IRG Documents Database and Compendium


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Pretreatment decay in air-seasoning Scots and Corsican pine poles in England
1989 - IRG/WP 1390
British grown Scots and Corsican pine poles were sampled for colonization by basidiomycetes after air-seasoning for 6 to 9, or 16 to 20 months. The amount of infection present in these poles was compared with that in poles which were imported from Finland. After 6 to 9 months seasoning, the British grown pine poles were heavily infected with Peniophora gigantea, Cylindrobasidium evolvens, Stereum ...
A R Zahora, D J Dickinson


The effect of of sorbitol on the decay of boric acid treated Scots pine
1991 - IRG/WP 1509
The tetra-hydroxy borate ion is known to undergo complexation with polyols of biological importance and probably protects treated timber by this action. The inhibitory effect of boric acid upon fungal growth and dehydrogenases has been shown to be prevented by the addition of certain polyols. This work was extended, with a study of the effects of a polyol on the performance of boric acid in treate...
J D Lloyd, D J Dickinson, R J Murphy


Field studies investigating the efficacy of biological treatments in preventing decay of freshly-felled pine
1993 - IRG/WP 93-10022
Four species of fungi (Trichoderma viride, Ascocoryne sarcoides, Potebniamyces conifererum and Cryptosporiopsis terraconensis) and sterile distilled water were applied separately to unpeeled, freshly-felled pine billets. The fungi were all in the form of concentrated aqueous spore suspensions, and were spread onto the end-grains of the logs by brush. Destructive sampling of the billets was carried...
M W Schoeman, D J Dickinson


Comparison of two laboratory methods for screening potential anti-sapstain chemicals
1993 - IRG/WP 93-10036
Two methods were used to screen a number of potential anti-sapstain chemicals in the laboratory. In one method miniature boards of freshly felled Scots pine sapwood were dip treated with the candidate chemical, sprayed with a suspension of stainers or moulds and incubated for five weeks. In the other method antibiotic assay discs were treated with precise quantities of chemical, plated out on malt...
S M Gray, D J Dickinson


The effect of initial moisture content on wood decay at different levels of gaseous oxygen concentration
1999 - IRG/WP 99-10316
The influence of initial moisture content on wood decay at different levels of atmospheric oxygen concentration was studied. Three fungi, Coriolus versicolor a white rot, Coniophora puteana a brown rot, and Chaetomium globosum a soft rot, were chosen. The mycelia of the fungi were inoculated on mini blocks of Fagus sylvatica (beech) and Pinus sylvestris (Scots pine). Incubations were under four le...
S M Kazemi, D J Dickinson, R J Murphy


16S rRNA Analysis of the Bacteria Associated with Biocide Degradation
2004 - IRG/WP 04-10543
The bacterially mediated degradation of the new anti-fungal biocide, bethoxazin, was studied in vitro, by means of 16S rRNA PCR-amplification and cloning techniques. Woodblocks impregnated with a subtoxic concentration of bethoxazin were incubated in compost, and the micro-organisms associated with the wood after 6 and 12 weeks was studied. It was found that after 6 weeks the wood contained a la...
D F Wallace, D J Dickinson


Extracellular mucilage (ECM) in wood decay basidiomycetes
2002 - IRG/WP 02-10439
The ability of wood decay basidiomycetes to produce extracellular mucilage (ECM) and its relationship with total biomass production is being investigated. Growth and ECM production by the brown-rot fungus Gloeophyllum trabeum (FPRL 108 N) and the white-rot fungus Coriolus versicolor (CTB 863 A) was assessed in liquid culture under different conditions and in the presence of the fungicide cyprocona...
D Vesentini, D J Dickinson, R J Murphy


Aureobasidium or Hormonema? A Genetic Approach.
2004 - IRG/WP 04-10529
Aureobasidium pullulans is the main organism causing disfigurement of coatings on wood and the surface of exposed timber. This disfigurement of timber in-service is referred to as “bluestain in-service”. A. pullulans is also associated with the sapstaining of dead wood in the forest and in-service. A. pullulans is noted for its highly variable growth forms (polymorphisms). This variability pre...
M J Ray, D J Dickinson, M Buck


The Bacterial Biotransformation of IPBC
2002 - IRG/WP 02-10437
The bacterial biotransformation of the biocide IPBC, widely used as an antisapstain wood preservative, was investigated in bacteria isolated from failed wood, soil experiencing IPBC wash-off, and John Innes No. 2 compost. Nine strains of bacteria were isolated, belonging to the genera Alcaligenes, Enterobacter, Microbacterium and Pseudomonas. The sole organic degradation product in an enteric bact...
S R Cook, J Sullivan, D J Dickinson


Preliminary results from the field experiment to determine the performance of preservative treated hardwoods with particular reference to soft rot. The four reference timber
1980 - IRG/WP 3164
The results given in the Tables 1-4 each refer to one of the four reference species treated with four solution concentrations of CCA as recorded from each test site. The species are: Alstonia scholaris, Betula pendula, Fagus sylvatica, Pinus sylvestris. The treatments were: Untreated, 0.66% CCA, 1.53% CCA, 3.01% CCA, 5.60% CCA. Each figure is an average of the ratings recorded for each replicate o...
J F Levy, D J Dickinson


Hardwood Field Experiment: Progress Report 1977-1986
1986 - IRG/WP 3391
The international hardwood field experiment was planned in l976 and set up in same 30 different sites around the world. The test stakes include 4 reference species common to each site and in most cases at least 2 species of local importance. It was hoped that a picture of performance of a range of economically important species would be built up and at the same time provide vital background inform...
D J Dickinson, S M Gray


Copper based water-borne preservatives: Copper adsorption in relation to performance against soft rot
1987 - IRG/WP 3452
As part of a fundamental study concerned with the performance of copper based water-borne preservatives the adsorption of copper from aqueous solutions by 4 hardwood and 1 softwood species has been studied. Experiments were conducted to determine the influence of solution pH, solution concentration and solution temperature. The results obtained were consistent with an acid form ion-exchange mechan...
P M S Rennie, S M Gray, D J Dickinson


Comparison of the inhibitory effects of borate, germanate, tellurate, arsenite and arsenate on 6-phosphogluconate dehydrogenase
1991 - IRG/WP 1508
Sodium borate inhibits the enzymatic activity of many dehydrogenases, and it is thought that this is due to the complexation of the borate anion with the coenzyme nucleotide. It has been suggested in the past that complexation of this type, leading to enzyme inhibition and other biological effects, is responsible for the inhibition of fungal growth and consequently the protection of boron treated ...
J D Lloyd, D J Dickinson


Fungal detoxification of organotin biocides
1985 - IRG/WP 1258
The ability of a range of wood decaying fungi to inactivate bis(tri-n-butyltin) oxide (TnBTO) in the extracellular growth medium, in stationary liquid culture was determined. A distinction between the ability to tolerate the fungicide and to inactivate it was made: the white-rot organism Coriolus versicolor being the most efficient inactivator. In an attempt to determine the extracellular agents r...
P S Belford, D J Dickinson


A biochemical explanation for the observed patterns of fungal decay in timber
1980 - IRG/WP 1111
Experiments designed to compare the degree of localization of the cellulase enzymes of some white, brown and soft rot organisms are described. The site and nature of binding of the enzymes is discussed. The technique is ellution of mycelium grown in liquid culture with a variety: of agents including acetate buffer, carboxymethyl cellulose solution, borate/glycerol buffer and urea. The mycelium was...
N B Green, D J Dickinson, J F Levy


The effect of acid rain on CCA treated timber
1990 - IRG/WP 3579
The effect of acid precipitation on the leaching of copper, chromium and arsenic from timber treated with different CCA preservatives was studied in the presence of three types of soil under simulated field conditions. Pine sapwood, embedded in soil, was subjected to simulated rainfall at three different levels of acidity. The CCA preservative formulations tested were:- American Wood Preservers&am...
R J Murphy, D J Dickinson


Blue stain in timber in service. Preliminary results of the IRG collaborative tests to compare natural and artificial weathering
1978 - IRG/WP 286
The collaborative programme was initiated at IRG Meetings in Wildhaus, 1976. The six collaborators were BAM (Berlin), Cuprinol Ltd (UK), EMPA (Switzerland), GORI (Denmark), PRL (UK) and Imperial College (UK). Experimental plans were finalised in December 1976 and panels treated by Cuprinol Ltd early in 1977. Natural weathering was carried out by BAM, Cuprinol, EMPA, GORI and PRL during April-Septe...
A F Bravery, D J Dickinson


Accelerated testing for out of ground contact using natural biological preconditioning
1996 - IRG/WP 96-20088
Small blocks made of Scots pine sapwood were treated, buried in vermiculite and exposed to natural microbial colonisation during outdoor exposure prior to laboratory decay testing. Periodic microbial isolations, moisture content, permeability and weight loss tests were also conducted. Bacteria and moulds colonised the vermiculite and miniblocks well. Aureobasidium pullulans was common on TnBTO tre...
S Molnar, D J Dickinson, R J Murphy


The effect of boric acid on the protozoan numbers of the subterranean termite, Reticulitermes lucifugus
1996 - IRG/WP 96-10148
Lower termites harbor in their guts populations of microorganisms known to be indispensable for their survival, being responsible, at least partially, for the digestion of cellulose, the main item of termites'diet. The Reticulitermes species in particular harbor in their hindguts some unique intestinal fauna, for instance protozoa belonging to the genera Pyrsonympha, Dinenympha and Trycho...
L Nunes, D J Dickinson


Biological control of internal decay in Scots pine poles: A seven year experiment
1992 - IRG/WP 92-1529
The interactions between key fungi were monitored, by non-destructive and destructive sampling methods, during the course of an experiment on the effectiveness of Scytalidium sp. and Trichoderma spp. for eliminating established infections of Lentinus lepideus (Fr. ex Fr.) Fr. in creosoted Pinus sylvestris poles. Although extensively isolated at the beginning and end of the experiment, Trichoderma ...
P I Morris, D J Dickinson, B Calver


The effect of copper/chrome/arsenic (CCA) treated timber on soil fungi
1981 - IRG/WP 1131
The aim of this investigation was to study the effect of CCA treated wood on the microflora of soil with particular reference to copper tolerant soft rot fungi. The IRG collaborative field experiment was designed to monitor the performance of a range of preservative treated hardwoods with particular reference to soft rot in CCA treated timber (Dickinson 1976) and site 33 of this experiment was use...
R J Murphy, D J Dickinson


Microbial ecology of treated lap-joints exposed at Hilo, Hawaii for 12 months
1996 - IRG/WP 96-20089
Lap-joints made from Southern yellow pine treated with ACQ, DDAC or Propiconazole were exposed and sampled over 12 months. TnBTO treated Lap-joints were later exposed as a standard treatment. The moisture content of the joint area remained at 23% to 28%, but, away from the joint, varied between 20% to over 30%. Untreated samples had higher moisture contents and there was little difference between ...
S Molnar, D J Dickinson, R J Murphy


Development of bluestain in commercially harvested logs in Britain
1996 - IRG/WP 96-10150
In Britain, mechanised harvesting of conifer forestry crops is now the preferred method of felling where terrain and access allows. However, use of mechanised harvesters can lead to excessive debarking, loosening of the bark and wood splintering with, on average, about a third of the bark removed from the more severely damaged logs. More bark has also been observed to be lost from logs harvested e...
A Uzunovic, J F Webber, D J Dickinson


Soft-rot testing. Memo to CEN
1983 - IRG/WP 2206 A
1. Development of a single test procedure to assess performance of preservatives against soft rot fungi is an ideal that cannot be realised at present, if results are to be both reproducible between laboratories and pertinent to the practical requirements of individual countries. 2. Consensus opinion amongst members of Sub Group I - Soft rot tests, of Working Group II - Fundamentals of Testing, of the IRG, is that the immediate needs of CEN would be best answered by: (a) a code of recommended practice, (b) a multiplicity of test procedures, and (c) a degree of flexibility in test procedures. 3. The code of recommended practice should recognise the need for: (a) a pure culture, synthetic medium (sand or vermiculite + nutrients), single wood species test to be adopted by all laboratories as a common reference point of testing, (b) mixed inoculum and/or unsterile soil laboratory or fungus cellar tests which account for local variations in the fungal flora, (c) incorporation of a range of wood species where applicable (i.e. where mixed hardwoods comprise the main resource), (d) provision of local internal standards of known performance under field conditions, (e) incorporation of leaching procedures other than standard methods with deionized water (e.g. mild acid, milk alkali, ionic solutions of various salts) where applicable to local exposure conditions, (f) precise definition of the criteria used for establishing toxic thresholds (mass loss, strength loss, microscopic examination). 4. Initial screening tests should remain the province of the individual research worker. 5. The code of recommended practice should have the sole purpose of establishing provisional retention levels for subsequent confirmation by field trial. 6. IRG should list and approve test procedures considered adequate to establish toxic thresholds for preservatives against soft rot fungi. 7. It should be clearly understood that when using a series of test techniques, it is inevitable that a range of toxic thresholds will be obtained for the same preservative. 8. Guidelines should be drawn up for interpretation of test data for subsequent transformation to provisional retention levels. 9. Adoption of the broad principles outlines above is recognition of the dynamic nature of toxic thresholds. The additional information obtained by a more flexible, and complex, approach to testing may make interpretation more difficult but should provide data from which response of preservatives to varying biological and environmental hazards can be more realistically assessed. 10. It is recommended that CEN adopt, in principle, the basic philosophy of testing outlined above and move towards formulation of a Working Document in collaboration with IRG
J A Butcher, D J Dickinson


A field study on the suitability of the European lap-joint test
2001 - IRG/WP 01-20239
Within the framework of the European SM&T Research Project "Co-normative research on field tests for wood preservatives out of ground contact in conjunction with methods for preconditioning test specimens prior to test" (FACT) one aim was to establish the suitability of the European lap-joint test (as it is described in ENV 12037:1996) to assess wood preservatives in the field in EHC 3. Scots ...
M Grinda, Ö Bergman, H-V Borck, D Dickinson, P Esser, R Gründlinger, H Leithoff, S Molnar, L Paajanen, N Pfabigan, E D Suttie, F Thomassin, T Nilsson, J Van Acker, M Van Der Zee, A Voss


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