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Gene expression of selected decay enzymes produced during biodeterioration of three wood types
2009 - IRG/WP 09-10702
Comparative studies were conducted on the expression of several decay enzyme genes during the decay of pine, cedar, and ACQ treated pine over 10 months. Measurements of MOE, decay rating, and moisture control were monitored for physical properties. Identification of decay fungi and the detection of decay enzymes were carried out before gene expression levels were measured. The MOE of wood stakes d...
Young-Min Kang, L Prewitt, S Diehl


Durability and fungal colonisation patterns in wood samples after six years in soil contact evaluated with qPCR, microscopy, TGA, chitin- and ergosterol assays
2009 - IRG/WP 09-20402
There is a need to establish new objective and sensitive methods for early detection and quantification of decay fungi in wood materials. Molecular methods have proven to be a useful tool within wood protection issues, however, this field is still poorly explored and so far relatively few have used these methods within the field of wood deterioration. Among the techniques used in the indirect quan...
A Pilgård, G Alfredsen, I Børja, C Björdal


Accelerated H3 above-ground decay trials in Australia
2009 - IRG/WP 09-20411
An above-ground H3 fungal field trial was installed at five sites (3 in Australia, 2 in New Zealand), with the aim of finding a method that would shorten the time required for evaluating new preservatives and protection systems. One-quarter of the H3 preservative retentions were mostly installed, as first results suitable for registration occur when these reach 70% soundness. Chemical analysis of ...
L J Cookson, J Carr


Review of candidate graded particle barrier testing methods in Australian Standard (AS 3660.3 – 2000): Assessment criteria for termite management systems
2009 - IRG/WP 09-20417
The Australian Standard (AS. 3660.3 – 2000) for assessment of candidate graded particle termite barriers is reviewed and suggestions for amendments are made. Areas requiring amending in light of current biological knowledge involve the inclusion of proprietary or patented systems rather than generic types; selection of test termites in the genus Coptotermes; duration of test periods; and inadequ...
J R J French, B M Ahmed (Shiday), B L Schafer


Evaluation of Water Repellency of Treated Wood Using Several Alternative Methods
2009 - IRG/WP 09-20419
In this study, the results of water repellency testing using a digital swellometer method for water-borne organic-preservative systems with water repellent additives are reported. The findings suggest that the traditional method of using matched untreated controls as a reference developed from historic solvent-based treatments for determination anti-swelling efficacy (ASE) may underestimate the a...
L Jin, A F Preston


Analysis on dynamics of basidiomycetes in decayed wood by a method using non-specific amplification of DNA
2009 - IRG/WP 09-20426
A combination of non-specific amplification of DNA by Phi29 DNA polymerase and specific amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) by polymerase chain reaction (PCR) was applied to elucidate the ratio of basidiomycete species inhabiting decayed wood from an outdoor bench. After more than 8 hours incubation with Phi29 DNA polymerase, concentration of DNA ...
T Wada, K Igarashi, M Samejima


Protection mechanisms of modified wood against decay by white and brown rot fungi
2010 - IRG/WP 10-10713
The resistance of beech and pine wood blocks treated with 1,3-dimethylol-4,5-dihydroxyethylene urea (DMDHEU) against T. versicolor and C. puteana increased with increasing WPG. Full protection (mass loss below 3%) was reached at WPGs of approximately 15% (beech) and 10% (pine). Metabolic activity of the fungi in the wood blocks was assessed as heat or energy production determined by isothermal mic...
C Mai, P Verma, Yanjun Xie, J Dyckmans, H Militz


Assessment of antagonism between lignicolous microorganisms: Research on possible use to preserve wood poles
2010 - IRG/WP 10-10718
Biological environments contain a certain number of microbial populations which, within a given ecological niche, display various relations ranging from symbiosis to parasitism. Researchers have been interested in these types of relations for around fifty years, especially in one very particular type of relationship: the antagonism exerted between individuals of the same microbial population. Toda...
J-F Labrecque, A Zaremski, L Gastonguay, Y Prin


Structural changes, basidiomycete richness, enzyme activity and proteomic profiling of decay resistant and non-resistant woods over 18 months in soil contact
2010 - IRG/WP 10-10733
Wood decay fungi damage wood by production of enzymes that attack the structural components. The objective of this study was to better understand which suite of decay genes and proteins are expressed during biodeterioration of three different wood types in forest soil over time. Variation in decay genes and proteins were determined for pine (non-resistant), cedar (naturally durable), and ACQ-treat...
Youngmin Kang, S Diehl, L Prewitt, D Nicholas


Natural durability of African Padauk and Niove. Comparison of new trials and former data from the CIRAD database
2010 - IRG/WP 10-10743
African Padauk (Pterocarpus soyauxii) and Niove (Staudia kamerunensis) were tested in laboratory against Basidiomycetes. The results were calculated according to the requirements of EN 350-1 (1994) and XP CEN/TS 15083-1 (2006). Padauk samples showed a high durability and very small variations between trees and within tree. Niove presented a high durability, but with notable differences according ...
Y N N’Kolo Meze’e, N Leménager, C Baudassé, M-F Thévenon


Glued laminated poles - Progress report after 30 years testing
2010 - IRG/WP 10-20427
In 1979 a number of glued laminated poles treated with CCA and creosote were placed in a greenhouse at Uppsala, at the Simlångsdalen test field in southern Sweden and in a power lane in Vuollerim in northern Sweden in order to study their resistance against biological degradation. The tests have shown that the best performance will be obtained if each lamination is treated with a water-borne pre...
Ö Bergman, J Jermer


The Use of Micro-Tensile Testing to Assess Weathering Decay and Oxidative Degradation of Wooden Items
2010 - IRG/WP 10-20433
This paper presents a method to study the weathering performance of wooden items and to assess the oxidative degradation of wood via the Fenton reaction. Weathering resistance and photo-stability of wood was tested using pine wood (Pinus sylvestris) veneer strips measuring approximately 60 µm in thickness. The veneer strips were treated using a reactive chemical to impart surface protection. The ...
C Mai, Yanjun Xie, Zefang Xiao, P D Evans, H Militz


Accelerated L-joint performance testing of organosilicon treated wood
2010 - IRG/WP 10-30551
In this research Scots pine sapwood L-joints were treated with organosilicons and organosilicons in combination with biocides. These L-joints were prepared and exposed according to the accelerated L-joint method. They were inspected each season for fungal discolouration and removed from the rack after three years outdoor exposure. Subsequently the moisture content, mass loss and decaline uptake we...
L De Vetter, J Van Acker


Moisture dynamics of WPC as basis for biological durability
2010 - IRG/WP 10-40520
The largest market for wood-polymer composites (WPCs) is currently decking. Although many products are commercially available, a proper standard for the assessment of the biological durability of WPC does not exist. Recommended standards for testing resistance against basidiomycetes should be completed with a method to bring the specimens in a worst case situation, obtaining a moisture level high ...
N Defoirdt, J Van Acker, J Van den Bulcke


Testing of decay resistance of sapwood and heartwood of thermally modified Scots pine and Norway spruce
2010 - IRG/WP 10-40523
Thermal modification at elevated temperatures changes the chemical, biological and physical properties of wood. One of the main targets of thermal modification is to increase the biological durability and decay resistance of wood. Although the effects of thermal modification on wood have been widely studied, the significance of sapwood and heartwood on the resistance of thermally modified wood has...
H Viitanen, S Metsä-Kortelainen


Durability testing of coconut shell according to ENV 807
2011 - IRG/WP 11-10761
Coconut shell was tested in the laboratory according to the European standard ENV 807 with three different soil types: compost soil, brown rot/soft rot rich soil and white rot/soft rot rich soil. Mass losses between 14 and 16 % were achieved with all three soils, indicating that the decay type is of little importance in the degradation process. Somewhat higher mass losses, 19-22 % were obtained fo...
J Jermer, A H H Wong, K Segerholm, T Nilsson


Methods of field data evaluation - time versus reliability
2011 - IRG/WP 11-20466
Field testing of wood and wood-based products is so far the most reliable way to get information on performance with respect to durability and expected service life. However, field trials are extremely time and cost consuming. The main objective of the present study was to investigate possible correlations between early stages of decay, preferably during the first 5 years, and the actual service l...
P Larsson Brelid, C Brischke, A O Rapp, M Hansson, M Westin, J Jermer, A Pilgård


Above ground testing at tropical test sites, what have we learned?
2011 - IRG/WP 11-20473
Three different above ground test methods have been utilized at a selection of five tropical and sub-tropical test sites with a variety of treated and untreated material. The results show that a multi-site approach to above ground field tests presents the opportunity for exposure to un-predicted biodeterioration hazards, which may be important for developmental products of poorly characterized fu...
A Preston, A Zahora, Y Cabrera, L Jin, C Schauwecker, P Walcheski


A comparative study and evaluation of methodologies used for determining wood preservative penetration
2011 - IRG/WP 11-20475
A series of methods for determining penetration of wood preservatives into the wood structure have been developed for either quality control (QC) or research and development purposes. QC methods range from monitoring the solution uptake, applying colour indicators or sampling specific wood samples followed by wood acid digestion/atomic absorption spectroscopy (AAS) or solvent extraction/chromatogr...
R Craciun, R Moeller, J Wittenzellner, T Jakob, J Habicht


The durability of acetylated radiata pine sapwood (Accoya). Results from five years testing
2011 - IRG/WP 11-30560
New Zealand grown radiata pine sapwood was modified using the Accsys PLC acetylation process (Accoya). Following treatment, various test samples were exposed in the Scion Accelerated Field Test facility (Fungus Cellar) and in exterior ground contact field tests. In the Fungus Cellar, performance was compared with that of radiata pine treated with CCA to Hazard Classes H3.2 (0.37% m/m Cu+Cr+As) and...
M Hedley, D Page, J van der Waals, K Nasheri, G Durbin


Novel Approaches to the Development and Testing of Anti After-glow Additives for CCA-based Treatments of Poles
2011 - IRG/WP 11-30561
In this paper, a field fire stake testing screen method is described. This method uses stake samples from matched wood sources allowing replicates from different boards to be included. The method is easy to control and can provide quick comparative fire resistance test results. The parameters measured from the tests are percentage weight loss, minimum residual circumference and residual thicknes...
L Jin, P Walcheski, A Preston


Comparison of rates of wood decay from four different field test protocols following 4-5 years exposure at a site in New Zealand
2011 - IRG/WP 11-30565
The rate of decay of Pinus radiata was evaluated using four different field test protocols at a site near New Plymouth, New Zealand. The average Scheffer index was 86 over the five year period of testing. The field tests were an in-ground stake test (AWPA Std E7-01) and three above ground tests – lap-joint (AWPA Std E16-98), decking (AWPC protocol) and ground proximity tests (AWPA Std E18-06) T...
P Lobb, K Day, A Siraa


The resistance to Basidiomycetes fungi of chipboard 35 years after their industrial preservation with wood preservative on fluoroborate basis
2011 - IRG/WP 11-40546
The research on wood-based panels’ (WBP) behaviour towards fungi impact demonstrated rather low resistance of wood-based materials to wood rotting fungi. However, these materials are used in construction under conditions favourable to fungi growth. In such conditions WBP treated with wood preservatives should be used. Preservation durability is of great importance in such cases. The resistance o...
A Fojutowski, A Kropacz


Challenges and new developments in testing electro osmotic pulsing technology (PLEOT) by means of conventional laboratory methods
2011 - IRG/WP 11-40555
A new wood protection system, electro-osmotic pulsing technology, called PLEOT, is tested in lab trials using different test setup. The technology is tested on Scots pine sapwood and beech wood samples exposed to brown- and white rot fungi. Mass loss and moisture content is calculated after testing and a chitin analysis are performed on the sample powder. Two different electrodes are used in ord...
A Treu, E Larnøy


Durability of modified wood in UC3 and UC4. Results from lab tests and 5 years testing in 3 fields
2011 - IRG/WP 11-40562
In 2005 an extensive test program including field tests was set up in order to obtain more data on the durability and long term performance of modified wood and semi-durable wood species. One of the main challenges for modified wood is to predict accurate service life time in UC3 (use class 3, above ground) and UC4 (use class 4, in soil or fresh water contact). So far, data from in-service condi...
M Westin, G Alfredsen


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