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Natural durability of larch heartwood against decay
1997 - IRG/WP 97-10201
Larch heartwood has been promoted in Sweden and in other European countries as an alternative to preservative treated timber. It has even beeen stated that larch heartwood is more durable than preservative treated timber. This paper presents a compilation of facts from scientific reports on the durability of larch heartwood. Some recent data from this department are also reported. All data suggest...
T Nilsson


Solid state NMR study on pine wood degraded by brown rot fungus Gloeophyllum trabeum
1994 - IRG/WP 94-10067
Blocks of Pinus koraiensis wood were degraded by Gloeophyllum trabeum and examined by solid-state 13C NMR spectroscopy. Chemical compositions were estimated from relative signal aras. There was no evidence for preferential degradation of the noncrystalline cellulose which accounted for about 4% by weight of the wood. Loss of partly ordered cellulose on crystal surfaces exceeded the loss of crystal...
Yoon Soo Kim, R Newman


New ways of chemical wood preservation
1997 - IRG/WP 97-40087
Synergistic effects pertaining to wood preservation have been investigated by combining different polymerizing agents with modifying chemicals, modifying chemicals with known preservatives and new types of preservative chemicals with each other. One of the investigated methods is the use of impregnating solutions based on furfuryl alcohol (produced from biomass), in which the chemicals polymerizes...
M Westin, T Nilsson, B Ohlsson, R Simonson


A possible role of unique TCA cycles in wood-rotting basidiomycetes
2003 - IRG/WP 03-10461
The copper tolerant brown-rot fungus, Fomitopsis palustris, acquires metabolic energy by use of the constitutively-occurring Kornberg’s glyoxylate cycle coordinating with oxalate biosynthesis and glucose oxidation (Erman Munir et al. Proc. Natl. Acad. Sci. USA, (2001) 98, 11126-11130). Furthermore, this fungus does not have the normal TCA cycle, lacking 2-oxoglutarate dehydrogenase which is a ke...
E Munir, T Hattori, M Shimada


Evaluation of the natural durability and ultrasonic method for decay detection of some european hardwood and softwood species
2004 - IRG/WP 04-10537
This paper presents the first part of an investigation on the natural durability of mixed plywood made of durable and non durable plies from the following selected timbers : Cedrus atlantica, Cupressus sempervirens, Castanea sativa, Populus sp. I 214 and Fagus sylvatica. In order to carry out this study, the natural durability of the massive wood used to manufacture the plywood panels was asses...
F Faraji, M-F Thévenon, B Thibaut


Application of non-destructive techniques (durometric and ultrasonic) to evaluate the degradation of woods in service by Gloeophyllum trabeum
2001 - IRG/WP 01-20220
In order to establish the degree of degradation of wood used in construction, it is necessary to employ non-destructive methods (ultrasonic, durometric, densitometric). In this study, ultrasonic and Pilodyn durometric techniques have been applied to try to establish parameters of relationship between the values obtained by both, for their immediate application to wood in service. As a method of re...
M T De Troya, L Palaia, A Navarrete, V Galvañ, R Molina, A Guijarro, J Camacho


Characterization of Poria indoor brown-rot fungi
1995 - IRG/WP 95-10094
The heterogeneous group of "Poria" fungi causing brown rot in buildings and also of wood in ground contact comprises Antrodia vaillantii, Antrodia serialis, Antrodia sinuosa, Antrodia xantha and Tyromyces placenta. These fungi have similar morphological appearance and biology. Their nomenclature has a confusing history and is still not uniform. As a consequence, misinterpretations may occur. SDS p...
O Schmidt


Natural Durability studies and Changes in wood chemistry of some Ghanaian hardwoods during decay by white- and brown-rot fungi
2004 - IRG/WP 04-10542
The natural decay resistance of Esa (Celtis mildbraedii), Wawabima (Sterculia rhinopetela) and Dahoma (Piptadeniastrum africanum) obtained from Ghana was determined according to BS EN 113 (1997). Esa was found not durable to the white-rot, but very durable to the brown-rot; Wawabima was very durable to both white- and brown-rots; and Dahoma was durable to the white-rot, and very durable to the bro...
Zeen Huang, K Maher, S A Amartey


Efficacy of waterborne copper naphthenate as preservative for northern hardwood species
1995 - IRG/WP 95-30091
Northern red oak (Quercus rubra) and red maple (Acer rubrum) cubes pressure treated with waterborne formulation of copper naphthenate were evaluated for resistance to attack by brown fungi Gloeophyllum trabeum (Pers. ex Fr.) Murr. (Madison 617 ATCC 11539) and Poria placenta (Fr.) Cooke (Madison 698, ATCC 11538), white rot fungi Trametes versicolor (L. ex Fr.) Pilat (R-105 from Jeff Morrell), Irpex...
D P Kamdem, R Fair, M H Freeman


Colonization of treated and untreated ponderosa pine exposed in Hilo, Hawaii
1995 - IRG/WP 95-20068
The rate of decay in above ground exposures is largely controlled by rainfall and temperature, factors which can be used to construct a climate index of decay hazard in above ground exposures. Developers of new biocide formulations have utilized this knowledge by establishing test sites in sub-tropical regions such as the Gulf Coast of the United States. More recently, field sites have been locate...
C M Freitag, J J Morrell, K J Archer


Silicic acid-Boric acid complexes as wood preservatives
2001 - IRG/WP 01-30273
A silicic acid monomer aqueous solution (SAMS) or colloidal silicic acid solution (CSAS) was combined with various metal compounds or boric acid. Agents where SAMS or CSAS was combined with boric acid gave good protection against decay caused by the brown-rot fungus Fomitopsis palustris, the treated wood (Cryptomera japonica D. Don) specimens after the leaching test maintained a high resistance to...
H Yamaguchi


The production of extracellular hydrogen peroxide by some brown-rot fungi
1990 - IRG/WP 1446
The role of hydrogen peroxide (H2O2) has been discussed in the degradation of wood by wood-rotting fungi. The production of extracellular hydrogen peroxide was studied by detecting the oxidation of the chromogen 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) by H2O2 and horse radish peroxidase (HPR). ABTS and HPR were added to a solid wood based culture media. In this study ...
A-C Ritschkoff, L Paajanen, L Viikari


Immunolocalization of extracellular metabolites from Poria placenta
1988 - IRG/WP 1361
Polyclonal antisera produced to Poria placenta extracellular metabolites was used in immuno-fluoresence microscopy and immuno-gold TEM studies. In the fluorescence work, labelling of Poria placenta hyphae in wet fixed wood material was observed but not in infected wood which was oven dried prior to sectioning and immunolabelling. TEM studies provided better resolution, with gold labelling detected...
B Goodell, G F Daniel, J Jellison, T Nilsson


Cation analysis of wood degraded by white and brown rot fungi
1992 - IRG/WP 92-1552
Changes in the concentrations and composition of cations within wood colonized by the brown-rot fungi Postia placenta and Gloeophyllum trabeum and the white-rot fungi Trametes versicolor and Phanaerochaete chrysosporium were analyzed using plasma emission and atomic absorption spectrophotometry. In degraded poplar wood significant decreases in pH and electrical resistance readings were observed as...
J Jellison, K C Smith, W T Shortle


Method of embedding and staining of wood after biological testing to support the identification of decay type
1998 - IRG/WP 98-20131
A method for preparing wood for microscopic analysis after decay testing is described, with special regard to a novel fast simultaneous staining, which helps to identify different types of decay. The novel staining process is explained as well as the embedding of heavily decayed samples in polyethylene glycol (PEG) and picking up of sections by transparent adhesive tape. Special feature of the int...
A O Rapp


Occurrence and importance of various types of fungal and bacterial decay in CCA-treated horticultural pine posts in New Zealand
1984 - IRG/WP 1234
A detailed microscopical examination has been carried out on samples taken from CCA-treated pine posts exposed in horticultural soils. The following decay types were observed: white rot, brown rot, soft rot, tunnelling bacteria, cavitation bacteria, and bacterial erosion. The occurrence and importance of the various decay types between different regions, and plots within a specific region, varied ...
T Nilsson


Extracellular osmiophilic particles in connection with brown rot and white rot
1982 - IRG/WP 1157
The way of attack on Pine woodblocks of two brown-rot fungi (Fomitopsis pinicola, Coniophora puteana) differing in their cellulase activities, and one white-rot fungus (Trametes hirsuta) has been investigated by transmission electron microscopy. Even at early stages of decay, osmiophilic particles were found with all the fungi investigated. In the brown-rotted wood, the osmiophilic particles occur...
K Messner, H Stachelberger


Production, function and neutralization of oxalic acid produced by the dry rot fungus and other brown rot fungi
1987 - IRG/WP 1330
The formation of oxalic acid by the wood-destroying fungi causing brown rot, is found to be the key which by hydrolysing the hemicellulose brings the cellulose in the tracheid wall in contact with the cellulase enzymes and yeld watersoluble sugars leaving only a lignin skeleton. To control the pH in the substrate the excess oxalic acid is precipitated to water insoluble calcium oxalate by the dry ...
J Bech-Andersen


The role of oxygen and oxygen radicals in one-electron oxidation reactions mediated by low-molecular weight chelators isolated from Gloeophyllum trabeum.markup
1994 - IRG/WP 94-10086
The KTBA assay for determination of one-electron oxidation activity was used to assay reactions of low-molecular weight chelators isolated from the brown rot fungus Gloeophyllum trabeum. The assay, performed either under air or nitrogen showed that molecular oxygen was an important factor in chelator-mediated oxidation reactions. A reduction in oxidative activity was observed when superoxide dismu...
Jun Lu, B Goodell, Jiang Liu, A Enoki, J Jellison, H Tanaka, F Fekete


Hydrolysis of bordered pits during colonization of conifers by brown-rot fungi
1995 - IRG/WP 95-10103
Brown-rot decay results in rapid reduction in degree of polymerization (DP) of holocellulose with concomitant strength loss (MOR) without removing lignin. Development of new methods of wood protection will require focusing on early events in the sequence of depolymerization. Bordered pit membranes (sapwood) represent a readily available source of non-lignified carbohydrate, ie. pectin and cellulos...
F Green III, J L Tschernitz, T A Kuster, T L Highley


Antagonistic properties of Gliocladium virens against wood attacking fungi
1996 - IRG/WP 96-10162
Gliocladium virens has shown good antagonism against decay fungi in agar medium and in wood blocks. Gliotoxin produced by Gliocladium virens is associated with biocontrol of some plant diseases, but its importance to biocontrol of wood-attacking fungi is unknown. We investigated the ability of gliotoxin-producing (GLT+) isolates of Gliocladium virens and gliotoxin-deficient (GLT-) mutants of Glioc...
T L Highley, H S Ananthapadmanabha, C R Howell


Micromorphology of oak wood degraded by brown rot fungus Coniophora puteana
2000 - IRG/WP 00-10356
To characterize the degradation of hardwood by brown rot fungus, Coniophora puteana was incubated on the wood blocks of Quercus accutissima. For comparison, the same fungus was also incubated on the pine wood blocks of Pinus densiflora. In softwood, the shape of the wood cells and thickness of the cell appeared unchanged even the loss of birefringence in the affected areas. In contrast, oak wood d...
Yoon Soo Kim, Seung-Gon Wi, Kwang-Ho Lee


Oxalic acid metabolism of Postia placenta
1992 - IRG/WP 92-1566
The oxalic acid metabolism of Postia placenta was studied in liquid culture in order to identify the metabolic pathways of biosynthesis and to determine whether oxalic acid physiology could be correlated with efficiency in wood decay. Oxalic acid production was determined for test strain MAD698 grown in a basal defined medium with a variety of different carbohydrate and nitrogen sources. The highe...
J A Micales


Immuno-scanning electron microscopic localization of extracellular polysaccharidases within the fibrillar sheath of the brown rot fungus Postia placenta
1991 - IRG/WP 1497
Extracellular polysaccharidases of the brown-rot fungus Postia placenta were localized using colloidal gold labeled monoclonal antibodies to the B-1,4-xylanase (32-36kDa) fraction of Postia placenta. Postia placenta was grown from agar onto glass coverslips, immunolabeled with or without prior fixation, and examined by SEM. Enzymes were localized on the hyphal surface and on the clumped fibrillar ...
F Green III, C A Clausen, M J Larsen, T L Highley


The effect of Tween 80 on the growth, morphology, and enzyme secretion of Postia placenta
1990 - IRG/WP 1456
The nonionic surfactant Tween 80 (polyethylene oxide sorbitan mono-oleate) has been reported to increase enzyme production and/or secretion in bacteria and fungi. Such a procedure could greatly facilitate research into the physiology of wood-decay fungi since quantities of available enzyme are often limiting. The brown-rot fungus Postia placenta was grown in a synthetic medium supplemented with 0,...
J A Micales


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