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Immunolabelling studies on the detection of enzymes during the degradation of wood by Phanerochaete chrysosporium
1988 - IRG/WP 1364
The degradation of lignin in native lignocellulosic substrates by white rot fungi is poorly understood. Biochemical studies have shown the involvement of a number extracellular ligninolytic enzymes released by white rot fungi which are capable of the oxidative conversion of DHP's (lignin model compounds) in vitro, but to date conclusive evidence for occurrence of these enzymes in wood und...
G F Daniel, T Nilsson, B Pettersson


PXTS; A Metal Free Oligomer Wood Preserving System - A Summary of Data To Date
2004 - IRG/WP 04-30350
The world is continually looking for new wood preservative technology, especially to address environmental concerns. Recent changes in the USA have limited the use of arsenical containing formulations to industrial use through a negotiated voluntary cancellation of most residential label uses. PXTS (Polymeric Alkylphenol Polysulfide) offers a brand new technology that is an all organic system, lik...
M H Freeman, D D Nicholas, D Renz, R Buff


Effects of CCA treatment on settlement and growth of barnacles under field conditions
1998 - IRG/WP 98-50116
As part of a field experiment at seven European marine sites evaluating the effects of CCA treatments on non-target marine organisms, panels treated to nominal loadings of 12, 24 and 48 kg/m3 CCA were exposed at marine sites around the coast of Europe. Within two weeks of exposure, a heavy settlement of barnacles took place on the panels exposed intertidally in France. The intensity of settlement ...
S M Cragg, C Brown, R M Albuquerque, R A Eaton, P Goulletquer


Evaluation of decay fungi in Norwegian buildings
2005 - IRG/WP 05-10562
During a period of 2 years and 3 months (1 January 2001 - 20 March 2003) Mycoteam had 3161 consultations in buildings in southern Norway, 1428 revealing damage from decay fungi. One consultation often revealed several occurrences of fungi, and the total number of occurrences of decay fungi was 3434. Thirty-five different species/genera/groups of decay fungi were recorded. During this period brown ...
G Alfredsen, H Solheim, K Mohn Jenssen


Use of the enzyme-linked immunoassay (ELISA) and immunocytochemistry to detect soft rot in preservative treated and untreated wood
1990 - IRG/WP 2347
A polyclonal antibody was raised against mycelial extracts from the soft rot fungus Phialophora mutabilis. In ELISA assays, the antibody showed strong reactions with its own antigen and strong to moderate cross reactions with 6 other Phialophora soft rot species. With the exception of Ceratocystis albida, the antibody gave only weak or negative reactions with 11 other mold, blue stain and rot fung...
G F Daniel, T Nilsson


Influence of the nature of the substrate and the method of inoculation on the degradative activity of soft rot fungi
1998 - IRG/WP 98-20133
The European Standard (ENV-807) determines the threshold of effectiveness of wood preservatives against the action of soft rot Ascomycetes fungi. Its application in the treatment of wood used in the hazard class 4 does not always offer repeatable results, which could be expected in an experimental norm. For this reason, in this paper, we have evaluated the diverse variables that might influence t...
M T De Troya, A M Navarrete, F Rubio, J Jiménez, D Muñoz-Mingarro, C Rodríguez-Borrajo, F Llinares, M Yuste


Some Experiences with Stake Tests at BAM Test Fields and in the BAM Fungus Cellar Part 1: Comparison of Results of Visual Assessments and Determinations of Static Moduli of Elasticity (MOE)
2005 - IRG/WP 05-20319
With examples of routine in-ground stake tests differences are shown in the performance of wood preservatives at the BAM test fields Lehre and Horstwalde and in the BAM fungus cellar. Signs of attack of micro-organisms were assessed visually according to EN 252. Periodical determinations of static moduli of elasticity (MOE) revealed the influence of the attack on the elastic properties of the wood...
M Grinda, S Göller


The influence of cement and calcium compounds on the performance of CCA preservatives
1983 - IRG/WP 3221
The influence of cement and calcium compounds on the durability of untreated and CCA treated wood is considered. Calcium compounds were found to reduce the toxicity of a CCA preservative to a soft rot fungus at copper to calcium ratios of 1:1 and 1:10 using a cellulose filter paper technique. Further studies are outlined and some possible mechanisms by which cement and calcium compounds may affect...
R J Murphy


Efficacy of a novel copper-based organic solvent preservative in laboratory and fungus cellar tests
1995 - IRG/WP 95-30071
The efficacy of Chemicca 4, a novel copper-based organic solvent preservative, was compared with that of other LOSP in pure culture laboratory decay tests and with that of CCA and TBTO in a fungus cellar exposure. It was markedly superior to copper and zinc naphthenates at equivalent metal retentions in laboratory decay tests, and similar in activity to TBTO. After 60 months' fungus cella...
M E Hedley, P N Maynard


Biological variability in the oxalate/oxalate decarboxylase system among five isolates of the wood-degrading fungus Meruliporia incrassate
2006 - IRG/WP 06-10573
The “dry-rot” wood decay fungus Meruliporia incrassata has recently attracted attention, primarily in the western coastal United States, as a particularly destructive pest of building material. Recently, the US Environmental Protection Agency (EPA) has accepted a voluntary withdrawal of the historically effective chromated-copper arsenate (CCA) as a preservative for wood used in residential se...
C Howell, J Jellison


Common Decay Micromorphology “Anomalies” Challenge Current Decay Classification
2006 - IRG/WP 06-10578
Decay micromorphology was studied systematically for diversely preservative treated Pinus radiata and Fagus sylvatica 20 x 20 x 500 mm stakes across 13 in-ground field test sites, during a 6.5 year exposure. New insight into the micromorphology of the early stages of decay enabled new and more detailed interpretation of the mechanism of their formation and suggested that current decay classificati...
R Wakeling


Laboratory Evaluation of Soft Rot Resistance of Non-Durable Lesser-Known Malaysian Hardwoods
2006 - IRG/WP 06-10582
Resistance of six non-durable lesser-known Malaysian hardwoods, Pulai (Alstonia spp.), Rubberwood (Hevea brasiliensis), Ludai (Sapium spp.), Jelutong (Dyera costulata), Gaham Badak (Blumeodendron tokbrai) and Kayu arang (Diospyros spp.) compared with temperate Scots pine sapwood (Pinus sylvestris), were evaluated using the unsterile soil burial (mixed with Chaetomium globosum) versus vermiculite-b...
A H H Wong


Isolation and characterization of hydroxyl-radical-producing glycopeptide genes from the white-rot basidiomycete Phanerochaete chrysosporium
2006 - IRG/WP 06-10588
During wood decay, the white-rot basidiomycete Phanerochaete chrysosporium secretes low-molecular-weight glycopeptides that catalyze a redox reaction between O2 and electron donors to produce hydroxyl radical. This reaction accounts for most of the hydroxyl radical produced in wood-degrading cultures of P. chrysosporium. In combination with phenol oxidases, hydroxyl radical is believed to play a r...
H Tanaka, G Yoshida, Y Baba, K Matsumura, S Itakura, A Enoki


Quantification of methylene bis thiocyanate in wood and its effect on a sapstain fungus Ophiostoma floccosum
2006 - IRG/WP 06-10591
The mobility and concentrations of MBT at different depths of wood billets (200 mm long, 40 mm in diameter) were determined using NMR spectroscopy and ICP-ASE, and then correlated the effect of wood MBT concentrations on growth of O. floccosum in a bioassay using stereomicroscopy and confocal laser scanning microscopy (CLSM). The 13C NMR spectra showed the presence or absence of MBT in wood but w...
T Singh


Molecular characterization of M’jej, decaying agent of cedar forests in Morocco
2006 - IRG/WP 06-10593
Cedar wood is well appreciated since thousand years even though its economical importance is limited because of its world distribution restricted to some Mediterranean countries and Himalaya. The most important species, Cedrus atlantica have its biggest population in Morocco (130 000 ha) where it is submitted to fungal diseases. Among them, the locally named “M’jej” reduced notably saw mile ...
A Zaremski, S Bakkali-Yakhlef, C Chaintreuil, Y Abbas, Y Prin, M Abourouh, M Ducousso


Biological resistance of didecyl dimethyl ammonium tetrafluoroborate (DBF)-treated wood in soil-bed and Basidiomycetes tests
2006 - IRG/WP 06-30393
This study evaluated the decay resistance of treated wood with a new quaternary ammonia compound, didecyl dimethyl ammonium tetrafluoroborate (DBF) in laboratory soil bed and Basidiomycetes tests. Treated sugi sapwood specimens with DBF at various concentration levels were first subjected to soil bed tests using two types of soil. The specimens subjected to soil bed tests were then used for Basidi...
S N Kartal, C Brischke, A O Rapp, Y Imamura


Beetle-Fungus Associations in Woody Substrates in the Context of International Trade
2007 - IRG/WP 07-10610
Interest in the role of insects in transmitting various fungi and in the potential for outbreaks of insects vectoring plant disease organisms has resulted in a large volume of literature on insect-fungi relationships. Mites are mentioned as an important third partner in these associations. This paper summarizes information and provides an update on major aspects of these intricate associations in ...
A Uzunovic


Phenol oxidase activity and one-electron oxidation activity in wood degradation by soft-rot deuteromycetes
2007 - IRG/WP 07-10615
Wood degradation, one-electron oxidation activity as assayed by ethylene generation from 2-keto-4-thiomethylbutyric acid (KTBA), and phenol oxidase activity were measured in cultures of six deuteromyce fungi, with glucose or wood as the carbon source. The four fungi that degraded Japanese beech wood had higher one-electron oxidation activities in wood-containing cultures than in glucose-containing...
H Tanaka, M Yamakawa, S Itakura, A Enoki


Construction of an ITS sequence database for the identification and classification of wood rot fungi
2007 - IRG/WP 07-10626
Developing a database that contains information about wood rot fungi is important in improving technologies about wood preservation as well as advances in understanding wood decay. On the other hand, with rapid spread of the Internet, establishment of a system for the swift precise access to information are required for the computerization of the research information. In the present study, genetic...
S Horisawa, Y Honda, S Itakura, S Doi


White Rot in Eucalyptus Wood: Anatomical Changes and Density Variation by X-Ray Densitometry
2007 - IRG/WP 07-10631
Wood samples of the hybrid Eucalyptus grandis x urophylla attacked by white rot fungi were characterized. Microscopically, the colonization of the fungi was observed in the vessels and radial parenchyma, however the cell walls of the some fibers were attacked, indicating susceptibility to this fungi. Zone lines were observed as a result of interaction and compartmentalization of the fungi. Microsc...
M Tomazello Fo, S Brazolin, M P Chagas


Laser stain removal of fungus-induced stains on paper-based cultural relics
2007 - IRG/WP 07-40389
Laser techniques has been developed and applied in commercial use for 40 years, but it has not been used successfully to remove stains from paper-based cultural relics . The purpose of this study was to investigate the paper cleaning by using a technique of Nd-YAG laser. Artificial stained papers were inoculated with fungi and then were treated at different laser cleaning parameters, in order to f...
Tsang-Chyi Shiah, Han Chien Lin


Detection of Anti-Fungal Sapwood Extractives in Non-Durable Scots Pine (Pinus sylvestris), Rubberwood (Hevea brasiliensis) and Jelutong (Dyera costulata)
2007 - IRG/WP 07-10634
A general laboratory bioassay method of Woodward and Pearce (1985) was adopted to detect anti-fungal activity of sapwood or heartwood extractives of 5 Malaysian hardwoods [dark red meranti heartwood (Shorea spp.), red balau heartwood (Shorea spp.), kulim heartwood (Scorodocarpus borneensis), jelutong sapwood (Dyera costulata) and rubberwood sapwood (Hevea brasiliensis), including the temperate Pin...
A H H Wong, R B Pearce


Effect of fiber type and content on the natural durability of wood flour/high density polyethylene composites against rainbow fungus (Coriolus versicolor)
2007 - IRG/WP 07-40387
In order to evaluate the effect of fiber type and content on the natural durability of wood flour/high density polyethylene (HDPE) composites against Coriolus versicolor, samples containing 25% and 50% by weight of various natural fibers and HDPE were selected. Natural fibers included in the study were wood flour, rice hulls, hemp fibers and newsprint. Samples containing 25% and 50% natural fiber ...
A Karimi, M Tajvidi, S Pourabbasi


Effect of Compatibilizer on the Natural Durability of Wood Flour/High Density Polyethylene Composites Against Rainbow Fungus (Coriolus versicolor)
2007 - IRG/WP 07-40388
To evaluate the effect of compatibilizer on the natural durability of wood flour/high density polyethylene composites against Coriolus versicolor, composites containing 25% and 50% by weight maple wood flour and 1% and 2% compatibilizer (Maleic anhydride polyethylene (MAPE)), respectively, were sampled. Identical specimens of the same composites without the compatibilizer were also prepared. ...
Sara Pourabbasi, A Karimi, D Parsapajouh, M Tajvidi, M Soleymani


Biological control of the sapstain fungus Diplodia pinea (syn. Sphaeropsis sapinea) in the field
2008 - IRG/WP 08-10663
Diplodia pinea, syn. Sphaeropsis sapinea, is well known as the worldwide ocurring causal agent of the Diplodia tip-blight disease on various pine species, leading to great losses of valuable wood in the forests. Moreover, this fungus is also responsible for the fornation of sapstain of felled logs, leading to undesired disation of surface layers in the OSB manufacturing industry. In Central Europe...
C Gradinger, T Boisselet, K Messner, K Fackler


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