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An unusual soft-rot decay pattern caused by the Ascomycete Hypoxylon mediterraneum (de Not.) J Miller
1984 - IRG/WP 1222
A distinct pattern of soft-rot decay has been observed for the fungus Hypoxylan mediterraneum (de Not.) J. Miller. This fungus also produced decay patterns typical of brown and white rot decay. The production of characteristic cavities by Hypoxylan mediterraneum was prolific in the hardwoods Eucalyptus maculata and Eucalyptus regnans, but infrequent in the softwoods, Pinus elliottii and Pinus radi...
D M Francis, L E Leightley


Use of immunoblotting for the analysis of wood decay basidiomycetes
1988 - IRG/WP 2307
Immunoblotting methods, in particular dot and Western blotting have been used to investigate features of a variety of wood decay organisms, in particular Coriolus versicolor, Lentinus lepideus and Serpula lacrymans. Antisera to each of these decay organisms has been produced by immunisation of rabbits with liquid culture grown hyphae. These antisera, after appropriate preabsorption with sawdust ha...
J W Palfreyman, H Glancy, D Button, A Vigrow, A J Score, B King


Chemical analysis of wood waste - The problem of sampling
1999 - IRG/WP 99-20168
A quantitative analysis of the content of active substances in wood is often demanded in quality control after processing and for the proper waste management. As a measure for quality control, analysis has the advantage that the type of preservative is known and assumptions can be made about its distribution in the treated wood.W aste wood may have been treated by a wide range of organic and inorg...
A Peylo, R-D Peek


An analysis of the effects of some factors on the natural durability of pine (Pinus sylvestris L.) and spruce (Picea abies Karst.)
1986 - IRG/WP 1279
The effects of some factors on the natural resistance of pine and spruce sapwood against fungal decay and against attack of house-longhorn beetle larvae have been studied in laboratory tests and the results are evaluated by analysis of variance and regression analysis. Following conclusions were reached: Wood from summer-felled trees did not have a lower inherent natural durability against fungal ...
J B Boutelje, T Nilsson, S Rasmussen


Le pin: Matériau à contact alimentaire [Pine wood as a material for food contact]
1995 - IRG/WP 95-50040-01
Monoterpenes emitted to air from woods of pine (Pinus nigra, Pinus laricio, Pinus pinaster and Pinus sylvestris) were determined on the one hand by static analysis and by other hand by sampling on the Tenax adsorbent, followed by analysis using thermal desorption, both being combined with gas chromatography coupled with mass spectrometric detection. The major monoterpenes were alpha-pinene and 3-c...
A Waymel, M Lamour, G Labat


The action of siderophores isolated from Gloeophyllum trabeum on the structure and crystallinity of cellulose compounds
1991 - IRG/WP 1479
Low molecular weight, high affinity iron-binding compounds (siderophores) were isolated from the brown-rot fungus Gloeophyllum trabeum. The compounds were shown to be inducible by iron starvation and could be purified by ultra-filtration, ethyl acetate extraction, column chromatography and preparative HPLC. The isolated compounds were shown by analytical and immunological techniques to be produced...
J Jellison, V Chandhoke, B Goodell, F Fekete, N Hayashi, M Ishihara, K Yamamoto


Effect of fungal degradation on the chemical composition of acetylated beech wood
2003 - IRG/WP 03-40267
This study investigated the impact of fungal attack on the chemical composition of acetylated wood. Beech wood acetylated to different degrees was exposed to decay by the white-rot fungus Trametes versicolor under solid-state fermentation conditions. Laboratory soil-bed assays were also conducted to study the degradation of acetylated wood by soft rot fungi and other soil-inhabiting microorganisms...
H Militz, Dong-won Son, L Gómez-Hernández, R Sierra-Alvarez


An investigation of the nutritional physiology of the wood-boring weevil Euophryum confine Broun
1994 - IRG/WP 94-10082
The digestion of Picea abies Karst and Pinus sylvestris L. by Euophryum confine, at two sites is reported in this study. The composition of sound wood, wood from the heads of the weevil tunnels and the frass egested has been determined by gravimetric analysis. A comparison of sound wood and wood ingested showed that weevils tunnelled in microbially decayed wood, while a comparison of the wood inge...
A J Pitman, S M Cragg, G S Sawyer


The micro-distribution of copper/chrome/arsenate in Acer pseudoplatanus and Eucalyptus maculata
1973 - IRG/WP 319
The excellent field performance of copper-chrome-arsenate (CCA) treated timber has been accepted for many years. The preservative loadings used in practice have been based on field trial results, backed by service tests. The performance of treated hardwoods in trials and practice indicated that provided the required loading and penetration could be achieved the performance would be good. Recent un...
D J Dickinson


The extraction of boron from treated wood for quantitative analysis: A comparison of procedures
1992 - IRG/WP 92-2414
The extraction of boron preservative from treated rubberwood and scots pine samples was compared using methods based on refluxing and extraction in 1N NaOH or distilled water at 80°C. The extract solutions were analysed by Inductively Coupled Plasma spectroscopy (ICP/AES). A simple extraction procedure based on immersion of wood samples of approximate dimension 2 x 1 x 1 cm³ in distilled water a...
R J Murphy, D J Dickinson, P W McCormack, M K Lung


Stability of bifenthrin in a commercial phenol-formaldehyde plywood glue
2003 - IRG/WP 03-30311
Liquid phenol formaldehyde (PF) glue mixes used for plywood manufacture are strongly alkaline. At this pH insecticidal additives may not be stable for long periods. In order to establish practical working life of the synthetic pyrethroid insecticide, bifenthrin, in liquid PF glues the concentration of bifenthrin in the glue mix was measured under laboratory conditions over a 24 hour period. Glu...
M J Kennedy, P A Collins, R D Vella


Examination of power poles by computerized tomography
1980 - IRG/WP 2142
The technique known as computed axial tomography (CAT or CT) has become widely known because of the success it has achieved in medical imaging and diagnostics. It has now been used to give non-destructive crosssectional pictures of a variety of industrial objects, including wooden power poles, with a spatial resolution of about 1 mm (0.04 in.). It is obvious the benefits that would be derived from...
J A Taylor, I L Morgan, H Ellinger


Analysis of the boron content of preservative treated oak and pitch pine heartwood before and after leaching
1992 - IRG/WP 92-3697
Studies to determine the effects, if any, of a variety of boron formulations on heartwood of English oak and American Pitch pine have been previously reported (King et al (1991)). Small wood blocks were treated, at 10°C or 45°C, with borax, polybor or Boracol 20 for periods of 1 month or 4 months then analysed or continuously leached with tap water for a period of 1 month. Reported results showe...
S McCutcheon, G M Smith, J W Palfreyman, B King


Detection of the dry rot fungus Serpula lacrymans by amplified ribosomal DNA restriction analysis
1998 - IRG/WP 98-10245
Isolates of the dry rot fungus Serpula lacrymans and the wild merulius S. himantioides were investigated by amplified ribosomal DNA restriction analyses (ARDRA) of the internal transcribed spacers (ITS). The technique uses the polymerase chain reaction (PCR) to amplify the variable region of the ITS between the conserved 18S and 28S genes of the nuclear ribosomal DNA (rDNA). The ITS region of all isolates of Serpula lacrymans and S. himantioides was successfully amplified. The length of the amplified product was about 630 bp. Subsequent double digest of the ITS with the restriction endonucleases Haelli and Taql separated the closely related fungi by species-specific fragments. Thus, ARDRA-ITS proved to be suited for the detection of the dry rot fungus.
O Schmidt, U Moreth


Analysis of the degradation of carbohydrates by blue-stain fungi
1990 - IRG/WP 1457
The topic of the degradative ability of blue-stain fungi literature is not clear. Many authors support the idea that those fungi don't have enzymatic capability to decay the wall of the ligneous cells. However, others have found some decay activity. In this study, we have attempted to analyze which wall cells soluble carbohydrates are metabolized by some blue-stain fungi, found in Spain. ...
M T De Troya, A M Navarrete, E Relano


Conforming to European standards on preservative treatment
1998 - IRG/WP 98-20150
New European standards require that specifications for the preservative treatment of timber are written in terms of the penetration and retention of preservative within the treated commodity that result from the treatment process. In order to check compliance with this type of specification, suitable methods of quantitative analysis must be available. This paper presents the results of a study by ...
E D Suttie, R J Orsler


Amenability of radial permeability of Sitka spruce (Picea sitchensis (Bong.) Carr.) as affected by aspects of cell ends in uniseriate ray parenchyma tissue
2003 - IRG/WP 03-40252
The principal objective of this article was to recognise and understand the amenability of ray parenchyma cell ends (end platform) in uniseriate ray tissue to influence the radial permeability of Sitka spruce (Picea sitchensis (Bong.) Carr.) by examination of most (Queen Charlotte Islands in Rhondda, South Wales) and the least (South Oregon in Dalby, North-East England) seed origins that grown in ...
I Usta, M D C Hale


A comparison analysis of eight strains of Serpula lacrymans (Schum. ex Fr.) S.F. Gray
1991 - IRG/WP 2362
Investigations were previously carried out to compare eight strains of Serpula lacrymans (Schum. ex Fr.) S.F. Gray (some used in various countries as standard test strains): FPRL 12C (England), FPRL 12E (Germany), Warsaw III (Poland), HFP 7802 (Japan), DFP 16508, 16509, 16521 and 16522 (Australia). Studies included growth rate and dry mass of mycelium, decay capacity, reduction of compression stre...
J Wazny, J D Thornton


Interactions between soft rot fungi and CCA preservatives in Betula verrucosa
1988 - IRG/WP 1367
Ultrastructural investigations were carried out to obtain information on the cell wall distribution of CCA elements during soft rot decay of 4% K33 CCA preservative treated birch (Betula verrucosa) wood. TEM observations on fibres at various stages of S2 cavity formation by mutabilis showed a distinct pattern of electron dense materials to occur within the S2 cell wall region. These materials (gra...
G F Daniel, T Nilsson


Leaching from field test stakes. Results from two different methods of analysis
1993 - IRG/WP 93-50013
Field test stakes treated with Boliden K33 containing copper, chromium and arsenic exposed at three different fields in Sweden and rejected due to decay, have in two studies been analysed regarding leaching and remaining preservatives. The stakes have been exposed during 20 to 43 years. The density of the stakes and content of copper, chromium and arsenic have been determined in the top part of th...
F G Evans, M-L Edlund


Quantification of methylene bis thiocyanate in wood and its effect on a sapstain fungus Ophiostoma floccosum
2006 - IRG/WP 06-10591
The mobility and concentrations of MBT at different depths of wood billets (200 mm long, 40 mm in diameter) were determined using NMR spectroscopy and ICP-ASE, and then correlated the effect of wood MBT concentrations on growth of O. floccosum in a bioassay using stereomicroscopy and confocal laser scanning microscopy (CLSM). The 13C NMR spectra showed the presence or absence of MBT in wood but w...
T Singh


Direct analysis from wood of the blue stain fungi Aureobasidium pullulans and Hormonema dematioides by denaturing gradient gel electrophoresis
2006 - IRG/WP 06-10595
Aureobasidium pullulans and Hormonema dematioides are the two organisms used in the EN 152 laboratory method for determining the effectiveness of preservatives against blue stain in service. The literature concerning the disfigurement of surface coatings and exposed timber in-service states that A. pullulans is the dominant blue stain fungus, due to its frequent isolation from stained material. In...
M J Ray, D J Dickinson


Time-of-Flight Secondary Ion Mass Spectrometry Analysis of Polyethylene Glycol Penetration into Wood Cell Walls
2006 - IRG/WP 06-20328
Penetration of high molecular weight polyethylene glycol (PEG) into wood cell walls was investigated in red pine. PEG of different molecular weights (1000, 8000 and 20000) was labelled by bromination and treated samples were analyzed on Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) in dynamic mode. Both negative and positive spectra of the samples were collected in high spatial (bunche...
D Jeremic, P Cooper, P Brodersen


Effects of chlorothalonil (CTN) and butylated hydroxytoluene (BHT) on microbial communities involved in the deterioration of wood using T-RFLP I: Accelerated laboratory decay study
2006 - IRG/WP 06-20332
The effects of Chlorothalonil (CTN) and Butylated Hydroxy Toluene (BHT) on microbial species diversity in wood and the surrounding soil are being assessed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). CTN was selected as a trial organic wood preservative, and BHT is being evaluated for its synergistic effects with CTN. Results from an accelerated decay test will be presented. The...
G T Kirker, M L Prewitt, S V Diehl


Water analysis in chemicals, wood or chemically treated wood using a new analytical method for selective water detection
2006 - IRG/WP 03-30391
Water or moisture content analysis can be a challenging task in many applications. However, knowing accurately the type of water which can be found in a given sample or product can provide important information about a chemical compound used for wood treatment and especially about the treated wood sample. Moisture content in wood plays an important role in fungal growth and it needs to be extensi...
R Craciun, D Taylor


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