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Effect of wood species on decomposition efficiency of metham sodium
1992 - IRG/WP 92-3699
The effect of wood species and temperature on efficiency of metham sodium (32.1% sodium N-methyldithiocarbamate) decomposition to methylisothiocyanate (MITC) was investigated on 10 hardwoods and 9 conifers over a 144 hour period. Gas chromatographic analyses of headspace samples and ethyl acetate extracts of the wood revealed that decomposition never approached the theoretical decomposition effici...
J J Morrell


Immunolocalization of xylanase produced by Clostridium xylanolyticum Rogers and Baecker during anaerobic decay of Pinus patula
1991 - IRG/WP 1505
The xylanolytic fraction from a 72h culture of Clostridium xylanolyticum ROGERS and BAECKER was separated using non-denaturing PAGE and then used to raise polyvalent antisera in rabbits. Antibodies were purified using an affinity gel column and coniugated with colloidal gold. Pinus patula was exposed to axenic cultures of Clostridium xylanolyticum for 3 weeks, fixed, embedded and converted to ultr...
A A W Baecker, G M Rogers


The effect of extent and location of decay on strength loss in constructional timber
1994 - IRG/WP 94-30039
Radiata pine heartwood billets, 50 x 100 x 1000 mm³ were end-sealed and treated with copper-chrome-arsenate preservative (NZ Type I) to a range of retentions and penetration patterns. Cross-sections, 10 mm thick, were cut from the centre of each billet and used for determination of preservative distribution, for preservative analysis and for decay tests. The decay test consisted of laying samples...
M E Hedley, H Bier


Preservative effect of cellulose insulation material against some mould fungi and brown rot fungus Coniophora puteana in pine sapwood
1991 - IRG/WP 1484
The influence of mineral wool and loose-fill cellulose wool on biodeterioration of pine sapwood was studied. The test fungi were mould fungi Aspergillus versicolor, Cladosporium sphaerospermum, Penicillium sp. and Aureobasidium pullullans and brown rot fungus Coniophora puteana. Cellulose wool inhibited the growth of mould fungi on the face of wood contacted with insulation material at RH of 97 an...
H Viitanen


Unsuitability of the limiting dose as a criterion for assessing the toxicity of preservatives to wood-destroying fungi
1974 - IRG/WP 243
In the majority of methods for investigating preservatives for wood-destroying fungi their toxicity is assessed by determining what is called the "limiting dose". This normally denotes a range of preservative solution concentrations or retentions of dry preservative in the timber, the lower value serving to indicate the amount of preservative at which is observed the expected effect, e.g. the grow...
D A Belenkov


Biochemical relationships between biodegradation of cellulose and formation of oxalic acid in brown-rot wood decay
1991 - IRG/WP 1472
Non-enzymic hydrolysis of cellulose with low concentrations of oxalic acid was examined. The incubation of pine wood pulp with 1% oxalic acid (pH 1.3) at 35°C for 4 weeks reduced the original viscosity to 60%. Reducing sugars were liberated from various cellulosic samples by the oxalic acid treatment. However, crystallinities of cellulose in those samples did not change before and after the treat...
M Shimada, Y Akamatsu, A Ohta, M Takahashi


Accelerated decay tests on DNBP-treated Pinus patula and Eucalyptus grandis
1987 - IRG/WP 3441
Blocks (10 x 10 x 5 mm³) of Pinus patula and Eucalyptus grandis were impregnated to 0, 1.5, 3.0 and 4.5 kg/m³ 2-sec-butyl, 4, 6 dinitrophenol (DNBP). They were monitored microscopically and for weight losses over 16 weeks against monocultures of Coriolus versicolor and Coniophora puteana. Treated ministakes (10 x 10 x 100 mm³) and blocks in soil contact were similarly monitored to determine wei...
L D Abraham, A A W Baecker


Biocidal screening method of wood extractives by a direct use of cellulose TLC plate
2001 - IRG/WP 01-20226
Most of the methods to evaluate the biological durability of woods have focused on decay fungi and termite as deteriorating organisms and it is well known that one of the most important factors affecting the biological characteristics of wood is extractives. Bioassays for evaluating the biological activities of wood extractives have been mainly conducted with treated filter papers for termites or ...
R Yusiasih, T Yoshimura, T Umezawa, Y Imamura


Decay and termite durabilities of heat-treated wood
2004 - IRG/WP 04-40272
Decay and termite resistances of Plato-treated timbers were evaluated using a modified JIS decay test method, feeding test in a laboratory scale and a field exposure against Reticulitermes speratus (Kolbe) or Coptotermes formosanus Shiraki. The results showed that no significant decay durability of the treated timbers although mass loss from decay slightly decreased in case of treated timbers. ...
S Doi, K Hanataa, E Kamonji, Yuuji Miyazaki


Analysis of D-glucose metabolism of wood decay fungi using 13C-NMR and 13C-labeled substrates
2003 - IRG/WP 03-10475
D-Glucose metabolism is thought to be important during wood decay by fungi, not only for anabolic and catabolic purposes of central metabolism, but also as a potential source of peroxide required by extracellular peroxidases. There has been some confusion in the literature as to whether this peroxide-generating activity is of the glucose 1-oxidase or pyranose 2-oxidase (glucose 2-oxidase) type wi...
T H de Koker, M D Mozuch, P J Kersten


L-joint trials: Part 3: Relative performance of a range of preservative products
2002 - IRG/WP 02-30292
Long-term trials using the L-joints described in BS EN 330 and AWPA Standard E9-97 have been in progress at BRE since 1982. This paper records the current assessments of decay of L-joints in trials started between 1982 and 1994 with treatments applied to both Scots pine sapwood (Pinus sylvestris) and spruce (Picea sp.). Data are provided on a range of active ingredients including TnBTO, PCP, a...
J K Carey


Effect of mini-block test conditions on activity of Coniophora puteana
2000 - IRG/WP 00-20184
The influence of various factors of the mini-block test method on growth and activity of different strains of Coniophora puteana was studied. Decay rates of spruce samples in Petri dishes of different depths, at different wood fibre orientations and at various distances between growth medium and wood were compared. In deep Petri dishes, there were no significant differences between activity of the...
F Pohleven, M Petric, J Zupin


Effect of treating process on efficacy of CCA in a laboratory decay test
1990 - IRG/WP 3628
Test samples of Pinus radiata sapwood measuring 40x40x500 mm³ were treated with a range of concentrations of the copper-chrome-arsenate (CCA) formulation "Tanalith C" using Rueping (empty cell), Lowry (empty cell) and Bethell (full cell) treatment processes. Samples were then reduced to 40x40x7 mm³ test blocks and exposed to the decay fungus Coniophora puteana using an agar/block technique. Repl...
M E Hedley, K Nasheri, J G Van der Waals


Statistical evaluation of 'micro-bending' samples for classification of wood attacking fungi
1992 - IRG/WP 92-1544
Micro-specimens from both hardwood and softwood were exposed to selected species of field isolated fungi. The strength reduction and weight loss caused by these fungi were determined. A statistical procedure was then developed to classify the fungi into brown-, white-rot, or non-decay fungal categories based on these data. The procedure reveals the extent of hazard a fungus poses to wood members. ...
B Goodell, Jing Liu, A Homola, J Jellison, J Shottafer


Durability of five wood species against Coriolus versicolor
1992 - IRG/WP 92-1578
The durability of following five wood species Zelkova carpinifolia, Ulmus glabra, Carpinus betulus, Acer trauvetteri and Pterocarya fraxinifolia were studied against Coriolus versicolor under laboratory conditions according to DIN 52176 standards. Zelkova caprinifolia shows a reduction in weight of 2.19%, Ulmus glabra 41.69%, Carpinus betulus 45.64%, Acer trauvetteri 53.05%, and Pterocarya fraxini...
S M Kazemi


An Australian test of wood preservatives. Part 1: Preservatives, principles and practices
1978 - IRG/WP 2123
Between November, 1963, and July, 1964, a graveyard test of some 6000 preservative-treated stakes was installed at 8 sites equally distributed between Papua New Guinea in the tropics, through Queensland and New South Wales to Victoria. More than 40 different preservatives and preservative mixtures, mostly at several different levels of retention, were used to impregnate sawn specimens of Pinus rad...
J Beesley


SEM investigation of the production of extracellular mucilaginous material (ECM) by some wood inhabiting and wood decay fungi when grown on inert surfaces
1997 - IRG/WP 97-10194
A range of wood inhabiting and wood decay fungi have been studied for their production of extracellular mucilage when grown on inert surfaces, for comparison with their growth on wood. The species tested were: Coniophora puteana, Coriolus versicolor, Chaetomium globosum, Trichoderma harzianum, Ceratocystis coerulescens, Ophiostoma piceae, Mucor sp. and Penicillium sp. They were grown on plastic me...
A R Abu, R J Murphy, D J Dickinson


The practice of using concrete on wood piling for marine use in Thailand
1982 - IRG/WP 492
The practice of using concrete on wooden poles has been carried on in Thailand for a long time in pile-houses and pier constructions which have been situated in, or partly in the sea. In such instances, the hewed round and/or square-sawn heartwood poles of naturally durable timber species have been coated with concrete of about 5 to 10 cm or more in thickness, and to about 100 cm above the highest...
B Anuwongse


Proposals for further co-operative studies on determining toxic values against wood-destroying Basidiomycetes
1985 - IRG/WP 2247
Document IRG/WP/2194 reported the status of the programme of co-operative experiments up to and including December 1982. During discussions at IRG-13 in Turkey members endorsed the decision to complete the programme of tests and conduct repeat tests. At IRG-14 in Brisbane the report was noted but no further action agreed. At IRG-15 in Sweden a number of interested parties in the European/Scandinav...
A F Bravery


Oxalic acid quantification, oxaloacetase assay and ESI localization of P, C, and Fe from the brown rot fungus Postia placenta
1994 - IRG/WP 94-10063
The mechanism by which brown-rot fungi initiate depolymerization of holocellulose in wood remains unknown. Recently, oxalic acid (OA) has received considerable attention in cellulose breakdown by brown-rot fungi. The OA could serve as a proton donor for hydrolytic or an electron donor for oxidative (Fenton's reaction-H2O2/Fe2+) cleavages of cellulose. The acid may originate via oxaloaceta...
C R Jordan, W V Dashek, T L Highley


Report on the co-operative leaching tests
1975 - IRG/WP 246
The Work reported here was undertaken at the request of CEN to see (1) whether the standard beaker leaching method under discussion at CEN, and based on the German DIN could be made less labour intensive; and (2) whether the leaching parameters could be expressed in more general terms so that alternative procedures could be used. A co-operative experiment was set up in which the amount of leaching...
J W W Morgan


Limiting Conditions for Decay in Wood Systems
2002 - IRG/WP 02-10421
Hygrothermal models can predict temperature and moisture conditions in wall components subjected to real weather data, but specific data and a fundamental understanding of how temperature and wood moisture content dictate the progression of decay under these conditions is required for modellers to predict consequences of decay on building performance. It is well understood that wood will decay abo...
P I Morris, J E Winandy


Proposed model for the penetration and decay of wood by the hyphal sheath of the brown-rot fungus Postia placenta
1989 - IRG/WP 1391
Scanning electron microscopy (SEM) of Pinus sp. decayed by the brown-rot fungus Postia placenta confirmed the existence of extracellular membranous structures previously described by transmission electron microscopy (TEM). These structures appear to be an integral part of the hyphal sheath and assume a variety of forms including lamellar sheets, fibrils, and vesicles. These structures were observe...
F Green III, M J Larsen, L L Murmanis, T L Highley


Early detection of brown-rot decay in southern yellow pine using immunodiagnostic procedures
1990 - IRG/WP 2356
Immunodiagnostic procedures have been used to detect incipient decay of southern yellow pine by six common brown-rot fungi. Rabbit polyclonal antibodies were raised to liquid culture fractions of the six fungi. The antibodies, after preadsorption to sawdust, were tested in particle agglutination assays, immuno-dot blot, and ELISA for their·ability to detect the decay organisms when they were grow...
C A Clausen, F Green III, T L Highley


A study of the colonization of wood blocks in a laboratory unsterile soil test
1988 - IRG/WP 2318
CCA treated and untreated beech blocks were exposed to a defined horticultural loam using the method proposed for the collaborative soft rot test in the soft rot sub-group of Working Group Two. At intervals during the incubation wood samples were removed and fungal isolations were made using selective media. Fungi were identified and tested for their cellulolytic ability and their decay capacity i...
M T De Troya, S M Gray, D J Dickinson


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