wood preservatives against soft rot. Isolation of fungi and microscopy of decayed wood indicated that both methods produced accelerated soft rot attack in treated and untreated birch. Vermiculit...
P A McKaig


Collaborative soft rot tests: Tests conducted at Princes Risborough and Delft on soil samples from collaborating laboratories
1972 - IRG/WP 210
The laboratories which took part in the original collaborative soft rot test (Savory and Bravery, Holz u Org 5 (1) 58-90, 1970) sent samples of the soil used in the original test to Princes Risborough. Portions of this material were sent to Dr Hof for soil analyses at TNO and the remainder was employed at Princes Risborough for the conduct of comparative tests of soil virulence when used as a comb...
J G Savory


Extracellular hydrogen peroxide-producing and one-electron oxidation system of brown-rot fungi
1990 - IRG/WP 1445
Wood-component-degrading compounds involved in the initial degradation of the cellulose and lignin in wood were isolated from wood-containing culture of brown-rot fungi, Gloeophyllum trabeum and Tyromyces palustris and partially purified by gel filtration on Sephadex G-25 and DEAE-Sepharose ion-exchange chromatography. The compounds were glycoproteins. The molecular weights of the glycoproteins as...
A Enoki, S Yoshioka, H Tanaka, G Fuse


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