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Comparative study of lignocellulolytic activities of Pleurotus spp. and white rot and brown-rot fungi
1997 - IRG/WP 97-10210
In this work, the in vitro species of Pleurotus (P.eringii, P. cornucopiae and P. ostreatus), from structures of degraded wood have been studied. This degradative activity was compared to those presented by Trametes versicolor (fungus characteristic of white rot) and Gloeophyllum trabeum (fungus representative of brown rot). The activities studied were the following: cellulase, xylanase, pectinase...
D Muñoz-Mingarro, F Llinares, M T De Troya, F Rubio, M Yuste, C Rodríguez-Borrajo, J E Garcia de los Rios, F Alvarez, P Jiménez, A Rojas, A Navarrete, P Reche


Evidence for the participation of the osmiophilic particles in the enzymatic demethylation of lignin
1984 - IRG/WP 1239
Transmission electron microscopic observations of the white-rot fungi Trametes hirsuta and Phanerochaete chrysosporium grown on a series of wood pulp with a graded lignin content and mycelium from the clarified centre of an agar-medium containing lignin (E1-effluent from the bleaching process) showed that osmiophilic particles are only induced by native lignin. Taking into account that the osmioph...
K Messner, H Stachelberger


Proposed model for the penetration and decay of wood by the hyphal sheath of the brown-rot fungus Postia placenta
1989 - IRG/WP 1391
Scanning electron microscopy (SEM) of Pinus sp. decayed by the brown-rot fungus Postia placenta confirmed the existence of extracellular membranous structures previously described by transmission electron microscopy (TEM). These structures appear to be an integral part of the hyphal sheath and assume a variety of forms including lamellar sheets, fibrils, and vesicles. These structures were observe...
F Green III, M J Larsen, L L Murmanis, T L Highley


Production of exraxellular hydrogen peroxide and oxalic acid by brown-rot fungus Poria placenta
1995 - IRG/WP 95-10112
Hydrogen peroxide and oxalic acid have been suggested to be essential in the degradation of wood carbohydrates by brown-rot fungi. Hydrogen peroxide has been suggested to be one of the diffusible low molecular weight agents produced by brown-rot fungi for the degradation of wood carbohydrates by generating highly active radicals. The production of hydrogen peroxide and oxalic acid by Poria placent...
A-C Ritschkoff, M Rättö, J Buchert, L Viikari


Early detection of brown-rot decay in southern yellow pine using immunodiagnostic procedures
1990 - IRG/WP 2356
Immunodiagnostic procedures have been used to detect incipient decay of southern yellow pine by six common brown-rot fungi. Rabbit polyclonal antibodies were raised to liquid culture fractions of the six fungi. The antibodies, after preadsorption to sawdust, were tested in particle agglutination assays, immuno-dot blot, and ELISA for their·ability to detect the decay organisms when they were grow...
C A Clausen, F Green III, T L Highley


Degradation of lignin model compounds with coordinated copper in the presence of peroxide
1998 - IRG/WP 98-10282
A novel diffusible system capable of decomposing lignin model compounds with copper, coordinators and peroxides has been proposed. When pyridine was used as a copper coordinator, two synthetic dyes, Poly-R and RBBR were intensively decolorized with Cu(II) and H2O2 in aqueous solutions at room temperature. Although reactions with Cu(II) and H2O2 produce a strong oxidant, hydroxyl radical, via a Fen...
K Koller, K Messner, T Watanabe


Detection of a brown-rot fungus using serological assays
1986 - IRG/WP 1305
Polyclonal antisera produced to Poria placenta (Fr.) Cooke were used in two tests to qualitatively assay for the fungus. Fungal hyphae were fixed to slides and fluorescent antibody (FA) techniques used to visualize the hyphae under the microscope. Fluorescence of non-Poria fungi, when present, could be reduced but not eliminated by cross-absorbing the sera with these fungi. The antisera was also u...
B Goodell, J Jellison


An evaluation of chemical pre-treatments for controlling kiln brown stain in radiata pine
1997 - IRG/WP 97-30124
Clear radiata pine (Pinus radiata D. Don) is widely used for fine millwork owing to its attractive light colour. However, kiln drying of radiata pine sapwood often results in the development of a brown stain causing substantial loss in revenues in New Zealand's high-value export markets. In this study chemical pre-treatments were evaluated for their ability to control the formation of kil...
B Kreber, A McDonald


Preservative ability of wood to be fixed hydroxyl apatite substituted for antimicrobial metals
2001 - IRG/WP 01-30272
We succeeded in forming hydroxy apatite (HAp) in wood. HAp is non-toxicity and safe. Preservative ability of the wood, which substituted one part of Ca of constituent element of this HA p for antibacterial metals was measured. When Ca was substituted for Ag or Zn, mass loss in decay by brown-rot fungus F. palustris was restrained in about 50% (Ag) ~30% (Zn) of value of control specimen. However, w...
Y Haruhiko, I Sumaru


Studies on the resistance of DMDHEU treated wood against white-rot and brown-rot fungi
2005 - IRG/WP 05-10566
Beech wood (Fagus sylvatica) modified with the textile finishing agent 1,3 dimethylol-4,5-dihydroxyethylen urea (DMDHEU) has shown to improve durability against the white-rot fungus Trametes versicolor. In a mini-block test, the weight loss over eight weeks of incubation decreased with increasing weight percent gain (WPG) of DMDHEU. At 25% WPG, no significant weight loss was observed, while untrea...
P Verma, C Mai, A Krause, H Militz


Bacterial brown stain on sawn timber cut from water-stored logs
1992 - IRG/WP 92-1532
Brown stains which appear on the surface of sawn radiata pine cut from water-stored logs were identified as tannin-like compounds. They are derived from bacterial breakdown of flavanoid-glucosides. When timber is sawn from infected logs, the free flavanoids migrate to the wood surface as the timber dries. There, they condense and oxidise to form permanent brown discolourations. Stains occurred on ...
M E Hedley, R Meder


Quantification of wood decay effects by HPLC analysis
1992 - IRG/WP 92-1576
The present work quantified the effects of the white rot basidiomycetes Coriolus versicolor and Phanaerochaete chrysosporium, and also those of the brown rot fungi Coniophora puteana and Lentinus lepideus, on Pinus patula and Eucalyptus grandis. Wood colonisation was quantified by Kjeldahl nitrogen determinations converted to biomass assays, and degradation was quantified by weight losses produced...
V Singh, M Tarin, G D Shelver, A A W Baecker


The rôle of lignin in the nutrition of several Australian termites
1983 - IRG/WP 1191
The ability of Nasutitermes exitiosus (Hill), Coptotermes acinaciformis (Frogatt), Coptotermes lacteus (Froggatt) and Mastotermes darwiniensis (Froggatt) to degrade 14C-lignin preparations was examined. The lower termites were unable to degrade lignin. Nasutitermes exitiosus was able to cause a 5-8% degradation of hardwood lignins and a synthetic lignin. It failed to degrade the lignin of Pinus ra...
L J Cookson


Influence of CCA and TnBTO on wood decay, under different oxygen levels and various initial moisture content
2001 - IRG/WP 01-10415
Fagus sylvatica (beech) and Pinus sylvestris (Scots pine) were impregnated by TnBTO (tributyl-tin oxide) and CCA (chromated copper arsenate). In wood blocks two levels of moisture content (50% and 100% of saturation point) were used. Three levels of oxygen concentrations (10, 21, and 50%) were chosen. Treated and untreated wood were exposed to three fungi (C. versicolor a white rot, C. puteana, a ...
S M Kazemi, R J Murphy, D J Dickinson


Extracellular hydrogen peroxide producing and hydrogen peroxide reducing compounds of wood decay fungi
1991 - IRG/WP 1516
Extracellular H2O2-producing and H2O2-reducing compounds were isolated from wood-containing cultures of all the white-rot and brown-rot fungi and Ascomycetes which well degraded wood, but were not detected in the culture of the fungi which degraded little wood. The compounds are glycopeptides with a low molecular weight, require H2O2 for one-electron oxidation, catalyze the redox reaction between ...
A Enoki, G Fuse, H Tanaka


A technique for the rapid assessment of wood surface degradation during weathering
1987 - IRG/WP 2281
Radiate pine (Pinus radiata D. Don) sapwood samples taken from four trees were converted into thin strips 100 x 10 x 0.085 (85 µm) mm³, (longitudinal x radial x tangential) in size and exposed in preweighed hatches to the weather inclined at 45° facing equatorially for 10, 20, 40, 60, 80 and 100 days. Significant (P < 0.001) weight losses occurred with time over the exposure period. Differen...
P D Evans, D Abbott, S Thein


Pyrolysis GC-MS as a tool for lignin analysis
1987 - IRG/WP 2201
Pyrolysis-GC-MS is a rapid process for analysis of rather complex organic compounds. The method has great application to petroleum shale oil and wood lignin. Small powdered samples (several mg) are placed in silica capillary tubes and rapidly heated in an inert atmosphere such as He or N2. Breakdown products of the pyrolysis are then trapped and held for an allocated time in an injector attached t...
L Doimo


Control of sapwood-inhabiting fungi by fractionated extracellular metabolites from Coniophora puteana
1991 - IRG/WP 1494
The objective of this study was to test the fractionated metabolites released by Coniophora puteana for their antagonistic activity against the sapstain fungi Ceratocystis coerulescens and Aureobasidum pullulans, and the molds Asperigillus niger and Penicillium spp. The acetone-soluble fraction obtained from the culture filtrate prepared from Coniophora puteana grown on 6% malt extract agar inhibi...
S C Croan, T L Highley


A new approach to the maintenance of wooden railway sleepers. (Final Report)
1992 - IRG/WP 92-3724
The micro-environment of wooden railway sleepers was investigated to assess their condition, to determine the necessary treatment, repair and replacement criteria. In the final report the secondary preservative treatment of wooden sleepers with solid boron rods is discussed; the complete development of an in-situ, non-destructive test method based on structural dynamics analysis is described. The ...
W Beauford, A M Brown, D J Dickinson


Decay resistance of densified ammonia-plasticized stems of oil palm (Elaeis guineensis)
1991 - IRG/WP 3673
When wafers of oil palm stems (Elaeis guineensis) were plasticized with 28% aqueous ammonia and immediately compressed mechanically, the treated material, gained 73% in basic density (average basic density, 0.695 g/cm³), and was highly resistant to decay by wood rot basidiomycetes. Compared with the control specimens (density, 0.403 g/cm³), resistance to decay of the densified specimens by Corio...
A H H Wong, M P Koh


Dimensional lumber model demonstrates the sensitivity of the particle capture immunoassay in early detection of brown-rot fungi
1995 - IRG/WP 95-20058
A dimensional lumber model was designed to test the sensitivity of the particle capture immunoassay (PCI) compared to traditional methods of culturing and microscopic examination for detection of brown-rot fungi. Southern yellow pine 2 x 4&apos;s (5.1x10.2 cm²), and 86.4 cm in length, were inoculated on one end with the brown-rot fungus, Postia placenta, and tested over the full length of the...
C A Clausen, L Ferge


Detection of brown-rot antigens in southern pine
1996 - IRG/WP 96-20090
Brown rot fungal antigens were detected by particle capture immunoassay (PCI) in southern pine 2 x 4&apos;s beyond visible or culturable hyphal growth. Further analysis of test samples revealed changes along the 2 x 4&apos;s that could be grouped into zones. Zone 1, the point of inoculation through 6 cm, showed low pH, measurable oxalic acid, high moisture, and high protein. Zone 2, throug...
C A Clausen


Lignin degradation by a non-enzymatic system supposed to be active in white rot fungi
2000 - IRG/WP 00-10340
Electron microscopic investigations have shown that ligninolytic enzymes of white-rot fungi are only able to penetrate the wood cell wall in late stages of degradation. Thus, the selective degradation of lignin of certain white-rot fungi can only be explained on the basis of a low molecular weight, highly diffusible system. A system, consisting of copper, a coordination compound and either H2O2 or...
P Lamaipis, W Gindl, T Watanabe, K Messner


Hemicellulosic induction of oxalic acid in Postia placenta
1994 - IRG/WP 94-10060
Most studies suggest that enzymes produced by brown-rot fungi are too large to penetrate sound wood structures, even after decay begins. Thus, nonenzymatic agents have been proposed to initiate brown-rot decay. We have reported that the brown-rot fungus Postia placenta MAD-698 initiates a 2-fold decrease in wood pH within 7 days of colonization which is mediated by production of oxalic acid. Strai...
F Green III, M Larsen, T L Highley


The long road to understanding brown-rot decay. A view from the ditch
1995 - IRG/WP 95-10101
Interest in understanding how brown-rot fungi decay wood has received increasing interest in recent years because of a need to identify novel targets that can be inhibited for the next generation of antifungal wood preservatives. Brown-rot fungi are unique in that they can degrade holocellulose (cellulose and hemicellulose) in wood without first removing the lignin. Furthermore, they degrade holoc...
F Green III, T L Highley


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